scholarly journals Characterization and antibiogram profile of bacteria isolated from Buriganga river

2020 ◽  
Vol 49 (3) ◽  
pp. 451-457
Author(s):  
Md Abdul Karim ◽  
Rehena Nasrin Happy
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Amyloliquefaciens ◽  
Subtilis Strain ◽  
Strain Atcc ◽  
Bacterial Isolates ◽  
Biochemical Characteristics ◽  
Major Cluster ◽  
Bacillus Tequilensis ◽  
Subtilis Subsp ◽  
Buriganga River

Selected bacterial isolates from the surface water of Buriganga river were characterized by morphological, biochemical characteristics and sequence-based PCR-amplified fragments of 16S rRNA. All isolates were rod shaped and Gram-positive. The isolates were confirmed as Chryseobacterium arthrosphaerae strain FDAARGOS 519, Bacillus cabrialesii strain TE 3, Bacillus tequilensis KCTC strain 13622(T), Bacillus amyloliquefaciens DSM 7 strain ATCC 23350, Bacillus subtilis strain E20 and Bacillus subtilis subsp. subtilis strain 168 based on sequence analysis. A phylogenetic tree was constructed that showed only one major cluster comprising of two sub-clusters grouping Chryseobacterium arthrosphaerae in one and Bacillus tequilensis in another. Chryseobacterium arthrosphaerae strain FDAARGOS 519 was susceptible to all antibiotics at different ranges, while Bacillus tequilensis KCTC strain 13622(T) and Bacillus amyloliquefaciens DSM 7 strain ATCC 23350 were found to be resistant to polymyxin only but sensitive to other antibiotics. However, Bacillus cabrialesii strain TE 3 was resistant to polymyxin and neomycin, while Bacillus subtilis subsp. subtilis strain 168 was resistant to polymyxin, vancomycin and rifampicin.

scholarly journals Complete Genome Sequence of Bacillus subtilis subsp. subtilis Strain ∆6

2016 ◽  
Vol 4 (4) ◽  
Author(s):  
Daniel R. Reuß ◽  
Andrea Thürmer ◽  
Rolf Daniel ◽  
Wim J. Quax ◽  
Jörg Stülke
Keyword(s):  
Bacillus Subtilis ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Full Genome Sequence ◽  
Subtilis Strain ◽  
Full Genome ◽  
Biotechnological Applications ◽  
A Genome ◽  
Subtilis Subsp

Bacillus subtilis ∆6 is a genome-reduced strain that was cured from six prophages and AT-rich islands. This strain is of great interest for biotechnological applications. Here, we announce the full-genome sequence of this strain. Interestingly, the conjugative element ICE Bs 1 has most likely undergone self-excision in B. subtilis ∆6.

scholarly journals kinA mRNA is missing a stop codon in the undomesticated Bacillus subtilis strain ATCC 6051

Microbiology ◽  
2008 ◽  
Vol 154 (1) ◽  
pp. 54-63 ◽  
Author(s):  
Kazuo Kobayashi ◽  
Ritsuko Kuwana ◽  
Hiromu Takamatsu
Keyword(s):  
Bacillus Subtilis ◽  
Stop Codon ◽  
Subtilis Strain ◽  
Strain Atcc

scholarly journals Characterisation of bacteria isolated from the stingless bee, Heterotrigona itama, honey, bee bread and propolis

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7478 ◽  
Author(s):  
Mohamad Syazwan Ngalimat ◽  
Raja Noor Zaliha Raja Abd. Rahman ◽  
Mohd Termizi Yusof ◽  
Amir Syahir ◽  
Suriana Sabri
Keyword(s):  
Bacillus Subtilis ◽  
Honey Bee ◽  
Bacillus Amyloliquefaciens ◽  
Bacterial Species ◽  
Stingless Bee ◽  
Plate Count ◽  
Bacterial Isolates ◽  
Phenotypic Profile ◽  
Bacillus Safensis ◽  
Bee Bread

Bacteria are present in stingless bee nest products. However, detailed information on their characteristics is scarce. Thus, this study aims to investigate the characteristics of bacterial species isolated from Malaysian stingless bee, Heterotrigona itama, nest products. Honey, bee bread and propolis were collected aseptically from four geographical localities of Malaysia. Total plate count (TPC), bacterial identification, phenotypic profile and enzymatic and antibacterial activities were studied. The results indicated that the number of TPC varies from one location to another. A total of 41 different bacterial isolates from the phyla Firmicutes, Proteobacteria and Actinobacteria were identified. Bacillus species were the major bacteria found. Therein, Bacillus cereus was the most frequently isolated species followed by Bacillus aryabhattai, Bacillus oleronius, Bacillus stratosphericus, Bacillus altitudinis, Bacillus amyloliquefaciens, Bacillus nealsonii, Bacillus toyonensis, Bacillus subtilis, Bacillus safensis, Bacillus pseudomycoides, Enterobacter asburiae, Enterobacter cloacae, Pantoea dispersa and Streptomyces kunmingensis. Phenotypic profile of 15 bacterial isolates using GEN III MicroPlate™ system revealed most of the isolates as capable to utilise carbohydrates as well as amino acids and carboxylic acids and derivatives. Proteolytic, lipolytic and cellulolytic activities as determined by enzymatic assays were detected in Bacillus stratosphericus PD6, Bacillus amyloliquefaciens PD9, Bacillus subtilis BD3 and Bacillus safensis BD9. Bacillus amyloliquefaciens PD9 showed broad-spectrum of antimicrobial activity against Gram-positive and Gram-negative bacteria in vitro. The multienzymes and antimicrobial activities exhibited by the bacterial isolates from H. itama nest products could provide potential sources of enzymes and antimicrobial compounds for biotechnological applications.

scholarly journals Study Biodegradation of Aromatics Pyrene Using Bacterial Isolates from the Sea and micro symbionts Sponges

2018 ◽  
Author(s):  
Ismail Marzuki ◽  
Hadija Enryani Ismai ◽  
Nursiah La Nafie
Keyword(s):  
Bacillus Subtilis ◽  
Degradation Rate ◽  
Carbon Sources ◽  
Alcaligenes Faecalis ◽  
Subtilis Strain ◽  
Bacterial Isolates ◽  
Biodegradation Rate ◽  
Specific Mechanism ◽  
Degradation Ability ◽  
Sponge Symbionts

Investigation of PAHs reshuffle by microorganisms has been done, but using sponge symbionts for the purpose of reduction of PAHs toxicity into simple organic compounds is still small. Microorganisms and sponges are known as complex animals that have various functions including the ability to produce bioactive compounds that can interact with hydrocarbon compounds as carbon sources. The purpose of this experiment was the use of bacteria isolated from seawater and sponges to reduce pyrene by biodegradation method by interacting between bacteria Alcaligenes faecalis strain Cu4-1 (AF), Bacillus subtilis strain BAB-1684 (BS) and Bacillus Cereus strain MER-8 (BC) capable of producing enzymes to overhaul the pyrene structure acting as a substrate. Biodegradation was carried out for 25 days, every 5 days to check degradation media using spectrophotometer and GC-MS to analyze degradation parameters: (1) optical density, (2) component abundance, (3) concentration and degradation rate of pyrene, and (4) ) Degraded organic of components. The results of the analysis of each degradation parameter were concluded: isolates, AF, BS and BC were able to degrade pyrene compounds, biodegradation rate: AF (97, 65%) ˃ BC (95, 27%) ˃ BS (93, 15%). Marine-filled bacteria (AF and BC) have a relatively higher pyrene degradation ability than the BS microsimbiont isolated on Callyspongi sp. Biodegradation of organic components results isolates AF and BC is different from the organic component isolates BS biodegradation results indicate that there is a specific mechanism which is owned by each type of bacteria to degrade PAHs

scholarly journals Complete Genome Sequence of Bacillus subtilis subsp. subtilis Strain 3NA

2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Daniel R. Reuß ◽  
Jörg Schuldes ◽  
Rolf Daniel ◽  
Josef Altenbuchner
Keyword(s):  
Bacillus Subtilis ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Subtilis Strain ◽  
Subtilis Subsp
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