scholarly journals Promotion of extrinsic apoptosis pathway in HCT-116 human colorectal cancer cell line by sodium butyrate as histone deacetylase inhibitor

2020 ◽  
Keyword(s):  
Gene Expression ◽  
Colorectal Cancer ◽  
Cell Line ◽  
Real Time ◽  
Histone Deacetylase ◽  
Sodium Butyrate ◽  
Histone Deacetylase Inhibitor ◽  
Control Group ◽  
Deacetylase Inhibitor ◽  
Hct 116

Background: Colorectal cancer (CRC) has already been considered the fourth leading cause of mortality worldwide as the genes involved in apoptotic pathways and alterations of reversible epigenetic have an important role in the progression of CRC. Objectives: The current study aimed to evaluate the effect of sodium butyrate as a histone deacetylase inhibitor on the alterations of the gene expression of FAS, Fas ligand (FASL), Death receptor 4 in HCT-116 CRC cell line. Methods: HCT-116 cell line was cultured in Dulbeccoʼs Modified Eagle Medium. The cytotoxicity effect of sodium butyrate on HCT-116 was evaluated using 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyltetrazolium bromide assay for three incubation times (i.e., 24, 48, and 72 h). The half-maximal inhibitory concentration (IC50) values were determined. The optimum concentration was within the range of 6.25-200 mM. The cellular ribonucleic acid was extracted, and complementary deoxyribonucleic acid was synthesized. Finally, the alterations of the gene expression of FAS, FASL, DR4, DR5, and TRAIL were assessed by real-time polymerase chain reaction (PCR). Results: The IC50 levels for three incubation times were 50, 12.5, and 6.25 mM, respectively. The obtained results of real-time PCR demonstrated a significant increase in the gene expression of TRAIL, DR5, and FAS in comparison to that of the untreated cells as the control group at the three incubation times. The DR4 gene expression significantly increased in comparison to that reported for the control group at 48 and 72 h of incubation. In addition, FASL gene expression remarkably decreased at the three incubation times. Conclusions: Sodium butyrate could show cytotoxicity effect on CRC cell lines through the induction of death receptors in the extrinsic apoptotic pathway. The obtained results of this study revealed that the optimum effect of sodium butyrate is an incubation time-dependent and concentration-dependent manner.

57 GENE EXPRESSION PATTERN OF MINIATURE PIG SOMATIC CELL NUCLEAR TRANSFER EMBRYOS TREATED WITH THE HISTONE DEACETYLASE INHIBITOR SCRIPTAID

2011 ◽  
Vol 23 (1) ◽  
pp. 134
Author(s):  
C. H. Park ◽  
S. G. Lee ◽  
H. J. Lee ◽  
T. K. Jung ◽  
Y. H. Jeong ◽  
...  
Keyword(s):  
Gene Expression ◽  
Histone Deacetylase ◽  
Expression Pattern ◽  
Histone Deacetylase Inhibitor ◽  
Gene Expression Pattern ◽  
Control Group ◽  
Developmental Potential ◽  
Deacetylase Inhibitor

It was recently shown that treatment of cloned embryos with histone deacetylase inhibitors improves efficiency for the success rate of developmental potential to term in several species. The objective of the present study was to investigate the influence of the histone deacetylase inhibitor Scriptaid (Sc) on in vitro development in early porcine SCNT embryos and on their gene expression pattern. Based on the findings of previous porcine studies (Zhao et al. 2009), the reconstructed oocytes were treated with 500 nM Scriptaid for 14 to 16 h after post-fusion activation (6-DMAP/demecolcine). In our preliminary study, blastocyst rate significantly increased in the Sc-treated group, compared with the control group (25.1 ± 2.8% and 13.8 ± 1.9%, respectively, P < 0.05). We determined gene expression using quantitative real-time RT-PCR. The results showed that OCT3/4 gene was expressed at a similar level in in vivo and SCNT blastocysts with/without Sc. IGF2 and H19 genes tended to be highly expressed in both SCNT blastocysts with (1.6-fold and 3.1-fold, respectively) and without (2.0-fold and 5.8-fold, respectively) Sc than that of the in vivo blastocysts. We found differences in imprinted gene expression patterns between in vivo and cloned blastocysts. Expression of H19 and IGF2 in SCNT blastocysts after Scriptaid treatment decreased towards the expression levels of in vivo blastocysts. These results indicated that Scriptaid treatment in SCNT embryos may also have beneficial effects on in vitro developmental competence as well as their gene expression pattern.

scholarly journals The Histone Deacetylase Inhibitor AN7, Attenuates Choroidal Neovascularization in a Mouse Model

2019 ◽  
Vol 20 (3) ◽  
pp. 714
Author(s):  
Mor Dahbash ◽  
Ruti Sella ◽  
Elinor Megiddo-Barnir ◽  
Yael Nisgav ◽  
Nataly Tarasenko ◽  
...  
Keyword(s):  
Growth Factor ◽  
Mouse Model ◽  
Histone Deacetylase ◽  
Choroidal Neovascularization ◽  
Histone Deacetylase Inhibitor ◽  
Vision Loss ◽  
Age Related Macular Degeneration ◽  
Control Group ◽  
Age Related ◽  
Deacetylase Inhibitor

: Choroidal neovascularization (CNV) is a complication of age-related macular degeneration and a major contributing factor to vision loss. In this paper, we show that in a mouse model of laser-induced CNV, systemic administration of Butyroyloxymethyl-diethyl phosphate (AN7), a histone deacetylase inhibitor (HDACi), significantly reduced CNV area and vascular leakage, as measured by choroidal flatmounts and fluorescein angiography. CNV area reduction by systemic AN7 treatment was similar to that achieved by intravitreal bevacizumab treatment. The expression of vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF-2), and the endothelial cells marker CD31, was lower in the AN7 treated group in comparison to the control group at the laser lesion site. In vitro, AN7 facilitated retinal pigmented epithelium (RPE) cells tight junctions’ integrity during hypoxia, by protecting the hexagonal pattern of ZO-1 protein in the cell borders, hence reducing RPE permeability. In conclusion, systemic AN7 should be further investigated as a possible effective treatment for CNV.

Sign in / Sign up

Export Citation Format

Share Document