scholarly journals Konsentrasi Didecyl Dimethyl Ammonium Chloride Sebagai Antimikroba Terhadap Isolat Bakteri Secara In Vitro

2018 ◽  
Vol 4 (2) ◽  
Author(s):  
Atira Atira ◽  
Iim Sunti ◽  
Yuni Rizki Amalia

Latar Belakang: Didecyl Dimethyl Ammonium Chloride merupakan salah satu jenis desinfektan yang mengandung senyawa antimikroba. Penelitian ini bertujuan untuk mengetahui konsentrasi hambat minimal  antimikroba Didecyl Dimethyl Ammonium Chloride terhadap isolat bakteri dari ruang perawatan RS Al-Ihsan Bandung. Metode: metode yang digunakan dalam penelitian ini yaitu Tru Experiment dengan menggunakan desain rancangan acak lengkap. Sampel penelitian terdiri atas 6 kelompok perlakuan yang masing-masing dilakukan 4 kali pengulangan. Data dianalisis secara statistik dengan menggunakan Anova  satu arah dengan uji lanjut menggunakan analisis Duncan (α=0,05). Penelitian ini dilaksanakan di Laboratorium Hayati Institut Teknologi Bandung. Hasil: hasil penelitian menunjukkan bahwa Didecyl Dimethyl Ammonium Chloride pada berbagai konsentrasi 50%, 25%, 12,5%, 6,25%, 3,12%, dan 0,00 (kontrol) memiliki daya antimikroba terhadap isolat bakteri konsentrasi 106/mL secara in vitro dengan metode sumuran modifikasi Kirby-Bauer pada cawan petri yang berisi medium agar nutrien. Uji statistik menunjukkan pengaruh yang signifikan pada daya hambat dengan penggunaan konsentrasi Didecyl Dimethyl Ammonium Chloride yang berbeda. Hasil uji lanjut analisis Duncan menunjukkan beda rata-rata yang bermakna untuk perlakuan konsentrasi hambat minimal Didecyl Dimethyl Ammonium Chloride 3,12% memberikan daya hambat terhadap pertumbuhan bakteri rata-rata 0,378 cm (3,78 mm) dengan nilai signifikansi sebesar 0,000. Kesimpulan: konsentrasi  hambat minimal Didecyl Dimethyl Ammonium Chloride sebagai antibakteri yaitu 3,12%. 

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Guo Xiling ◽  
Chen Yin ◽  
Wang Ling ◽  
Wu Xiaosong ◽  
Fan Jingjing ◽  
...  

AbstractSevere acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection is currently a global pandemic, and there are limited laboratory studies targeting pathogen resistance. This study aimed to investigate the effect of selected disinfection products and methods on the inactivation of SARS-CoV-2 in the laboratory. We used quantitative suspension testing to evaluate the effectiveness of the disinfectant/method. Available chlorine of 250 mg/L, 500 mg/L, and 1000 mg/L required 20 min, 5 min, and 0.5 min to inactivate SARS-CoV-2, respectively. A 600-fold dilution of 17% concentration of di-N-decyl dimethyl ammonium bromide (283 mg/L) and the same concentration of di-N-decyl dimethyl ammonium chloride required only 0.5 min to inactivate the virus efficiently. At 30% concentration for 1 min and 40% and above for 0.5 min, ethanol could efficiently inactivate SARS-CoV-2. Heat takes approximately 30 min at 56 °C, 10 min above 70 °C, or 5 min above 90 °C to inactivate the virus. The chlorinated disinfectants, Di-N-decyl dimethyl ammonium bromide/chloride, ethanol, and heat could effectively inactivate SARS-CoV-2 in the laboratory test. The response of SARS-CoV-2 to disinfectants is very similar to that of SARS-CoV.


2001 ◽  
Vol 66 (9) ◽  
pp. 631-636
Author(s):  
Rangel Igov ◽  
Violeta Mitic ◽  
Todor Pecev ◽  
Vesna Stankov-Jovanovic

A new reaction is suggested and a new method is elaborated for determination of micro amounts of Sn(II) based on its inhibiting effect on the oxidation of {4-bis[n-(dimethylamino) phenyl]methylene-2,5-cyclohexadiene-1-ilydene}dimethyl-ammonium chloride (crystal violet CV) by H2O2. The method sensibility is 0.4 ?g/cm3. The probable relative error is 2.8-12.8 % for Sn(II) in the concentration interval of 3 to 0.8 ?g/cm3. The kinetic equation for this process is given. The influence of some other ions on the reaction rate was tested. The method was applied to the determination of Sn(II) in a sample of microalloy.


2020 ◽  
Vol 44 (47) ◽  
pp. 20520-20524
Author(s):  
Yuxue Dai ◽  
Peng Gao ◽  
Xueying Wang ◽  
Pengfei Wang ◽  
Dandan Gao ◽  
...  

CoP layer was successfully deposited onto poly(diallyl dimethyl ammonium chloride) (PDDA) functionalized carbon nanotubes (CoP/PDDA@CNTs) as high effective OER electrocatalysts in alkaline solution.


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