Genetic and phenotypic characterization of indolent T-cell lymphoproliferative disorders of the gastrointestinal tract

The rate of detection of chromosome abnormalities in T-cell proliferations is lower than that observed in B-cell malignancies. The former frequently involve the TCRα/δ locus at chromosome band 14q11. We have identified a YAC encompassing 70% of the TCRα/δ locus, which has been used as a fluorescence in situ hybridization probe to detect chromosome rearrangements involving 14q11, both at metaphase and within interphase nuclei, in patients with a variety of T-lymphoproliferative disorders. Its use allowed detection of previously unsuspected TCRα/δ rearrangements in 4/13 (30%) immature T-lineage acute leukemias, including two t(10; 14) and 2 minor inversion 14s. It also clarified interpretation of complex chromosome 14 abnormalities in mature T-cell proliferations (T-prolymphocytic leukemia and ataxia telangiectasia). Use of this probe will aid the detection and characterization of abnormalities involving the TCRα/δ locus, particularly in cases with normal or complex karyotypes and in those proliferations for which mitoses are difficult to obtain.

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A Chromosome 14q11/TCRα/δ Specific Yeast Artificial Chromosome Improves the Detection Rate and Characterization of Chromosome Abnormalities in T-Lymphoproliferative Disorders

Blood ◽

10.1182/blood.v90.3.1233 ◽

1997 ◽

Vol 90 (3) ◽

pp. 1233-1240 ◽

Cited By ~ 9

Author(s):

K.A. Rack ◽

F. Cornélis ◽

I. Radford-Weiss ◽

A. Bernheim ◽

C.J. Harrison ◽

...

Keyword(s):

T Cell ◽

Yeast Artificial Chromosome ◽

Lymphoproliferative Disorders ◽

Chromosome Abnormalities ◽

Chromosome Band ◽

Hybridization Probe ◽

Acute Leukemias ◽

Prolymphocytic Leukemia ◽

Cell Proliferations

Abstract The rate of detection of chromosome abnormalities in T-cell proliferations is lower than that observed in B-cell malignancies. The former frequently involve the TCRα/δ locus at chromosome band 14q11. We have identified a YAC encompassing 70% of the TCRα/δ locus, which has been used as a fluorescence in situ hybridization probe to detect chromosome rearrangements involving 14q11, both at metaphase and within interphase nuclei, in patients with a variety of T-lymphoproliferative disorders. Its use allowed detection of previously unsuspected TCRα/δ rearrangements in 4/13 (30%) immature T-lineage acute leukemias, including two t(10; 14) and 2 minor inversion 14s. It also clarified interpretation of complex chromosome 14 abnormalities in mature T-cell proliferations (T-prolymphocytic leukemia and ataxia telangiectasia). Use of this probe will aid the detection and characterization of abnormalities involving the TCRα/δ locus, particularly in cases with normal or complex karyotypes and in those proliferations for which mitoses are difficult to obtain.

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Molecular and phenotypic characterization of an early T-cell precursor acute lymphoblastic lymphoma harboring PICALM-MLLT10 fusion with aberrant expression of B-cell antigens

Cancer Genetics ◽

10.1016/j.cancergen.2019.11.002 ◽

2020 ◽

Vol 240 ◽

pp. 40-44 ◽

Cited By ~ 2

Author(s):

Sharad Khurana ◽

Megan E. Melody ◽

Rhett P. Ketterling ◽

Jess F. Peterson ◽

Ivy M. Luoma ◽

...

Keyword(s):

T Cell ◽

B Cell ◽

Lymphoblastic Lymphoma ◽

Phenotypic Characterization ◽

Cell Precursor ◽

Aberrant Expression

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Indolent T-Cell Lymphoproliferative Disorders of the Gastrointestinal Tract (iTLPD-GI): A Review

Cancers ◽

10.3390/cancers13112790 ◽

2021 ◽

Vol 13 (11) ◽

pp. 2790

Author(s):

Francesca Sanguedolce ◽

Magda Zanelli ◽

Maurizio Zizzo ◽

Stefano Luminari ◽

Giovanni Martino ◽

...

Keyword(s):

Gastrointestinal Tract ◽

T Cell ◽

Treatment Options ◽

Lymphoproliferative Disease ◽

Lymphoproliferative Disorders ◽

Low Grade ◽

Diagnostic Biomarkers ◽

Molecular Features ◽

Uncommon Disease ◽

Neoplastic Disorders

iTLPD-GI is a low-grade clonal T-cell lymphoproliferative disease arising in GI organs. It is an uncommon disease, and only recently has it been enlisted as a distinct provisional entity in the current WHO Classification. Data from the literature disclose high heterogeneity in terms of pathological and molecular features; on the other hand, establishing an accurate diagnosis of iTLPD-GI is of pivotal importance, since treatment options are different from that of other, more frequent lymphomas that arise in the gastrointestinal tract. In this review, we aimed to better define this novel entity, and to identify useful diagnostic biomarkers; moreover, we provide a biomarker-based approach to the diagnosis and describe the most common issues in differentiating iTLPD-GI from other neoplastic and non-neoplastic disorders.

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