scholarly journals The Prevalence of Bacterial Etiologies Causing Otitis Media with Effusion among Omani Children

2021 ◽  
Vol 2 (1) ◽  
pp. 31-34
Author(s):  
Ahmed Al-Sibani
Keyword(s):  
Polymerase Chain Reaction ◽  
Otitis Media ◽  
Otitis Media With Effusion ◽  
Cross Sectional Study ◽  
Chain Reaction ◽  
Cross Sectional ◽  
Detection Rates ◽  
Polymerase Chain ◽  
Ear Effusion ◽  
Children Under 5

Objectives Our main objective was to determine the prevalence of bacterial etiologies of Otitis Media with Effusion (OME) among Omani children as well as to evaluate the antibiotic susceptibility to penicillins. Methods A prospective cross-sectional study was conducted on 28 Omani children under 5 years of age with OME. The Middle Ear Effusion (MEE) was sent for direct culture and polymerase chain reaction (PCR). Results Out of 38 MEE, 8(21.1%) showed growth on direct culture. The majority of bacteria were sensitive to penicillin. 17 (44.7%) samples were positive using PCR with 5 (29.4%) of them had more than one positive pathogen. The detection rates for H. Influenzae, M. catarrhalis and S. pnemoniae were 31.6%, 18.4% and 10.5% respectively. Conclusion The prevalence of bacteria causing otitis media with effusion among Omani children was not different than the rates reported worldwide however the order of the most common ones differs slightly. Keywords: Otitis media with effusion; Bacteria; Polymerase Chain Reaction.

Detection of Genomic Sequences of Respiratory Syncytial Virus in Otitis Media with Effusion in Children

1992 ◽  
Vol 101 (10_suppl) ◽  
pp. 7-10 ◽  
Author(s):  
Yoshitaka Okamoto ◽  
Kazuo Kudo ◽  
Koji Shirotori ◽  
Misao Nakazawa ◽  
Eiko Ito ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Respiratory Syncytial Virus ◽  
Otitis Media ◽  
Otitis Media With Effusion ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Rsv Infection ◽  
Polymerase Chain ◽  
Syncytial Virus ◽  
Viral Sequences

The reverse transcriptase—polymerase chain reaction and the nested polymerase chain reaction were used for detection of respiratory syncytial virus (RSV) sequences in middle ear effusions collected from children with otitis media. Sequences of RSV were detected in 21 of 34 samples tested. These samples were collected during and/or after natural outbreaks of RSV infection in the community. In those patients from whose nasopharynges RSV was isolated, the viral sequences were highly detectable (75%) in the effusions. These observations suggest RSV as an important factor in the pathogenesis of otitis media with effusion.

A cross-sectional study of Leishmania spp. infection in clinically healthy dogs with polymerase chain reaction and serology in Greece

2002 ◽  
Vol 109 (1-2) ◽  
pp. 19-27 ◽  
Author(s):  
Leonidas S Leontides ◽  
Manolis N Saridomichelakis ◽  
Charalambos Billinis ◽  
Vasilios Kontos ◽  
Alexander F Koutinas ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Chain Reaction ◽  
Cross Sectional ◽  
Leishmania Spp ◽  
Healthy Dogs ◽  
Polymerase Chain

scholarly journals Headache associated with COVID-19: Frequency, characteristics and association with anosmia and ageusia

Cephalalgia ◽  
2020 ◽  
Vol 40 (13) ◽  
pp. 1443-1451 ◽  
Author(s):  
Pedro Augusto Sampaio Rocha-Filho ◽  
João Eudes Magalhães
Keyword(s):  
Polymerase Chain Reaction ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Chi Square ◽  
Cross Sectional ◽  
Severe Intensity ◽  
Chi Square Test ◽  
Polymerase Chain

Objectives To assess the frequency and characteristics of headache in patients with COVID-19 and whether there is an association between headache and anosmia and ageusia. Methods This was a cross-sectional study. Consecutive patients admitted to hospital with COVID-19, confirmed by reverse transcription polymerase chain reaction (RT-PCR) technique, were assessed by neurologists. Results Seventy-three patients were included in the study, 63% were male; the median age was 58 years (IQR: 47–66). Forty-seven patients (64.4%) reported headaches, which had most frequently begun on the first day of symptoms, were bilateral (94%), presenting severe intensity (53%) and a migraine phenotype (51%). Twelve patients (16.4%) presented with headache triggered by coughing. Eleven (15%) patients reported a continuous headache. Twenty-eight patients (38.4%) presented with anosmia and 29 (39.7%) with ageusia. Patients who reported hyposmia/anosmia and/or hypogeusia/ageusia experienced headache more frequently than those without these symptoms (OR: 5.39; 95% CI:1.66–17.45; logistic regression). Patients with anosmia and ageusia presented headache associated with phonophobia more often compared to those with headache without these complaints (Chi-square test; p < 0.05). Headache associated with COVID-19 presented a migraine phenotype more frequently in those experiencing previous migraine ( p < 0.05). Conclusion Headaches associated with COVID-19 are frequent, are generally severe, diffuse, present a migraine phenotype and are associated with anosmia and ageusia.

scholarly journals Identification of Dextran and Sludge–Producing Bacteria in Sugar Cane Juices Using Polymerase Chain Reaction

2017 ◽  
Vol 14 (4) ◽  
pp. 1337-1341
Author(s):  
Farzam Latifi ◽  
Sirous Chehrazi ◽  
Hossein Ansari
Keyword(s):  
Polymerase Chain Reaction ◽  
Cross Sectional Study ◽  
Cane Sugar ◽  
Chain Reaction ◽  
Cross Sectional ◽  
Chemical Washing ◽  
Polymerase Chain ◽  
Physical And Chemical ◽  
Main Factor

ABSTRACT: The microbial contamination due to cane sugar transition to the mills is one of the most important factors in increasing sugar lesions in the factory. This study was aimed to isolate dextran-producing bacteria and determine their genus and species. It is a descriptive cross-sectional study which was conducted in year 2014. For this purpose, 200 samples were collected from sugarcane syrups, and then cultured as pourplate and surface. The bacteria were counted and the DNA extracted from the purified bacteria according to the kit protocol. Then, determination of the genus and species of dextran-producing bacteria was performed by polymerase chain reaction (PCR) using specific primers. Data obtained from biochemical, microbial and PCR showed that around 80 strains of leuconostoc have been detected in samples. The results of this study indicate that leuconostoc mesenteroides is the main factor in the production of dextran in sugarcane and beet manufactories. The above mentioned contamination sources can be minimized by reducing the transfer time of burned sugarcane to the factory, as well as the regular physical and chemical washing of the mills.

scholarly journals Assessment of Adenosine Deaminase Level and the Utility of Polymerase Chain Reaction in Diagnosis of Tuberculous Pleural Effusion

2018 ◽  
Vol 11 (1) ◽  
pp. 5-9
Author(s):  
A Nagila ◽  
S Khanal ◽  
N Dhakal ◽  
M Bhatta ◽  
B K Tamrakar
Keyword(s):  
Polymerase Chain Reaction ◽  
Pleural Effusion ◽  
Adenosine Deaminase ◽  
Statistical Significance ◽  
Extrapulmonary Tuberculosis ◽  
Cross Sectional Study ◽  
Chain Reaction ◽  
Cross Sectional ◽  
Diagnosis And Prognosis ◽  
Polymerase Chain

Background: Extrapulmonary tuberculosis is one of the major causes of exudative pleural effusion. The paucibacillary nature of effusion suggests the need of markers and methodologies for accurate diagnosis and prognosis of tuberculosis as well as to differentiate it from other non-tubercular causes of pleural effusion.Objectives: This study was focused to evaluate the utility of polymerase chain reaction (PCR) in detection of tuberculosis antigen and to assess the level of Adenosine Deaminase (ADA) in tubercular pleural effusion (TPE) and contrast it with other causes.Methods: This is a cross-sectional study where 100 samples of pleural effusion suspected to be tuberculosis were analyzed by PCR for the detection of IS6110 segment of DNA. The level of ADA was then determined and compared in both PCR positive and negative samples. The cut-off value of ADA was >40 U/L for TPE. Student t test was applied to compare the means with statistical significance set at p<0.05.Results: Out of 100 samples analyzed, 45% were positive for TPE and remaining 55% were non-tuberculosis pleural effusion as detected by PCR. The level of ADA was above cut-off (>40 U/L) in 43% TPE samples whereas all the non-tuberculosis effusion had ADA <40 U/L (p<0.001).Conclusion: The ADA level was significantly higher in TPE than in non-tuberculosis cases, and PCR was able to detect suspected cases of tuberculosis effusion in almost half of the cases. This finding suggests the diagnostic utility of combined use of ADA and PCR in diagnosis of TPE. J-GMC-N | Volume 11 | Issue 01 | January-June 2018, Page: 5-9

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