scholarly journals Zinc as a feed supplement and its impact on plasma cholesterol concentrations in breeding cocks

2011 ◽  
Vol 80 (3) ◽  
pp. 281-285 ◽  
Author(s):  
Tomáš Parák ◽  
Eva Straková
Keyword(s):  
Blood Plasma ◽  
Total Cholesterol ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Plasma Cholesterol ◽  
Control Group ◽  
Total Proteins ◽  
Zinc Compounds ◽  
The Impact ◽  
In Blood Plasma

The aim of this work was to verify the impact of feed supplemented with selected inorganic and organic zinc compounds on the total cholesterol concentrations and other blood plasma indices in breeding cocks. A total of 250 RIR 05 breeding cocks, 9 weeks old, were used. The cocks were divided into 5 groups of 50 animals each (four experimental groups and one control group). Cocks were fed a commercial feed mixture specifically for breeding cocks, containing 30.4 mg·kg-1 of zinc. The feed for experimental groups of cocks was fortified to 100 mg Zn·kg-1: zinc sulphate in first group, zinc oxide in second group, fodder yeast (Minvital Zn) in third group and Bioplex Zn in fourth group. Blood samples for biochemical examination were taken from the basilic vein. The contents of total cholesterol (Chol), total proteins (TP), glucose (Glu), aspartate aminotransferase (AST), alanine aminotransferase (ALT), calcium (Ca), phosphorus (P) and magnesium (Mg) in blood plasma were spectrophotometrically measured using a biochemical analyser, Cobas EMira, and commercial kits (Biovendor a.s., Czech Republic). In the 15th week of age, third and fourth groups showed a significant (P ≤ 0.05) and highly significant (P ≤ 0.01) decrease of total cholesterol in blood plasma compared to the control. In the 20th and 25th week of age, all of the experimental groups showed a significantand highly significant (P ≤ 0.01) decrease of total cholesterol in blood plasma compared to the control. Other monitored indices (total proteins, glucoses, aspartate aminotransferase, alanine aminotransferase, calcium, phosphorus and magnesium) did not reveal any significant changes between the experimental and the control groups. The presented work provides the first available experimental evidence regarding the impact of zinc supplementation on the cholesterol levels in blood plasma of breeding cocks.

scholarly journals Application infusion drugs based on sorbitol for normalization of aminotransferase activity blood cancer patients operated cardia of the stomach

2020 ◽  
pp. 29-30
Author(s):  
B.R. Dzis ◽  
S.V. Prymak ◽  
R.P. Dzis ◽  
V.L. Novak ◽  
M.P. Dzisiv ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Postoperative Period ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Proximal Gastrectomy ◽  
Early Postoperative Period ◽  
Aminotransferase Activity ◽  
Cardiac Cancer ◽  
Intravenous Infusions ◽  
In Blood Plasma

Objective. To study the effect of intravenous infusions of Rheosorbilact on the activity of alanine aminotransferase and aspartate aminotransferase in the plasma of operated patients with gastric cardiac cancer after proximal gastrectomy. Materials and methods. Alanine aminotransferase and aspartate aminotransferase activity levels were studied in plasma of 40 operated patients with gastric cardiac cancer after proximal gastrectomy immediately after surgery, on the 1st, 2nd, 3rd, 5th day after intravenous infusions of the drug. The infusion of Rheosorbilact was administered intravenously, drip, for 5 days at a rate of 40 drops per minute. The daily dose of the drug was 1000.0 ml. Results. In patients with gastric cardiac cancer after proximal gastrectomy in the first days after surgery revealed changes in aminotransferases, which are accompanied by an increase in the activity of alanine aminotransferase and aspartate aminotransferase in blood plasma. To correct the activity of plasma aminotransferases in such patients, Rheosorbilact was administered intravenously immediately after surgery. After repeated intravenous infusions of Rheosorbilact for 5 days, a significant decrease in the activity of alanine aminotransferase and aspartate aminotransferase in blood plasma in the operated patients was revealed. Conclusions. In the first days after proximal gastrectomy in patients with gastric cardiac cancer, an increase in the activity of aminotransferases in blood plasma was revealed. Repeated intravenous infusions of Rheosorbilact normalize the activity of alanine aminotransferase and aspartate aminotransferase in the blood plasma of such patients in the early postoperative period. Infusion drug Rheosorbilact is recommended for widespread medical use in patients with cardiac gastric cancer after proximal gastrectomy, especially in the early postoperative period.

scholarly journals PECULIARITIES OF BLOOD BIOCHEMICAL DISORDERS IN EXPERIMENTAL PULMONARY EMPHYSEMA

2021 ◽  
Vol 29 (2) ◽  
pp. 69-71
Author(s):  
V. I. Korzhov ◽  
◽  
B. O. Osipenko ◽  
M. P. Budennaya ◽  
M. G. Palivoda
Keyword(s):  
Blood Plasma ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Pulmonary Emphysema ◽  
Total Bilirubin ◽  
Experimental Studies ◽  
Ether Anesthesia ◽  
Intratracheal Administration ◽  
White Rats ◽  
In Blood Plasma

Experimental studies are among the important and promising approaches in the studying of the pathogenesis of pulmonary emphysema (PE). They make it possible to trace and understand the sequence of metabolic disorders wich occur in this condition. The aim of the trial was to study the nature of biochemical changes in blood plasma during the course of experimental PE. Materials and methods. The study was carried out on 36 sexually mature, outbred, white rats of both sexes, weighing 180-200 g, which were kept on a standard vivarium diet. Experimental papain PE in white rats was induced by a single intratracheal administration of 0.5 ml of papain solution (Merck KGaA, Germany) at a dose of 100 mg / kg body weight under light ether anesthesia. The rats were withdrawn from the experiment by decapitation under light ether anesthesia 10, 20, 30, 50 and 60 days after the onset of the disease. Biochemical studies of blood plasma - the content of alanine aminotransferase (ALT), aspartate aminotransferase (AST), protein, urea, creatinine, total bilirubin, glucose were carried out using automatic biochemical analyzer Selectra Pro-M (Netherlands). Results. It was found that 10 days after the onset of the disease, the levels of ALT and AST significantly decreased by 37%. The trend towards a decrease in ALT persisted after 20, 30 and 60 days; AST � after 20, 30, 50 and 60 days. It was found that the blood plasma protein significantly decreased 10 and 20 days after the onset of the disease by 33% and 38%, respectively. At the same time, the urea also decreased after 10 days by 36% and by 32% after 20 days. A similar trend of changes was found when studying the level of creatinine in blood plasma. After 10 and 20 days, creatinine decreased by 36% and 37%, respectively. As for total bilirubin, its blood plasma contentration increased by 68% after 10 days, and by 38% after 20 days. There were no changes in plasma glucose levels 10, 20, 30, 50 and 60 days after the onset of the disease. Conclusions. Experimental PE is characterized by the most pronounced changes in blood plasma biochemical parameters 10 and 20 days after the onset of the disease, which is manifested by a decrease of aminotransferases � ALT and AST, a decrease in total protein, urea, creatinine, and an increase of total bilirubin. Key words: experimental pulmonary emphysema, alanine aminotransferase, aspartate aminotransferase, protein, urea, creatinine, bilirubin, glucose. U

scholarly journals THE IMPACT OF POWDERS AND OIL ADDITIVES OF CINNAMON AND CLOVE IN QUAILS DIET AS ANTISTRESSOR AND ANTIOXIDANT DURING HOT MONTHS

2020 ◽  
Vol 51 (3) ◽  
pp. 760-766
Author(s):  
Mustafa & Wasman
Keyword(s):  
Heat Stress ◽  
Blood Plasma ◽  
Control Group ◽  
Standard Diet ◽  
Clove Oil ◽  
Cinnamon Oil ◽  
Oil Additives ◽  
The World ◽  
The Impact ◽  
In Blood Plasma

The present investigation was carried out to compare the impact of cinnamon and clove powders and their oil additives as anti-stressors on quails under heat stress which is one of the riskiest environmental conditions that influence poultry all over the world in general and Iraq in particular. For this purpose, 420 quails (5 weeks old) were chosen and randomly grouped into seven treatments with different treatments (T0: control (standard diet), T1: 2 g clove powder/kg diet, T2: 1 ml clove oil/kg diet, T3: 2 g cinnamon powder, T4: 1 ml cinnamon oil/kg diet, T5: 1 g clove powder+1 g cinnamon powder/kg diet, T6: 0.5 ml clove oil+0.5 ml cinnamon oil/kg diet) added to standard diet. The quails were fed with these diets for 17 weeks. The results indicated that adding clove, cinnamon, and their oils to quail diet under heat stress led to significantly (p<0.05) lower relative density of heat shock protein HSP40, HSP70, HSP90, the concentrations of (corticosterone hormone, MDA and CK oxidative stimulator enzymes in blood plasma) and heterophil-to-lymphocyte (H/L) ratio, however, the concentrations of antioxidant enzymes AOA, GPx, SOD and CAT in blood plasma were significantly (p<0.05) higher in all additive treatments compared with the control group. It was also seen that treatment with the oil additives resulted in higher improvement than the powder additives.

scholarly journals Predictive value of the aspartate aminotransferase to platelet ratio index and aspartate aminotransferase to alanine aminotransferase ratio in early diagnosis of intrahepatic cholestasis in pregnancy

2021 ◽  
Vol 8 (11) ◽  
pp. 650-654
Author(s):  
İbrahim Kale
Keyword(s):  
Platelet Count ◽  
Intrahepatic Cholestasis ◽  
Predictive Value ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
First Trimester ◽  
Control Group ◽  
Third Trimester ◽  
Apri Score ◽  
In Pregnancy

Objective: We aimed to investigate the predictive value of the first-trimester aspartate aminotransferase/platelet count ratio index (APRI) and aspartate aminotransferase/alanine aminotransferase ratio for intrahepatic cholestasis in pregnancy (ICP). Material and Methods: The clinical data of patients who admitted to the Obstetrics Department of Umraniye Training and Research Hospital, between 2015-2020 were analyzed retrospectively. The study group consisted of 44 patients with ICP and the control group consisted of randomly selected 92 healthy pregnant women. Results: The two groups were similar in terms of age, BMI, first and third-trimester platelet count and third-trimester hemoglobin level. Patients with ICP had a significantly higher first-trimester APRI and a lower first trimester AST/ALT ratio than the healthy controls (p <0.001, p = 0.001, respectively). According to the ROC analysis, the optimal cut-off value of the APRI to predict ICP was 0.191, with the sensitivity of 0.66 and specificity of 0.66 (AUC: 0,727), and the optimal cut-off value for AST/ALT ratio was 1.07, with the sensitivity of 0.64, and specificity of 0.62 (AUC: 0,681). Conclusion: The first-trimester APRI score and AST/ALT ratio is an easy, inexpensive, and non-invasive tool that may be useful in predicting ICP early.

scholarly journals Great Obstetrical Syndromes In G20210a Mutation Carriers Associated With High Prothrombin Activity

2020 ◽  
Author(s):  
Mariya Nikolaeva ◽  
Andrey Pavlovich Momot ◽  
Marina Sabirovna Zainulina ◽  
Natalia Nikolaevna Yasafova ◽  
Irina Alekseevna Taranenko
Keyword(s):  
Blood Plasma ◽  
Venous Blood ◽  
Threshold Value ◽  
Main Group ◽  
Trophoblast Invasion ◽  
Control Group ◽  
G20210a Mutation ◽  
Factor Ii ◽  
Invasion Waves ◽  
In Blood Plasma

Abstract Objective: to study the association between high activity of Factor II (prothrombin) in blood plasma with G20210A mutation and the development of great obstetrical syndromes.Material and methods: A prospective clinical cohort study was conducted on 290 pregnant women (average age 31.7±4.7 years old). The main group was made up of 140 G20210A patients, while the control group comprised 150 women with the wild G20210G type. The aim was to evaluate the activity of Factor II in the venous blood plasma during the stages of pregnancy with regard to trophoblast invasion waves. As per results, association analysis of Factor II activity value and gestational complications was carried out.Results: In the control group, the median (Me) of Factor II activity ranged from 108% (preconception period) to 144% (pregnancy) [95% CI 130-150]. In patients with the GA type, the value was significantly higher in related periods, ranging from 149% to 181% [95% CI 142-195], p<0.0001. With Factor II activity ranging from 148.5% to 180.6%, pregnancies in the main group had no complications. Higher levels of Factor II activity were associated with the development of early and/or severe preeclampsia (PE) and fetal growth retardation (FGR).Conclusion: The data obtained regarding Factor II activity in blood plasma, juxtaposed with the development of great obstetrical syndromes, allow to assume that manifestation of G20210A in early and/or severe PE and FGR is associated with this coagulation factor's level of activity. Threshold value of the Factor II activity with G20210A mutation, allowing to predict the development of PE, comprised 171.0% at the preconception stage (AUC – 0.86; p<0.0001) and within 7-8 weeks of gestation it was 181.3% (AUC – 0.84; p<0.0001).

scholarly journals Impact of long-term supplementation of zinc and selenium on their content in blood and hair in goats

2011 ◽  
Vol 56 (No. 2) ◽  
pp. 63-74 ◽  
Author(s):  
L. Pavlata ◽  
M. Chomat ◽  
A. Pechova ◽  
L. Misurova ◽  
R. Dvorak
Keyword(s):  
Blood Plasma ◽  
Whole Blood ◽  
Average Concentration ◽  
The Other ◽  
Control Group ◽  
Selenium Yeast ◽  
Hair Samples ◽  
The Impact ◽  
Se Status

This paper evaluates the impact of long-term supplementation of different forms of zinc (Zn) and selenium (Se) on the content of these substances in the blood and hair of goats. Two analogous supplementation experiments were performed. 37 goats divided into four groups were used in the first trial with the Zn supplementation. Group A (n = 10) was a control group (with no Zn administered). A further three groups (B, C, D) were supplemented with Zn in various forms. Group B (n = 9) with zinc oxide, Group C (n = 9) with zinc lactate and Group D (n = 9) with zinc chelate. The second trial with Se supplementation was carried out on 20 goats divided into four groups. Group E (n = 5) was a control group. The other three groups were administered Se. Group F (n = 5) was supplied with a selenium lactate-protein complex, Group G (n = 5) with sodium selenite and Group H (n = 5) with selenium yeast. Three months later blood and hair samples were taken from all animals and Zn and Se concentrations were determined in whole blood, plasma, and hair. Glutathione peroxidase (GSH-Px) activity was determined in the Se supplementation trial group. At the end of the trial the Zn concentrations in plasma and whole blood were without major differences between the groups. The plasma concentration of Zn did not increase from the initial value at the start of the trial. In hair the average concentration of Zn was 95.2&ndash;100.0 mg/kg<br />in all groups. No conclusive relation was confirmed between the values of Zn in hair and its concentration in blood. The Se concentration in whole blood (&micro;g/l) at the end of trial in supplemented groups (F &ndash; 188.8 &plusmn; 24.6; G &ndash; 197.2 &plusmn; 10.9; H &ndash; 190.1 &plusmn; 26.3) was significantly higher (P &lt; 0.01) than in the control group (E &ndash; 103.1 &plusmn; 23.5). Similarly, the activity of GSH-Px (&micro;kat/l) was significantly higher in all supplemented groups (F &ndash; 872.3 &plusmn; 94.8; G &ndash; 659.5 &plusmn; 176.4; H &ndash; 839.8 &plusmn; 150.8) than in the control group (E &ndash; 379.1 &plusmn; 63.5). Se content in hair (&micro;g/kg) was higher also in all trial groups (F &ndash; 242.3 &plusmn; 41.5; G &ndash; 200.5 &plusmn; 46.9; H &ndash; 270.0 &plusmn; 106.8) than in the control group (E &ndash; 174.7 &plusmn; 38.0). However, it was significantly (P &lt; 0.05) higher only in Group F. A conclusive correlation was identified between the Se concentration in whole blood and its content in hair (r = 0.54; P &lt; 0.05; n = 20). Based on the results it can be concluded that none of the supplemented forms of Zn increased its concentration in blood, plasma and hair. On the other hand, the administration of Se led to an increase in the Se concentration in blood, increased the activity of GSH-Px in whole blood and the Se content in hair. Based on the proven correlation and regression relation between the Se concentration in blood and its content in hair, hair can be considered as a suitable material for the diagnosis of long-term Se status in goats. Goats with sufficient Se status are those that have more than 160 &micro;g/kg of Se in hair dry weight.

scholarly journals Overexpression of miRNA-21 Promotes the Proliferation and Invasion in Hepatocellular Carcinoma Cells via Suppressing SMAD7

2019 ◽  
Vol 18 ◽  
pp. 153303381987868 ◽  
Author(s):  
Yan Wang ◽  
Ping Zhang ◽  
Mei Yuan ◽  
Xiaojie Li
Keyword(s):  
Hepatocellular Carcinoma ◽  
Family Member ◽  
Reporter Gene ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Hepatoma Cell ◽  
Luciferase Reporter ◽  
Control Group ◽  
Luciferase Reporter Gene ◽  
Proliferation And Invasion

Purpose: This study aimed to explore the molecular mechanism of microRNA-21 and smad family member 7 in hepatocellular carcinoma. Method: A total of 57 participants were divided into control group (healthy participants, n = 10) and hepatocellular carcinoma group (hepatocellular carcinoma patients, n = 37). The expression of microRNA-21 levels were first detected in these two groups. Cell transfection was performed on hepatoma cell lines, followed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and Transwell assay to reveal proliferation and invasion ability. Furthermore, the relation between microRNA-21 and smad family member 7 was revealed by luciferase reporter gene and RNA immunoprecipitation assay. Finally, a transplantation tumor model of breast cancer in mice was constructed. Results: The serum indicators including α-alanine aminotransferase, aspartate aminotransferase, and albumin were differentially expressed between hepatocellular carcinoma group and control group. Compared to the control group, there was a high expression of microRNA-21 in hepatocellular carcinoma group. Low expression of microRNA-21 inhibited the proliferation and invasion of HepG2.2.15 and Huh7-1.3 cells. Luciferase reporter gene and RNA innumoprecipitation assay showed that smad family member 7 was the target gene of microRNA-21. Moreover, mice model analysis showed that microRNA-21 might regulate the growth of the transplanted tumors in mice by targeting smad family member 7. Conclusion: The upregulated microRNA-21 might participate in the proliferation and migration in cells of hepatocellular carcinoma via suppression of smad family member 7. Furthermore, serum indicators such as alanine aminotransferase, aspartate aminotransferase, and albumin might be used as serum diagnostic markers for hepatocellular carcinoma.

Trace Element Status (Zinc, Copper, Selenium, Iron, Manganese) in Patients with Long-Term Home Parenteral Nutrition

2016 ◽  
Vol 69 (2) ◽  
pp. 120-124 ◽  
Author(s):  
Milan Dastych Jr. ◽  
Michal Šenkyřík ◽  
Milan Dastych ◽  
František Novák ◽  
Petr Wohl ◽  
...  
Keyword(s):  
Trace Elements ◽  
Parenteral Nutrition ◽  
Blood Plasma ◽  
Whole Blood ◽  
Statistical Significance ◽  
Home Parenteral Nutrition ◽  
Control Group ◽  
The Status ◽  
In Blood Plasma

Background: The objective of the present study was to determine concentrations of zinc (Zn), copper (Cu), iron (Fe), selenium (Se) in blood plasma and manganese (Mn) in the whole blood in patients with long-term home parenteral nutrition (HPN) in comparison to the control group. Patients and Methods: We examined 68 patients (16 men and 52 women) aged from 28 to 68 years on a long-term HPN lasting from 4 to 96 months. The short bowel syndrome was an indication for HPN. The daily doses of Zn, Cu, Fe, Se and Mn in the last 3 months were determined. Results: No significant differences in blood plasma were found for Zn, Cu and Fe in patients with HPN and in the control group (p > 0.05). The concentration of Mn in whole blood was significantly increased in HPN patients (p < 0.0001), while Se concentration in these patients was significantly decreased (p < 0.005). The concentration of Mn in the whole blood of 16 patients with cholestasis was significantly increased compared to the patients without cholestasis (p < 0.001). The Cu concentration was increased with no statistical significance. Conclusion: In long-term HPN, the status of trace elements in the patients has to be continually monitored and the daily substitution doses of these elements have to be flexibly adjusted. Dosing schedule needs to be adjusted especially in cases of cholestatic hepatopathy. A discussion about the optimal daily dose of Mn in patients on HPN is appropriate. For clinical practice, the availability of a substitution mixture of trace elements lacking Mn would be advantageous.

scholarly journals β-Carotene Oxygenase 1 Activity Modulates Circulating Cholesterol Concentrations in Mice and Humans

2020 ◽  
Vol 150 (8) ◽  
pp. 2023-2030 ◽  
Author(s):  
Jaume Amengual ◽  
Johana Coronel ◽  
Courtney Marques ◽  
Celia Aradillas-García ◽  
Juan Manuel Vargas Morales ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Vitamin A ◽  
Total Cholesterol ◽  
Plasma Cholesterol ◽  
Hdl Cholesterol ◽  
Cholesterol Concentration ◽  
Atherosclerotic Cardiovascular Disease ◽  
Wild Type ◽  
The Impact ◽  
Β Carotene

ABSTRACT Background Plasma cholesterol is one of the strongest risk factors associated with the development of atherosclerotic cardiovascular disease (ASCVD) and myocardial infarction. Human studies suggest that elevated plasma β-carotene is associated with reductions in circulating cholesterol and the risk of myocardial infarction. The molecular mechanisms underlying these observations are unknown. Objective The objective of this study was to determine the impact of dietary β-carotene and the activity of β-carotene oxygenase 1 (BCO1), which is the enzyme responsible for the conversion of β-carotene to vitamin A, on circulating cholesterol concentration. Methods In our preclinical study, we compared the effects of a 10-d intervention with a diet containing 50 mg/kg of β-carotene on plasma cholesterol in 5-wk-old male and female C57 Black 6 wild-type and congenic BCO1-deficient mice. In our clinical study, we aimed to determine whether 5 common small nucleotide polymorphisms located in the BCO1 locus affected serum cholesterol concentrations in a population of young Mexican adults from the Universities of San Luis Potosí and Illinois: A Multidisciplinary Investigation on Genetics, Obesity, and Social-Environment (UP AMIGOS) cohort. Results Upon β-carotene feeding, Bco1−/− mice accumulated &gt;20-fold greater plasma β-carotene and had ∼30 mg/dL increased circulating total cholesterol (P &lt; 0.01) and non–HDL cholesterol (P &lt; 0.01) than wild-type congenic mice. Our results in the UP AMIGOS cohort show that the rs6564851 allele of BCO1, which has been linked to BCO1 enzymatic activity, was associated with a reduction in 10 mg/dL total cholesterol concentrations (P = 0.009) when adjusted for vitamin A and carotenoid intakes. Non–HDL-cholesterol concentration was also reduced by 10 mg/dL when the data were adjusted for vitamin A and total carotenoid intakes (P = 0.002), or vitamin A and β-carotene intakes (P = 0.002). Conclusions Overall, our results in mice and young adults show that BCO1 activity impacts circulating cholesterol concentration, linking vitamin A formation with the risk of developing ASCVD.

scholarly journals Tagging juvenile European perch (Perca fluviatilis L.) with passive integrated transponders (PIT) – impact on growth, condition, and physiological indexes

2017 ◽  
Vol 25 (3) ◽  
pp. 201-207 ◽  
Author(s):  
Zdzisław Zakęś ◽  
Maciej Rożyński ◽  
Elżbieta Ziomek ◽  
Krystyna Demska-Zakęś
Keyword(s):  
Blood Plasma ◽  
Growth Condition ◽  
Negative Impact ◽  
Fish Growth ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Feed Conversion ◽  
European Perch ◽  
Passive Integrated Transponders ◽  
The Impact

Abstract The aim of the study was to determine the impact of intramuscular tagging with passive integrated transponders (PIT tags) on the basic condition and hematological and biochemical indexes of the blood plasma of juvenile European perch (initial body weight of approximately 80 g). Throughout the observation period, i.e., for 42 days following PIT implantation, the procedure was not noted to have had a negative impact on fish growth, condition, or feed conversion ratio. The blood plasma biochemical indicators analyzed did not differ statistically significantly between the fish tagged with PIT and the untagged control group. No significant impact from this procedure was noted in the hematological indexes tested. Only with regard to the mean corpuscular hemoglobin (MCH) parameter was the value significantly lower in the fish tagged with PIT than in the control group (P ≤ 0.05). The difference determined was 1.40 pg (28.78 vs. 30.18). The MCH level in the fish tagged with PIT most probably was within the norm for this species. High tag retention (100%) and the lack of any significant impact on condition or hematological and biochemical indexes (with the exception of MCH) permits recommending this tagging method for use in juvenile perch.

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