scholarly journals Application infusion drugs based on sorbitol for normalization of aminotransferase activity blood cancer patients operated cardia of the stomach

2020 ◽  
pp. 29-30
Author(s):  
B.R. Dzis ◽  
S.V. Prymak ◽  
R.P. Dzis ◽  
V.L. Novak ◽  
M.P. Dzisiv ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Postoperative Period ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Proximal Gastrectomy ◽  
Early Postoperative Period ◽  
Aminotransferase Activity ◽  
Cardiac Cancer ◽  
Intravenous Infusions ◽  
In Blood Plasma

Objective. To study the effect of intravenous infusions of Rheosorbilact on the activity of alanine aminotransferase and aspartate aminotransferase in the plasma of operated patients with gastric cardiac cancer after proximal gastrectomy. Materials and methods. Alanine aminotransferase and aspartate aminotransferase activity levels were studied in plasma of 40 operated patients with gastric cardiac cancer after proximal gastrectomy immediately after surgery, on the 1st, 2nd, 3rd, 5th day after intravenous infusions of the drug. The infusion of Rheosorbilact was administered intravenously, drip, for 5 days at a rate of 40 drops per minute. The daily dose of the drug was 1000.0 ml. Results. In patients with gastric cardiac cancer after proximal gastrectomy in the first days after surgery revealed changes in aminotransferases, which are accompanied by an increase in the activity of alanine aminotransferase and aspartate aminotransferase in blood plasma. To correct the activity of plasma aminotransferases in such patients, Rheosorbilact was administered intravenously immediately after surgery. After repeated intravenous infusions of Rheosorbilact for 5 days, a significant decrease in the activity of alanine aminotransferase and aspartate aminotransferase in blood plasma in the operated patients was revealed. Conclusions. In the first days after proximal gastrectomy in patients with gastric cardiac cancer, an increase in the activity of aminotransferases in blood plasma was revealed. Repeated intravenous infusions of Rheosorbilact normalize the activity of alanine aminotransferase and aspartate aminotransferase in the blood plasma of such patients in the early postoperative period. Infusion drug Rheosorbilact is recommended for widespread medical use in patients with cardiac gastric cancer after proximal gastrectomy, especially in the early postoperative period.

scholarly journals Zinc as a feed supplement and its impact on plasma cholesterol concentrations in breeding cocks

2011 ◽  
Vol 80 (3) ◽  
pp. 281-285 ◽  
Author(s):  
Tomáš Parák ◽  
Eva Straková
Keyword(s):  
Blood Plasma ◽  
Total Cholesterol ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Plasma Cholesterol ◽  
Control Group ◽  
Total Proteins ◽  
Zinc Compounds ◽  
The Impact ◽  
In Blood Plasma

The aim of this work was to verify the impact of feed supplemented with selected inorganic and organic zinc compounds on the total cholesterol concentrations and other blood plasma indices in breeding cocks. A total of 250 RIR 05 breeding cocks, 9 weeks old, were used. The cocks were divided into 5 groups of 50 animals each (four experimental groups and one control group). Cocks were fed a commercial feed mixture specifically for breeding cocks, containing 30.4 mg·kg-1 of zinc. The feed for experimental groups of cocks was fortified to 100 mg Zn·kg-1: zinc sulphate in first group, zinc oxide in second group, fodder yeast (Minvital Zn) in third group and Bioplex Zn in fourth group. Blood samples for biochemical examination were taken from the basilic vein. The contents of total cholesterol (Chol), total proteins (TP), glucose (Glu), aspartate aminotransferase (AST), alanine aminotransferase (ALT), calcium (Ca), phosphorus (P) and magnesium (Mg) in blood plasma were spectrophotometrically measured using a biochemical analyser, Cobas EMira, and commercial kits (Biovendor a.s., Czech Republic). In the 15th week of age, third and fourth groups showed a significant (P ≤ 0.05) and highly significant (P ≤ 0.01) decrease of total cholesterol in blood plasma compared to the control. In the 20th and 25th week of age, all of the experimental groups showed a significantand highly significant (P ≤ 0.01) decrease of total cholesterol in blood plasma compared to the control. Other monitored indices (total proteins, glucoses, aspartate aminotransferase, alanine aminotransferase, calcium, phosphorus and magnesium) did not reveal any significant changes between the experimental and the control groups. The presented work provides the first available experimental evidence regarding the impact of zinc supplementation on the cholesterol levels in blood plasma of breeding cocks.

scholarly journals PECULIARITIES OF BLOOD BIOCHEMICAL DISORDERS IN EXPERIMENTAL PULMONARY EMPHYSEMA

2021 ◽  
Vol 29 (2) ◽  
pp. 69-71
Author(s):  
V. I. Korzhov ◽  
◽  
B. O. Osipenko ◽  
M. P. Budennaya ◽  
M. G. Palivoda
Keyword(s):  
Blood Plasma ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Pulmonary Emphysema ◽  
Total Bilirubin ◽  
Experimental Studies ◽  
Ether Anesthesia ◽  
Intratracheal Administration ◽  
White Rats ◽  
In Blood Plasma

Experimental studies are among the important and promising approaches in the studying of the pathogenesis of pulmonary emphysema (PE). They make it possible to trace and understand the sequence of metabolic disorders wich occur in this condition. The aim of the trial was to study the nature of biochemical changes in blood plasma during the course of experimental PE. Materials and methods. The study was carried out on 36 sexually mature, outbred, white rats of both sexes, weighing 180-200 g, which were kept on a standard vivarium diet. Experimental papain PE in white rats was induced by a single intratracheal administration of 0.5 ml of papain solution (Merck KGaA, Germany) at a dose of 100 mg / kg body weight under light ether anesthesia. The rats were withdrawn from the experiment by decapitation under light ether anesthesia 10, 20, 30, 50 and 60 days after the onset of the disease. Biochemical studies of blood plasma - the content of alanine aminotransferase (ALT), aspartate aminotransferase (AST), protein, urea, creatinine, total bilirubin, glucose were carried out using automatic biochemical analyzer Selectra Pro-M (Netherlands). Results. It was found that 10 days after the onset of the disease, the levels of ALT and AST significantly decreased by 37%. The trend towards a decrease in ALT persisted after 20, 30 and 60 days; AST � after 20, 30, 50 and 60 days. It was found that the blood plasma protein significantly decreased 10 and 20 days after the onset of the disease by 33% and 38%, respectively. At the same time, the urea also decreased after 10 days by 36% and by 32% after 20 days. A similar trend of changes was found when studying the level of creatinine in blood plasma. After 10 and 20 days, creatinine decreased by 36% and 37%, respectively. As for total bilirubin, its blood plasma contentration increased by 68% after 10 days, and by 38% after 20 days. There were no changes in plasma glucose levels 10, 20, 30, 50 and 60 days after the onset of the disease. Conclusions. Experimental PE is characterized by the most pronounced changes in blood plasma biochemical parameters 10 and 20 days after the onset of the disease, which is manifested by a decrease of aminotransferases � ALT and AST, a decrease in total protein, urea, creatinine, and an increase of total bilirubin. Key words: experimental pulmonary emphysema, alanine aminotransferase, aspartate aminotransferase, protein, urea, creatinine, bilirubin, glucose. U

Low-Level Alanine Aminotransferase Elevations in Osteoarthritis Patients within Approximately 2 Weeks of Treatment Initiation with Daily Acetaminophen: A Retrospective Analysis Evaluating Subsequent Alanine Aminotransferase Activity on Continued Acetaminophen Therapy

2013 ◽  
Vol 5 ◽  
pp. CMT.S11120
Author(s):  
Edwin K. Kuffner ◽  
Kimberly M. Cooper ◽  
Jeffrey S. Baggish ◽  
Joseph M. Lynch ◽  
Brenda A. Zimmerman ◽  
...  
Keyword(s):  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Treatment Initiation ◽  
Reference Range ◽  
Aminotransferase Activity ◽  
Upper Limit ◽  
Therapy Initiation ◽  
Almost All ◽  
Alanine Aminotransferase Activity ◽  
Alt Activity

Published analyses have noted elevated alanine aminotransferase (ALT) activity in patients taking up to 4000 mg/day of acetaminophen. Data from 3 osteoarthritis trials of acetaminophen 3900–4000 mg/day in which ALT and aspartate aminotransferase (AST) were recorded within approximately 2 weeks of therapy initiation were retrospectively analyzed. Patients with baseline ALT or AST above the upper limit of the reference range (ULRR) were excluded. Among 466 patients, 376 (80.7%) had no ALT elevations within approximately 2 weeks of treatment initiation. Elevations >1.5 and >3.0 times ULRR occurred in 4.5% and 0.9% of patients, respectively. Elevations were transient as most resolved (72.9%) or declined (22.4%) with continued treatment beyond 2 weeks. Within approximately 2 weeks of therapy initiation, no patient had ALT > 5 times ULRR or ALT > 3 times ULRR combined with bilirubin >2 times ULRR. ALT elevations were transient and asymptomatic; almost all resolved or declined while on continued therapy and appear not to be clinically important. Clinical trials.gov ID numbers: NCT00240799, NCT00240786

scholarly journals Selective inhibition of alanine aminotransferase and aspartate aminotransferase in rat hepatocytes

1984 ◽  
Vol 220 (3) ◽  
pp. 707-716 ◽  
Author(s):  
N W Cornell ◽  
P F Zuurendonk ◽  
M J Kerich ◽  
C B Straight
Keyword(s):  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Rat Hepatocytes ◽  
Selective Inhibition ◽  
Enoic Acid ◽  
Urea Synthesis ◽  
Aminotransferase Activity ◽  
Glucose Synthesis ◽  
Alanine Aminotransferase Activity

Experiments were conducted with intact rat hepatocytes to identify inhibitors and incubation conditions that cause selective inhibition of alanine aminotransferase or aspartate aminotransferase. Satisfactory results were obtained by preincubating cells with L-cycloserine or L-2-amino-4-methoxy-trans-but-3-enoic acid in the absence of added substrates. When cells were incubated for 20 min with 50 microM-L-cycloserine, alanine aminotransferase activity was decreased by 90%, whereas aspartate aminotransferase was inhibited by 10% or less. On subsequent incubation, synthesis of glucose and urea from alanine was strongly inhibited, but glucose synthesis from lactate was unaffected. L-2-Amino-4-methoxy-trans-but-3-enoic acid (400 microM) in hepatocyte incubations caused 90-95% inactivation of aspartate aminotransferase, but only 15-30% loss of alanine aminotransferase activity. After preincubation with the inhibitor, glucose synthesis from lactate was almost completely blocked; with alanine as the substrate, gluconeogenesis was unaffected, and urea synthesis was only slightly decreased. By comparison with preincubation with inhibitors, simultaneous addition of substrates (alanine; lactate plus lysine) and inhibitors (cycloserine; aminomethoxybutenoic acid) resulted in smaller decreases in aminotransferase activities and in metabolic rates. Other compounds were less satisfactory as selective inhibitors. Ethylhydrazinoacetate inactivated the two aminotransferases to similar extents. Vinylglycine was almost equally effective in blocking the two enzymes in vitro, but was a very weak inhibitor when used with intact cells. Concentrations of DL-propargylglycine (4 mM) required to cause at least 90% inhibition of alanine aminotransferase in hepatocytes also caused a 16% decrease in aspartate aminotransferase. When tested in vitro, alanine aminotransferase was, as previously reported by others, more sensitive to inhibition by amino-oxyacetate than was aspartate aminotransferase, but in liver cell incubations the latter enzyme was more rapidly inactivated by amino-oxyacetate.

scholarly journals The influence of potassium and calcium ions on nitrogen metabolism of cucumber seedlings

2014 ◽  
Vol 50 (3) ◽  
pp. 437-447
Author(s):  
Genowefa Kubik-Dobosz
Keyword(s):  
Glutamate Dehydrogenase ◽  
Nitrogen Metabolism ◽  
Calcium Ions ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Developmental Phase ◽  
Aminotransferase Activity ◽  
Protein Nitrogen ◽  
Cucumber Seedlings ◽  
Plant Organ

It was found that K<sup>+</sup> or Ca<sup>2+</sup> deficiency in a nitrate or ammonium medium increased the amount of accumulated total and non-protein nitrogen in some organs of cucumber seedlings, as also caused changes in accumulated potassium and calcium. Lack of K<sup>+</sup> or Ca<sup>2+</sup> in a medium which did not contain nitrogen led to an increased level of glutamate dehydrogenase, alanine aminotransferase and aspartate aminotransferase activity in the cotyledons and roots of cucumbers. Similar changes in the activity of these enzymes were noted in certain organs of seedlings growing in nitrate or ammonium medium with decreased K<sup>+</sup> or Ca<sup>2+</sup> contents, although the magnitude of these changes depended upon the applied dosage of these cations, the form of mineral nitrogen, developmental phase of plants and the plant organ dealt with.

The Carbon Sorbents Use in Critical States of the Organism

10.12737/7274 ◽  
2014 ◽  
Vol 21 (4) ◽  
pp. 80-84
Author(s):  
Долгих ◽  
T. Dolgikh ◽  
Лихолобов ◽  
V. Likholobov ◽  
Пьянова ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Postoperative Period ◽  
Inflammatory Cytokine ◽  
Abdominal Cavity ◽  
Early Postoperative Period ◽  
Carbon Sorbent ◽  
Interleukin 4 ◽  
Surgical Removal ◽  
Toxic Substances ◽  
Purulent Peritonitis

Many modern sorbents have low sorption activity toward toxic substances of protein nature. Sorption selectivity for protein compounds can be enhanced by chemical modification of the sorbent surface withpolyvinylpyrrolidone. At the first stage of the study, 58 patients after surgical removal of abdominal cavity tumors were examined and treated. In 28 patients, the postoperative period was complicated with diffuse purulent peritonitis. So, the complex therapy of these patients was supplemented with 1-2 hemosorption sessions using a VNIITU-1 carbon sorbent in a sterile saline solution. The sorbent, which was awarded with a gold medal of the International Salon of Innovations and In-vestments, has a meso-porous surface that allows toxic substances of low and moderate molecular weight to be removed from the human body. The hemosorption sessions substantially reduced hematological, immunological and biochemical disorders and ultimately decreased mortality in the early postoperative period by a factor of 3.7. The second stage of the study was aimed to develop a molded carbon sorbent based on VNIITU-1 for selective sorption of pro-inflammatory cytokines form blood plasma. Bench testing was performed with blood plasma obtained during plasmapheresis from 15 patients with acute pancreatitis complicated with pancreonecrosis and diffuse purulent peritonitis. Blood plasma perfusion was carried out on a Unirol-1 device using the 10 cm3 columns filled with the sorbent, at a rate of 15 ml/min and a plasma/sorbent ratio of 10/1. Overall, 50 ml of plasma was passed through the column. The TNFɑ, IL-1β, IL-4, and IgA contents were determined in blood plasma before and after the sorption. The modified hemosorbent VNIITU-1-PVP showed much higher sorption capacity and selectivity than the initial VNIITU-1. This concerns pro-inflammatory cytokine interleukin-1β: its concentration decreased by a factor of 33.5 and TNF-α was virtually absent. The level of anti-inflammatory cytokine interleukin-4 was nearly twofold higher as compared to blood plasma that was passed through VNIITU-1. The IgA content remained virtually unchanged.

scholarly journals Application of sorbitol-based infusion preparation for normalization of biochemical indicators in the blood in surgery patients with direct rectal cancer

2020 ◽  
pp. 17-17
Author(s):  
Ye.S. Varivoda ◽  
I.Ya. Orishchin ◽  
V.M. Lutsiv ◽  
V.Ye. Varivoda ◽  
B.R. Dzis ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Rectal Cancer ◽  
Blood Glucose ◽  
Anterior Resection ◽  
Postoperative Period ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Early Postoperative Period ◽  
Intravenous Infusions ◽  
Complex Drug

Objective. To study the effect of intravenous infusions of the complex drug Rheosorbilaсt polyfunctional action on the biochemical parameters in the blood in operated patients with rectal cancer after anterior resection of the rectum in the early postoperative period. Materials and methods. The study of biochemical parameters in the blood of 30 patients with colorectal cancer was performed in the first days after surgery, and on the 2nd, 3rd, 5th day after administration of the drug Rheosorbilact. The drug was administered intravenously at a rate of 40 drops per minute. The daily dose of Rheosorbilact was 800.0 ml. Results. As a result of biochemical studies in patients with colorectal cancer, in the first days after anterior resection of the rectum revealed a decrease in total protein, increased concentrations of urea, creatinine and blood glucose. To correct the biochemical parameters in the blood of such patients immediately after surgery was administered intravenous infusion of Rheosorbilact. After repeated intravenous infusions of Rheosorbilact for 5 days, a significant increase in total protein content, a significant decrease in the concentration of urea, creatinine and blood glucose in the operated patients was found. Therefore, multiple intravenous infusions of the complex drug Rheosorbilact multifunctional action lead to the normalization of biochemical parameters in the blood. Conclusions. In patients with rectal cancer after anterior resection of the rectum in the first days after surgery revealed a decrease in total protein, increased concentrations of urea, creatinine and blood glucose. Repeated intravenous infusions of the complex drug Rheosorbilact multifunctional action normalize the biochemical parameters in the blood in operated patients with rectal cancer after anterior resection of the rectum in the early postoperative period. Rheosorbilact infusion is recommended for widespread medical use in such patients, especially in the early postoperative period.

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