Biomimetic reduced graphene oxide coated collagen scaffold for in situ bone regeneration

AbstractA variety of bone-related diseases and injures and limitations of traditional regeneration methods require new tissue substitutes. Tissue engineering and regeneration combined with nanomedicine can provide different natural or synthetic and combined scaffolds with bone mimicking properties for implantation in the injured area. In this study, we synthesized collagen (Col) and reduced graphene oxide coated collagen (Col-rGO) scaffolds, and we evaluated their in vitro and in vivo effects on bone tissue repair. Col and Col-rGO scaffolds were synthesized by chemical crosslinking and freeze-drying methods. The surface topography, and the mechanical and chemical properties of scaffolds were characterized, showing three-dimensional (3D) porous scaffolds and successful coating of rGO on Col. The rGO coating enhanced the mechanical strength of Col-rGO scaffolds to a greater extent than Col scaffolds by 2.8 times. Furthermore, Col-rGO scaffolds confirmed that graphene addition induced no cytotoxic effects and enhanced the viability and proliferation of human bone marrow-derived mesenchymal stem cells (hBMSCs) with 3D adherence and expansion. Finally, scaffold implantation into rabbit cranial bone defects for 12 weeks showed increased bone formation, confirmed by Hematoxylin–Eosin (H&E) and alizarin red staining. Overall, the study showed that rGO coating improves Col scaffold properties and could be a promising implant for bone injuries.

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Graphene Coated Scaffold for Bone Tissue Engineering: Physicochemical and Osteogenic Characterizations

10.21203/rs.3.rs-408730/v1 ◽

2021 ◽

Author(s):

Sajad Bahrami ◽

Nafiseh Baheiraei ◽

Mostafa Shahrezaee

Keyword(s):

Tissue Engineering ◽

Bone Tissue ◽

Three Dimensional ◽

Chemical Properties ◽

Cranial Bone ◽

Drying Methods ◽

Porous Scaffolds ◽

Alizarin Red

Abstract Variety of bone-related diseases and injures and limitations of traditional regeneration methods need to introduce new tissue substitutes. Tissue engineering and regeneration combined with nanomedicine can provide different natural or synthetic and combined scaffolds with bone mimicking properties for implant in the injured area. In this study, we synthesized collagen (Col) and reduced graphene oxide coated collagen (Col-rGO) scaffolds and evaluated their in vitro and in vivo effects on bone tissue repair. Col and Col-rGO scaffolds were synthesized by chemical crosslinking and freeze-drying methods. The surface topography, mechanical and chemical properties of scaffolds were characterized and showed three-dimensional (3D) porous scaffolds and successful coating of rGO on Col. rGO coating enhanced mechanical strength of Col-rGO scaffolds compared with Col scaffolds by 2.8 folds. Furthermore, Col-rGO scaffolds confirmed that graphene addition not only did not any cytotoxic effects but also enhanced human bone marrow-derived mesenchymal stem cells (hBMSCs) viability and proliferation with 3D adherence and expansion. Finally, scaffolds implantation into rabbit cranial bone defect for 12 weeks showed increased bone formation, confirmed by Hematoxylin-Eosin (H&E) and alizarin red staining. Altogether, the study showed that rGO coating improves Col scaffold properties and could be a promising implant for bone injuries.

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In vitro and in vivo studies of electroactive reduced graphene oxide-modified nanofiber scaffolds for peripheral nerve regeneration

Acta Biomaterialia ◽

10.1016/j.actbio.2018.11.032 ◽

2019 ◽

Vol 84 ◽

pp. 98-113 ◽

Cited By ~ 46

Author(s):

Juan Wang ◽

Yuan Cheng ◽

Liang Chen ◽

Tonghe Zhu ◽

Kaiqiang Ye ◽

...

Keyword(s):

Graphene Oxide ◽

Peripheral Nerve ◽

Nerve Regeneration ◽

Reduced Graphene Oxide ◽

Peripheral Nerve Regeneration ◽

In Vivo Studies ◽

Reduced Graphene ◽

Nanofiber Scaffolds

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Neurotensin-Conjugated Reduced Graphene Oxide with Multi-Stage Near-Infrared-Triggered Synergic Targeted Neuron Gene Transfection In Vitro and In Vivo for Neurodegenerative Disease Therapy

Advanced Healthcare Materials ◽

10.1002/adhm.201600647 ◽

2016 ◽

Vol 5 (23) ◽

pp. 3016-3026 ◽

Cited By ~ 18

Author(s):

Tsung-Ying Hsieh ◽

Wei-Chen Huang ◽

Yi-Da Kang ◽

Chao-Yi Chu ◽

Wen-Lin Liao ◽

...

Keyword(s):

Graphene Oxide ◽

Neurodegenerative Disease ◽

Reduced Graphene Oxide ◽

Near Infrared ◽

Gene Transfection ◽

Multi Stage ◽

Disease Therapy ◽

Reduced Graphene

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Transdermal delivery of flurbiprofen from polyoxypropylene-polyoxyethylene block copolymer stabilized reduced graphene oxide to manage pain in spondylitis: In vitro and in vivo studies

European Journal of Pharmaceutical Sciences ◽

10.1016/j.ejps.2021.105929 ◽

2021 ◽

pp. 105929

Author(s):

Chaoqun Yang ◽

Tao Li

Keyword(s):

Graphene Oxide ◽

Block Copolymer ◽

Reduced Graphene Oxide ◽

Transdermal Delivery ◽

In Vivo Studies ◽

Reduced Graphene

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Heparin-reduced graphene oxide nanocomposites for curcumin delivery: in vitro, in vivo and molecular dynamics simulation study

Biomaterials Science ◽

10.1039/c8bm00907d ◽

2019 ◽

Vol 7 (3) ◽

pp. 1011-1027 ◽

Cited By ~ 14

Author(s):

Xiaoqun Shi ◽

Yang Wang ◽

Haiyan Sun ◽

Yujuan Chen ◽

Xingzhen Zhang ◽

...

Keyword(s):

Molecular Dynamics ◽

Graphene Oxide ◽

Molecular Dynamics Simulation ◽

Reduced Graphene Oxide ◽

Simulation Study ◽

Dynamics Simulation ◽

Reduced Graphene

We fabricated novel rGO-based nanocomposites and analyzed their interaction with drug and proteins via a molecular dynamics study.

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CdSe- Reduced graphene oxide nanocomposite toxicity alleviation via V2O5 shell formation over CdSe core: in vivo and in vitro studies

Nanotechnology ◽

10.1088/1361-6528/ab8b0f ◽

2020 ◽

Vol 31 (41) ◽

pp. 415101

Author(s):

Ashwani Kumar Singh ◽

Amar Nath Yadav ◽

Saurabh Srivastav ◽

Rishi Kumar Jaiswal ◽

Amit Srivastava ◽

...

Keyword(s):

Graphene Oxide ◽

Reduced Graphene Oxide ◽

In Vitro Studies ◽

Shell Formation ◽

Cdse Core ◽

Reduced Graphene

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Plasmonic photothermal cancer therapy with gold nanorods/reduced graphene oxide core/shell nanocomposites

RSC Advances ◽

10.1039/c5ra24662h ◽

2016 ◽

Vol 6 (2) ◽

pp. 1600-1610 ◽

Cited By ~ 32

Author(s):

Kostiantyn Turcheniuk ◽

Tetiana Dumych ◽

Rostyslav Bilyy ◽

Volodymyr Turcheniuk ◽

Julie Bouckaert ◽

...

Keyword(s):

Graphene Oxide ◽

Cancer Therapy ◽

Reduced Graphene Oxide ◽

Photon Energy ◽

Gold Nanorods ◽

Core Shell ◽

Efficient Conversion ◽

Reduced Graphene

Gold nanorods (Au NRs) are known for their efficient conversion of photon energy into heat, resulting in hyperthermia and suppression of tumor growths in vitro and in vivo.

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In vitro and in vivo effects of graphene oxide and reduced graphene oxide on glioblastoma

International Journal of Nanomedicine ◽

10.2147/ijn.s77591 ◽

2015 ◽

pp. 1585 ◽

Cited By ~ 11

Author(s):

André Chwalibog ◽

Slawomir Jaworski ◽

Ewa Sawosz ◽

Marta Kutwin ◽

Mateusz Wierzbicki ◽

...

Keyword(s):

Graphene Oxide ◽

Reduced Graphene Oxide ◽

Reduced Graphene ◽

In Vivo Effects

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Gelatin reduced Graphene Oxide Nanosheets as Kartogenin Nanocarrier Induces Rat ADSCs Chondrogenic Differentiation Combining with Autophagy Modification

Materials ◽

10.3390/ma14051053 ◽

2021 ◽

Vol 14 (5) ◽

pp. 1053

Author(s):

Delong Jiao ◽

Jing Wang ◽

Wenting Yu ◽

Ning Zhang ◽

Ke Zhang ◽

...

Keyword(s):

Stem Cells ◽

Graphene Oxide ◽

Reduced Graphene Oxide ◽

Chondrogenic Differentiation ◽

Drug Loading ◽

Pcr Analysis ◽

Promoting Effect ◽

Reduced Graphene

Biocompatible reduced graphene oxide (rGO) could deliver drugs for synergistically stimulating stem cells directed differentiation with influences on specific cellular activities. Here, we prepared a biodegradable gelatin reduced graphene oxide (rGO@Ge) to evaluate its functions in promoting rat adipose derived mesenchymal stem cells (ADSCs) chondrogenic differentiation through delivering kartogenin (KGN) into the stem cell efficiently. The optimum KGN concentration (approximately 1 μM) that promoted the proliferation and chondrogenic differentiation of ADSCs was clarified by a series of experiments, including immunofluorescent (IF) staining (Sox-9, Col II), alcian blue (Ab) staining, toluidine blue (Tb) staining and real-time quantitative PCR analysis of the chondrogenic markers. Meanwhile, the biocompatibility of rGO@Ge was evaluated to clearly define the nonhazardous concentration range, and the drug loading and releasing properties of rGO@Ge were tested with KGN for its further application in inducing ADSCs chondrogenic differentiation. Furthermore, the mechanism of rGO@Ge entering ADSCs was investigated by the different inhibitors that are involved in the endocytosis of the nanocarrier, and the degradation of the rGO@Ge in ADSCs was observed by transmission electron microscopy (TEM). The synergistic promoting effect of rGO@Ge nanocarrier on ADSCs chondrogenesis with KGN was also studied by the IF, Ab, Tb stainings and PCR analysis of the chondrogenic markers. Finally, the intracellular Reactive Oxygen Species (ROS) and autophagy induced by KGN/rGO@Ge complex composites were tested in details for clarification on the correlation between the autophagy and chondrogenesis in ADSCs induced by rGO@Ge. All the results show that rGO@Ge as a biocompatible nanocarrier can deliver KGN into ADSCs for exerting a pro-chondrogenic effect and assist the drug to promote ADSCs chondrogenesis synergistically through modification of the autophagy in vitro, which promised its further application in repairing cartilage defect in vivo.

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