scholarly journals Correlation Analysis of Antioxidant Activities with Tannin, Total Flavonoid, and Total Phenolic Contents of Nutmeg (Myristica fragrans Houtt) Fruit Precipitated by Egg white

2021 ◽  
Vol 4 (4) ◽  
pp. 301-310
Author(s):  
Irma Antasionasti ◽  
Olvie Syenni Datu ◽  
Utami Sasmita Lestari ◽  
Surya Sumantri Abdullah ◽  
Imam Jayanto
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Egg White ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Stable Free Radical ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Myristica Fragrans

The nutmeg (Myristica fragrans) flesh extract has a strong antioxidant activity. Therefore, M. fragrans flesh can be developed for functional drinks which are sources rich in antioxidants good for the prevention and treatment of diseases such as cancer and cardiovascular diseases. However, the tannins' content can cause a bitter and sour taste. Therefore, the tannins content should be reduced by the addition of egg white. The purpose of this study is to find out the comparison of antioxidant activity between a combination of M. fragrans flesh extract with various concentrations of egg white and to correlate its antioxidant activities with tannin, total flavonoid, and total phenolic contents. The antioxidant activities were conducted on M. fragrans flesh extract by using DPPH and ABTS radicals. Tannin, total flavonoid, and total phenolic contents from M. fragrans flesh extract were also tested. The M. fragrans flesh extracts without addition egg white have a strong antioxidant in scavenging the stable free radical ABTS (89.980±0.480 µg/mL) and intermediate antioxidant in scavenging the stable free radical DPPH (105.669±0.102 µg/mL). It is followed accordingly by tannin, total flavonoid, and total phenolic contents, namely 14.034±0.100 %w/w TAE, 26.929±0.129 %w/w QE, and 53.164±0.129 %w/w GAE, respectively. Correlation of tannin, total flavonoid, and total phenolic contents, which inhibited DPPH and ABTS radicals had R2 values of about 89.23-97.63%. It showed that antioxidant activity is strongly influenced by the tannin, total flavonoid, and total phenolic contents. Therefore, precipitation from the tannin-protein bond caused antioxidant activities were decreased.

SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN DAN ANTITUMOR 20 TUMBUHAN OBAT ASAL KABUPATEN MANOKWARI

2008 ◽  
Vol 7 (1) ◽  
Author(s):  
Bimo Budi Santoso
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Antitumor Activity ◽  
Traditional Method ◽  
Extraction Efficiency ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Total Phenolic Contents

<strong><em>Flavanoid, steroid, and terpenoid contents of 20 Manokwari medicinal plants were analyzed.  The antioxidant activity, total phenolic contents and antitumor activity of 20 Manokwari medicinal plants were also evaluated. The result shows that 83% positif to flavanoid test, 59% positif to terpenoid test and only 25% positif steroid. Antioxidant activity and total phenolic contents evaluated using Ferric Thiocianate (FTC) and Folin-Ciocalteu methods respectively. Antioxidant activity and total phenolic contents of medicinal plants were extracted by the traditional method, boiling in water and also in 80% methanol. Twenty plants evaluated in both exstracts have significantly varies of antioxidant activities and phenolic contents,  A significant and linier correlation coefficient between the antioxidant activity and the total phenolic content was found in both aqueous (R<sup>2</sup>= 0,77) and methanol (R<sup>2</sup> = 0,85). Antitumor activity was tested using cell maurine P-388 and only 2 of medicinal plants are active to inhibit cell maurine P-388. Comparing extraction efficiency of the two methods, the methanol extracted phenolic compounds more efficiently, and antioxidant activity of the extract was higher.</em></strong>

scholarly journals Comparison of Total Phenolic Contents and Antioxidant Activities of Centella asiatica Extracts Obtained by Three Extraction Techniques

2019 ◽  
Vol 6 (2) ◽  
pp. 42-49
Author(s):  
Wan Nurul Huda Wan Zainal ◽  
FARA RESTINA MUSAHIB ◽  
NABILAH SYAFIQAH ZULKEFLEE
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Centella Asiatica ◽  
Extraction Yield ◽  
Total Phenolic ◽  
Extraction Techniques ◽  
Phenolic Contents ◽  
Total Phenolic Contents

Through different extraction techniques, the potential of Centella asiatica as a natural source of antioxidant was investigated. The C. asiatica aqueous extracts were obtained via infusion, decoction and ultrasonic-assisted extraction (UAE) techniques. The effects of different extraction techniques were studied on the extraction yield, total phenolic contents and antioxidant activity. The total phenolic contents of the extracts and antioxidant activity were examined using the Folin-Ciocalteu’s reagent and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity assay, respectively. Results indicated that the UAE exhibited the highest extraction yield, highest total phenolic contents, as well as highest antioxidant activity. The yield of the extracts increased in the order of infusion < decoction < UAE which were 18.2 %, 23.6% and 25.4 %, respectively. All extraction techniques had a significant effect (p <0.05) on the total phenolic contents and antioxidant activity of C. asiatica extracts. The total phenolic contents was ranged from 3.42 ± 0.030 to 8.32 ± 0.105 mg GAE/g dry extract while the antioxidant activity was in the range of 75 to 86 %. This study confirms that C. asiatica has the potential to be a good resource for the future development of natural antioxidant. In addition, extraction via UAE can be an ideal technique to obtain phytochemical-rich extracts from medicinal plants.

scholarly journals Antioxidant, Antimicrobial Activity and Toxicity Test ofPilea microphylla

2010 ◽  
Vol 2010 ◽  
pp. 1-6 ◽  
Author(s):  
Amir Modarresi Chahardehi ◽  
Darah Ibrahim ◽  
Shaida Fariza Sulaiman
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Toxicity Test ◽  
Radical Scavenging ◽  
Chloroform Extract ◽  
Total Flavonoid ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Total Phenolic Contents

A total of 9 plant extracts were tested, using two different kinds of extracting methods to evaluate the antioxidant and antimicrobial activities fromPilea microphylla(Urticaceae family) and including toxicity test. Antioxidant activity were tested by using DPPH free radical scavenging, also total phenolic contents and total flavonoid contents were determined. Toxicity assay carried out by using brine shrimps. Methanol extract of method I (ME I) showed the highest antioxidant activity at69.51±1.03. Chloroform extract of method I (CE I) showed the highest total phenolic contents at72.10±0.71and chloroform extract of method II (CE II) showed the highest total flavonoid contents at60.14±0.33. The antimicrobial activity ofPilea microphyllaextract was tested in vitro by using disc diffusion method and minimum inhibitory concentration (MIC). ThePilea microphyllaextract showed antibacterial activity against some Gram negative and positive bacteria. The extracts did not exhibit antifungal and antiyeast activity. The hexane extract of method I (HE I) was not toxic against brine shrimp (LC50 value was 3880 μg/ml). Therefore, the extracts could be suitable as antimicrobial and antioxidative agents in food industry.

scholarly journals Total Phenolic Contents, Antioxidant Activities and Organic Acids Composition of Three Selected Fruit Extracts at Different Maturity Stages

2014 ◽  
Vol 2 (2) ◽  
pp. 40-46
Author(s):  
Seri Intan Mokhtar ◽  
Chee Leong Pheen ◽  
Ee Ven Lee ◽  
Nur Ain Abd Aziz
Keyword(s):  
Antioxidant Activity ◽  
Citric Acid ◽  
Organic Acids ◽  
Organic Acid ◽  
Malic Acid ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Young Stage

The total phenolic contents (TPC), antioxidant activities and organic acid composition of Salacca zalacca, Mangifera indica L. Chok Anan and Baccaurea motleyana Hook. F. extracts at different maturity stages were evaluated by using modified Folin-Ciocalteu assay at 765 nm absorbance, 2,2- diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and HPLC RP18 column at 215 nm. TPC content ranged from 63.9 mg GAE/100g to 381.23 mg GAE/100g, whereas the antioxidant activity ranged from 3.10% - 84.45%. The M. indica L. Chok Anan contained malic acid and citric acid in the range of 43.897-73.396 mg/100g FW and 2.681-18.428 mg/100g FW at young to ripe stages, respectively. B. motleyana Hook. F. contained citric acid and tartaric acid in the range of 4.649-6.114 mg/100g FW and 3.645-4.365 mg/100g FW at young to ripe stages, respectively. S. zalacca contained malic acid and citric acid in the range of 4.270-17.926mg/100g FW and 0.845-3.284 mg/100g FW at young to ripe stages, respectively. The highest TPC and antioxidant activity were found in the young stage of S. zalacca fruit extract with lowest inhibitory concentration (IC50) valued at 0.57 mgml-1. There was a positive linear correlation between the TPC and antioxidant activities of the fruit extracts. Malic acid is the predominant organic acid in M. indica L. Chok Anan and S. zalacca, while citric acid is the predominant organic acid in B. motleyana Hook. F. This research suggests that the studied fruits at young stage are a better source of TPC and antioxidants. The tested fruits showed a decrease in the concentration of organic acids with ripening.

scholarly journals Determination of Total Phenolic Contents and Antioxidant Activity of the Roots of Pimpinella candolleana Wight & Arnott

2019 ◽  
Vol 31 (2) ◽  
pp. 120-124
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Colorimetric Method ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Scavenging Effect ◽  
Total Phenolic Contents ◽  
Methanolic Extracts

Pimpinella candolleana Wight & Arnott belonging to family Apiaceae is a valuable medicinal plant. The plant specimens were collected from Pindaya Township, Southern Shan State in July 2014. The total phenolic contents in the aqueous, ethanolic and methanolic extracts of the roots of Pimpinella candolleana Wight & Arnott were determined at Food and Drug Administration Department, Mandalay by Folin-Ciocalteu colorimetric method using gallic acid as the standard. The antioxidant activity of the different concentrations (100 µg/ml, 200 µg/ml, 300 µg/ml, 400 µg/ml and 500 µg/ml) of three extracts was evaluated at Department of Medical Research (Pyin Oo Lwin Branch) by DPPH free radical scavenging method. The total phenolic contents of aqueous, ethanolic and methanolic extracts of roots were 83.63 mg GAE/g extract, 93.87 mg GAE/g extract and 118.85 mg GAE/g extract, respectively. The IC50 values of aqueous, ethanolic and methanolic extracts were 69.183 µg/ml, 63.096 µg/ml and 31.622 µg/ml, respectively. The results showed that there is a positive correlation between free radical scavenging effect and total phenolic contents. Thus, this study scientifically proved that this resource sample is rich in total phenolic contents and significantly has antioxidant activity.

scholarly journals Evaluation of Total Phenolic, Total Flavonoid Content And Antioxidant Activity of Rhus Vulgaris

2021 ◽  
Author(s):  
Aderaw Anteneh Belew ◽  
Getachew G/Mariam W/Hana ◽  
Desta Shumuye Meshesha ◽  
Mulugeta Legese Akele
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Gallic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Flavonoid ◽  
Free Radical Scavengers ◽  
Total Phenolic ◽  
Phenolic Contents

Abstract Background: Rhus vulgaris is rich in various classes of polyphenols and flavonoids that act as free radical scavengers and reduce oxidative stress and cure various harmful human diseases. The plant is a traditionally known medicinal plant which is used against a number of diseases including cancer. Methods: The current investigation points towards the investigating quantitative phenolic contents, flavonoid contents and the free radical scavenging activity & antioxidant activity of Rhus vulgaris extract in n-hexane, acetone, and 80% of aqueous methanol. The phenolic contents were estimated by Folin–Ciocalteu procedure and gallic acid as a reference molecule whereas the total flavonoid contents were determined by using aluminum chloride and catechin. In addition, the dilution serial method was used to evaluate the leaves extract of rhus vulgaris, and the DPPH (2,2-diphenyl-1-picrylhydrazyl) method was utilized to assess the above-mentioned extracts against oxidative stress. Results: The result revealed that the ranges of total phenolic content from 5.82 ± 4.6 to 83.15 ± 7.6 mg GAE/g of the dry weight of extract, expressed as gallic acid equivalents. The total flavonoid concentrations were varied from 2.21 ± 7.34to 23.47 ± 4.87 mg CE/g, expressed as catechin equivalents. Antioxidant activity of extracts was expressed as the concentration of DPPH radical’s inhibition ranges from 1.2 ± 0.32 to 22.86 ± 3.71 mg AAE/ g. Conclusion: The 80% aqueous methanolic extract of Rhus vulgaris showed the highest phenolic and flavonoid contents and strong antioxidant potential and it could be used as antibiotics for different curable and incurable diseases.

scholarly journals Effects of Various Drying Methods on Selected Physical and Antioxidant Properties of Extracts from Moringa oliefera Leaf Waste

2020 ◽  
Vol 12 (20) ◽  
pp. 8586
Author(s):  
Ade Chandra Iwansyah ◽  
Tran Dinh Manh ◽  
Yusuf Andriana ◽  
Muhammad Aiman bin Hessan ◽  
Faridah Kormin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Physical Properties ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Drying Methods ◽  
Sun Drying ◽  
Phenolic Contents ◽  
Tray Drying

This study aims to evaluate the effects of different drying methods on the physical properties, total phenolic content, total flavonoid content, and antioxidant activity of extracts from Moringa oliefera L. (MO) leaf waste. The effects of two drying techniques, namely, sun drying (A1) and tray drying (A2), on the physical and antioxidant properties of the extracts obtained using three extracting solvents, i.e., water (b1), ethanol (b2), and ethyl acetate (b3), were investigated. These extracts were analyzed for their physicochemical and antioxidant properties. The antioxidant properties were determined with the Folin Ciocalteau, aluminum chloride, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays. Fourier transform infrared spectrophotometry (FTIR) was used to identify functional groups in the active compounds. It was found that the physical properties of the MO extract, including yield, pH, total solids, and color, showed significant differences for the two drying methods (p < 0.05). The b3 extract had the highest value for total phenolic contents, total flavonoid contents, and antioxidant activities, followed by b2 and b1, respectively, for both the sun drying and tray drying methods. There was a significant correlation between the total phenolic and flavonoid contents and antioxidant activity (IC50). This study reveals that waste material from MO leaves could be utilized as an antioxidant agent, which is expected to reduce environmental pollution from the MO processing industry.

scholarly journals IN VITRO ANTIOXIDANT ACTIVITY, TOTAL PHENOLIC CONTENTS AND PHYTOCHEMICAL EVALUATION FROM CRUDE EXTRACTS OF AN ENDANGERED PLANT CORDIA MACLEODII HOOK. F. THOMSON

2019 ◽  
Vol 8 (3) ◽  
Author(s):  
Jagriti Chandrakar ◽  
Satish Dubey ◽  
Kundan Ojha ◽  
Rashmi Dehariya ◽  
Ashwini Kumar Dixit
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Hepatoprotective Activity ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Successive Extraction

Cordia macleodii, an important medicinal plant traditionally used for the treatment of various disorders, including wound healing, jaundice, gastric ulcer, hepatoprotective activity and antivenom potential, thus the plant showed the high antioxidant properties. Successive extraction of leaf and barks powder with methanol and water was tested for quantitative determination of total phenolics, total flavonoids and various in vitro antioxidant activities. Phytochemical screening of crude plant extracts revealed the presence of sterols, alkaloid, flavonoid, phenolic, Reducing sugar, glycosides, tannins and saponins. All extracts showed the significant total phenolic and flavonoid contents and as well as radical scavenging and iron-chelating activities. Among all, methanol leaf extract was observed to be higher antioxidant activity than that of other extract. The methanol extracts showed greater antioxidant activity by DPPH scavenging the free radical with IC50 values of 7.63 ± 0.38 μg gm-1 along with scavenged free radical in a concentration depended manner, which is compare to the standard ascorbic acid.  Our results revealed that the presence of grater antioxidant activity of C. macleodii extract may be due to the high level of flavonoid and phenolic content in the plant. These results clearly indicated that C. macleodii is an effective natural antioxidant. Key words: Cordia macleodii, antioxidant, radical scavenging, phenolics, flavonoid, phytochemical.

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