Effect of “Amprolinsyl” and “Amprolium 22%” on morphological indices of blood of turkeys for eumeria invasion

The article gives data on the influence of Amprolinsyl and Amprolium 22% on morphological parameters of blood after eumirioic invasion. It has been shown that after application of amprolium 22% for the treatment of turkeys infected with eumeria invasion, gradual normalization of hematological parameters of blood begins. Thus, at the 3rd day of treatment, the number of red blood cells increased, but remained 25.1% lower, and the hemoglobin level increased, but remained at 16.3% lower, compared with clinically healthy poultry. During this period, within the limits of normal values was the value of the color index, the mass of hemoglobin in the erythrocyte and the value of hematocrit. The obtained results indicate a gradual normalization of the erythropoietic function of the bone marrow. However, the large average volume of one erythrocyte is 1.12 ± 0.07 μm against 0.96 ± 0.03 μm³ in the control, and the decrease in the average concentration of hemoglobin in one erythrocyte by 13.3% against the norm indicates an incomplete recovery of the erythropoietic bone marrow function. At day 5 of treatment with amprolium, 22% of red blood cells and hemoglobin levels increased but still remained below physiological values by 12.3% and 6.8%, respectively, compared to control. At the 10th day of the experiment, restoration of hematopoietic function of the bone marrow was observed on physiological values of erythrocytes and hemoglobin content of the blood, as well as blood leukogram. In the treatment of turkeys with "Amprolisyl", at the 5th day, the hematopoietic function of the bone marrow completely normalized, as indicated by the number of red blood cells, hemoglobin content and red blood cell index values. It is known that the values of red blood cells, namely the volume of erythrocytes, the mass and concentration of hemoglobin in them, and the color index objectively reflect the state of hematopoietic function of the bone marrow. The results of our studies indicate that in the turkey infected with imeiriosis and treated with amprolium 22%, clinical recovery occurs at day 5, and the hematopoietic function of the bone marrow is restored spontaneously in 5 days after recovery. In the treatment of turkeys with Amprolinsyl, the restoration of morphological parameters, the normalization of white blood cell leukemogram and the clinical recovery of turkeys arose on the fifth day of treatment. Thus, due to the fact that Amporolinsyl, in addition to the active ingredient, contains sparse fruit of thistle spots, which contain high levels of vitamins A and K, and trace elements – Ferum, Kuprum and Cobalt, which are directly involved in hemopoiesis, therefore, the restoration of hematopoietic function of the bone marrow comes much faster than with the application of amprolium itself 22%.

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Reversible Neutropenia Associated with Ampicillin Therapy in Pediatric Patients

Drug Intelligence & Clinical Pharmacy ◽

10.1177/106002808101501015 ◽

1981 ◽

Vol 15 (10) ◽

pp. 802-806 ◽

Cited By ~ 4

Author(s):

Kusum Kumar ◽

Ashir Kumar

Keyword(s):

Bone Marrow ◽

Blood Cell ◽

Red Blood Cells ◽

White Blood Cell ◽

Blood Cells ◽

Pediatric Patients ◽

Bone Marrow Examination ◽

High Dose ◽

Maturation Arrest ◽

High Doses

Hematologic abnormalities associated with penicillin compounds are uncommon, and neutropenia associated with ampicillin is reported even less frequently. Neutropenia developed in three pediatric patients after high-dose (150–400 mg/kg) ampicillin therapy over a period of 3 to 12 days. In all cases, the white blood cell and neutrophil counts returned towards normal within 4 to 11 days after discontinuation of the antibiotic. Bone marrow examination revealed a maturation arrest in one and slight shift to the left in the maturation of granulocytic cells in another. Other marrow components were normal. Red blood cells, reticulocytes, platelets, and hemoglobin did not show any abnormal alteration in any of the patients. Physicians administering ampicillin, particularly in high doses, should be alert to the possible development of neutropenia; however, all reported neutropenias have been reversible.

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THE EFFECT OF HEMOGLOBIN INJECTIONS ON ERYTHROPOIESIS AND ERYTHROCYTE SIZE IN RABBITS RENDERED ANEMIC BY BLEEDING

Journal of Experimental Medicine ◽

10.1084/jem.59.3.333 ◽

1934 ◽

Vol 59 (3) ◽

pp. 333-346 ◽

Cited By ~ 3

Author(s):

D. K. Miller ◽

C. P. Rhoads

Keyword(s):

Bone Marrow ◽

Red Blood Cells ◽

Blood Cells ◽

Subcutaneous Injection ◽

Color Index ◽

Free Hemoglobin ◽

Hemoglobin Solution

1. An anemia characterized by increased size of the red blood cells and a high color index was produced in rabbits by repeated bleeding and by the subcutaneous injection of stroma-free hemoglobin solution. 2. The bone marrow of these rabbits reverted to a more primitive stage than did the marrows of rabbits rendered anemic in the same manner but not treated with hemoglobin.

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HEMATOLOGICAL INDICES OF RAT ORGANISMS UNDER CONDITIONS OF OXIDATIVE STRESS AND LIPOSOMAL PREPARATION ACTION

Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University ◽

10.15421/201615 ◽

2016 ◽

Vol 6 (01) ◽

pp. 276-289 ◽

Cited By ~ 1

Author(s):

M. Khariv ◽

B. V. Gutyj ◽

V. Butsyak ◽

І. Khariv

Keyword(s):

Oxidative Stress ◽

Red Blood Cells ◽

Blood Cells ◽

Hematological Parameters ◽

Hemoglobin Concentration ◽

The Body ◽

Hemoglobin Content ◽

Hematological Indices ◽

Liposomal Drug ◽

Physiological Variables

<p>The article deals with the results of search of the influence of developed complex liposomal drug on dynamics of hematological parameters of rat organisms under conditions of simulated oxidative stress caused by the use of carbon tetrachloride. Intramuscular injection of 50% tetrachloromethane to rats at a dose of 0.25 ml per 100 g of body weight causes antigenic load on the body and leads to disruption of physiologic level of hematological indices of experimental animal organisms. This indicates the number reduce of red blood cells, hemoglobin content, hemoglobin concentration in erythrocyte, increasing the number of leukocytes, mass of hemoglobin in erythrocyte and increased of color index.</p><p>To normalize the hematological indices of rat organisms for the development of oxidative stress it is advisable to apply the liposomal drug that incorporates contains butafosfan, interferon, milk thistle and vitamins. When using liposomal drug to rats, under conditions of oxidative stress, the normalization of hematological indices comes in blood, namely on 14th day within physiological variables were indicators of the number of erythrocytes of hemoglobin content, white blood cell count and indices of red blood cells compared to controls, indicating a recovery of hematopoietic function of marrow.</p>

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On Demand Recruitment of Macrophages Is Required for Erythroid Niche Formation during Stress Erythropoiesis in the Bone Marrow

Blood ◽

10.1182/blood-2018-99-116244 ◽

2018 ◽

Vol 132 (Supplement 1) ◽

pp. 848-848

Author(s):

Verena Petzer ◽

Piotr Tymoszuk ◽

Felix Böhm ◽

Markus Seifert ◽

Sieghart Sopper ◽

...

Keyword(s):

Bone Marrow ◽

Steady State ◽

Blood Cell ◽

Red Blood Cells ◽

Red Blood Cell ◽

Blood Cells ◽

Iron Availability ◽

Stress Erythropoiesis ◽

Iron Supply ◽

Reporter Mice

Abstract Every second more than 2 million new erythrocytes are released from the bone marrow of human adults, highlighting the tremendous turn-over of these cells. In parallel to hematopoietic stem cell niches, the last stages of erythropoiesis take place in specialized bone marrow niches, termed 'erythroid niches'. Concretely, the erythroid niche is composed of a 'central macrophage' which is surrounded by erythroid progenitor cells. Regardless of steady-state or stress erythropoiesis, iron availability is, beside erythropoietin, a key factor determining erythroid output and red blood cell quality, as reflected by hemoglobin content of these cells. It is well established that systemic iron availability for erythropoiesis, in the form of iron saturated transferrin (Tf), is mainly maintained via a recycling process of senescent red blood cells, which takes place in macrophages of the reticuloendothelial system. Yet, it is still a matter of debate if also central macrophages are involved in iron supply for red blood cell development in a more direct way due to their close proximity to developing red blood cells. Using a myeloid-specific knockout mouse strain, lacking the solely known iron exporter ferroportin (Fpn; Fpnfl/flLysMCre+/+ mice) and specific reporter mice (ROSA26tdTomatofl/fl Cx3cr1CreERT2 mice), we examined the connection between iron metabolism, erythropoiesis and central macrophages. Analysis of Fpnfl/flLysMCre+/+ animals at steady state revealed microcytic anemia, higher tissue iron loading, reduced hepatic hepcidin expression and distorted erythroid precursor population distribution in the bone marrow with no significant chances in Tf saturation (Tf-Sat). The latter is giving a first hint, that local bone marrow Fpn expression on macrophages may be important for iron supply for erythropoiesis. Strikingly, further work up via flow cytometry demonstrated that disturbances seen in bone marrow erythropoiesis were accompanied by nearby loss of resident bone marrow macrophages (defined as CD11blo, F4/80pos, MerTKpos). In parallel, a CD11bhi, F4/80pos, MerTKpos population came into existence, suggesting that these cells may compensate for the loss of 'canonical' central macrophages. Attempting to explain these intriguing results, we sought to investigate differentiation pathways and turnover of bone marrow central macrophages. First, we used the ROSA26tdTomatofl/fl Cx3cr1CreERT2 monocyte-specific reporter mice and techniques of transient monocyte labelling in utero and in adult phlebotomized animals to determine the origin of central macrophages. We could show that those cells undergo constant replenishment by circulating monocytes. Notably, the rate of this process got markedly increased upon recovery from blood loss and concomitant expansion of the central macrophage population. Second, by administration of a CCR2/CCR5 inhibitor (cenicriviroc), diminishing monocyte egress from the bone marrow and tissue infiltration, we could demonstrate decreased reticulocyte count during stress erythropoiesis, thus strengthening the direct impact of macrophages to support effective erythroid output. Next, effects of stress-induced erythropoiesis were investigated in Fpnfl/flLysMCre+/+ compared to Fpnfl/flLysMCre-/- mice. Amelioration of anemia after phlebotomy was extended, microcytosis was more pronounced and reticulocyte egress was diminished but prolonged. Of interest, Fpnfl/flLysMCre+/+ mice on a diet containing an 8-times higher iron content during phlebotomy, thus transiently increasing Tf-Sat, recovered from anemia wildtype-like. These results indicate that stress erythropoiesis with a high iron demand depends, under normal iron availability, in part on central macrophages and their nursing function to overcome the increased demand of iron. Ongoing experiments aim to identify how recruited bone marrow macrophages, i.e. central macrophages, contribute to erythropoiesis during stress - if central macrophages directly supply developing erythroid cells with iron in a Tf-free fashion or, if they are suppliers of additional growth factors that work synergistically with the Tf-bound iron to drive hemoglobin production. In summary our data clearly show that macrophages need to be recruited to the bone marrow for effective erythroid output during stress erythropoiesis. Disclosures Weiss: Kymab Ltd.: Consultancy. Theurl:Kymab Ltd.: Consultancy, Research Funding.

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CLINICAL STUDIES OF THE EFFECTS OF 3,5,5, TRIMETHYLOXAZOLIDINE-2-4-DIONE (TRIDIONE®) ON THE HEMATOPOIETIC SYSTEM, LIVER AND KIDNEY

PEDIATRICS ◽

10.1542/peds.5.4.695 ◽

1950 ◽

Vol 5 (4) ◽

pp. 695-707

Author(s):

ERIC DENHOFF ◽

MAURICE W. LAUFER

Keyword(s):

Bone Marrow ◽

Blood Cell ◽

Red Blood Cells ◽

Red Blood Cell ◽

Blood Cells ◽

Blood Platelet ◽

Clot Retraction ◽

Nucleated Red Blood Cells ◽

Complete Blood Counts ◽

Nucleated Red Blood Cell

Six children receiving tridione® showed : 1. A constant syndrome of : a decline in bone marrow megakaryocyte and nucleated red blood cell elements; a low peripheral blood platelet count; and prolonged clot retraction time. 2. An occasional association of: peripheral leukopenia, toxic signs in the red blood cells and lymphocytes, and an abnormal cephalin flocculation response. In one case the bone marrow megakaryocyte response occurred alone and was temporary; in another it was temporary but associated with a persistent decline in marrow nucleated red blood cells; and in a third case both the megakaryocyte and nucleated red blood cell depression were only temporary, despite continuation of the medication. Withdrawal of medication caused a return to normal values, most rapid and marked in the bone marrow. Tests for clot retraction time and platelet counts at regular intervals in addition to complete blood counts seem indicated in all patients receiving tridione®. When available, bone marrow studies, especially of megakaryocytes, would also be of value.

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Microfluidic electrical impedance assessment of red blood cell mediated microvascular occlusion

Lab on a Chip ◽

10.1039/d0lc01133a ◽

2021 ◽

Author(s):

Yuncheng Man ◽

Debnath Maji ◽

Ran An ◽

Sanjay Ahuja ◽

Jane A Little ◽

...

Keyword(s):

Sickle Cell Disease ◽

Blood Cell ◽

Red Blood Cells ◽

Sickle Cell ◽

Red Blood Cell ◽

Blood Cells ◽

Electrical Impedance ◽

Cell Disease ◽

Blood Disorders

Alterations in the deformability of red blood cells (RBCs), occurring in hemolytic blood disorders such as sickle cell disease (SCD), contributes to vaso-occlusion and disease pathophysiology. However, there are few...

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