scholarly journals Uji Efektivitas Ekstrak Buah Jeruk Nipis (Citrus aurantifolia) dalam Menghambat Pertumbuhan Bakteri Enterococcus faecalis Secara In Vitro

2015 ◽  
Vol 3 (2) ◽  
pp. 111-118
Author(s):  
Annisa Fajriatul Arafah ◽  
Vivi Triana ◽  
Murniwati Murniwati
Keyword(s):  
Citric Acid ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Bacterial Cell ◽  
Early Stage ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Control Group ◽  
Citrus Aurantifolia

Enterococcus faecalis is a normal microorganism in root canal teeth, anaerobic facultative, and gram positive cocci. This bacteria is opportunistic that cause secunder infection in root canal. Lime (Citrus aurantifolia) is one of the plants which have antimicrobial activity because lime contains flavonoid and citric acid. Flavonoids can denature proteins in bacterial cell and damaging the cell membrane. Citric acid can damaging the bacterial cell wall and inhibiting bacterial enzymes activity. The purpose of this study was to examie the effectiveness lime extract in various concentration in inhibiting Enterococcus faecalis in in-vitro. The research method was experimental laboratories with posttest only control group design. Samples were used in this research are Enterococcus faecalis bacteria in M. Djamil’s Hospital Microbiology Laboratory, Padang. This research was conducted at organic chemistry of natural materials laboratory FMIPA UNAND and Kopertis Wilayah X Padang laboratory. Early stage was done by making extracts of lime with concentrations are 25%, 50%, 75%, and 100%. Then, the extracts was tested into the bacteria with the diffusion method used papper disk. The inhibition zone formed between lime extracts in various concentrations to the growth of Enterococcus faecalis were calculated using calipers. Data was analyzed by using Kruskal Wallis test and Mann-Whithney test with confidence interval of 95%. The result presented that 25%, 50%, 75%, and 100% lime extracts have inhibition activity in inhibiting the growth of Enterococcus faecalis, and the higher the concentration of the extracts, the greater the inhibition zone formed.

scholarly journals Uji Daya Hambat Ekstrak Kulit Langsat (Lansium parasiticum) Terhadap Pertumbuhan Bakteri Enterococcus faecalis Secara In Vitro

2020 ◽  
Vol 8 (2) ◽  
pp. 70-74
Author(s):  
Andhini Ardi ◽  
Dedi Sumantri ◽  
Surma Adnan
Keyword(s):  
Enterococcus Faecalis ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Control Group ◽  
Fruit Peel ◽  
Control Group Design ◽  
Inhibition Effect ◽  
Facultative Anaerobic Bacteria ◽  
Peel Extract

Enterococcus faecalis is a bacteria that commonly found in secondary root canal infections. Enterococcus faecalis is a gram-positive, facultative anaerobic bacteria. Langsat (Lansium parasiticum) is a natural ingredient that contains secondary metabolites and has antibacterial ativity. The aim of this study was to examine the inhibition effect of langsat fruit peel extract (Lansium parasiticum) to the growth of Enterococcus faecalis. This was a laboratory experimental study with post-only control group design. Langsat fruit peel extract with 25%, 50%, 75%, and 100% concentration was using disc diffusion method on Mueller-Hinton Agar (MHA) and the inhibition zone was measured with a sliding caliper. The data were analyzed with Kruskal-Wallis test and Mann-Whithney test. The result of this study was langsat fruit peel extract with 25%, 50%, 75% concentration showed a inhibition zone, while at 100% showed no inhibition zone. The Kruskal-Wallis test showed that there are significant differences (p <0.05) between all groups. The conclusion was langsat fruit peel extract (Lansium parasiticum) have weak inhibition effect of the growth of Enterococcus faecalis bacteria. The inhibition zone of concentrations 25% : 2,20 mm;  50%: 0,94 mm; 75%: 0,36 mm and 100% : 0,00 mm.

scholarly journals Uji daya hambat ekstrak biji kopi robusta (Coffea robusta) terhadap pertumbuhan Enterococcus faecalis secara in vitro

e-GIGI ◽  
2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Vilani A. M. Tilaar ◽  
Marie M. Kaseke ◽  
Juliatri .
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Seed Extract ◽  
Diffusion Method ◽  
Control Group ◽  
Robusta Coffee ◽  
Post Test ◽  
Coffea Robusta ◽  
Coffee Seed

Abstract: Enterococcus faecalis is commonly found in the root canal especially in failing treated root canal. These bacteria is resistant against many antibacterials. Recently, natural antibacterials have been developed as alternative antibacterials particularly for oral infection such as Robusta coffee seed extract (Coffea robusta) which contains antibacterial compounds (caffeine, volatile acid, and phenol). This study aimed to obtain the inhibition ability of Robusta coffee seed extract against growth of Enterococcus faecalis in vitro. This was a true experimental study with a post test only control group design. This study used agar diffusion method with well modified technique with 5 times of repetitions. The positive control was chlorhexidine 2% and the negative control was aquades. The results showed that Robusta coffee seed extract could inhibit the growth of Enterococcus faecalis with an average diameter of inhibition zone of 13.8 mm.Keywords: enterococcus faecalis, coffea robustaAbstrak: Enterococcus faecalis sering ditemukan di dalam saluran akar terutama yang gagal terhadap perawatan. Bakteri ini telah resisten terhadap banyak bahan antibakteri. Saat ini telah banyak dikembangkan bahan antibakteri alami sebagai alternatif pengobatan antibakteri khususnya untuk infeksi rongga mulut, yaitu antara lain kopi robusta (Coffea robusta) yang mengandung senyawa antibakteri (kafein, asam volatil, dan fenol). Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak biji kopi robusta terhadap pertumbuhan bakteri Enterococcus faecalis secara in vitro. Jenis penelitian ialah eksperimental murni (true experimental design) dengan post test only control grup design. Penelitian ini menggunakan metode difusi agar dengan teknik sumuran. Pengulangan dilakukan sebanyak lima kali. dengan kontrol positif chlorhexidine 2% dan kontrol negatif aquades. Hasil penelitian menunjukkan bahwa ekstrak biji kopi robusta (Coffea robusta) memiliki daya hambat terhadap pertumbuhan bakteri Enterococcus faecalis dengan rerata diameter zona hambat 13,8 mm.Kata kunci: Enterococcus faecalis, Coffea robusta

scholarly journals Perbandingan Efektivitas Antibakteri antara Ekstrak Metanol Kulit Batang Kasturi dengan Ampisilin terhadap Staphylococcus aureusin Vitro

2016 ◽  
Vol 12 (1) ◽  
pp. 1
Author(s):  
M. Rizki Valian Akbar ◽  
Lia Yulia Budiarti ◽  
Edyson Edyson
Keyword(s):  
Staphylococcus Aureus ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
Study Data ◽  
Diffusion Method ◽  
Bark Extract ◽  
Control Group ◽  
Tract Infections ◽  
Post Hoc

Abstract:Kasturi as a typical plant in South Kalimantan is one fruit that has many benefits. The barks of kasturi has proved to have benefits, especially to inhibit the activity of Staphylococcus aureus. Staphylococcus aureus is a gram positive bacteria that cause pneumonia, mastitis, and urinary tract infections. The bark of kasturi can be used in extract form. The objective of this research is to know the difference between the preparations of inhibition kasturi’s bark extract and ampicillin 30μg in inhibiting the growth of Staphylococcus aureus in vitro. This study used an experimental method consisting of 9 treatments with 3 repetitions. Treatment test in the methanol extract of the bark of kasturi 25%, 37.5%, 50%, 62.5%, 75%, 87.5% and 100%. The control group used ampicillin and 70% methanol. Bacterial test was done by using a diffusion method. The parameter measured was the amount of inhibition zone (mm) which grown on media MH. Analysis of study data used One way Annova test and Post Hoc LSD test at α=0,05. The results showed that there were significant differences between the treatment kasturi’s bark extract 25%, 50%, 62.5%, 75%, 87.5% and 100% concentration different compared to ampicillin. Meanwhile, at the concentration of 37.5% (p <0.05) was not significant. The antibacterial effectiveness was obtained from the concentration of 100%. Key words: the bark of kasturi, methanol extract, Staphylococcus aureus, inhibition zone. Abstrak: Kasturi sebagai salah satu tanaman khas yang ada di Kalimantan Selatan merupakan salah satu buah yang memiliki banyak khasiat. Kulit batang kasturi terbukti memiliki manfaat terutama dapat menghambat aktivitas Staphylococcus aureus. Staphylococcus aureus merupakan bakteri gram positif yang menyebabkan penyakit pneumonia, mastitis, dan infeksi saluran kemih. Kulit batang kasturi dapat digunakan dalam bentuk ekstrak. Tujuan penelitian ini mengetahui perbedaan daya hambat antara sediaan ekstrak kulit batang kasturi dengan ampisilin 30µg dalam menghambat pertumbuhan bakteri Staphylococcus aureus secara in vitro. Penelitian ini menggunakan metode eksperimental yang terdiri dari 9 perlakuan dengan 3 kali pengulangan. Perlakuan yang di uji adalah ekstrak metanol kulit batang kasturi 25%, 37,5%, 50%, 62,5%, 75%, 87,5% dan 100%. Dan kontrol perlakuan dengan ampisilin dan metanol 70%. Uji bakteri dilakukan menggunakan metode difusi. Parameter yang diukur adalah besaran zona hambat (mm) yang tumbuh pada media MH. Analisis data penelitian mengunakan uji One way ANNOVA dan uji Post Hoc LSD pada α=0,05. Hasil penelitian menunjukan bahwa terdapat perbedaan bermakna diantara perlakuan ekstrak kulit batang kasturi 25%, 50%, 62,5%, 75%, 87,5% dan 100% konsentrasi yang beda di bandingkan dengan ampisilin. Sedangkan pada konsentrasi 37,5% (p<0,05) tidak bermakna. Efektivitas antibakteri yang terbesar di peroleh dari konsentrasi 100%.Kata-kata kunci: kulit batang kasturi, ekstrak metanol, Staphylococcus aureus, zona hambat.

scholarly journals Uji in vitro Aktivitas Antibakteri Minyak Atsiri Batang Sereh (Cymbopogon citratus) terhadap Shigella dysenteriae

2018 ◽  
Vol 6 (2) ◽  
pp. 262
Author(s):  
Yunita Wulansari ◽  
Enny Suswati ◽  
Septa Surya Wahyudi
Keyword(s):  
Antibacterial Activity ◽  
Alternative Therapy ◽  
Tween 80 ◽  
Inhibition Zone ◽  
Negative Control ◽  
Shigella Dysenteriae ◽  
Diffusion Method ◽  
Control Group ◽  
High Morbidity

  Shigella dysenteriae is bacteria that caused diarrhea with high morbidity and mortality. Resistance and toxicity has reported in some antibiotics therapy towards S. dysentriae. Therefore, alternative therapy based on herbal plants is needed. Chymbopogon citratus oil can be used as an antibacterial to Escherichia coli, Klebsiella pneumoniae, Streptococcus mutans, all strains Staphlylococcus sp, Bacillus cereus and Bacillus subtilis. This study aimed to investigate antibacterial activity of C. citratus oils on the growth of S. dysentriae and its minimum inhibitory concentration (MIC). This type of research was a quasi experimental. Antibacterial activity test performed by diffusion method. Subjects in this study were S. dysentriae. C. citratus oil concentration used were 15 μl/ml, 20 μl/ml, 25 μl/ml, 30 μl/ml, 35 μl/ml and 40 μl/ml. Positive control group was given ciprofloxacin 5 μl/ml and a negative control group was given tween-80. Inhibition zone was observed at a concentration of 25 µl/ml (P3). The MIC of C. citratus oils againts S. dysentriae was at a concentration of 25 µl/ml. Kruskal Wallis analysis showed that p=0.000 and α=0.05, it was revealed that there were differences between antibacterial activity amoung tested group with different concentration. Keywords: Shigella dysenteriae, Chymbopogon citratus oil, antibacterial  

scholarly journals Comparison of Antibacterial Effects of Photodynamic Therapy, Modified Triple Antibiotic Paste and Calcium Hydroxide on Root Canals Infected With Enterococcus faecalis: An In Vitro Study

2019 ◽  
Vol 10 (5) ◽  
pp. S23-S29 ◽  
Author(s):  
Mohammad Asnaashari ◽  
Mohammad Jafar Eghbal ◽  
Amirali Sahba Yaghmayi ◽  
Mehdi Shokri ◽  
Saranaz Azari-Marhabi
Keyword(s):  
Photodynamic Therapy ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Control Group ◽  
Root Canals ◽  
Anterior Teeth ◽  
Triple Antibiotic Paste ◽  
Working Length

Introduction: A pivotal issue to achieve success in the treatment of the root canal is root canal disinfection. One of the most important bacteria that infect the root canal is Enterococcus faecalis. This study seeks to examine the effectiveness of 3 methods for disinfecting the root canal: photodynamic therapy, modified triple antibiotic paste (MTAP), and calcium hydroxide. Methods: Sixty-two single-rooted extracted anterior teeth were collected. After cleaning and disinfecting the teeth, their crowns were cut at the CEJ point. The root canals were shaped to the working length up to file F3 ProTaper (F1, F2, F3). EDTA 17% and sodium hypochlorite 2.5% were used for 5 minutes to wash and remove the smear layer, and then the apical foramen was sealed using composite. After that, the teeth were sterilized in an autoclave at 121°C for 15 minutes. Then 10 samples were taken randomly as the negative control. The remaining samples were immersed and cultivated in a suspension containing E. faecalis for 21 days. Then the samples were divided into 5 groups: 2 positive control groups, 1 group treated with the antibiotic paste with the concentration of 1 mg/mL, 1 group treated with calcium hydroxide, and 1 group treated with photodynamic therapy. Then, to collect the biofilm, the ProTaper file F4 was used. After that, the microbial suspension was provided and counting the colonies was carried out to compare the groups. Results: The findings indicated that the amount of CFU/mg of MTAP samples, including clindamycin, metronidazole, ciprofloxacin in the concentration of 1 mg/mL and photodynamic therapy and calcium hydroxide was lower than that in the control group. Antibiotic paste cleansed the root canal up to 99.9%. Photodynamic therapy reduced the amount of CFU/mg to 98.8%, and calcium hydroxide reduced the amount of CFU/mg to 94.13%. Conclusion: Using photodynamic therapy causes a reduction in the biofilm and inhibits the growth of the E. faecalis bacterium. In addition, in this study, MTAP with a concentration of 1 mg/mL was used, which expunged the bacteria completely. Meanwhile, calcium hydroxide had the weakest effect of all on the E. faecalis bacterium.

scholarly journals Lime Peel Liquid (Citrus aurantifolia, Swingle) Inhibit Escherichia Coli In Vitro

2021 ◽  
Vol 4 (1) ◽  
pp. 63
Author(s):  
Devi Indah Sari ◽  
Retno Sri Wahjuni ◽  
Ratih Novita Praja ◽  
Budi Utomo ◽  
Faisal Fikri ◽  
...  
Keyword(s):  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Citrus Aurantifolia ◽  
E Coli ◽  
Randomized Design ◽  
Mueller Hinton ◽  
Lime Peel

The purpose of this study was to determine in vitro inhibition test of lime peel liquid (Citrus aurantifolia, Swingle) against E. coli. The E. coli isolate was cultured on Muller Hinton Agar (MHA) media. Antibacterial test in this study using diffusion method. Negative control (K-) using CMC-Na 0.1%. Positive control (K+) using oxytetracycline antibiotics. The treatments were given lime peel liquid (Citrus aurantifolia, Swingle) with concentrations of 100%, 75%, 50% and 25%, respectively. The study design using Completely Randomized Design (CRD) method, with six treatments and four repetitions. Data were analyzed by Kruskal-Wallis test, Moreover, the Post Hoc Mann-Whitney method. The inhibition zone analysis results were showed that K + had significant differences compared to all treatments K-, 100%, 75%, 50%, and 25%. K- was found to be significantly different from treatments 100%, 75%, 50% and 25%. The results showed that the lime peel liquid has the potential as an antibacterial activity against the growth of E. coli in vitro. Concentration of 25% has shown the presence of inhibitory zones on Mueller Hinton media that have been inoculated with lime peel liquid.

scholarly journals Effectiveness of light-emitting diode exposure on photodynamic therapy against Enterococcus faecalis: in vitro study

2020 ◽  
Vol 53 (2) ◽  
pp. 71
Author(s):  
Nanik Zubaidah ◽  
Agus Subiwahjudi ◽  
Dinda Dewi Artini ◽  
Karina Erda Saninggar
Keyword(s):  
Photodynamic Therapy ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Pathogenic Bacteria ◽  
In Vitro Study ◽  
Control Group ◽  
Root Canal Irrigation ◽  
Group I ◽  
Significant Difference

Background: A successful root canal treatment eliminates pathogenic bacteria from infected root canals. The most common bacteria in root canal infections is Enterococcus faecalis (E. faecalis), due to its resistance to medicament and root canal irrigation. A photodynamic therapy (PDT) is a method of root canal disinfection that uses a combination of photosensitisers and light activation to eliminate bacteria in the root canal. The duration of the PDT irradiation results in the production of singlet oxygen and reactive oxygen species (ROS) to eliminate the E. faecalis bacteria. Purpose: To analyse the differences in the duration exposure of photodynamic therapy against the E. faecalis bacteria. Methods: The E. faecalis bacteria culture was divided into seven eppendorf tubes. Group I was a control group, and group II, III, IV, V, VI and VII were treated using PDT consisting of Toluidine Blue O (TBO) photosensitiser and light source irradiation for ten, 20, 30, 40, 50 and 60 seconds, respectively. After incubation, the number of bacteria was calculated by the Quebec Colony Counter and analysed using the Kruskal–Wallis test and the Mann–Whitney test (p <0.05). Results: There was a significant difference between the number of E. faecalis bacteria colonies in each treatment group (p <0.05). Group VI and VII, which had a longer exposure to PDT, showed a smaller amount of E. faecalis bacteria. Conclusion: The longer exposure of PDT results in a smaller amount of E. faecalis bacteria. The light irradiation of 50 seconds is the most effective to eliminate E. faecalis bacteria.

scholarly journals The Difference of Antibacterial Power Between Cocoa Peel (Theobroma cacao) Extract 6% Compared to NaOCL 5% Againts Lactobacillus acidophilus

2021 ◽  
Vol 11 (2) ◽  
pp. 67
Author(s):  
Tamara Yuanita ◽  
Dian Agustin Wahjuningrum ◽  
Mega Selvia
Keyword(s):  
Lactobacillus Acidophilus ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Control Group ◽  
Control Group Design ◽  
Post Test ◽  
The Difference ◽  
Peel Extract

Background: Lactobacillus acidophilus is a gram-positive rod-shaped bacteria which mostly causes chronic caries lesions. In the process of cavity preparation it is reported that it is not possible to remove all microorganisms and even bacteria can survive even after removal of the necrotic tissue. Efforts to stop the growth of these bacteria are by chemical cavity cleanser using a 5% NaOCl, but this material have disadvantages including irritating periradicular tissue, unpleasant odor, and toxicity. Due to its disadvantages, it is hoped that natural ingredients can be used as alternatives. Cocoa peel extract has active compounds of tannins, flavonoids, alkaloids, terpenoids, and saponins which have antibacterial ability with a concentration of 6% according to the non-toxic minimum kill concentration of Lactobacillus acidophilus. Purpose: To analyze the difference in antibacterial power between the extract of cocoa bark with a concentration of 6% compared to 5% NaOCl against Lactobacillus acidophilus. Methods: This study was a laboratory experimental in vitro with a post test-only control group design. Using diffusion method against Lactobacillus acidophilus grown in tubes containing BHIB, then cultured in a petridish which contains a nutrient agar and is divided into 2 parts extract cocoa peel extract 6% and NaOCl 5%, then each petridish given a paper disc that has been poured 10μl by each material, then incubated inthe incubator for 2x24 hours at 37oC and observe the diameter of the inhibition zone formed using a caliper. Results: The average diameter of the inhibition zone formed in the 6% cocoa peel extract was 11.8375 mm and NaOCl 5% was 26.0344 mm against Lactobacillus acidophilus. Conclusion: There is a difference in antibacterial power between 6% cocoa peel extract and 5% NaOCl against Lactobacillus acidophilus.

scholarly journals Antibakteri Ekstrak Kulit Buah Kakao (Theobroma cacao) 6,25% dan NaOCl 2,5% Terhadap Bakteri Streptococcus sanguinis

2019 ◽  
Vol 9 (1) ◽  
pp. 19
Author(s):  
Sepdhyo Wahyu Nugroho ◽  
Mandojo Rukmo ◽  
Edhie Arief Prasetyo ◽  
Tamara Yuanita
Keyword(s):  
Theobroma Cacao ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Control Group ◽  
Control Group Design ◽  
Streptococcus Sanguinis ◽  
Petri Dishes ◽  
Peel Extract

Background: Streptococcus sanguinis is a gram-positive bacterium that infects and penetrates into dentinal tubules from a depth of 150 μm up to 792 μm. Chemicals, namely 2.5% NaOCl, are used to mitigate the growth of these bacteria via irrigation of the root canals, but 2.5% NaOCl still has disadvantages including irritating periradicular tissue, having an unpleasant odor, and being toxic. Due to these shortcomings, natural materials are expected to be used as alternatives. Cocoa peel extract has active tannin compounds, flavonoids, alkaloids, terpenoids, and saponins which have antibacterial attributes; a concentration of 6.25% is counted as the minimum bactericidal concentration (MBC) of Streptococcus sanguinis. Aim: To compare the difference of antibacterial power of 6.25% cocoa peel extract (Theobroma cacao) and 2.5% NaOCl against Streptococcus sanguinis. Method: This research is an in vitro experimental laboratory with post-test only control group design. The diffusion method was used on Streptococcus sanguinis planted in tubes containing BHIB, then cultured on petri dishes containing nutrient agar and divided into 3 parts, namely 6.25% cocoa peel extract, 2.5% NaOCl, and negative controls, then each Petri dishes were given a paper disc that had been dripped by 0.01 ml of each ingredient, then incubated in an incubator for 2x24 hours at 37°C; the diameter of the inhibitory zone formed was then observed using a caliper. Results: The average diameter of the inhibition zone formed on 6.25% cocoa peel extract was 19.2000 mm and 2.5% NaOCl was 17.2813 mm against Streptococcus sanguinis. Conclusion: The antibacterial power of 6.25% cocoa peel extract (Theobroma cacao) is higher than 2.5% NaOCl against Streptococcus sanguinis.

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