scholarly journals Effects of storage temperature and duration on pollen grain viability and pollen-tube elongation in Chinese chinqapin (Castanea henryi Skan)

2020 ◽  
Vol 49 (2) ◽  
pp. 297-304
Author(s):  
Huan Xiong ◽  
Deyi Yuan ◽  
Zhi-Yu Deng ◽  
Genhua Niu ◽  
Feng Zou
Keyword(s):  
Pollen Tube ◽  
Room Temperature ◽  
Storage Temperature ◽  
Southern China ◽  
Germination Rate ◽  
Pollen Viability ◽  
Pollen Grain ◽  
Tube Length ◽  
Storage Duration ◽  
Pollen Tube Length

Chinese chinquapin [Castanea henryi (Skan) Rehder & E.H. Wilson] is used as a food and timber crop in southern China. Most chinquapin cultivars are self-incompatible and bloom at different times; consequently, artificial pollination is used to ensure fruit set and nut yield. Effective pollen storage that enables producers and breeders to use stored pollen for cross-pollination at a later date is important. In this study, the cultivar Changmangzi was used to estimate the viability and pollen tube length of pollen stored at room temperature, and at 4, −20, and −80°C using in vitro germination tests. It was observed that pollen grain germination significantly decreased at all four storage temperatures. Pollen viability was 14.4% after only 24 days of storage at room temperature. The germination rate was 13.3% after 90 days of storage at 4°C, and 14.5% after 180 days at −20°C. The initial germination rate of pollen stored at −80°C was 56.3% at the beginning of the test and decreased to 15.4% after 240 days. Pollen-tube length decreased with increased storage duration; mean pollen-tube lengths ranged from 109.44 to 257.51 μm. Based on these results, it is suggested that a storage temperature of −80°C for Changmangzi pollen is good.

scholarly journals Pollen viability in Quercus robur L.

2017 ◽  
Vol 69 (1) ◽  
pp. 111-117 ◽  
Author(s):  
Branislava Batos ◽  
Danijela Miljkovic
Keyword(s):  
Pollen Tube ◽  
Quercus Robur ◽  
Interindividual Variability ◽  
Germination Rate ◽  
Pollen Viability ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Tube Length ◽  
Pedunculate Oak ◽  
Pollen Tube Length

The variability of viability (germination rate and the length of pollen tubes) of fresh pedunculate oak (Quercus robur L.) pollen grains was studied in vitro on a medium containing 15% sucrose. Spatial variability was studied by sampling fresh pollen grains from a total of thirteen trees at four different sites in the area of Belgrade (Kosutnjak, Banovo Brdo, Ada Ciganlija and Bojcin Forest) in a single year (2011). In order to assess temporal variability and determine the effects of climate change on a small time scale, we studied the viability of the pollen grains collected from one tree at the Banovo Brdo site in six different years (2004, 2005, 2006, 2007, 2011 and 2012). Interindividual variability was tested on the pollen grains sampled from eight trees at Ada Ciganlija in 2004. The percentage values of the pollen grain germination rate and the pollen tube length showed no statistically significant differences between the sites. However, the studied characteristics of the pollen grain viability (germination rate and pollen tube length) showed statistically significant differences in both temporal (between the pollen collection years) and interindividual variability. This type of research makes a valuable contribution to pedunculate oak breeding programs through the identification of trees with stable production and a good quality of pollen. Furthermore, it can be important in defining the patterns of spatial, temporal and individual variability of pollen grain viability under the influence of climate factors, which are showing compelling changing trends from year to year.

scholarly journals Assessment of Pollen Viability, Germination, and Tube Growth in Eight Tunisian Caprifig (Ficus carica L.) Cultivars

ISRN Agronomy ◽  
2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Badii Gaaliche ◽  
Afifa Majdoub ◽  
Mehdi Trad ◽  
Messaoud Mars
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Germination Rate ◽  
Pollen Viability ◽  
Tube Growth ◽  
Tube Length ◽  
Tetrazolium Chloride ◽  
North West ◽  
Ttc Test

The evaluation of pollen viability and its germination capacity are two essential criteria for pollinator’s characterization. This study was carried out to evaluate pollen quality of eight caprifigs grown in the center-east and north-west of Tunisia. Two colorimetric tests 2,3,5-triphenyl tetrazolium chloride (TTC) and acetocarmine were used to estimate pollen viability. Germination rate and pollen tube growth in a culture medium containing 5% sucrose, 5 ppm boric acid (H3BO3) and 1% agar were registered after 24, 48, and 72 hours (H) of incubation. Results showed that the highest pollen viability rate (84%) was obtained by TTC test in caprifig Assafri, followed by Jrani (80.2%), Djebba 2 (77.8%), and Djebba 1 (73.6%). That of other caprifigs did not exceed 50% for the two tests. In all caprifig types, germination rate and pollen tube growth varied according to the incubation period. The highest percentage of germination (72%) and maximum pollen tube length (960 μm) were recorded after 72 H of incubation in caprifigs Assafri and Jrani, respectively. Among the caprifigs studied, four types (Assafri, Jrani, Djebba 1, and Djebba 2) appeared to be suitable pollinators with respect to the criteria investigated.

scholarly journals Longevity and germination of Juniperus communis L. pollen after storage

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Andrej Kormuťák ◽  
Peter Bolecek ◽  
Martin Galgóci ◽  
Dušan Gömöry
Keyword(s):  
Pollen Tube ◽  
Pollen Viability ◽  
Germination Percentage ◽  
Tube Length ◽  
In Vitro Germination ◽  
Short Term ◽  
Term Storage ◽  
Pollen Tube Length ◽  
Common Juniper

AbstractPollen storage belongs among the most important activities associated with pollen handling. It overcomes the differences in pollen shedding and ovule receptivity during controlled pollination experiments. It is especially important for species like common juniper (Juniperus communis L.) with an extremely low quality of seeds due to pollination failure. Additionally, it is a substantial part of germplasm preservation programmes in pollen banks. In the present paper, the effect of short-term storage of pollen was studied using pollen samples from five shrubs in an in vitro germination test. Two temperature regimes were tested. The pollen viability of freshly collected pollen varied considerably between individual shrubs, exhibiting 67.3–88.6% germination rate and 248.0–367.3 µm of pollen tubes. Storage at + 4 °C for four months was accompanied by a profound decline in pollen viability. The germination percentage was reduced to 49.2–75.2% and the pollen tube length to 32.5–69.0%, depending on individual shrubs. The corresponding decline in pollen viability characteristics during storage at − 20 °C was only negligible in two of the tested shrubs. In the remaining three shrub samples, an increase in germination percentage was observed. Pollen tube growth responded more sensitively to freezing, but, on average, the decrease in length was lower than that at + 4 °C. The rate of reduction in pollen tube length varied between 11.5 and 45.4%. Cytological events accompanying in vitro germination of freezer-stored pollen exhibited some delay in releasing the exine from pollen grains during the early stages of germination as compared with freshly collected pollen. In conclusion, short-term storage of the common juniper pollen in a freezer is better for the preservation of its viability than storage at + 4 °C.

scholarly journals Factors to affect inbred beet plants while developing material for linear selection

2019 ◽  
Vol 23 (4) ◽  
pp. 439-447 ◽  
Author(s):  
M. I. Fedorova ◽  
E. G. Kozar ◽  
S. A. Vetrova ◽  
V. A. Zayachkovskyi ◽  
V. A. Stepanov
Keyword(s):  
Pollen Tube ◽  
Recurrent Selection ◽  
Germination Rate ◽  
Pollen Viability ◽  
Tube Length ◽  
Self Incompatibility ◽  
Seed Productivity ◽  
The Third ◽  
Male Gametes

Considering its capacities, the generative system of Beta vulgaris L. is regarded as highly productive. While inbreeding, the reproductive potential of cross-pollinated beet plants with gametophytic self-incompatibility (SI) changes significantly and is determined by a joint effect of multiple factors including the level of inbred depression. In the present study, original data have been obtained revealing relationships between inbred beet seed productivity, its self-incompatibility and microgametophyte parameters, which is crucial for developing and maintaining constant fertile beet lines. It has been discovered that inbred depression increases the number of sterile microgametes and anomalous pollen grains, reduces pollen fertility and the length of pollen tubes. As a result, the seed yield in inbred beet progeny, including SI ones, reduces significantly just after the third inbreeding. At the same time, highly productive inbred beet is characterized by a lower rate of pollen tube growth in vitro. In inbred plants, there is no close relationship between pollen viability and seed productivity, because the elimination of germinated male gametes and degeneration of seed embryos may go over the entire period of fertilization starting its progamic phase. The SI plants have more degenerating embryos than self-fertile ones, but seed vessel outgrowth in the seeds with abortive embryos makes them morphologically similar to fertile seeds. For that reason, when assessing inbred beet plants based on their self-incompatibility/self-fertility, one should consider the qualitative characteristics of the seeds. Using the method of recurrent selection based on such factors as seed productivity, pollen tube length and field germination rate increase the output of plant forms with a potentially high self-compatibility in their progeny. To support such genotypes in the progeny, one has to, starting from the third inbreeding, perform sib crossing to reduce the negative effect of inbred depression and self-incompatibility.

scholarly journals Palynological investigations in Jasminum spp.

2020 ◽  
Vol 15 (2) ◽  
pp. 183-190
Author(s):  
M Ganga ◽  
J Lakshmi ◽  
N Manivannan ◽  
K Rajamani
Keyword(s):  
Pollen Germination ◽  
Tamil Nadu ◽  
Landscape Architecture ◽  
Germination Rate ◽  
Pollen Grain ◽  
Tube Length ◽  
Exine Ornamentation ◽  
Breeding Programmes ◽  
Pollen Tube Length

The present investigation was carried out at the Department of Floriculture and Landscape Architecture, Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore during 2017-2019. The study involved nine jasmine genotypes, four falling under the commercially cultivated types and five belonging to underutilized species or ‘lesser-known species’. The study was undertaken to investigate and document the palynological parameters of jasmines which could serve as a reliable reference for future jasmine breeding programmes. The palynological investigations were carried out using Scanning Electron Microscopy (SEM), haemocytometry, acetocarmine test, and in vitro pollen germination. The pollen morphology analysis indicated wide variation among the species for the shape of the pollen grain, ranging from tricolpate to prolate; the exine ornamentation was reticulate in all the genotypes. Pollen output was the highest in J. rigidum (28,660 pollen/flower) and the lowest in (625 pollen/ flower) in J. sambac cv. Ramanathapuram Gundumalli. The maximum pollen germination rate and pollen tube length were recorded in J. rigidum.

scholarly journals GENETIC ANALYSIS OF THE TOMATO POLLEN BEHAVIOUR IN VITRO UNDER LOW TEMPERATURES

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 584c-584
Author(s):  
P. J. Kaltsikes ◽  
P. J. Bebeli
Keyword(s):  
Pollen Tube ◽  
Pollen Germination ◽  
Genetic Model ◽  
Germination Rate ◽  
Tube Length ◽  
Gene Effects ◽  
Length Determination ◽  
Microscope Slides ◽  
Pollen Tube Length

The genetics of the ability of tomato pollen to germinate and grow in vitro under low temperature was investigated in two crosses namely “Resista” × “Hilda” and “Resista” × “Monita”. In each cross the following generations were utilised: F1, F2, BC1 and BC2, and their reciprocals, along with the parents. Pollen was placed on microscope slides having cavities filled with a liquid nutrient medium (water, 10% sucrose and 50 ppm boric acid) and allowed to germinate and grow for six hours at 15° C and then killed with acetocarmine. Germination rates and pollen tube length were determined and analyses on a genetic model allowing only for additive and dominance gene effects. For pollen germination rate both additive and dominance gene effects were significant while for tube length only the additive effects were. Dominance was towards lower rates of germination. At least three genes control pollen germination rates while seven or more are involved in pollen tube length determination.

scholarly journals Pollen morphology and viability of some Indian mangroves

2008 ◽  
pp. 60-71 ◽  
Author(s):  
Suparna Gupta ◽  
Arunima Ghosh ◽  
Subrata Maity ◽  
Sauren Das
Keyword(s):  
Pollen Tube ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Tube Length ◽  
Strong Positive Correlation ◽  
Viable Pollen ◽  
The Family ◽  
Indian Mangroves ◽  
Pollen Tube Length

A study on pollen morphology of four common Indian mangroves from Sundarbans mangrove swamps, three species from the family Rhizophoraceae (Bruguiera sexangula Laur., Ceriops tagal Perr.and Rhizophora mucronata Lamk.) and one species from the family Avicenniaceae (Avicennia marina Forsk.) was carried out. Shape of the pollen grains were prolate, prolate spheroidal and subprolate with tricolporate apertures. Surface ornamentation of the studied taxa was reticulate, finely reticulate or scabrate. Pollen grain viability of the said taxa was determined by stain test (1% aceto-orcein) under in situ condition and percentage of viable pollen production was recorded. The result revealed that all the studied taxa produce a fairly good amount of viable pollen grains in natural condition, an indication that they are good propagule producer in the long run. Pollen grain germination and measurements of pollen tube length using different sucrose concentrations (0.2, 0.6, 1.0, 1.2, 1.6 and 2.0%). Maximum pollen fertility and pollen tube length occurred in 2% sucrose solution in case of A. marina and B. sexangula, whereas 1.6% in C. tagal and 1.0% in R. mucronata. A strong positive correlation exist between pollen germination and pollen tube length, but there was a hardly relation between sucrose concentration and pollen tube length.

scholarly journals Overcoming Pre-Fertilization Barriers in Intertribal Crosses between Anemone coronaria L. and Ranunculus asiaticus L.

Horticulturae ◽  
2021 ◽  
Vol 7 (12) ◽  
pp. 529
Author(s):  
Emmy Dhooghe ◽  
Dirk Reheul ◽  
Marie-Christine Van Labeke
Keyword(s):  
Pollen Tube ◽  
Seed Set ◽  
Sperm Nucleus ◽  
Tube Length ◽  
Egg Cell ◽  
Dichlorophenoxyacetic Acid ◽  
Style Length ◽  
Ranunculus Asiaticus ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Pollen Tube Length

Hybridization in flowering plants depends, in the first place, on the delivery of pollen to a receptive stigma and the subsequent growth of pollen tubes through the style to the ovary, where the sperm nucleus of the pollen grain can ultimately fertilize the egg cell. However, reproductive failure is often observed in distant crosses and is caused by pre- and/or post-zygotic barriers. In this study, the reproductive pre-fertilization barriers of intertribal crosses between Anemone coronaria L. and Ranunculus asiaticus L., both belonging to the Ranunculaceae, were investigated. Despite the incongruity of intertribal crosses between A. coronaria and R. asiaticus having been of low intensity at the stigmatic level, interstylar obstructions of the pollen tube growth occurred, which confirmed the presence of pre-fertilization barriers. We show that these barriers could be partially bypassed by combining pollination with a stigma treatment. More specifically, a significantly higher ratio of the pollen tube length to the total style length and a better seed set were observed when the stigma was treated with the auxin 2,4-dichlorophenoxyacetic acid (2,4-D, 1 mg.mL−1) together with the cytokinin kinetin (KIN, 0.5 mg.mL−1) 24 h after pollination, irrespective of the cross direction. More specifically, the stigma treatments with any form of auxin (combined or not combined with cytokinin) resulted in a full seed set, assuming an apomictic fruit set, because no pollination was needed to obtain these seeds.

Differences in Style Length and In vitro Germinated Pollen Tube Length and Other Reproductive Structures of Tetraploid and Diploid Cottons

2000 ◽  
Vol 3 (9) ◽  
pp. 1372-1374
Author(s):  
Mohammed Jurial Baloch . ◽  
Abdul Rahim Lakho . ◽  
Hidayatullah Bhutto . ◽  
Mohammad Hussain Arain .
Keyword(s):  
Pollen Tube ◽  
Tube Length ◽  
Style Length ◽  
Reproductive Structures ◽  
Germinated Pollen ◽  
Pollen Tube Length

Unilateral Cross-Incompatibility in Eucalyptus: the Case of Hybridisation Between E. globulus and E. nitens

1990 ◽  
Vol 38 (4) ◽  
pp. 383 ◽  
Author(s):  
PL Gore ◽  
BM Potts ◽  
PW Volker ◽  
J Megalos
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Tubes ◽  
Full Length ◽  
Tube Length ◽  
Style Length ◽  
Similar Difference ◽  
Structural Barrier ◽  
Pollen Tube Length

The growth of E. globulus and E. nitens pollen tubes in styles of E. globulus was examined in order to elucidate the site of the unilateral barrier to hybridisation. Pollen tubes of E. nitens failed to grow the full length of the larger E. globulus style. E. globulus pollen tubes grew an average of 1.4 mm per day for the first 4 days, compared with 0.8 mm per day for pollen tubes of E. nitens. From days 4 to 14, the growth of E. nitens pollen tubes slowed to an average of 0.2 mm per day and virtually no growth occurred after day 14. In contrast, E. globulus pollen tubes grew through the style and into the ovary between days 5 and 14. By day 28, at about the time of style abscission, E. nitens tubes had grown only 6 mm, well short of the full length of the E. globulus style (9-10 mm). A similar difference in growth was obtained in vitro where E. nitens pollen tubes were significantly shorter than those of E. globulus. A comparison also including E. ovata, E. urnigera and E. gunnii indicated a significant correlation between style length and in vitro pollen tube length. It is argued that the unilateral cross-incompatibility between E. globulus and E. nitens is due to a structural barrier arising from an inherent limit to pollen tube growth which is associated with pistil size.

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