scholarly journals Antimicrobial Effect of Roselle (Hibiscus sabdariffa L.) Water Fraction against Pseudomonas aeruginosa using Drosophila Infection Model

2021 ◽  
Vol 11 (5) ◽  
pp. 12877-12885
Keyword(s):  
Gene Expression ◽  
Pseudomonas Aeruginosa ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Mrna Levels ◽  
Antibacterial Compounds ◽  
Water Fraction ◽  
Survival Assay ◽  
The Impact

Pseudomonas aeruginosa is one of the most common pathogenic bacteria that cause nosocomial infection. Unfortunately, the irrational use of antibiotics has created a surge in P. aeruginosa resistance nowadays. To overcome this situation, new antibacterial compounds are urgently needed. One of the potential sources to obtain such antibacterial compounds is roselle calyx. This research was carried out using two experimental approaches, survival assay and gene expression analysis, to examine the in vivo antibacterial effect of water fraction of roselle calyx (WFR) against Pseudomonas aeruginosa in Drosophila model of infection. Survival assay was used to demonstrate the impact of treatment on the lifespan of the infected host. The measurement of immune-related Dpt mRNA levels by reverse-transcriptase quantitative PCR (RT-qPCR) was used to assess whether immunostimulation is involved in the antibacterial protection of WFR against P. aeruginosa. The result demonstrated that WFR at concentrations of 0.8% and 2% were able to enhance P. aeruginosa-infected flies' survival. Furthermore, gene expression analysis showed the insignificant difference between WFR-treated flies and healthy control flies at all tested concentrations, implying the non-involvement of Imd-Dpt-mediated pathway immunity in the antipseudomonal protection of WFR. Taken together, our data suggested the in vivo antibacterial activity of WFR against P. aeruginosa in the fruit fly model of infection.

Gene Expression Analysis of Troglitazone Reveals Its Impact on Multiple Pathways in Cell Culture: A Case for In Vitro Platforms Combined with Gene Expression Analysis for Early (Idiosyncratic) Toxicity Screening

2006 ◽  
Vol 25 (2) ◽  
pp. 85-94 ◽  
Author(s):  
Gordon Vansant ◽  
Patrick Pezzoli ◽  
Robert Saiz ◽  
Aaron Birch ◽  
Chris Duffy ◽  
...  
Keyword(s):  
Gene Expression ◽  
Type 2 Diabetes ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Peroxisome Proliferator ◽  
Toxicity Screening ◽  
The Impact

Peroxisome proliferator-activated receptor gamma (PPAR γ) agonists of the thiazolidinedione family are used for the treatment of type 2 diabetes mellitus due to their ability to reduce glucose and lipid levels in patients with this disease. Three thiazolidinediones that were approved for treatment are Rezulin (troglitazone), Avandia (rosiglitazone), and Actos (pioglitazone). Troglitazone was withdrawn from the market due to idiosyncratic drug toxicity. Rosiglitazone and pioglitazone are still on the market for the treatment of type 2 diabetes. The authors present data from a gene expression screen that compares the impact these three compounds have in rats, in rat hepatocytes, and in the clone 9 rat liver cell line. The authors monitored the changes in expression in multiple genes, including those related to xenobiotic metabolism, proliferation, DNA damage, oxidative stress, apoptosis, and inflammation. Compared to the other two compounds, troglitazone had a significant impact on many of the pathways monitored in vitro although no major perturbation was detected in vivo. The changes detected predict not only general toxicity but potential mechanisms of toxicity. Based on gene expression analysis, the authors propose there is not just one but multiple ways troglitazone could be toxic, depending on a patient’s environment and genetic makeup, including immune response-related toxicity.

scholarly journals Effects of Vitrification on the Blastocyst Gene Expression Profile in a Porcine Model

2021 ◽  
Vol 22 (3) ◽  
pp. 1222
Author(s):  
Cristina Cuello ◽  
Cristina A. Martinez ◽  
Josep M. Cambra ◽  
Inmaculada Parrilla ◽  
Heriberto Rodriguez-Martinez ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Analysis ◽  
Survival Rates ◽  
Control Group ◽  
P Value ◽  
Transcriptome Profile ◽  
Embryo Viability ◽  
The Impact

This study was designed to investigate the impact of vitrification on the transcriptome profile of blastocysts using a porcine (Sus scrofa) model and a microarray approach. Blastocysts were collected from weaned sows (n = 13). A total of 60 blastocysts were vitrified (treatment group). After warming, vitrified embryos were cultured in vitro for 24 h. Non-vitrified blastocysts (n = 40) were used as controls. After the in vitro culture period, the embryo viability was morphologically assessed. A total of 30 viable embryos per group (three pools of 10 from 4 different donors each) were subjected to gene expression analysis. A fold change cut-off of ±1.5 and a restrictive threshold at p-value < 0.05 were used to distinguish differentially expressed genes (DEGs). The survival rates of vitrified/warmed blastocysts were similar to those of the control (nearly 100%, n.s.). A total of 205 (112 upregulated and 93 downregulated) were identified in the vitrified blastocysts compared to the control group. The vitrification/warming impact was moderate, and it was mainly related to the pathways of cell cycle, cellular senescence, gap junction, and signaling for TFGβ, p53, Fox, and MAPK. In conclusion, vitrification modified the transcriptome of in vivo-derived porcine blastocysts, resulting in minor gene expression changes.

Assessing the Impact of Tissue Devitalization Time on Genome-wide Gene Expression Analysis in Ovarian Tumor Samples

2008 ◽  
Vol 17 (4) ◽  
pp. 200-206 ◽  
Author(s):  
Catherine I. Dumur ◽  
Sherjeel Sana ◽  
Amy C. Ladd ◽  
Andrea Ferreira-Gonzalez ◽  
David S. Wilkinson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Ovarian Tumor ◽  
Gene Expression Analysis ◽  
Genome Wide ◽  
The Impact ◽  
Genome Wide Gene Expression

Global Gene Expression Analysis of Long and Short Day 15 Bovine Conceptuses Produced In Vivo.

2012 ◽  
Vol 87 (Suppl_1) ◽  
pp. 192-192
Author(s):  
Callie V. Barnwell ◽  
Christopher M. Ashwell ◽  
Peter W. Farin ◽  
William T. Farmer ◽  
Charlotte E. Farin
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Global Gene Expression ◽  
Short Day ◽  
Global Gene Expression Analysis

375 Gene expression analysis of galactosamine-induced hepatotoxicity in-vivo and in-vitro

2003 ◽  
Vol 144 ◽  
pp. s102
Author(s):  
H. Hildebrand ◽  
G. Kempka ◽  
H. Ellinger ◽  
B. Stuart ◽  
B. Wahle ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Gene Expression Analysis

scholarly journals New Insights about Antibiotic Production by Pseudomonas aeruginosa: A Gene Expression Analysis

2017 ◽  
Vol 5 ◽  
Author(s):  
Bárbara Gionco ◽  
Eliandro R. Tavares ◽  
Admilton G. de Oliveira ◽  
Sueli F. Yamada-Ogatta ◽  
Anderson O. do Carmo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Pseudomonas Aeruginosa ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Antibiotic Production

scholarly journals Toxicological and gene expression analysis of the impact of aflatoxin B1 on hepatic function of male broiler chicks

2009 ◽  
Vol 88 (2) ◽  
pp. 360-371 ◽  
Author(s):  
L.P. Yarru ◽  
R.S. Settivari ◽  
E. Antoniou ◽  
D.R. Ledoux ◽  
G.E. Rottinghaus
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Aflatoxin B1 ◽  
Gene Expression Analysis ◽  
Hepatic Function ◽  
Broiler Chicks ◽  
The Impact

Role of the snake venom toxin jararhagin in proinflammatory pathogenesis: In vitro and in vivo gene expression analysis of the effects of the toxin

2005 ◽  
Vol 441 (1) ◽  
pp. 1-15 ◽  
Author(s):  
Paul Gallagher ◽  
Yongde Bao ◽  
Solange M.T. Serrano ◽  
Gavin D. Laing ◽  
R. David G. Theakston ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Snake Venom ◽  
Gene Expression Analysis ◽  
Venom Toxin ◽  
Snake Venom Toxin

Human and murine amniotic fluid c-Kit+Lin− cells display hematopoietic activity

Blood ◽  
2009 ◽  
Vol 113 (17) ◽  
pp. 3953-3960 ◽  
Author(s):  
Andrea Ditadi ◽  
Paolo de Coppi ◽  
Olivier Picone ◽  
Laetitia Gautreau ◽  
Rim Smati ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Amniotic Fluid ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Lymphoid Cells ◽  
Immunocompromised Hosts ◽  
Hematopoietic Activity

Abstract We have isolated c-Kit+Lin− cells from both human and murine amniotic fluid (AF) and investigated their hematopoietic potential. In vitro, the c-Kit+Lin− population in both species displayed a multilineage hematopoietic potential, as demonstrated by the generation of erythroid, myeloid, and lymphoid cells. In vivo, cells belonging to all 3 hematopoietic lineages were found after primary and secondary transplantation of murine c-Kit+Lin− cells into immunocompromised hosts, thus demonstrating the ability of these cells to self-renew. Gene expression analysis of c-Kit+ cells isolated from murine AF confirmed these results. The presence of cells with similar characteristics in the surrounding amnion indicates the possible origin of AF c-Kit+Lin− cells. This is the first report showing that cells isolated from the AF do have hematopoietic potential; our results support the idea that AF may be a new source of stem cells for therapeutic applications.

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