scholarly journals PECULIARITIES OF BLOOD BIOCHEMICAL DISORDERS IN EXPERIMENTAL PULMONARY EMPHYSEMA

2021 ◽  
Vol 29 (2) ◽  
pp. 69-71
Author(s):  
V. I. Korzhov ◽  
◽  
B. O. Osipenko ◽  
M. P. Budennaya ◽  
M. G. Palivoda
Keyword(s):  
Blood Plasma ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Pulmonary Emphysema ◽  
Total Bilirubin ◽  
Experimental Studies ◽  
Ether Anesthesia ◽  
Intratracheal Administration ◽  
White Rats ◽  
In Blood Plasma

Experimental studies are among the important and promising approaches in the studying of the pathogenesis of pulmonary emphysema (PE). They make it possible to trace and understand the sequence of metabolic disorders wich occur in this condition. The aim of the trial was to study the nature of biochemical changes in blood plasma during the course of experimental PE. Materials and methods. The study was carried out on 36 sexually mature, outbred, white rats of both sexes, weighing 180-200 g, which were kept on a standard vivarium diet. Experimental papain PE in white rats was induced by a single intratracheal administration of 0.5 ml of papain solution (Merck KGaA, Germany) at a dose of 100 mg / kg body weight under light ether anesthesia. The rats were withdrawn from the experiment by decapitation under light ether anesthesia 10, 20, 30, 50 and 60 days after the onset of the disease. Biochemical studies of blood plasma - the content of alanine aminotransferase (ALT), aspartate aminotransferase (AST), protein, urea, creatinine, total bilirubin, glucose were carried out using automatic biochemical analyzer Selectra Pro-M (Netherlands). Results. It was found that 10 days after the onset of the disease, the levels of ALT and AST significantly decreased by 37%. The trend towards a decrease in ALT persisted after 20, 30 and 60 days; AST � after 20, 30, 50 and 60 days. It was found that the blood plasma protein significantly decreased 10 and 20 days after the onset of the disease by 33% and 38%, respectively. At the same time, the urea also decreased after 10 days by 36% and by 32% after 20 days. A similar trend of changes was found when studying the level of creatinine in blood plasma. After 10 and 20 days, creatinine decreased by 36% and 37%, respectively. As for total bilirubin, its blood plasma contentration increased by 68% after 10 days, and by 38% after 20 days. There were no changes in plasma glucose levels 10, 20, 30, 50 and 60 days after the onset of the disease. Conclusions. Experimental PE is characterized by the most pronounced changes in blood plasma biochemical parameters 10 and 20 days after the onset of the disease, which is manifested by a decrease of aminotransferases � ALT and AST, a decrease in total protein, urea, creatinine, and an increase of total bilirubin. Key words: experimental pulmonary emphysema, alanine aminotransferase, aspartate aminotransferase, protein, urea, creatinine, bilirubin, glucose. U

scholarly journals Application infusion drugs based on sorbitol for normalization of aminotransferase activity blood cancer patients operated cardia of the stomach

2020 ◽  
pp. 29-30
Author(s):  
B.R. Dzis ◽  
S.V. Prymak ◽  
R.P. Dzis ◽  
V.L. Novak ◽  
M.P. Dzisiv ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Postoperative Period ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Proximal Gastrectomy ◽  
Early Postoperative Period ◽  
Aminotransferase Activity ◽  
Cardiac Cancer ◽  
Intravenous Infusions ◽  
In Blood Plasma

Objective. To study the effect of intravenous infusions of Rheosorbilact on the activity of alanine aminotransferase and aspartate aminotransferase in the plasma of operated patients with gastric cardiac cancer after proximal gastrectomy. Materials and methods. Alanine aminotransferase and aspartate aminotransferase activity levels were studied in plasma of 40 operated patients with gastric cardiac cancer after proximal gastrectomy immediately after surgery, on the 1st, 2nd, 3rd, 5th day after intravenous infusions of the drug. The infusion of Rheosorbilact was administered intravenously, drip, for 5 days at a rate of 40 drops per minute. The daily dose of the drug was 1000.0 ml. Results. In patients with gastric cardiac cancer after proximal gastrectomy in the first days after surgery revealed changes in aminotransferases, which are accompanied by an increase in the activity of alanine aminotransferase and aspartate aminotransferase in blood plasma. To correct the activity of plasma aminotransferases in such patients, Rheosorbilact was administered intravenously immediately after surgery. After repeated intravenous infusions of Rheosorbilact for 5 days, a significant decrease in the activity of alanine aminotransferase and aspartate aminotransferase in blood plasma in the operated patients was revealed. Conclusions. In the first days after proximal gastrectomy in patients with gastric cardiac cancer, an increase in the activity of aminotransferases in blood plasma was revealed. Repeated intravenous infusions of Rheosorbilact normalize the activity of alanine aminotransferase and aspartate aminotransferase in the blood plasma of such patients in the early postoperative period. Infusion drug Rheosorbilact is recommended for widespread medical use in patients with cardiac gastric cancer after proximal gastrectomy, especially in the early postoperative period.

scholarly journals Zinc as a feed supplement and its impact on plasma cholesterol concentrations in breeding cocks

2011 ◽  
Vol 80 (3) ◽  
pp. 281-285 ◽  
Author(s):  
Tomáš Parák ◽  
Eva Straková
Keyword(s):  
Blood Plasma ◽  
Total Cholesterol ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Plasma Cholesterol ◽  
Control Group ◽  
Total Proteins ◽  
Zinc Compounds ◽  
The Impact ◽  
In Blood Plasma

The aim of this work was to verify the impact of feed supplemented with selected inorganic and organic zinc compounds on the total cholesterol concentrations and other blood plasma indices in breeding cocks. A total of 250 RIR 05 breeding cocks, 9 weeks old, were used. The cocks were divided into 5 groups of 50 animals each (four experimental groups and one control group). Cocks were fed a commercial feed mixture specifically for breeding cocks, containing 30.4 mg·kg-1 of zinc. The feed for experimental groups of cocks was fortified to 100 mg Zn·kg-1: zinc sulphate in first group, zinc oxide in second group, fodder yeast (Minvital Zn) in third group and Bioplex Zn in fourth group. Blood samples for biochemical examination were taken from the basilic vein. The contents of total cholesterol (Chol), total proteins (TP), glucose (Glu), aspartate aminotransferase (AST), alanine aminotransferase (ALT), calcium (Ca), phosphorus (P) and magnesium (Mg) in blood plasma were spectrophotometrically measured using a biochemical analyser, Cobas EMira, and commercial kits (Biovendor a.s., Czech Republic). In the 15th week of age, third and fourth groups showed a significant (P ≤ 0.05) and highly significant (P ≤ 0.01) decrease of total cholesterol in blood plasma compared to the control. In the 20th and 25th week of age, all of the experimental groups showed a significantand highly significant (P ≤ 0.01) decrease of total cholesterol in blood plasma compared to the control. Other monitored indices (total proteins, glucoses, aspartate aminotransferase, alanine aminotransferase, calcium, phosphorus and magnesium) did not reveal any significant changes between the experimental and the control groups. The presented work provides the first available experimental evidence regarding the impact of zinc supplementation on the cholesterol levels in blood plasma of breeding cocks.

Explore the mechanism of Swertia mussotii Franch. for hepatoprotective effects with iTRAQ-LC-MS/MS

2020 ◽  
Vol 23 ◽  
Author(s):  
Haixia Yun ◽  
Xinyu Wu ◽  
Yiwei Ding ◽  
Wendou Xiong ◽  
Xianglan Duan ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Total Bilirubin ◽  
Acute Liver Injury ◽  
Proteome Analysis ◽  
Ppi Networks ◽  
Hepatoprotective Effects

Background and Objective : A Tibetan traditional herb named Swertia mussotii Franch., also called “Zangyinchen” by the local people of Qinghai-Tibet area, has been used to protect the liver from injury for many years. However, the curative effect and molecular mechanism of the herb have not been demonstrated clearly. Materials and Methods: In our study, serum alanine aminotransferase, aspartate aminotransferase, total bilirubin levels were examined after S. mussotii Franch. treatment in the acute liver injury of the carbon tetrachloride-induced rat model. Then, Proteome Analysis was applied to explore the potential mechanism of SMT for hepatoprotective effects after iTRAQLC-MS/MS analysis (isobaric tag for relative and absolute quantification-liquid chromatograph-mass spectrometer with tandem mass spectrometry). Results: Serum results showed, alanine aminotransferase, aspartate aminotransferase, total bilirubin levels of rats with acute liver injury were all improved with SMT treatment. Moreover, Proteome Analysis suggested that, with S. Mussotii Franch. treatment, the levels of lipid catabolic process and lipid homeostasis were all enhanced. And the results of protein-protein interaction (PPI) analysis illustrated that these proteins assembled in PPI networks were found almost significantly enriched in response to lipid, negative regulation of lipase activity, response to lipopolysaccharide etc. Furthermore, the downregulated MRP14 and MRP8 proteins were found involved in the lipid metabolism, which may indicate the mechanism of SMT protection liver from ALI induced by carbon tetrachloride. Conclusion: SMT herb could play a role in hepatoprotection and alleviate the effect of acute liver injury by impacting the lipid metabolism associated biological process.

scholarly journals EVALUATION OF TOXIC EFFECTS OF MAGNETIC CONTRAST DIAGNOSTIC GADOLINIUM-CONTAINING NANOCOMPOSITE

2019 ◽  
Vol 98 (10) ◽  
pp. 1161-1165
Author(s):  
Larisa M. Sosedova ◽  
E. A. Titov ◽  
M. A. Novikov ◽  
V. A. Vokina ◽  
V. S. Rukavishnikov
Keyword(s):  
Nervous Tissue ◽  
Chemical Elements ◽  
Biological Effects ◽  
Experimental Studies ◽  
Biological Response ◽  
Immunohistochemical Method ◽  
Ether Anesthesia ◽  
Compensatory Response ◽  
White Rats ◽  
Liver And Kidney

Introduction. In recent years, magnetic nanoparticles, which can simultaneously have a therapeutic effect on the pathological focus, are used to magnify contrast enhancement and increase diagnostic sensitivity during magnetic resonance therapy (MRT). The last is carried out by the effective capture of neutrons, which among all the chemical elements is most pronounced in gadolinium. The use of gadolinium nanoparticles encapsulated in a polymeric matrix allows increasing the bioavailability of nanoparticles, reduces the possible toxicity of drugs. Aim. Evaluation of impact of new nanocomposite magnetically active metal complex gadolinium system on the morphofunctional state of the nervous tissue, liver, and kidney of rats. Material and methods. Experimental studies of biological effects of gadolinium-arabinogalactan nanocomposite (Gd-AG) were carried out on rats that were injected intraperitoneally for 10 days at the dose of 500 μg/kg in 0.5 ml of saline. Animals were sacrificed by decapitation under light ether anesthesia the next day after the end of exposure. To perform pathological studies, frontal sections of the temporal-parietal zone of the sensorimotor cortex, liver and kidney tissues were stained on ordinary histological glass slides with hematoxylin and eosin for viewing microscopic picture. The immunohistochemical method was used to determine the activity of the bcl-2, caspase-3 and hsp70 modulatory protein in apoptosis of white rats in brain neurons and to study the biological response of the organism at the subcellular level. Results. Histological analysis of tissues revealed a pronounced compensatory response of liver, a violation of the functional activity of kidneys. A decrease in the total number of normal neurons per unit area in brain tissue and an increase in the number of acts of neuronophagy indicate the initial stage of neurodegenerative process. Evaluation of the intracellular metabolism of neurons has not established the presence of signs characteristic of apoptotic process. Conclusion. The subacute effect of Gd-AG in a dose of 500 µg/kg causes a disturbance of morphofunctional state of liver, kidneys and nervous tissue, as well as modulation of cellular proteomics.

scholarly journals Comparison of a full-spectrum multi-analyte clinical analyser with six reference instruments using canine and feline blood samples

2017 ◽  
Vol 62 (No. 6) ◽  
pp. 342-350
Author(s):  
CS Lin ◽  
GH Chiang ◽  
CH Liu ◽  
HC Tsai ◽  
CC Yang ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Bile Acid ◽  
Blood Urea Nitrogen ◽  
Total Protein ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Total Bilirubin ◽  
Total Bile Acid ◽  
Analytical Performance ◽  
Blood Samples

In this study, we report the characterisation of a novel centrifugation and spectrum-integrated veterinary clinical analyser, the AmiShield<sup>TM</sup>, which has been developed for the multiplex measurement of biochemical, electrolyte and immunoassay parameters in a point-of-care testing environment. The aims of this study were to evaluate the analytical performance of the AmiShield<sup>TM</sup> and to compare it with six reference instruments using clinical blood samples. Two hundred and four canine and 120 feline blood samples collected from veterinary teaching hospitals were analysed in parallel using the AmiShield and appropriate reference instruments. All results were evaluated separately for canine and feline specimens. The instrument’s analytical performance was evaluated initially for short- and long-term precision, bias, and observed total error using quality control material. This was followed by comparison of clinical specimens on the AmiShield analyser in parallel with the Vitros and Hitachi for biochemical parameters, VetScan and SNAPshot for total bile acids, and VetLyte and Biolyte for electrolytes. Overall, the AmiShield analyser’s performance met the standards of the American Society for Veterinary Clinical Pathology for total allowable error for most analytes, and can be considered suitable for use in veterinary clinical practices. Using canine samples, excellent correlation coefficients (r ≧ 0.92) were identified for 14 analytes of various categories including glucose, total protein, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total bilirubin, amylase, blood urea nitrogen, creatinine, phosphorus, Na<sup>+</sup>, K<sup>+</sup>, Cl<sup>–</sup> and total bile acid, while good correlations (0.91 ≧ r ≧ 0.80) were recorded for albumin (r = 0.91). Bland-Altman difference plots also showed agreement (greater than 95% within Limits of Agreement) for glucose, total protein, albumin, alanine aminotransferase, alkaline phosphatase, total bilirubin, amylase, blood urea nitrogen, creatinine, Na<sup>+</sup>, K<sup>+</sup>, Cl<sup>–</sup> and total bile acid between AmiShield and the reference instruments. However, aspartate aminotransferase and phosphorus exhibited higher outliers, implying potential problems associated with matrix interferences such as lipemic samples, which warrant further study. This study demonstrates that the AmiShield compares favourably with standard reference instruments, and the new device generated data of high quality for most analytes in clinical canine and feline samples. The capability of reliably measuring multi-category analytes in one device using minute amounts (170 μl) of whole blood and short turn-around times (&lt; 15 min) underlines the high potential of the device as a good alternative in-house diagnostic application.

scholarly journals A Comparative Study on the Hepatoprotective Effect of Tamarindus indica and Vitamin E in Long Evans Rats

2014 ◽  
Vol 6 (2) ◽  
pp. 63-67 ◽  
Author(s):  
NJ Shammi ◽  
ZK Choudhry ◽  
MI Khan ◽  
MM Hossain
Keyword(s):  
Alkaline Phosphatase ◽  
Vitamin E ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Total Bilirubin ◽  
Histopathological Examination ◽  
Protective Effects ◽  
Tamarindus Indica ◽  
Ethanolic Extracts ◽  
Long Evans

The protective effects of ethanolic extract of Tamarindus indica leaves and seeds in compoarison to vitamin E, were studied on paracetamol induced hepatotoxicity in Long Evans Rats. Different groups of animals were administered in the paracetamol (1500mg /kg, p.o.) for 7 days. Ethanolic extracts of leaves and seeds of Tamarindus indica (1250mg/kg) with parallel vitamin E (500 mg/kg), were administered to paracetamol pretreated rats. On treatment with paracetamol a significant increase in alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin and alkaline phosphatase were observed. On administration of ethanolic extracts of leaves and seeds a significant decrease in the level of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin and alkaline phosphatase (ALP) were observed and histopathological examination of liver tissue revealed an almost return to normal architecture. The result were almost comparable to vitamin E, a known hepatoprotective agent. DOI: http://dx.doi.org/10.3329/bjmb.v6i2.17645 Bangladesh J Med Biochem 2013; 6(2): 63-67

scholarly journals Comparison of postoperative liver function between different dissection techniques during laparoscopic cholecystectomy

2020 ◽  
pp. FSO462
Author(s):  
Tagleb S Mazahreh ◽  
Abdelwahab J Aleshawi ◽  
Nabil A Al-Zoubi ◽  
Mohammad Altabari ◽  
Qusai Aljarrah
Keyword(s):  
Laparoscopic Cholecystectomy ◽  
Liver Function ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Total Bilirubin ◽  
Liver Function Tests ◽  
Different Types ◽  
Group A ◽  
Dissection Techniques ◽  
Group B

Aim: In this study, we investigated and compared the effect of different types of dissector (Maryland vs Hook) on changes in liver function tests (LFTs) after laparoscopic cholecystectomy. Patients & methods: The enrolled patients were divided into two groups. Group A patients underwent dissection by Maryland dissecting forceps, group B by Hook dissecting instrument. LFTs were measured preoperatively and at 1 day and 1 week, postoperatively. Results: For both Maryland and Hook dissection, the 1-day postoperative values for total bilirubin, alanine aminotransferase and aspartate aminotransferase were significantly higher than the preoperative values. Also, there were no statistical differences between Hook and Maryland. Conclusion: The elevation of LFTs seems to be attributed to other factors.

scholarly journals The safety of prolong usage of diazepam on liver and renal functions in rats

2021 ◽  
Vol 2 (1) ◽  
pp. 059-061
Author(s):  
Wajdy Jabbar Majid ◽  
Tayseer Ali Talab
Keyword(s):  
Alkaline Phosphatase ◽  
Body Weight ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Total Bilirubin ◽  
Intraperitoneal Administration ◽  
Blood Chemistry ◽  
Serum Glucose ◽  
Male Rats ◽  
Renal Functions

In this study the effect of diazepam on some blood chemistry values was studied in male rats. Intraperitoneal administration of diazepam at a dose of 0.6mg/kg body weight for 60 days didn't induce significant changes in serum glucose, urea, cholesterol, aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin, creatinine and alkaline phosphatase levels. Findings of this study give further sound for the safety of benzodiazepines.

scholarly journals Protein-synthesizing function and functional state of the liver of dogs at experimental toxocariasis

2020 ◽  
Vol 22 (98) ◽  
pp. 132-137
Author(s):  
W. S. Said ◽  
V. V. Stybel ◽  
B. V. Gutyj ◽  
O. B. Pryima ◽  
I. Y. Mazur
Keyword(s):  
Body Weight ◽  
Functional State ◽  
Total Protein ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Experimental Studies ◽  
Control Group ◽  
Activity Of Enzymes ◽  
Experimental Group

The aim of the study was to investigate the effect of toxocariasis invasion on protein-synthesizing function and functional state of the liver of dogs. For experimental studies, 12 dogs aged from two to four months were used and two groups of six animals were formed in each: control and experimental. Puppies of the experimental group were experimentally infected with the pathogen toxocariasis at a dose of 5,000 invasive eggs of T. canis per kg of body weight. The control puppies were clinically healthy. Protein-synthesizing function of the liver of dogs in experimental toxocariasis was studied by the level of total protein and its fractions. It was found that on the 25th day of the experiment the level of total protein in the blood of the experimental group of dogs was 56.9 ± 1.47 g/l, while in the control group – 63.8 ± 2.92 g/l. The lowest level of the studied indicator was on the 30th day of the experiment in the blood of the experimental group of dogs, where it decreased by 9.6 %. The results of a study of protein fractions in the blood of dogs infested with toxocara showed that the percentage of albumin is likely to decrease. However, in the blood of infected dogs in this period of research there was a probable increase in globulin levels. The study of the effect of toxocariasis invasion on the activity of enzymes in blood serum is a test for the morphological and functional state of tissues and organs. Based on the studies, it was found that with the development of toxocariasis invasion in dogs there is an increase in alanine aminotransferase in their serum. On the 25th and 30th day of the experiment, the activity of the enzyme in the serum of infected dogs was the highest, where compared with the control group of dogs, the activity of ALT increased by 51.2 and 59.1 %, respectively. Similar changes in activity were observed in the determination of aspartate aminotransferase, where, respectively, on the 15th, 20th and 25th day of the experiment, the activity of AST in the serum of infected dogs increased by 19.3, 27.5 and 39.1 %.

scholarly journals Influence of 8-Hour Road Transportation on Selected Physiological Parameters in Horses

2012 ◽  
Vol 56 (2) ◽  
pp. 193-197 ◽  
Author(s):  
Artur Niedźwiedź ◽  
Marcin Zawadzki ◽  
Henryk Filipowski ◽  
Józef Nicpoń
Keyword(s):  
Alkaline Phosphatase ◽  
Creatine Kinase ◽  
Total Protein ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Total Bilirubin ◽  
Physiological Responses ◽  
Venous Blood ◽  
Road Transportation ◽  
Physiological Variables

Abstract The objective of this study was to assess the physiological responses in mature healthy horses prior to an 8 h transport, as well as immediately and 24 h after transportation in a horse truck, under autumn conditions typical for central Poland. The study was conducted on a group of 60 horses of different breed. Horses ranged from 4 to 10 years of age. Venous blood was taken 3 times: I - directly before loading the horses to the trailer, II - directly after unloading the horses from the trailer, III - after period of 24 h resting. There was no change in the concentrations of urea, cholesterol, creatinine, and magnesium and in the activity of alkaline phosphatase during the three subsequent samplings. Albumin, total protein, alanine aminotransferase, aspartate aminotransferase, total bilirubin, creatine kinase, and triglycerides were mostly influenced by the transport. The obtained results indicate that the transportation of horses alters concentrations of physiological variables of their metabolism.

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