Treatment of postoperative wounds using pectin-based reinforced dressings and their antimicrobial activity

A reinforced pectin-based dressing with a reinforcing element containing the antimicrobial agent chlorhexidine bigluconate has been developed. In vitro studies have shown that the hydrogel pectin dressing containing 0.03 ÷ 1.5 % chlorhexidine bigluconate inhibits the growth of both gram-positive (S. aureus) and gram-negative (P. aeruginosa) bacteria. The dressing can be used in the complex treatment of postoperative wounds with infectious-inflammatory process. The efficacy of different doses of chlorhexidine bigluconate was characterized by growth inhibition and increase of microorganism-free areas on the culture medium around the site of dressing localization, and regardless of the type of bacteria. Bacterial growth inhibition radius size depends on the dose of chlorhexidine in the hydrogel pectin dressing. The inhibition of growth of S. aureus and P. aeruginosa is directly proportional to chlorhexidine bigluconate content. The increase of dressing saturation with chlorhexidine to 1.0 and 1.5 % recorded the maximum inhibition of the growth of microorganisms. A veterinary clinical trial has shown a good therapeutic effect in the wound healing, in particular in the complex treatment of postoperative and accidental wounds both in the presence of infectious-inflammatory process and in its absence. The reinforced pectin-based dressing with cotton (or polypropylene) reinforcement element containing chlorhexidine bigluconate reduces the cost of dressings and bandaging frequency during wound healing. It protects the wound surface from contamination, mechanical irritation, bacterial contamination and the development of secondary infection. The dressing promotes good water, air and heat exchange between the wound and the environment, adsorbs excess exudate, maintains a moist environment and does not cause hyperosmotic damage and drying of the wound. Surgical wound healing occurred under the initial tension for 7 days. Considering the method of its application, this dressing is suitable for use on different parts of the animal's body (neck, withers, chest and abdomen, lower back, buttocks, thighs, shoulders, etc.).

Download Full-text

In Vivo and In Vitro Studies of Antibiotic Release from and Bacterial Growth Inhibition by Antibiotic-Impregnated Polymethylmethacrylate Hip Spacers

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.50.1.332-335.2006 ◽

2006 ◽

Vol 50 (1) ◽

pp. 332-335 ◽

Cited By ~ 40

Author(s):

Jens Kelm ◽

Thilo Regitz ◽

Eduard Schmitt ◽

Werner Jung ◽

Konstantinos Anagnostakos

Keyword(s):

Wound Healing ◽

Growth Inhibition ◽

Bacterial Growth ◽

Antimicrobial Properties ◽

In Vitro Studies ◽

Antibiotic Release ◽

Bacterial Growth Inhibition

ABSTRACT The antimicrobial properties and the elution characteristics of gentamicin-vancomycin-loaded hip spacers were studied in vivo and in vitro. Vancomycin elution was greater than gentamicin elution. The antibiotic concentrations in vivo were less than those in vitro. Not dependent on implantation duration, growth inhibition by spacers in vitro was observed for 2 weeks. The reason for protracted wound healing cannot be insufficient antibiotic release.

Download Full-text

Microbial Growth Inhibition by Alternating Electric Fields in Mice with Pseudomonas aeruginosa Lung Infection

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01841-09 ◽

2010 ◽

Vol 54 (8) ◽

pp. 3212-3218 ◽

Cited By ~ 27

Author(s):

Moshe Giladi ◽

Yaara Porat ◽

Alexandra Blatt ◽

Esther Shmueli ◽

Yoram Wasserman ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Growth Inhibition ◽

Electric Fields ◽

Bacterial Growth ◽

Lung Infection ◽

Alternating Electric Fields ◽

Microbial Growth Inhibition ◽

Bacterial Growth Inhibition

ABSTRACT High-frequency, low-intensity electric fields generated by insulated electrodes have previously been shown to inhibit bacterial growth in vitro. In the present study, we tested the effect of these antimicrobial fields (AMFields) on the development of lung infection caused by Pseudomonas aeruginosa in mice. We demonstrate that AMFields (10 MHz) significantly inhibit bacterial growth in vivo, both as a stand-alone treatment and in combination with ceftazidime. In addition, we show that peripheral (skin) heating of about 2°C can contribute to bacterial growth inhibition in the lungs of mice. We suggest that the combination of alternating electric fields, together with the heat produced during their application, may serve as a novel antibacterial treatment modality.

Download Full-text

In Vitro Antimicrobial Activity of an Experimental Dentifrice Based on Ricinus Communis

Brazilian Dental Journal ◽

10.1590/0103-6440201302382 ◽

2014 ◽

Vol 25 (3) ◽

pp. 191-196 ◽

Cited By ~ 12

Author(s):

Vanessa Maria Fagundes Leite ◽

Juliana Barchelli Pinheiro ◽

Marina Xavier Pisani ◽

Evandro Watanabe ◽

Raphael Freitas de Souza ◽

...

Keyword(s):

Antimicrobial Activity ◽

Growth Inhibition ◽

Bacterial Growth ◽

Ricinus Communis ◽

Candida Spp ◽

E Coli ◽

Reflected Light ◽

Bacterial Growth Inhibition ◽

Post Hoc

This study evaluated the antimicrobial activity of a Ricinus communis-based experimental dentifrice for denture hygiene against the following standard strains: Staphylococcus aureus, Escherichia coli, Streptococcus mutans, Enterococcus faecalis, Candida albicans and Candida glabrata. The minimum inhibitory concentration (MIC) assay was performed with R. communis in pure oil at 2.5%. Only E. coli was not inhibited by R. communis, but the MIC (0.0781%) was effective against the other microorganisms. From these results it was determined the R. communis concentrations for experimental dentifrices, 1, 2, 5 and 10%, which were evaluated by the test-well diffusion in agar. The commercial dentifrices Colgate, Trihydral and Corega Brite were tested for comparative purposes. The diameter of the zones of bacterial growth inhibition produced around the wells was measured (in mm) with a rule under reflected light. Data were analyzed statistically by analysis of variance and Tukey's post-hoc test (α=0.05). Neither the commercial nor the experimental dentifrices were effective against E. coli. The experimental dentifrices containing R. communis at 2, 5 and 10% presented action against S. mutans, S. aureaus and E. faecallis. The experimental dentifrices showed no antimicrobial activity against Candida spp. and E. coli in any of the tested concentrations. Trihydral was the most effective. Comparing the experimental dentifrices, the product with 10% R. communis produced the largest zones of bacterial growth inhibition and had similar antimicrobial activity to the commercial dentifrices, except against S. aureus.

Download Full-text

Ibuprofen-Loaded Heparin Modified Thermosensitive Hydrogel for Inhibiting Excessive Inflammation and Promoting Wound Healing

Polymers ◽

10.3390/polym12112619 ◽

2020 ◽

Vol 12 (11) ◽

pp. 2619

Author(s):

Abegaz Tizazu Andrgie ◽

Haile Fentahun Darge ◽

Tefera Worku Mekonnen ◽

Yihenew Simegniew Birhan ◽

Endiries Yibru Hanurry ◽

...

Keyword(s):

Wound Healing ◽

Drug Carrier ◽

Saline Group ◽

Mice Model ◽

Thermosensitive Hydrogel ◽

Vitro Assay ◽

Raw264.7 Macrophages ◽

Moist Environment

Hydrogels have been investigated as ideal biomaterials for wound treatment owing to their ability to form a highly moist environment which accelerates cell migration and tissue regeneration for prompt wound healing. They can also be used as a drug carrier for local delivery, and are able to activate immune cells to enhance wound healing. Here, we developed heparin-conjugated poly(N-isopropylacrylamide), an injectable, in situ gel-forming polymer, and evaluated its use in wound healing. Ibuprofen was encapsulated into the hydrogel to help reduce pain and excessive inflammation during healing. In addition to in vitro studies, a BALB/c mice model was used to evaluate its effect on would healing and the secretion of inflammatory mediators. The in vitro assay confirmed that the ibuprofen released from the hydrogel dramatically reduced lipopolysaccharide-induced inflammation by suppressing the production of NO, PGE2 and TNF-α in RAW264.7 macrophages. Moreover, an in vivo wound healing assay was conducted by applying hydrogels to wounds on the backs of mice. The results showed that the ibuprofen-loaded hydrogel improved healing relative to the phosphate buffered saline group. This study indicates that ibuprofen loaded in an injectable hydrogel is a promising candidate for wound healing therapy.

Download Full-text

In vitro studies of water activity and bacterial growth inhibition of sucrose-polyethylene glycol 400-hydrogen peroxide and xylose-polyethylene glycol 400-hydrogen peroxide pastes used to treat infected wounds.

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.35.9.1799 ◽

1991 ◽

Vol 35 (9) ◽

pp. 1799-1803 ◽

Cited By ~ 9

Author(s):

U Ambrose ◽

K Middleton ◽

D Seal

Keyword(s):

Hydrogen Peroxide ◽

Polyethylene Glycol ◽

Growth Inhibition ◽

Water Activity ◽

Bacterial Growth ◽

In Vitro Studies ◽

Polyethylene Glycol 400 ◽

Infected Wounds ◽

Bacterial Growth Inhibition

Download Full-text

Efficacy of three hygienic protocols in reducing biofilm adherence to removable thermoplastic appliance

The Angle Orthodontist ◽

10.2319/012413-75.1 ◽

2013 ◽

Vol 84 (1) ◽

pp. 161-170 ◽

Cited By ~ 9

Author(s):

Nir Shpack ◽

Ronit Bar-Ness Greenstein ◽

Dana Gazit ◽

Rachel Sarig ◽

Alexander Dan Vardimon

Keyword(s):

Growth Inhibition ◽

Bacterial Growth ◽

Gentian Violet ◽

Bacterial Biofilm ◽

Incisal Edge ◽

Cleaning Solution ◽

Bacterial Growth Inhibition

ABSTRACT Objectives: To examine the ability of a removable thermoplastic appliance (RTA) to adsorb hygienic solutions and inhibit bacterial growth and to examine the efficacy of three hygiene protocols in reducing bacterial biofilm adherence to RTA. Materials and Methods: Solution adsorption and bacterial growth inhibition were examined in vitro using paper vs RTA discs. Subsequently, 11 patients treated with RTA (mean age, 29.1 ± 4.7 years) were assigned into a sequence of three hygiene protocols: regular RTA brushing (baseline), immersion RTAs in chlorhexidine mouthwash (CHX), and using a vibrating bath with cleaning solution (VBC). For each patient, 12 upper RTAs were examined (2 baseline RTAs, 5 CHX RTAs, and 5 VBC RTAs), for a total of 132 RTAs. All RTAs were stained with gentian violet, and biofilm presence was measured using a photodensitometer. Results: The RTA discs did not adsorb CHX or cleaning solution. The later agent did not show antibacterial features. Baseline RTAs showed significant biofilm adherence (P < .001) on the posterior palatal side of the aligner and on the anterior incisal edge. CHX and VBC hygienic protocols significantly (P < .001) reduced baseline biofilm adherence by 16% and 50%, respectively. Hygienic improvement was maintained over 140 days when CHX and VBC were used. However, VBC was three times more efficient than CHX. Conclusions: This study highly recommends the use of a VBC protocol. Biofilm deposits on the RTA, especially on incisal edges and attachment dimples, could lead to inadequate tooth/RTA and attachment/RTA overlap and consequently impair tooth alignment.

Download Full-text

In vitro adrenalin oxidation, in bacteriological media, causes bacterial growth inhibition and killing

FEMS Microbiology Letters ◽

10.1111/j.1574-6968.1990.tb13829.x ◽

1990 ◽

Vol 67 (1-2) ◽

pp. 21-26 ◽

Cited By ~ 2

Author(s):

Sergio Giunta ◽

Luciano Galeazzi ◽

Gianni Turchetti ◽

Giordano Grilli ◽

Giuseppe Groppa

Keyword(s):

Growth Inhibition ◽

Bacterial Growth ◽

Bacterial Growth Inhibition

Download Full-text

In vitro study of bacterial growth inhibition in concentrated sugar solutions: microbiological basis for the use of sugar in treating infected wounds.

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.23.5.766 ◽

1983 ◽

Vol 23 (5) ◽

pp. 766-773 ◽

Cited By ~ 77

Author(s):

J Chirife ◽

L Herszage ◽

A Joseph ◽

E S Kohn

Keyword(s):

Growth Inhibition ◽

Bacterial Growth ◽

In Vitro Study ◽

Vitro Study ◽

Infected Wounds ◽

Bacterial Growth Inhibition

Download Full-text

Susceptibility of cariogenic microorganisms to phytoconstituents

Brazilian Journal of Biology ◽

10.1590/1519-6984.174147 ◽

2018 ◽

Vol 78 (4) ◽

pp. 691-696 ◽

Cited By ~ 2

Author(s):

G. L. S. Ferreira ◽

L. M. D. Bezerra ◽

I. L. A. Ribeiro ◽

R. C. D. Morais Júnior ◽

R. D. Castro

Keyword(s):

Antibacterial Activity ◽

Growth Inhibition ◽

Brain Heart Infusion ◽

Positive Control ◽

Tetrazolium Chloride ◽

Streptococcus Oralis ◽

Potential Applications ◽

In Vitro Antibacterial Activity ◽

Bacterial Growth Inhibition

Abstract This study aimed to evaluate the in vitro antibacterial activity of the phytochemicals thymol, linalool, and citronellol against Streptococcus mutans, Streptococcus salivarius and Streptococcus oralis. Disk diffusion screening on solid medium and measurement of the diameter of the bacterial growth inhibition halos was the technique utilized. The Minimum Inhibitory Concentration (MIC) of the substances was determined using serial substance dilutions and microdilution technique in Brain Heart Infusion culture medium. After incubation for 24 hours in an oven at 37 °C, plate reading was completed and confirmed by visual method using 2,3,5 triphenyl tetrazolium chloride dye. The Minimum Bactericidal Concentration (MBC) was determined from MIC subcultures. Assays were performed in triplicate, and chlorhexidine was used as a positive control. The diameters in mm of the growth inhibition halos ranged between 7.3 and 10.7 for S. mutans, 7.3 and 10.0 for S. oralis, and 8.2 and 9.8 for S. salivarius. The MIC and MBC values obtained converged, ranging from maximum values in the presence of Linalool (1,250.0 mg/mL, 2,500.0 mg/mL and 2,500.0 mg/mL, respectively, for S. mutans, S. oralis, and S. salivarius); and minimum values with Thymol (312.5 μg/ml, 156.2 μg/mL and 156.2 μg/ml, respectively for S. mutans, S. oralis, and S. salivarius). All the tested phytochemicals displayed antibacterial activity, thus representing substances with potential applications in preventing tooth decay.

Download Full-text