scholarly journals STANDARDIZATION OF HYDROPONIC AND WILD GROWING TEUCRIUM POLIUM L. BY HPLC

Author(s):  
Galstyan H. M ◽  
Dumanyan K. H ◽  
Tsaturyan A. O ◽  
Gukasyan N. H ◽  
Engibaryan A. A ◽  
...  

It was approved that flavonoids (luteoline, apigenin) and phenylpropanoid glycosides verbascoside best separation was implemented by using UM detector and isocratic isolation regime. Depending on the growing conditions main influencing substance quantities fluctuate: in the wild growing plants were synthesized nearly 2 times more than in hydroponic growing plants. For the study were obtained 500 alcoholic extracts from hydroponic and wild growing T. polium. The studied samples before analysis were developed three-chloral-acetic-acid anhydride and centrifuged by 10 minutes 12000 rev/m speeds. The solution volume of each injected analysis was 10 mkl. Then some amount of standard verbascoside, luteolin and apigenin samples were solved in 1ml ethyl alcohol and were filled into the special test tubes for analysis. By T. polium chloroform-methanol and tower chromatography water fractions teupolizoid, verbascoside and poliumozid phenylpropanoid glycosides were separated which Rf were equal to 0.25, 0.5, 0.37 in our researches in the past. These compounds composition was proved by complex spectroscopic methods. They had close structure to each other and verbascoside enclosed peaks in HPLC were supposed that belong to poliumozid and teupoliozid and also was brought the above mentioned compounds quantities by calculating chromatography data. Keywords: Teucrium polium L., wild and hydroponic, HPLC, standardization, verbascoside, apigenine, luteolin.

2016 ◽  
Vol 42 (2) ◽  
pp. 21-39
Author(s):  
Nadjia Fertout-Mouri ◽  
Ali Latrèche ◽  
Zoheir Mehdadi ◽  
Soror Akli-Djaaboub ◽  
Adel Akli

Author(s):  
Brijesh Charadva ◽  
Urvashi Ghataliya ◽  
Pooja Meena ◽  
Krishna Karia ◽  
Tixa Lakhlani ◽  
...  

Launaea procumbens leaves are galactagogue, diuretic, antifungal, anorexic, anti-arthritic and hepatoprotective according to Ayurvedic texts as well as modern research. Launaea species, particularly L. pinnatifida, is mentioned as one of the sources of Gojihva, regarding which there is controversy with respect to its true botanical identity. However, no detailed anatomical, phytochemical or chromatographic investigation is available for leaf of L. procumbens which can differentiate it from L. pinnatifida. The aim of this work is to develop standardization parameters of L. procumbens leaves by performing its pharmacognostical evaluation, preliminary phytochemical screening, HPTLC and GC-MS fingerprints. Pharmacognostic investigation of the leaves was performed by its morphological study, qualitative and quantitative microscopy as well as powder microscopy. Extraction of leaves was done by maceration using methanol. This extract was used for preliminary phytochemical screening and chemoprofiling by GC-MS, as well as for developing its HPTLC fingerprint. A mobile phase system was developed by pilot TLC, following which an HPTLC fingerprint was performed using the solvent system chloroform: methanol: ethyl acetate (3:7:6). Diagnostic microscopic characters identified in powder include unicellular covering trichome, xylem vessels, anomocytic stomata, and prisms of calcium oxalate crystal. Phytochemical screening revealed the presence of phytoconstituents classes like phenolics, anthraquinones, saponin glycosides, carbohydrates, sterols, triterpenoids and flavonoids. HPTLC fingerprinting detected 6 peaks with Rf 0.25, 0.37, 0.41, 0.50, 0.65, 0.78 at 254nm and 4 peaks with Rf 0.32, 0.37, 0.50, 0.66 at 366nm. GC-MS fingerprint revealed presence of propanoic acid anhydride, valeric anhydride, 2- Pyrrolidine acetic acid, phthalan, 5- (Hydroxy methyl)-2-(dimethoxy methyl) furan, vanillin, methyl β-l-Arabino pyranoside, 1,6-anhydro-β-D-Gluco pyranose and 6-Methyl-2-Heptanone, 6-(3,5-dimethyl-2-furanyl). Present study will be very useful for herbal industry in differentiating Launaea procumbens from other species of the genus Launaea, particularly L. pinnatifida (Gojihva) as well as for authentication, standardization and detection of adulteration in the leaf formulations of Launaea procumbens.


1991 ◽  
Vol 27 (5) ◽  
pp. 556-559 ◽  
Author(s):  
G. B. Oganesyan ◽  
A. M. Galstyan ◽  
V. A. Mnatsakanyan ◽  
A. S. Shashkov ◽  
P. V. Agababyan

Author(s):  
Derya Soydaner

In recent years, we have witnessed the rise of deep learning. Deep neural networks have proved their success in many areas. However, the optimization of these networks has become more difficult as neural networks going deeper and datasets becoming bigger. Therefore, more advanced optimization algorithms have been proposed over the past years. In this study, widely used optimization algorithms for deep learning are examined in detail. To this end, these algorithms called adaptive gradient methods are implemented for both supervised and unsupervised tasks. The behavior of the algorithms during training and results on four image datasets, namely, MNIST, CIFAR-10, Kaggle Flowers and Labeled Faces in the Wild are compared by pointing out their differences against basic optimization algorithms.


Author(s):  
Shakhnoza S. Azimova ◽  
Anna I. Glushenkova

Zootaxa ◽  
2011 ◽  
Vol 2734 (1) ◽  
pp. 23 ◽  
Author(s):  
STEPHEN MAHONY

Two new species of the genus Megophrys are described from historical collections presented by Malcolm A. Smith to The Natural History Museum, London, in the early twentieth century. These specimens were previously misidentified as Megophrys parva, a widespread Asian species apparently comprised of a species complex. Megophrys damrei sp. nov., from the Bokor Plateau in the Cardamom Mountains of southern Cambodia and Megophrys takensis sp. nov., from Ban Pa Che, Tak Province, in western Thailand, are herein distinguished morphologically from all congeners from their respective and neighbouring countries. These, or further specimens representing either species, do not appear to have been reported on by other authors in the past and the conservation status of the two new species remains to be assessed. An attempt to locate both species at and near their respective type localities was successful for Megophrys takensis sp. nov., allowing the documentation of observations in the wild and live colouration. This study highlights the importance of examining historical museum collections often overlooked in recent decades by modern taxonomists.


Phytomedicine ◽  
2004 ◽  
Vol 11 (1) ◽  
pp. 83-84 ◽  
Author(s):  
E. Mazokopakis ◽  
S. Lazaridou ◽  
M. Tzardi ◽  
J. Mixaki ◽  
I. Diamantis ◽  
...  

1998 ◽  
Vol 24 ◽  
pp. 31-39
Author(s):  
M. Dattilo

SummaryThe Giara pony is a less known, equine breed of small stature, typical of the island of Sardinia. It draws its denomination from the highland of the Giara, where it lives in the wild.The ponies appeared in Sardinia approximately in the VII century B. C. Recent research, carried out on haemoglobin polymorphism, underlined some analogies with the Arabic horses of North-Africa.These ponies, have a dolyichomorphic-type constitution with long, naked and resistant legs. In the past they were used for agricultural work, however later, with the increase of mechanisation, they returned to the Giara highland where they became feral.The Giara ponies, in the light of recent scientific research, possess not only an inestimable genetic patrimony but they also represent a large source of interest for the equestrian sport, and, above all, for children's riding therapy.The author underlines, finally, that the principal material of this article has been outlined in a symposium held at the University of Sassari 18/11/1997, entitled: “Phenomenology of the environment: psycho-social and zoo-anthropological perspectives”.


1999 ◽  
Vol 4 (2) ◽  
pp. 51 ◽  
Author(s):  
Hellen Kurniati ◽  
Yunus Rumbarar ◽  
Mumpuni Mumpuni ◽  
Gono Semiadi

The people of Indonesia have historically used crocodiles for a variety of non-commercial purposes. In the past ten years, a commercial collection from the wild almost threatened the population status. As pressure on the commercial hunting continuous, the successful conservation of Indonesian crocodiles will depend on the constant monitoring in the wild. Irian Jaya is one of several islands in Indonesia which historically accommodate an unknown number of commercial crocodiles such as Crocolylus porosus. Althorough monitoring on the C. porosus status in Barusam Gasawi, Buruai Garawa, Kamabu rivers, and Suwiki lake located in Kaimana and Arguni Bay, Irian Jaya had been conducted since 1990 and continued in 1991, 1992, 1993, 1994, 1996, and 1998. The surveys techniques were conducted night count method and guided by a 1: 100.000 map and GPS. A statistical analysis was performed on SAS using proc regression and proc mean. A significant result has only been noted in Suwiki Lake, where was the magnitude of the mean trend in density was 109 percent.


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