scholarly journals Uji Aktivitas Bakteri Salmonella Thypii pada Sayuran Lalapan Kemangi (Ocimum Sanctum L) Secara In Vitro

2021 ◽  
Vol 6 (2) ◽  
pp. 129-136
Author(s):  
Andi Muhammad Munawir ◽  
Anastasia Basir
Keyword(s):  
Ocimum Sanctum ◽  
Foodborne Disease ◽  
Probability Sampling

Keamanan pangan menjadi salah satu masalah kesehatan yang perlu diperhatikan karena konsumsi pangan yang tercemar oleh mikroorganisme atau bahan kimia yang dapat menyebabkan suatu penyakit yang disebut foodborne disease. Daun kemangi mampu menghambat pertumbuhan bakteri salah satunya Salmonella typhii. Penelitian ini bertujuan untuk mengetahui ada atau tidaknya bakteri Salmonella typhii pada sayuran lalapan kemangi (Ocimum sanctum L) menggunakan pengujian Biokimia. Penelitian ini dilaksanakan di Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Hasanuddin Makassar. Metode yang digunakan dalam penelitian adalah rancangan eksperimental dengan teknik probability sampling berjumlah 10 sampel pada 10 lokasi berbeda di pasar Kota Makassar, dilakukan dengan identifikasi bakteri menggunakan medium selektif, kemudian isolasi bakteri dengan medium SSA dan medium NA, kemudian dilakukan uji Biokimia kemudian dilakukan pewarnaan gram. Sampel yang teridentifikasi bakteri Salmonella typhii dilakukan uji daya hambat dengan medium MHA dan antibiotik, selanjutnya dilakukan pengukuran zona hambat yang terbentuk dengan menggunakan jangka sorong. Hasil penelitian menunjukkan bahwa dari 10 sampel dengan kode S1-S10, didapatkan pada sampel S1 terdapat bakteri Salmonella typhii. Kesimpulan bahwa terdapat bakteri Salmonella typhii pada sayuran lalapan kemangi yang diambil di pasar Kota Makassar.

scholarly journals Selection and Micropropagation of an Elite Melatonin Rich Tulsi (Ocimum sanctum L.) Germplasm Line

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 207
Author(s):  
Mukund R. Shukla ◽  
Annaliese Kibler ◽  
Christina E. Turi ◽  
Lauren A. E. Erland ◽  
J. Alan Sullivan ◽  
...  
Keyword(s):  
In Vitro Selection ◽  
Integrated Approach ◽  
Antioxidant Potential ◽  
Ocimum Sanctum ◽  
Natural Health Products ◽  
Seed Population ◽  
Health Products ◽  
Potential Applications ◽  
Medicinal Properties

Tulsi (Ocimum sanctum L.) is a sacred plant of medicinal and spiritual significance in many cultures. Medicinal properties of Tulsi are ascribed to its phytochemicals with antioxidant capabilities. The current study was undertaken to screen a large seed population of Tulsi to select germplasm lines with high antioxidant potential and to standardize protocols for micropropagation and biomass production to produce a phytochemically consistent crop. A total of 80 germplasm lines were established under in vitro conditions and screened for their antioxidant potential determined with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) bioassay. The micropropagation of a selected line, named Vrinda, was established using nodal cultures grown on Murashige and Skoog medium containing benzylaminopurine (1.1 µM), gibberellic acid (0.3 µM), and activated charcoal (0.6%). The antioxidant phytohormones melatonin and serotonin were quantified in the field and greenhouse grown tissues of Vrinda and melatonin levels were found to be consistent in both conditions with higher serotonin levels under field conditions. This integrated approach combining the in vitro selection and propagation offers potential applications in the development of safe, effective, and novel natural health products of Tulsi, and many other medicinal plant species.

IN VITRO LIPID PEROXIDATION INHIBITORY AND ANTI-ARTHRITIC ACTIVITIES OF SOME INDIAN MEDICINAL PLANTS

INDIAN DRUGS ◽  
2012 ◽  
Vol 49 (06) ◽  
pp. 31-35
Author(s):  
A. A Rege ◽  
◽  
P. R. Juvekar ◽  
A. R. Juvekar
Keyword(s):  
Lipid Peroxidation ◽  
Liver Mitochondria ◽  
Tinospora Cordifolia ◽  
Ocimum Sanctum ◽  
Rat Liver Mitochondria ◽  
Total Phenolic ◽  
Lipid Peroxidation Inhibitory Activity ◽  
Gallic Acid Equivalent ◽  
Indian Medicinal Plants

Anti-lipid peroxidation effect of aqueous extracts of Ocimum sanctum, Tinospora cordifolia and Withania somnifera was evaluated against Fe2+-ascorbic acid-induced lipid peroxidation using rat liver mitochondria as model system, whereas, anti-arthritic activity was evaluated by proteinase inhibitory assay. O. sanctum showed potent anti-lipid peroxidation and anti-arthritic activities. T. cordifolia exhibited moderate anti-lipid peroxidation activity, but considerable anti-arthritic activity, whereas, W. somnifera revealed least lipid peroxidation inhibitory activity and considerable anti-arthritic activity. Besides, Folin-Ciocalteu reagent in terms of gallic acid equivalent achieved the total phenolic content and the trend was found to be O. sanctum > T. cordifolia > W. somnifera.

scholarly journals Analisa Aktivitas Antibakteri Rebusan Daun Sirih Dengan Rebusan Daun Kemangi Terhadap Pertumbuhan Bakteri Streptococcus mutans

2020 ◽  
Vol 18 (1) ◽  
pp. 36
Author(s):  
ENNY WILLIANTI ◽  
THEODORA THEODORA ◽  
WAHYUNI DYAH PARMASARI
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Streptococcus Mutans ◽  
Observational Research ◽  
Ocimum Sanctum ◽  
Piper Betle ◽  
Oral Flora ◽  
Betel Leaf ◽  
Growth Of Bacteria

<p><strong>ABSTRACT</strong><strong></strong></p><p><strong> </strong></p><p><strong>Background</strong>: Betel leaf contains essential oils consisting of bethelphenol, kavikol, sesquiterpenes, hydroxycavikol, cavibetol, estragol, eugenol and carvacrol. Essential oils are antibacterial due to the presence of phenol compounds and their derivatives that can denature the bacterial cell proteins. Basil leaves contain compounds from essential oils, namely 1,8-cineole, ß-bisabolene, and methyl eugenol. These three ingredients are soluble to ethanol and can cause damage to the cell membranes of the Streptococcus mutans bacteria, which are members of the normal oral flora but can turn into pathogens if the balance of normal flora is disturbed. The aim of this study was to determine the difference in the activity of the antibacterial  of decoction betel leaf (piper betle L. ) with a decoction of basil leaves (ocimum sanctum) against growth of bacteria <em>Streptococcus mutans</em> (in vitro study).</p><p><strong>M</strong><strong>ethod:</strong> this observational research with disk diffusion techniques. This study observed and measured the diameter of the inhibitory zone in MHA formed by decoction of betel leaf (piper betle L) and basil leaf (ocimum sanctum) in units of millimeters (mm). There were 2 groups with 16 replications.</p><p><strong>R</strong><strong>esults</strong>: the results of the description test showed that the antibacterial activity of the betel leaf decoction and the highest decoction of basil leaf was 17 mm and the lowest was 15 mm, but the average antibacterial value of betel leaf decoction (15,81) greater than the average value of antibacterial activity of basil leaf (15.75). This is because there are chemicals contained in betel leaf similar as contained in basil leaf, namely essential oils.</p><p><strong>Conclusion</strong>: there is no difference in the antibacterial activity of decoction  betel leaf with decoction basil leaf against growth of bacteria <em>Streptococcus mutans</em>.</p><p><strong> </strong></p><p><strong>Keywords</strong>: Betel leaf decoction, basil leaf  decoction, Streptococcus <strong>mutans.      </strong></p><p><strong> </strong></p><p><strong> </strong></p><p><strong>Abstrak</strong><strong></strong></p><p><strong> </strong></p><p><strong>Latar Belakang</strong>: Daun sirih mengandung minyak atsiri yang terdiri dari <em>bethelphenol, kavikol, </em>seskuiterpen, hydroxycavikol,cavibetol, estragol, eugenol dan carvacrol. Minyak atsiri bersifat antibakteri karena adanya senyawa phenol dan turunannya yang dapat mendenaturasi protein sel bakteri. Daun kemangi mengandung senyawa dari minyak atsiri yaitu <em>1,8-cineole</em>, <em>ß-bisabolene</em>, <em>metyl eugenol</em>. Ketiga bahan tersebut memiliki sifat larut terhadap etanol dan dapat menyebabkan kerusakan membran sel bakteri <em>streptococcus mutans</em> yang merupakan anggota flora normal rongga mulut tetapi dapat berubah menjadi patogen jika keseimbangan flora normal terganggu.Tujuan penelitian ini untuk mengetahui perbedaan aktivitas antibakteri rebusan daun sirih (<em>piper betle</em> L) dengan rebusan daun kemangi (<em>ocimum sanctum</em>) terhadap pertumbuhan bakteri <em>Streptococcus mutans</em> (penelitian in vitro).</p><p><strong>Metode</strong>: penelitian observasional ini dengan teknik difusi. Penelitian ini dilakukan dengan mengamati dan mengukur diameter zona hambat pada MHA yang dibentuk oleh rebusan daun sirih (<em>piper betle</em> L) dan daun kemangi (<em>ocimum sanctum</em>) dalam satuan milimeter (mm). Terdapat 2 kelompok dengan replikasi sebanyak 16.</p><p><strong>Hasil</strong> : Hasil uji deskripsi menunjukkan bahwa aktivitas antibakteri pada rebusan daun sirih maupun rebusan daun kemangi yang tertinggi sebesar 17 mm dan yang terendah 15 mm. Tetapi pada nilai rata-rata efektifitas antibakteri rebusan daun sirih (15,81) lebih besar daripada nilai rata-rata efektifitas antibakteri rebusan daun kemangi (15,75). Hal ini dikarenakan ada zat kimia yang terkandung dalam daun sirih mirip dengan yang terkandung dalam daun kemangi, yaitu minyak atsiri.</p><p><strong>Kesimpulan</strong> : tidak ada perbedaan aktivitas antibakteri rebusan daun sirih dengan rebusan daun kemangi terhadap pertumbuhan bakteri <em>Streptococcus </em><em>m</em><em>utans</em>.</p><p><strong> </strong></p><p><strong>Kata kunci</strong>:  rebusan daun sirih, rebusan daun kemangi<em>, Streptococcus mutans</em>.</p><p> </p><p>     </p>

EVALUATION OF SOME MEDICINAL PLANTS AS PUTATIVE HIV-PROTEASE INHIBITORS

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (06) ◽  
pp. 24-28
Author(s):  
A.A Rege ◽  
◽  
A. S Chowdhary
Keyword(s):  
Inhibitory Activity ◽  
In Vitro Study ◽  
Hiv Protease ◽  
Tinospora Cordifolia ◽  
Ocimum Sanctum ◽  
Hiv Protease Inhibitors ◽  
Hiv Reverse Transcriptase ◽  
Anti Hiv ◽  
Potent Inhibitory Activity

Aqueous extracts of Ocimum sanctum Linn., Tinospora cordifolia (Willd.) Miers ex Hook. f. & Thoms, Withania somnifera Dunal, Avicennia officinalis Linn. and Rhizophora mucronata Lam. were included for the present in vitro study. Pepsin was used as a substitute for HIV-protease to evaluate inhibitory activity of these extracts, as pepsin has close resemblance with HIV-protease in proteolytic activity. O. sanctum revealed the highest inhibitory activity followed by R. mucronata. In our earlier study, O. sanctum and R. mucronata exerted anti-HIV activity via multiple mechanisms of action; viz., interference with the gp120 / CD4 interaction and inhibition of HIV-reverse transcriptase. In the present study, they also showed potent inhibitory activity against pepsin enzyme (indirectly against HIV-protease) which may be due their flavonoids content.

Anti Tuberculosis Effect of Ocimum sanctum Extracts in in vitro and Macrophage Culture

2006 ◽  
Vol 6 (3) ◽  
pp. 348-351 ◽  
Author(s):  
Taghi Naserpour Fari . ◽  
Amir Hossein Mohaghe . ◽  
Shahram Shahraki Zah . ◽  
Mohammad Naderi . ◽  
Batul Sharifi Moud .
Keyword(s):  
Ocimum Sanctum ◽  
Macrophage Culture
Sign in / Sign up

Export Citation Format

Share Document