scholarly journals Pollen morphology and viability of some Indian mangroves

2008 ◽  
pp. 60-71 ◽  
Author(s):  
Suparna Gupta ◽  
Arunima Ghosh ◽  
Subrata Maity ◽  
Sauren Das
Keyword(s):  
Pollen Tube ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Tube Length ◽  
Strong Positive Correlation ◽  
Viable Pollen ◽  
The Family ◽  
Indian Mangroves ◽  
Pollen Tube Length

A study on pollen morphology of four common Indian mangroves from Sundarbans mangrove swamps, three species from the family Rhizophoraceae (Bruguiera sexangula Laur., Ceriops tagal Perr.and Rhizophora mucronata Lamk.) and one species from the family Avicenniaceae (Avicennia marina Forsk.) was carried out. Shape of the pollen grains were prolate, prolate spheroidal and subprolate with tricolporate apertures. Surface ornamentation of the studied taxa was reticulate, finely reticulate or scabrate. Pollen grain viability of the said taxa was determined by stain test (1% aceto-orcein) under in situ condition and percentage of viable pollen production was recorded. The result revealed that all the studied taxa produce a fairly good amount of viable pollen grains in natural condition, an indication that they are good propagule producer in the long run. Pollen grain germination and measurements of pollen tube length using different sucrose concentrations (0.2, 0.6, 1.0, 1.2, 1.6 and 2.0%). Maximum pollen fertility and pollen tube length occurred in 2% sucrose solution in case of A. marina and B. sexangula, whereas 1.6% in C. tagal and 1.0% in R. mucronata. A strong positive correlation exist between pollen germination and pollen tube length, but there was a hardly relation between sucrose concentration and pollen tube length.

scholarly journals Pollen viability in Quercus robur L.

2017 ◽  
Vol 69 (1) ◽  
pp. 111-117 ◽  
Author(s):  
Branislava Batos ◽  
Danijela Miljkovic
Keyword(s):  
Pollen Tube ◽  
Quercus Robur ◽  
Interindividual Variability ◽  
Germination Rate ◽  
Pollen Viability ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Tube Length ◽  
Pedunculate Oak ◽  
Pollen Tube Length

The variability of viability (germination rate and the length of pollen tubes) of fresh pedunculate oak (Quercus robur L.) pollen grains was studied in vitro on a medium containing 15% sucrose. Spatial variability was studied by sampling fresh pollen grains from a total of thirteen trees at four different sites in the area of Belgrade (Kosutnjak, Banovo Brdo, Ada Ciganlija and Bojcin Forest) in a single year (2011). In order to assess temporal variability and determine the effects of climate change on a small time scale, we studied the viability of the pollen grains collected from one tree at the Banovo Brdo site in six different years (2004, 2005, 2006, 2007, 2011 and 2012). Interindividual variability was tested on the pollen grains sampled from eight trees at Ada Ciganlija in 2004. The percentage values of the pollen grain germination rate and the pollen tube length showed no statistically significant differences between the sites. However, the studied characteristics of the pollen grain viability (germination rate and pollen tube length) showed statistically significant differences in both temporal (between the pollen collection years) and interindividual variability. This type of research makes a valuable contribution to pedunculate oak breeding programs through the identification of trees with stable production and a good quality of pollen. Furthermore, it can be important in defining the patterns of spatial, temporal and individual variability of pollen grain viability under the influence of climate factors, which are showing compelling changing trends from year to year.

scholarly journals Pollen characters as taxonomic evidence in some species of Dipsacaceae from Iran

2017 ◽  
Vol 24 (2) ◽  
pp. 129-136 ◽  
Author(s):  
Ebadi-Nahari Mostafa ◽  
Nikzat-Siahkolaee Sedigheh ◽  
Eftekharian Rosa
Keyword(s):  
Pollen Morphology ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Pollen Type ◽  
Upgma Tree ◽  
Pollen Characters ◽  
Exine Ornamentation ◽  
The Family ◽  
The World ◽  
Plant Taxon

Pollen morphology of nine species representing four genera: Cephalaria Schrad, Dipsacus L., Pterocephalus Vaill. and Scabiosa L. of the family Dipsacaceae in Iran has been investigated by means of scanning electron microscopy (SEM). The results showed that pollen grains were triporate and tricolpate. The pollen type of Scabiosa rotata Bieb. (tri- and tetraporate) is the first report in the world. The sizes of pollen grains fall into the classification group magna (pollen grain diameter 50–100 μm). Pollen shapes vary from preoblate to prolate and their polar views were triangulate and lobate. The exine ornamentation varies from gemmate in S. rotata to spinulate in the rest studied species. Species of Scabiosa have been dispersed in UPGMA tree that this confirmed the previous studies about taxonomic problems and species complexity in this genus. These results show the transfer of the some Scabisoa species to Lomelosia Raf. based on palynological characters. Pollen morphology of the family is helpful at the generic and specific level.Bangladesh J. Plant Taxon. 24(2): 129–136, 2017 (December)

scholarly journals Effects of storage temperature and duration on pollen grain viability and pollen-tube elongation in Chinese chinqapin (Castanea henryi Skan)

2020 ◽  
Vol 49 (2) ◽  
pp. 297-304
Author(s):  
Huan Xiong ◽  
Deyi Yuan ◽  
Zhi-Yu Deng ◽  
Genhua Niu ◽  
Feng Zou
Keyword(s):  
Pollen Tube ◽  
Room Temperature ◽  
Storage Temperature ◽  
Southern China ◽  
Germination Rate ◽  
Pollen Viability ◽  
Pollen Grain ◽  
Tube Length ◽  
Storage Duration ◽  
Pollen Tube Length

Chinese chinquapin [Castanea henryi (Skan) Rehder & E.H. Wilson] is used as a food and timber crop in southern China. Most chinquapin cultivars are self-incompatible and bloom at different times; consequently, artificial pollination is used to ensure fruit set and nut yield. Effective pollen storage that enables producers and breeders to use stored pollen for cross-pollination at a later date is important. In this study, the cultivar Changmangzi was used to estimate the viability and pollen tube length of pollen stored at room temperature, and at 4, −20, and −80°C using in vitro germination tests. It was observed that pollen grain germination significantly decreased at all four storage temperatures. Pollen viability was 14.4% after only 24 days of storage at room temperature. The germination rate was 13.3% after 90 days of storage at 4°C, and 14.5% after 180 days at −20°C. The initial germination rate of pollen stored at −80°C was 56.3% at the beginning of the test and decreased to 15.4% after 240 days. Pollen-tube length decreased with increased storage duration; mean pollen-tube lengths ranged from 109.44 to 257.51 μm. Based on these results, it is suggested that a storage temperature of −80°C for Changmangzi pollen is good.

Effect of nickel on pollen germination and pollen tube length in Arabis alpina (Brassicaceae)

2016 ◽  
Vol 64 (4) ◽  
pp. 302 ◽  
Author(s):  
Dolja Pavlova
Keyword(s):  
Pollen Tube ◽  
Pollen Germination ◽  
Pollen Grains ◽  
Tube Length ◽  
Serpentine Soils ◽  
Nutritional Medium ◽  
Arabis Alpina ◽  
Pollen Tube Elongation ◽  
Germinated Pollen ◽  
Pollen Tube Length

In this work we studied and compared the toxic effect of nickel (Ni) on pollen germination and pollen tube length in Arabis alpina L. collected from serpentine and non-serpentine populations distributed in the Rila mountains, Bulgaria. Pollen grains were treated with prepared standard solutions of 100, 300, 500, and 700 μM Ni as NiCl2 in distilled water. A nutritional medium was also used to assess pollen germination. Nickel inhibited pollen germination and pollen tube elongation in both serpentine and non-serpentine plants. The percentage of germinated pollen in serpentine plants treated with Ni was higher than in non-serpentine plants but there was no difference in pollen tube elongation between groups. However, pollen tubes showed abnormalities such as coiling and swelling of the tip, or burst, and varied considerably among the samples. A complete break of pollen tube elongation is due to such abnormalities. Also, decreased pollen fertility in both populations was found. The plants from serpentines were less sensitive to (i.e. more tolerant of) elevated Ni concentrations commonly found in serpentine soils.

In vitro germination and pollen tube growth of maize (Zea mays) pollen. II. Pollen source, calcium, and boron interactions

1968 ◽  
Vol 46 (3) ◽  
pp. 235-240 ◽  
Author(s):  
P. L. Pfahler
Keyword(s):  
Zea Mays ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Calcium Nitrate ◽  
Pollen Grains ◽  
Germination Percentage ◽  
Tube Growth ◽  
Tube Length ◽  
Pollen Tube Length

Pollen grains from six single cross hybrids (Wf9 × H55, Wf9 × H50, H49 × H55, H49 × H50, Ky49 × Ky27, and K64 × K55) of Zea mays L. were cultured on an artificial medium containing 15% sucrose and 0.6% bacto-agar supplemented with all possible combinations of two levels (0.00 and 0.03%) of calcium nitrate (Ca) and two levels (0.00 and 0.01%) of boric acid (B). The effect of Ca and B on germination percentage was found to be dependent on the genetic source of the pollen. At the 0.00% levels of Ca and B, the germination percentage of the hybrids ranged from 26.6% for Ky49 × Ky27 to 58.1% for K64 × K55. With either Ky49 × Ky27 or K64 × K55 pollen, the addition of Ca and B to the medium resulted in a lower germination percentage whereas the addition of Ca and B increased the germination percentage of the other hybrids. The addition of B in the absence of Ca sharply reduced the germination percentage in all hybrids while in the presence of Ca, the effect of B varied with the hybrid involved. For pollen tube length, highly significant interactions between hybrids and levels of Ca and B were found. However, maximum pollen tube lengths were obtained for all hybrids with both Ca and B in the medium. The addition of B in the absence of Ca reduced the pollen tube length of all hybrids whereas the addition of B in the presence of Ca increased pollen tube length. In general, the addition of both Ca and B increased the rate of pollen tube growth especially in the period 2 to 3 hours after inoculation.

IN VITRO GERMINATION AND POLLEN TUBE GROWTH OF MAIZE (ZEA MAYS L.) POLLEN.: I. CALCIUM AND BORON EFFECTS

1967 ◽  
Vol 45 (6) ◽  
pp. 839-845 ◽  
Author(s):  
P. L. Pfahler
Keyword(s):  
Pollen Tube ◽  
Boric Acid ◽  
Calcium Nitrate ◽  
Pollen Grains ◽  
Germination Percentage ◽  
Tube Length ◽  
Artificial Medium ◽  
Bacto Agar ◽  
Pollen Tube Length

Pollen grains from the single cross hybrid Wf9 × H55 were cultured on an artificial medium containing 15% sucrose and 0.6% bacto-agar supplemented with all possible combinations of three concentrations (0.00, 0.03, and 0.06%) of calcium nitrate and three concentrations (0.00, 0.01, and 0.02%) of boric acid. The combination containing 0.03% calcium nitrate and 0.01% boric acid produced the highest germination (47.9%) and the longest tube length (405 μ). The addition of either calcium nitrate or boric acid alone decreased the germination percentage and pollen tube length. The percentage of grains producing more than one pollen tube was reduced by the addition of calcium nitrate or boric acid singly or in combination. The significance of these results in relation to pollen physiology was discussed.

In vitro germination and pollen tube growth of maize (Zea mays) pollen. IV. Effects of the fertility restoring Rf1 locus

1971 ◽  
Vol 49 (1) ◽  
pp. 55-57 ◽  
Author(s):  
P. L. Pfahler
Keyword(s):  
Pollen Tube ◽  
Calcium Nitrate ◽  
Pollen Grains ◽  
Germination Percentage ◽  
Tube Length ◽  
In Vitro Germination ◽  
Germination Characteristics ◽  
Bacto Agar ◽  
Pollen Tube Length

Pollen grains from the near-isogenic inbreds, Oh 43 Rf1Rf1, Oh 43 rf1rf1, 106 Rf1Rf1, and 106 rf1rf1 were cultured on an artificial medium containing 15% sucrose and 0.6% bacto-agar supplemented with all possible combinations of two levels (0.00 and 0.03%) of calcium nitrate (Ca) and two levels (0.00 and 0.01%) of boric acid (B). At certain combinations of Ca and B, significant differences between the Rf1Rf1 and rf1rf1 genotypes were obtained for germination percentage, rupturing percentage, and pollen tube length. However, the effect of the Rf1 and rf1 alleles was not consistent in magnitude for both inbreds. Apparently, the Rf1 and rf1 alleles can alter certain in vitro germination characteristics but genetic background is also involved.

scholarly journals Morphological Study of Pollen Grains of Angiosperms

2019 ◽  
Vol 7 (3) ◽  
pp. 354-358
Author(s):  
Pushpa Karna Mallick
Keyword(s):  
Morphological Study ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Specific Level ◽  
Central Nepal ◽  
The Family ◽  
Euphorbia Milii ◽  
Pollination Systems ◽  
Plant Systematic

Pollen morphology of twelve species of angiosperms from Central Nepal was cytologically carried out in present investigation. In this investigation pollen morphology of Bignonia venusta Ker Gawl. and Jacranda mimisifolia D.Don from the family Bignoniaceae; Brassica compestris L. from Brassicaceae; Cuphea hyssopifolia Kunth. from Lythraceae; Coriandrum sativum L. from Apiaceae; Crepis japonica L. and Dichrocephala integrifolia (L f.) kunzte. from Asteraceae; Delphinium ajacis L. from Ranunculaceae; Dianthus barbetus L. from Caryophyllaceae; Euphorbia Milii Des Moul. and Euphorbia pulcherima L. from Euphorbiaceae and Magnolia fuscata from Magnoliaceae were studied. Shape of the pollen grains found to be mostly spheroidal, few circular, one elliptical, one triangular and one elongated in this study. Sculpture of exine wall found to be smooth, echidnae, tected, reticulated and perforated type. Aperture of the pollen grains found to be triporate to pentaporate. The large, medium and small sized pollen grains were observed in present research. The high diversty of sculpture type in pollen grains of angiosperms has been associated to diversity in pollination systems. Smooth pollen grains are linked with wind or water pollination while sculptured pollen grains associated with biotic pollination. The morphology of pollen grain is one of the significant tools in solving some taxonomic problems such as identification, tracing phylogenetic relationship on the family, generic or specific level and in plant systematic and evolution. Int. J. Appl. Sci. Biotechnol. Vol 7(3): 354-358

scholarly journals Effect of morphactin (IT 3456) on pollen germination and pollen tube growth in four species of Angiospermae

2014 ◽  
Vol 51 (2) ◽  
pp. 179-185
Author(s):  
Barbara Gabara
Keyword(s):  
Pollen Tube ◽  
Pollen Germination ◽  
Pollen Grains ◽  
Tube Growth ◽  
Tube Length ◽  
Antirrhinum Majus ◽  
Eschscholtzia Californica ◽  
Germination Process ◽  
Narcissus Poeticus ◽  
Pollen Tube Length

The action of morphactin in 10, 100 and 1000 ppm on pollen germination and tube growth was studied in four species: <em>Antirrhinum majus, Campanula rapunculoides, Eschscholtzia californica</em> and <em>Narcissus poeticus</em>. Pollen grains showed different sensitivity to particular concentrations of morphactin. Although the concentration of 1000 ppm inhibited completely pollen germination in <em>A. majus</em> and <em>C. rapunculoides</em>, it was not sufficient to suppress this process in <em>E. californica</em> and <em>N. poeticus</em>. Morphactin has a different effect on the percentage of germinated and ruptured pollen, tube length and its rate of growth. This effect was either inhibitory, neutral or stimulatory, depending on the morphactin concentration, the time of culture and species studied. The action of morphactin on the germination process is discussed.

scholarly journals Effects of fluorides and sulphur dioxide on pollen germination and growth of the pollen tube

2014 ◽  
Vol 54 (2) ◽  
pp. 125-129 ◽  
Author(s):  
Leon Mejnartowicz ◽  
Andrzej Lewandowski
Keyword(s):  
Air Pollution ◽  
Pollen Tube ◽  
Sulphur Dioxide ◽  
Pollen Germination ◽  
Pollen Grains ◽  
Phosphate Fertilizer ◽  
Tube Length ◽  
Pine Pollen ◽  
Germination And Growth ◽  
Pollen Tube Length

The action of fluorides and sulphur dioxides from emissions from a phosphate fertilizer factory significantly reduced the germination of Scots pine pollen grains. The pollen tube length indicated that its growth is uninhibited even though the pollen was collected under conditions of air-pollution. There are statistically significant differences showing longer tubes in the sensitive trees pollen grains. l he ageing of pollen from the sensitive trees occurs probably more rapidly than in the tolerant trees.

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