Predictive Value of NKILA and lnc13 lnc RNAs for Distinguishing of Celiac Disease and IBD: A Case-Control Study

Long-noncoding RNA 13 (lnc13) and NF-κB-interacting lncRNA (NKILA) are long noncoding RNAs (lncRNAs) that play a role in inflammatory disorders pathogenesis. This study aimed at investigating the expression of these lncRNAs in inflammatory bowel disease (IBD) and celiac disease (CeD) patients compared to controls. We evaluated mRNA expression of lnc13 and NKILA in peripheral blood and intestinal biopsy samples of 50 CeD, 46 IBD patients, and 20 controls using qRT- PCR method. Compared to the controls, CeD and IBD patients had a significantly higher PBMC mRNA level of Lnc13 (p < 0.0001 for both of them) and NKILA (p < 0.0001 and p=0.0174, respectively). NKILA mRNA level was significantly higher in CeD than in IBD patients (p < 0.0001). IBD subjects had significantly increased colonic NKILA expression (p=0.004). In UC, NKILA, and CD, lnc13 colonic expression was significantly increased compared to others (p=0.002 and p=0.0111 respectively for UC, p = 0.0075 and p = 0.002, respectively for CD). Collectively, increased peripheral expression of lnc13 and NKILA along with their decreased duodenal expression demonstrates CeD. Moreover, suppose the increased peripheral expression of lnc13 and NKILA is associated with increased lnc13 colonic expression. In that case, it indicates CD, and if it is associated with increased NKILA colonic expression, it indicates UC.

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Associated factors for celiac disease, and utility of tissue transglutaminase antibody tests for diagnosis: a matched case-control study

10.21203/rs.3.rs-918587/v1 ◽

2021 ◽

Author(s):

Eman Jafar Al Alawi ◽

Nagarajkumar Yenugadhati ◽

Sundas Javad ◽

Hamdan Al Jahdli ◽

Motasim Badri

Keyword(s):

Celiac Disease ◽

Case Control Study ◽

Tissue Transglutaminase ◽

Public Awareness ◽

Conditional Logistic Regression ◽

Case Control ◽

Screening Tests ◽

Intestinal Biopsy ◽

Awareness Campaigns ◽

Control Study

Abstract Background Prevalence of Celiac disease (CD) increased worldwide in recent decades. Approximately 53% of the 33.4 million Saudi population are genetically susceptible and 1.5% develop CD. This underscores the importance of identification of factors associated with CD and the diagnostic accuracy of CD screening tests. Methods In this case-control study records of patients managed between January 2016 and July 2019 at Gastroenterology Department, King Abdul-Aziz Medical City, Riyadh, Saudi Arabia were reviewed. CD cases with confirmed intestinal biopsy were matched (1:1) by age with biopsy negative controls free of CD. Area under the curve (AUC) for anti-tissue transglutaminase IgA (TTG-IgA) and IgG (TTG-IgG) CD diagnostic tests were calculated and compared. Results The study included 270 cases and 270 controls. Sex distribution was similar in both groups. In a stepwise conditional logistic regression analysis, factors significantly associated with CD were family history (odds ratio (OR)=7.76, 95% confidence interval (CI): 2.26-26.63, P=0.001), Helicobacter pylori infection (OR=1.72, 95% CI: 1.10-2.71, P=0.018), diabetes mellitus (OR=3.37, 95% CI: 1.68-6.74, P=0.001), hypothyroidism (OR=2.46, 95% CI: 1.15-5.28, P=0.02) and respiratory infections (OR=4.89,95% CI 2.26-10.56, P<0.001). AUC for TTG-IgA test was 0.934 and for TTG-IgG was 0.787, P<0.001. The optimal cut-off for TTG-IgA was >=12.7 U/ml, with 89% sensitivity and 86% specificity, and for TTG-IgG was >=3.5 U/ml, with 70% sensitivity and 77% specificity.Conclusion The findings of this study can inform strategies for CD screening and prevention. Public awareness campaigns for CD are urgently needed, particularly, for high-risk groups.

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Inflammatory bowel disease in sub-Saharan Africa: a protocol of a prospective registry with a nested case–control study

BMJ Open ◽

10.1136/bmjopen-2020-039456 ◽

2020 ◽

Vol 10 (12) ◽

pp. e039456

Author(s):

Leolin Katsidzira ◽

Wisdom F Mudombi ◽

Rudo Makunike-Mutasa ◽

Bahtiyar Yilmaz ◽

Annika Blank ◽

...

Keyword(s):

Risk Factors ◽

Inflammatory Bowel Disease ◽

Case Control Study ◽

Alpha Diversity ◽

Case Control ◽

Sub Saharan Africa ◽

Inflammatory Bowel ◽

Sub Saharan ◽

Nested Case Control ◽

Control Study

IntroductionThe epidemiology of inflammatory bowel disease (IBD) in sub-Saharan Africa is poorly documented. We have started a registry to determine the burden, phenotype, risk factors, disease course and outcomes of IBD in Zimbabwe.Methods and analysisA prospective observational registry with a nested case–control study has been established at a tertiary hospital in Harare, Zimbabwe. The registry is recruiting confirmed IBD cases from the hospital, and other facilities throughout Zimbabwe. Demographic and clinical data are obtained at baseline, 6 months and annually. Two age and sex-matched non-IBD controls per case are recruited—a sibling or second-degree relative, and a randomly selected individual from the same neighbourhood. Cases and controls are interviewed for potential risk factors of IBD, and dietary intake using a food frequency questionnaire. Stool is collected for 16S rRNA-based microbiota profiling, and along with germline DNA from peripheral blood, is being biobanked. The estimated sample size is 86 cases and 172 controls, and the overall registry is anticipated to run for at least 5 years. Descriptive statistics will be used to describe the demographic and phenotypic characteristics of IBD, and incidence and prevalence will be estimated for Harare. Risk factors for IBD will be analysed using conditional logistic regression. For microbial analysis, alpha diversity and beta diversity will be compared between cases and controls, and between IBD phenotypes. Mann-Whitney U tests for alpha diversity and Adonis (Permutational Multivariate Analysis of Variance) for beta diversity will be computed.Ethics and disseminationEthical approval has been obtained from the Parirenyatwa Hospital’s and University of Zimbabwe’s research ethics committee and the Medical Research Council of Zimbabwe. Findings will be discussed with patients, and the Zimbabwean Ministry of Health. Results will be presented at scientific meetings, published in peer reviewed journals, and on social media.Trial registration numberNCT04178408.

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Mo1999 – Prevalence of Celiac Disease (CD) in Subjects with Dyspeptic Symptoms. A Case-Control Study

Gastroenterology ◽

10.1016/s0016-5085(19)39262-5 ◽

2019 ◽

Vol 156 (6) ◽

pp. S-918

Author(s):

Jahaziel Lara-Carmona ◽

Mercedes Amieva-Balmori ◽

Arturo Martínez-Conejo ◽

Francisco Javier Cabrera Jorge ◽

Karla Rocío García-Zermeño ◽

...

Keyword(s):

Celiac Disease ◽

Case Control Study ◽

Case Control ◽

Dyspeptic Symptoms ◽

Control Study

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Premedication Does Not Influence the Incidence of Infliximab Infusion Reactions in Pediatric Patients with Inflammatory Bowel Disease—A Single Center Case–Control Study

Journal of Clinical Medicine ◽

10.3390/jcm10143177 ◽

2021 ◽

Vol 10 (14) ◽

pp. 3177

Author(s):

Edyta Szymanska ◽

Maciej Dadalski ◽

Joanna Sieczkowska-Golub ◽

Dorota Jarzebicka ◽

Monika Meglicka ◽

...

Keyword(s):

Inflammatory Bowel Disease ◽

Bowel Disease ◽

Pediatric Patients ◽

Case Control Study ◽

Case Control ◽

Chi Square ◽

Infusion Reactions ◽

Inflammatory Bowel ◽

The Difference ◽

Control Study

Background: Infusion reactions (IRs) are the most common adverse events (AEs) of infliximab (IFX) treatment in patients with inflammatory bowel disease (IBD). Prophylactic premedication (PM) with corticosteroids or antihistamines prior to IFX infusions has been used in clinical practice, but its efficacy is not known. The aim of this study was to assess the influence of steroid PM on IR incidence in pediatric patients with IBD receiving IFX. Methods: We performed a case–control study that included pediatric patients with IBD receiving IFX. Patients were divided into four subgroups according to the agent and PM they received: Remicade (original drug) + PM, and two biosimilars—Reshma +/− PM, and Flixabi—PM. At our site, until 2018, PM with steroids was used as a part of standard IFX infusion (PM+); however, since then, this method has no longer been administered (PM−). IRs were divided into mild/severe reactions. Differences between subgroups were assessed with the appropriate chi-square test. Multivariate logistic regression was used to assess associations between PM and IR incidence, correcting for co-medication usage. Results: There were 105 children (55 PM+, 44 male, mean age 15 years) included in the study who received 1276 infusions. There was no difference between the PM+ and PM− subgroups, either in incidence of IR (18.2% vs. 16.0% of patients, p > 0.05) or in percentage of infusions followed by IR (2.02% vs. 1.02% of infusions, p > 0.5). The OR of developing IR when using PM was 0.34, and the difference in IRs ratio in PM+ and PM− patients was not statistically significant (95% CI, 0.034–1.9). There were 11/18 (61.1%) severe IRs (anaphylactic shock) reported in all patients (both PM+ and PM−). Conclusion: At our site, the incidence of IR was low, and PM did not decrease the incidence of IR in pediatric patients with IBD receiving IFX. These results indicate that PM with steroids should not be a standard part of IFX infusion to prevent IR.

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Tolerability of Bowel Preparation and Colonoscopy in IBD Patients: Results From a Prospective, Single-Center, Case–Control Study

Crohn's & Colitis 360 ◽

10.1093/crocol/otaa077 ◽

2020 ◽

Vol 2 (4) ◽

Author(s):

Cristina Bezzio ◽

Mario Schettino ◽

Gianpiero Manes ◽

Paolo Andreozzi ◽

Ilaria Arena ◽

...

Keyword(s):

Bowel Preparation ◽

Analogue Scale ◽

Case Control Study ◽

Screening Program ◽

Case Control ◽

Single Center ◽

Split Dose ◽

Inflammatory Bowel ◽

Control Study ◽

Demographic Features

Abstract Background Endoscopy plays a fundamental role in the management of patients with inflammatory bowel disease (IBD). The aim of this study was to prospectively evaluate the tolerability and efficacy of bowel preparation and colonoscopy in ulcerative colitis (UC) and Crohn’s disease (CD) patients compared to subjects participating in a colorectal cancer population screening program. Methods Consecutive enrolment of CD and UC patients and screening subjects (SS) undergoing colonoscopy. Bowel preparation was done by split dose of 2 L PEG-ELS + simethicone. We recorded endoscopic, clinical, and demographic features; cleanliness rating using the Boston Bowel Preparation Scale (BBPS); and sedation doses. Bowel-preparation tolerability, discomfort, and pain during colonoscopy were assessed using a Visual Analogue Scale from 0 to 100 mm. Results Sixty-three UC (mean age 49.9 ± 14.9 years), 63 CD (mean age 44.0 ± 14.0 years), and 63 SS (mean age 59.9 ± 6.3 years) patients were enrolled. Bowel preparation was similarly tolerated in UC, CD, and SS (P = 0.397). A complete colonoscopy was similarly performed in UC (59/63, 93.7%), CD (58/63, 92.1%), and SS (60/63, 95.2%) (P = 0.364). The BBPS did not show significant differences between UC (6.2 ± 1.6), CD (6.1 ± 1.3), and SS (6.2 ± 1.4) (P = 0.824). The need to increase sedation doses was significantly higher in CD (24/63, 38.1%) and UC (16/63, 25.4%) than in SS (4/63, 6.3%) (P < 0.0001). Conclusions Bowel preparation is equally tolerated and efficacious in IBD patients and in healthy SS. In IBD, higher sedation doses are needed to guarantee an equally tolerated colonoscopy.

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