scholarly journals Improving bio-butanol production from lignocellulosic feedstock by tailoring metabolic perturbations

2021 ◽  
Author(s):  
Jin Kang
Keyword(s):  
Wheat Straw ◽  
Metabolic Flux ◽  
Treatment Method ◽  
Methyl Red ◽  
Butanol Production ◽  
Chemical Additives ◽  
Microbial Inhibitors ◽  
Lignocellulosic Feedstock ◽  
Wheat Straw Hydrolysate ◽  
Optimized Conditions

The objective of this study is to enhance bio-butanol production using lignocellulosic feedstock via supplements of metabolism perturbation. Metabolic perturbations are non-substrate-based chemical additives that can reinforce metabolic flux towards butanol formation, or increase tolerance to microbial inhibitors in the feedstock. Typical metabolic perturbations include CaCO3, ZnSO4, methyl red, and furan derivatives such as furfural and hydroxymethylfurfural (HMF). In this study, we stepwise tailored metabolic perturbations to maximize butanol production from pure sugar and lignocellulosic feedstock. Under optimized conditions of 4 g/L CaCO3, 2 mg/L ZnSO4, butanol production exceeded 10g/L in wheat straw hydrolysate, which was significantly higher than that obtained in the absent of ZnSO4 and CaCO3. As compared to traditional lignocellulosic feedstock post-treatment method, metabolic perturbations method shows advantages in terms of productivity and economics. Improved bio-butanol production is related to the overexpression of NAD(P)H dependent genes.

scholarly journals Improving bio-butanol production from lignocellulosic feedstock by tailoring metabolic perturbations

2021 ◽  
Author(s):  
Jin Kang
Keyword(s):  
Wheat Straw ◽  
Metabolic Flux ◽  
Treatment Method ◽  
Methyl Red ◽  
Butanol Production ◽  
Chemical Additives ◽  
Microbial Inhibitors ◽  
Lignocellulosic Feedstock ◽  
Wheat Straw Hydrolysate ◽  
Optimized Conditions

The objective of this study is to enhance bio-butanol production using lignocellulosic feedstock via supplements of metabolism perturbation. Metabolic perturbations are non-substrate-based chemical additives that can reinforce metabolic flux towards butanol formation, or increase tolerance to microbial inhibitors in the feedstock. Typical metabolic perturbations include CaCO3, ZnSO4, methyl red, and furan derivatives such as furfural and hydroxymethylfurfural (HMF). In this study, we stepwise tailored metabolic perturbations to maximize butanol production from pure sugar and lignocellulosic feedstock. Under optimized conditions of 4 g/L CaCO3, 2 mg/L ZnSO4, butanol production exceeded 10g/L in wheat straw hydrolysate, which was significantly higher than that obtained in the absent of ZnSO4 and CaCO3. As compared to traditional lignocellulosic feedstock post-treatment method, metabolic perturbations method shows advantages in terms of productivity and economics. Improved bio-butanol production is related to the overexpression of NAD(P)H dependent genes.

scholarly journals Simplification of urea treatment method of wheat straw for its better adoption by the farmers

2010 ◽  
Vol 39 (1) ◽  
Author(s):  
M.A Jabbar ◽  
H Muzafar ◽  
F.M Khattak ◽  
T.N Pasha ◽  
A Khalique
Keyword(s):  
Wheat Straw ◽  
Treatment Method ◽  
Urea Treatment

The effect of three strains of Pleurotus on the in vitro dry matter digestibility and subsequent nutritive value of wheat straw to ruminant animals

2005 ◽  
Vol 2005 ◽  
pp. 137-137
Author(s):  
E. M. Hodgson ◽  
M. D. Hale ◽  
H. M. Omed
Keyword(s):  
Wheat Straw ◽  
Nutritive Value ◽  
Animal Feed ◽  
White Rot Fungi ◽  
Wheat Plant ◽  
Treatment Method ◽  
White Rot ◽  
Dry Matter Digestibility ◽  
Ruminant Animals

Straw constitutes a vast, valuable, and under utilised agricultural by-product, which has a great potential for utilisation as an animal feedstuff. However, due to the way in which it is constructed, the digestible sugars, cellulose and hemicelluloses, are tightly chemically bound by heavily lignified cell walls which provide the wheat plant stem with its strength and structure, but in doing so greatly inhibit the digestibility and nutritive value of the material to ruminant animals. Therefore, the utilisation of this resource as an animal feed can only be realised effectively, if the nutritional and digestibility values of the material can be improved by the innovation and successful application of an effective treatment method, be that physical, chemical or biological. Previously devised methods of upgrading the digestibility and nutritive value of forages, with the possible exception of urea treatment, have proven either insufficient, environmentally unsound, or economically infeasible to those concerned, particularly those in developing world. Therefore, there is a distinct need to develop techniques which can avoid these pitfalls and still yield the desired results in the context of animal nutrition. Previous research has indicated that members of the genus Pleurotus white rot fungi, have great potential for application in the biological upgrading of wheat straw. Therefore, the objective of this work was to investigate biological techniques, using 3 strains of Pleurotus fungi which may have the potential to be utilised in the biological upgrading of wheat straw.

Detoxification of Wheat Straw Formic Acid Hydrolysis and Xylitol Production

2011 ◽  
Vol 383-390 ◽  
pp. 5453-5457 ◽  
Author(s):  
Jun Ping Zhuang ◽  
Lu Lin ◽  
Sun Yong ◽  
Chun Sheng Pang
Keyword(s):  
Ion Exchange ◽  
Formic Acid ◽  
Acid Hydrolysis ◽  
Wheat Straw ◽  
Exchange Resin ◽  
Reaction System ◽  
Ion Exchange Resin ◽  
Fermentation Inhibitors ◽  
Formic Acid Hydrolysis ◽  
Wheat Straw Hydrolysate

Xylitol can be obtained from hemicelullosic fraction of lignocellulosic materials containing D-xylose. Formic acid hydrolysis is widely used in lignocellulose pretreatment. However, formic acid hydrolysis wheat straw cannot be directly used as fermentation subsequently owing to various fermentation inhibitors, especially the formic acid in reaction system and released during pretreatment. This study describes main hydrolysis components and inhibitors prepared by hydrolysis of wheat straw with formic acid solution, and different detoxification methods ware used to romve the inhibitors. Study found treatment of wheat straw hydrolysate with overliming treatment and D311 ion-exchange resin had a good result on reduction formic acid and other main inhibitors, results showed which can eliminate 94% of residual formic acid and with only 15% reducion of xylose. To verify the effectiveness of different detoxification methods, hydrolysates of detoxification werr fermented by Candida.tropicalis AS2.1776, and the results also found that the hydrolysate treatmented with overliming treatment and D311 ion-exchange resin have the hightest xylitol yield.

scholarly journals Metabolic engineering of Aspergillus niger via ribonucleoprotein-based CRISPR–Cas9 system for succinic acid production from renewable biomass

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Lei Yang ◽  
Mikkel Møller Henriksen ◽  
Rasmus Syrach Hansen ◽  
Mette Lübeck ◽  
Jesper Vang ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Succinic Acid ◽  
Wheat Straw ◽  
Acid Production ◽  
Value Added ◽  
Fungal Cell ◽  
Renewable Biomass ◽  
Succinic Acid Production ◽  
Wheat Straw Hydrolysate ◽  
Low Culture

Abstract Background Succinic acid has great potential to be a new bio-based building block for deriving a number of value-added chemicals in industry. Bio-based succinic acid production from renewable biomass can provide a feasible approach to partially alleviate the dependence of global manufacturing on petroleum refinery. To improve the economics of biological processes, we attempted to explore possible solutions with a fungal cell platform. In this study, Aspergillus niger, a well-known industrial production organism for bio-based organic acids, was exploited for its potential for succinic acid production. Results With a ribonucleoprotein (RNP)-based CRISPR–Cas9 system, consecutive genetic manipulations were realized in engineering of the citric acid-producing strain A. niger ATCC 1015. Two genes involved in production of two byproducts, gluconic acid and oxalic acid, were disrupted. In addition, an efficient C4-dicarboxylate transporter and a soluble NADH-dependent fumarate reductase were overexpressed. The resulting strain SAP-3 produced 17 g/L succinic acid while there was no succinic acid detected at a measurable level in the wild-type strain using a synthetic substrate. Furthermore, two cultivation parameters, temperature and pH, were investigated for their effects on succinic acid production. The highest amount of succinic acid was obtained at 35 °C after 3 days, and low culture pH had inhibitory effects on succinic acid production. Two types of renewable biomass were explored as substrates for succinic acid production. After 6 days, the SAP-3 strain was capable of producing 23 g/L and 9 g/L succinic acid from sugar beet molasses and wheat straw hydrolysate, respectively. Conclusions In this study, we have successfully applied the RNP-based CRISPR–Cas9 system in genetic engineering of A. niger and significantly improved the succinic acid production in the engineered strain. The studies on cultivation parameters revealed the impacts of pH and temperature on succinic acid production and the future challenges in strain development. The feasibility of using renewable biomass for succinic acid production by A. niger has been demonstrated with molasses and wheat straw hydrolysate.

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