scholarly journals HEMATOLOGICAL INDICES OF RAT ORGANISMS UNDER CONDITIONS OF OXIDATIVE STRESS AND LIPOSOMAL PREPARATION ACTION

2016 ◽  
Vol 6 (01) ◽  
pp. 276-289 ◽  
Author(s):  
M. Khariv ◽  
B. V. Gutyj ◽  
V. Butsyak ◽  
І. Khariv
Keyword(s):  
Oxidative Stress ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
The Body ◽  
Hemoglobin Content ◽  
Hematological Indices ◽  
Liposomal Drug ◽  
Physiological Variables

<p>The article deals with the results of search of the influence of developed complex liposomal drug on dynamics of hematological parameters of rat organisms under conditions of simulated oxidative stress caused by the use of carbon tetrachloride. Intramuscular injection of 50% tetrachloromethane to rats at a dose of 0.25 ml per 100 g of body weight causes antigenic load on the body and leads to disruption of physiologic level of hematological indices of experimental animal organisms. This indicates the number reduce of red blood cells, hemoglobin content, hemoglobin concentration in erythrocyte, increasing the number of leukocytes, mass of hemoglobin in erythrocyte and increased of color index.</p><p>To normalize the hematological indices of rat organisms for the development of oxidative stress it is advisable to apply the liposomal drug that incorporates contains butafosfan, interferon, milk thistle and vitamins. When using liposomal drug to rats, under conditions of oxidative stress, the normalization of hematological indices comes in blood, namely on 14th day within physiological variables were indicators of the number of erythrocytes of hemoglobin content, white blood cell count and indices of red blood cells compared to controls, indicating a recovery of hematopoietic function of marrow.</p>

scholarly journals Гематологічні і біохімічні показники крові собак за умов застосування «Неоверму» та протекторів-антитоксикантів

2017 ◽  
Vol 19 (73) ◽  
pp. 46-49
Author(s):  
I.O. Zhukova ◽  
I.O. Kostyuk ◽  
Yu.S. Svitlychna-Kulak
Keyword(s):  
Alkaline Phosphatase ◽  
Red Blood Cells ◽  
Toxic Effect ◽  
Functional State ◽  
Blood Cells ◽  
Negative Impact ◽  
Hematological Parameters ◽  
The Body ◽  
Feed Additive ◽  
Control Group

The article presents the data on the effect of antihelmintic and imunomodulating drug «Neoverm» on the dogs’ body when the drug was administered into the stomach of the animals within three days and the results of its application in the combination with the drugs that contain antioxidant and membrane stabilizing substances. To protect the body from toxic action of «Neoverm» the drugs were used under two schemes: «E-Selenium» and «Quercetin», phospholipid hepaprotector «Lesphal» and «green buckwheat» (a feed additive – pre-ground raw buckwheat as a natural source of quercetin). The result a were compared with the control group and the group that received only «Neoverm». It has been found out that the use of «Neoverm» for the dogs both on the 7th and 14th days had a negative impact on the hematological parameters and the activity of blood enzymes that reflects the functional state of the liver. In particular, the reduction in the number of erythrocytes and the level of hemoglobin as well as the significant increase in the activity of diagnostic enzymes: alanine (ALT), asparagine (AST), gammaglutamyl (GGT) transferases and alkaline phosphatase (ALP) have been revealed. The proposed schemes to correct the toxic effect of the drug «Neoverm» in dogs led to the restoration of endogenous antitoxic resources, that is, to a gradual normalization of hematological parameters and the activity of indicatory enzymes of blood (the decrease in the activity of AST, ALT, GGT, ALP and LDH and the normalization of the number of red blood cells and hemoglobin) and that is the basis for the use of the above drugs as protectors-antioxidants when «Neoverm» is used.

scholarly journals Effect of “Amprolinsyl” and “Amprolium 22%” on morphological indices of blood of turkeys for eumeria invasion

2019 ◽  
Vol 21 (94) ◽  
pp. 157-162
Author(s):  
V. Stybel ◽  
B. Gutyj ◽  
I. Hariv ◽  
L. Slivinska ◽  
O. Prijma
Keyword(s):  
Bone Marrow ◽  
Blood Cell ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Color Index ◽  
Hematological Parameters ◽  
Morphological Parameters ◽  
Hemoglobin Content ◽  
Clinical Recovery ◽  
Physiological Values

The article gives data on the influence of Amprolinsyl and Amprolium 22% on morphological parameters of blood after eumirioic invasion. It has been shown that after application of amprolium 22% for the treatment of turkeys infected with eumeria invasion, gradual normalization of hematological parameters of blood begins. Thus, at the 3rd day of treatment, the number of red blood cells increased, but remained 25.1% lower, and the hemoglobin level increased, but remained at 16.3% lower, compared with clinically healthy poultry. During this period, within the limits of normal values was the value of the color index, the mass of hemoglobin in the erythrocyte and the value of hematocrit. The obtained results indicate a gradual normalization of the erythropoietic function of the bone marrow. However, the large average volume of one erythrocyte is 1.12 ± 0.07 μm against 0.96 ± 0.03 μm³ in the control, and the decrease in the average concentration of hemoglobin in one erythrocyte by 13.3% against the norm indicates an incomplete recovery of the erythropoietic bone marrow function. At day 5 of treatment with amprolium, 22% of red blood cells and hemoglobin levels increased but still remained below physiological values by 12.3% and 6.8%, respectively, compared to control. At the 10th day of the experiment, restoration of hematopoietic function of the bone marrow was observed on physiological values of erythrocytes and hemoglobin content of the blood, as well as blood leukogram. In the treatment of turkeys with "Amprolisyl", at the 5th day, the hematopoietic function of the bone marrow completely normalized, as indicated by the number of red blood cells, hemoglobin content and red blood cell index values. It is known that the values of red blood cells, namely the volume of erythrocytes, the mass and concentration of hemoglobin in them, and the color index objectively reflect the state of hematopoietic function of the bone marrow. The results of our studies indicate that in the turkey infected with imeiriosis and treated with amprolium 22%, clinical recovery occurs at day 5, and the hematopoietic function of the bone marrow is restored spontaneously in 5 days after recovery. In the treatment of turkeys with Amprolinsyl, the restoration of morphological parameters, the normalization of white blood cell leukemogram and the clinical recovery of turkeys arose on the fifth day of treatment. Thus, due to the fact that Amporolinsyl, in addition to the active ingredient, contains sparse fruit of thistle spots, which contain high levels of vitamins A and K, and trace elements – Ferum, Kuprum and Cobalt, which are directly involved in hemopoiesis, therefore, the restoration of hematopoietic function of the bone marrow comes much faster than with the application of amprolium itself 22%.

scholarly journals EFFECT OF DEUTERIUM WATER ON BLOOD VALUES AND DIGESTIBILITY OF NUTRIENTS OF RHESUS MACAQUE

2020 ◽  
Vol 384 (2) ◽  
pp. 22-28 ◽  
Author(s):  
N. V. Gaponov ◽  
S. V. Svistunov ◽  
N. N. Bondarenko ◽  
I. A. Romanenko
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
The Body ◽  
Control Group ◽  
Deuterium Content ◽  
Animal Blood ◽  
Constant Weight ◽  
Effect Of Water

The use of water with a modified isotopic composition in the diet of humans and animals, for example, with a reduced deuterium content, affects the rate of biological processes. In this regard, the targeted formation of the isotopic D / H gradient in the body can be used to increase its adaptive properties to external influences. Primates have a high anatomical and physiological similarity with humans, therefore, are an indispensable model for reproducing various pathological and toxic conditions in humans. The aim of the study was to study the effect of water with a low deuterium content on the hematological and biochemical blood parameters of rhesus monkeys and on the digestibility of nutrients of the diet. In the control group, primates consumed water with a natural regional content of deuterium equal to 150 mg / l, and in the experimental group - with reduced to 50 mg/l. The duration of the experiment was 35 days. The material for the study was venous blood, serum and feces of primates. Blood was drawn from monkeys before the use of deuterium depleted water and after the completion of the experiment. All blood samples (2.5-3.0 ml) were taken from the cubital or femoral veins of animals on an empty stomach and stabilized with a heparin solution. Laboratory studies of animal blood were performed on an automatic hematological analyzer (Beckman Coulter, USA) of the CoulterAcT 5diffCP brand. In order to study the effect of water with a reduced deuterium content on hematological parameters, we determined the number of red blood cells, white blood cells, platelets, hemoglobin concentration, hematocrit, the average volume of red blood cells, red blood cell anisocytosis, and erythrocyte sedimentation rate (ESR). The metabolism trial was divided into two periods. Preliminary - to exclude the influence of previous feeding and accustoming primates to cage conditions of keeping. Experimental - conducting a thorough accounting of consumed feed, excreted excrement. Feces were collected daily at the same time (morning and evening), weighed and ground in a mortar. At each collection, 50% of the homogenized mass was taken for analysis. The collected portions were stored in the refrigerator. After the end of the experimental (accounting) period in the collected feces, the initial moisture was determined by drying at 60-70 ℃ to constant weight. The resulting air-dry mass was thoroughly ground and analyzed. It has been found that the introduction of water with a reduced deuterium content into the diet of primates has a positive effect on feed conversion rate and blood biochemical parameters.

scholarly journals Analisis faktor-faktor yang berhubungan dengan kadar hemoglobin pada ibu hamil

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
I. G.B. Ngurah Rai ◽  
Shriley E.S. Kawengian ◽  
Nelly Mayulu
Keyword(s):  
Red Blood Cells ◽  
Pregnant Women ◽  
Blood Cells ◽  
Employment Status ◽  
Sampling Method ◽  
Hemoglobin Concentration ◽  
The Body ◽  
P Value ◽  
Cross Sectional ◽  
The Status

Abstrak: Anemia merupakan suatu kondisi di mana jumlah dan ukuran sel darah merah, atau konsentrasi hemoglobin di bawah batas nilai normal, akibatnya dapat merusak kapasitas darah untuk mengangkut oksigen yang di hantarkan ke seluruh tubuh. Ibu hamil merupakan salah satu kelompok yang berisiko mengalami anemia karena peningkatan volume plasma yang berakibat pengenceran kadar hemoglobin (hb) tanpa perubahan bentuk sel darah merah. Ibu hamil dianggap mengalami anemia bila kadar Hb-nya <11,0 g/dL. Anemia dapat memperburuk proses kelahiran.. Penelitian ini bertujuan untuk melihat apakah ada hubungan antara status pekerjaan, pendidikan dan penghasilan suami dengan kadar hemoglobin. Pengambilan data dilakukan dengan menggunakan metode cross-sectional. Penelitian ini dilakukan pada bulan September-November 2016 di Puskesmas Bahu, Ranotana, Tuminting, Kombos dan Paniki. Pengambilan sampel menggunakan metode total sampling dengan jumlah responden sebanyak 49 orang. Dari hasil penelitian diperoleh nilai p masing-masing sebesar 0,362 status pekerjaan dengan kadar hb, 0,688 pendidikan dengan kadar hb dan 0,650 penghasilan suami dengan kadar hb yakni lebih besar dibanddingkan angka α=0,05. Kesimpulan: Tidak ada hubungan antara kadar hemoglobin (hb) dengan status pekerjaan, pendidikan dan penghasilan suami.Kata kunci: kadar hemoglobin, anemia, ibu hamil. Abstract: Anemia is a condition which the number and size of red blood cells or the hemoglobin concentration below the normal value, that can damage the capacity of the blood to transport oxygen throughout the body. Pregnant women is one of the groups at risk of anemia. high risk cause of increased plasma volume resulting in hemoglobin dilution without change in shape of the red blood cells. Pregnant women considered to have anemia when hemoglobin concentrate counted below 11.0g/dL and can worsen of giving birth progress. This study aims to determine whether there is a relation between employment status, education, and the husband’s income with the hemoglobin level in pregnant women. The study design used cross-sectional research by total sampling method to 49 people during september-november 2016 in Bahu, Ranotana, Tuminting, Kombos, and Paniki public health center. Result of research acquired the p-value of 0.362, respectively the status of the job with hemoglobin rate 0.688, education level and hemoglobin rate 0.650, the husband’s income level with hemoglobin rate is bigger than the α=0.05. Conclusion: There is no relation between the level of hemoglobin with employment status, education and husband’s income. Keywords: hemoglobin, anemia, pregnancy woman

scholarly journals METABOLISM OF HOMOCYSTEIN AND GLUTATHIONE IN ERYTHROCYTES IN HYPOTHERMAL STORAGE

2019 ◽  
Vol 19 (1) ◽  
pp. 115-118
Author(s):  
V.V. Ramazanov ◽  
E.L. Volovelskaya ◽  
S.V. Rudenko ◽  
A.Yu. Semenchenko ◽  
V.A. Bondarenko
Keyword(s):  
Oxidative Stress ◽  
Amino Acids ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Redox Homeostasis ◽  
The Body ◽  
Antioxidant Potential ◽  
Vascular Pathology ◽  
Hemoglobin Oxidation ◽  
Stimulation Of

When hypothermic storage of red blood cellsб there is a decrease in the level of glutathione and an increase in the concentration of homocysteine, at the same time, inclusion in the medium of substrate amino-acids of the synthesis of glutathione activates the utilization of this cytotoxin. Moreover, stimulation of the synthesis of glutathione reduces the loss of membrane proteins and lowers the intensity of hemoglobin oxidation that ensures the preservation of the osmotic stability of erythrocytes. Red blood cells as a component of the body redox homeostasis absorb and neutralize reactive oxygen and nitrogen species, which normally determine a certain antioxidant potential and are not enough to maintain the structural and functional state of red blood cells after isolation from donor blood and storage. Oxidative stress in cells, including red blood cells, in various pathologies usually causes a decrease in the level of glutathione and an impairment of its synthesis that leads to an increase in the production of homocysteine ​​and its entry into the blood plasma. Homocysteine ​​is a cytotoxic amino acid that enhances the production of superoxide, limits the bioavailability of nitric oxide and causes the development of vascular pathology. The data presented in the review indicate that stimulation of the antioxidant potential of erythrocytes during the storage may prevent the development of oxidative stress and inflammation during subsequent transfusion. In addition, the preservation of the viability indicators of various cells during cryopreservation in a medium with substrate amino-acids shows that stimulation of the antioxidant potential contributes to an increase in the cell resistance to the damaging factors of freeze-thawing.

scholarly journals Alteration of oxidative stress parameters in red blood cells of rats after chronic in vivo treatment with cisplatin and selenium

2011 ◽  
Vol 63 (4) ◽  
pp. 991-999 ◽  
Author(s):  
Snezana Markovic ◽  
Jovana Zizic ◽  
Dragana Djacic ◽  
Ana Obradovic ◽  
Milena Curcic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Hematological Parameters ◽  
Albino Rats ◽  
Protective Effects ◽  
Oxidative Stress Parameters ◽  
And Oxidative Stress ◽  
Stress Parameters

In this study we evaluated the possible protective effects of selenium (Se) on hematological and oxidative stress parameters in rats chronically treated with cisplatin (cisPt). Four groups of Wistar albino rats were examined: a control, untreated rats (I), rats treated with Se (II), rats treated with cisPt (III), and rats treated with Se and cisPt (IV). All animals were treated for 5 days successively and killed 24 h after the last treatment. Hematological and oxidative stress parameters were followed in whole blood and red blood cells (RBC). Results showed that the chronic application of Se was followed by a higher number of reticulocytes and platelets, increased lipid peroxidation and GSH content in the RBC. Cisplatin treatment induced depletion of RBC and platelet numbers and an elevation of the superoxide anion, nitrites and glutathione levels. Se and cisPt co-treatment was followed by an elevation of the hematological parameters and the recovery of the glutathione status when compared to the control and cisPt-treated rats.

scholarly journals Hemoglobin Oxidation in Stored Blood Accelerates Hemolysis and Oxidative Injury to Red Blood Cells

2020 ◽  
Vol 12 (04) ◽  
pp. 244-249
Author(s):  
Ibrahim Mustafa ◽  
Tameem Ali Qaid Hadwan
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Morphological Changes ◽  
Hemoglobin Concentration ◽  
Red Cell Membrane ◽  
Rbc Membrane ◽  
Hemoglobin Oxidation ◽  
Rbc Indices ◽  
Methemoglobin Formation ◽  
Stored Blood

Abstract Introduction Maintaining blood supply is a challenge in blood banks. Red blood cells (RBCs) stored at 4°C experience issues of biochemical changes due to metabolism of cells, leading to changes collectively referred to as “storage lesions.” Oxidation of the red cell membrane, leading to lysis, contributes to these storage lesions. Methods Blood bags with CPD-SAGM stored at 4°C for 28 days were withdrawn aseptically on days 1, 14, and 28. Hematology analyzer was used to investigate RBC indices. Hemoglobin oxidation was studied through spectrophotometric scan of spectral change. RBC lysis was studied with the help of Drabkin's assay, and morphological changes were observed by light and scan electron microscopy. Results RBCs show progressive changes in morphology echinocytes and spherocytes on day 28. There was 0.85% RBC lysis, an approximately 20% decrease in percentage oxyhemoglobin, and a 14% increase in methemoglobin formation, which shows hemoglobin oxidation on day 28. Conclusions Oxidative damage to RBC, with an increase in storage time was observed in the present study. The observed morphological changes to RBC during the course of increased time shows that there is progressive damage to RBC membrane and a decrease in hemoglobin concentration; percentage RBC lysis is probably due to free hemoglobin and iron.

scholarly journals Regulation of intracellular glutathione levels in erythrocytes infected with chloroquine-sensitive and chloroquine-resistant Plasmodium falciparum

2002 ◽  
Vol 368 (3) ◽  
pp. 761-768 ◽  
Author(s):  
Svenja MEIERJOHANN ◽  
Rolf D. WALTER ◽  
Sylke MÜLLER
Keyword(s):  
Oxidative Stress ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Differential Susceptibility ◽  
Protozoan Parasite ◽  
Chloroquine Resistance ◽  
Glutathione Metabolism ◽  
Glutathione Synthesis ◽  
Intracellular Glutathione ◽  
Glutamylcysteine Synthetase

Malaria is one of the most devastating tropical diseases despite the availability of numerous drugs acting against the protozoan parasite Plasmodium in its human host. However, the development of drug resistance renders most of the existing drugs useless. In the malaria parasite the tripeptide glutathione is not only involved in maintaining an adequate intracellular redox environment and protecting the cell against oxidative stress, but it has also been shown that it degrades non-polymerized ferriprotoporphyrin IX (FP IX) and is thus implicated in the development of chloroquine resistance. Glutathione levels in Plasmodium-infected red blood cells are regulated by glutathione synthesis, glutathione reduction and glutathione efflux. Therefore the effects of drugs that interfere with these metabolic processes were studied to establish possible differences in the regulation of the glutathione metabolism of a chloroquine-sensitive and a chloroquine-resistant strain of Plasmodiumfalciparum. Growth inhibition of P. falciparum 3D7 by d,l-buthionine-(S,R)sulphoximine (BSO), an inhibitor of γ-glutamylcysteine synthetase (γ-GCS), and by Methylene Blue (MB), an inhibitor of gluta thione reductase (GR), was significantly more pronounced than inhibition of P.falciparum Dd2 growth by these drugs. These results correlate with the higher levels of total glutathione in P. falciparum Dd2. Short-term incubations of Percoll-enriched trophozoite-infected red blood cells in the presence of BSO, MB and N,N1-bis(2-chloroethyl)-N-nitrosourea and subsequent determinations of γ-GCS activities, GR activities and glutathione disulphide efflux revealed that maintenance of intracellular glutathione in P. falciparum Dd2 is mainly dependent on glutathione synthesis whereas in P. falciparum 3D7 it is regulated via GR. Generally, P. falciparum Dd2 appears to be able to sustain its intracellular glutathione more efficiently than P. falciparum 3D7. In agreement with these findings is the differential susceptibility to oxidative stress of both parasite strains elicited by the glucose/glucose oxidase system.

scholarly journals Using redox potential as a feasible marker for banked blood quality and the state of oxidative stress in stored red blood cells

2021 ◽  
Author(s):  
Rodney C. Daniels ◽  
Hyesun Jun ◽  
Robertson D. Davenport ◽  
Maryanne M. Collinson ◽  
Kevin R. Ward
Keyword(s):  
Oxidative Stress ◽  
Redox Potential ◽  
Red Blood Cells ◽  
Blood Cells ◽  
The State

Clock gene-independent daily regulation of haemoglobin oxidation in red blood cells

2021 ◽  
Author(s):  
Andrew D. Beale ◽  
Priya Crosby ◽  
Utham K. Valekunja ◽  
Rachel S. Edgar ◽  
Johanna E. Chesham ◽  
...  
Keyword(s):  
Circadian Rhythms ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Human Subjects ◽  
Developmental Regulation ◽  
Physiological Role ◽  
Cell Types ◽  
The Body

AbstractCellular circadian rhythms confer daily temporal organisation upon behaviour and physiology that is fundamental to human health and disease. Rhythms are present in red blood cells (RBCs), the most abundant cell type in the body. Being naturally anucleate, RBC circadian rhythms share key elements of post-translational, but not transcriptional, regulation with other cell types. The physiological function and developmental regulation of RBC circadian rhythms is poorly understood, however, partly due to the small number of appropriate techniques available. Here, we extend the RBC circadian toolkit with a novel biochemical assay for haemoglobin oxidation status, termed “Bloody Blotting”. Our approach relies on a redox-sensitive covalent haem-haemoglobin linkage that forms during cell lysis. Formation of this linkage exhibits daily rhythms in vitro, which are unaffected by mutations that affect the timing of circadian rhythms in nucleated cells. In vivo, haemoglobin oxidation rhythms demonstrate daily variation in the oxygen-carrying and nitrite reductase capacity of the blood, and are seen in human subjects under controlled laboratory conditions as well as in freely-behaving humans. These results extend our molecular understanding of RBC circadian rhythms and suggest they serve an important physiological role in gas transport.

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