Comparative Studies on Production of Cell Wall-Degrading Hydrolases by <i>Trichoderma reesei</i> and <i>T. viride</i> in Submerged and Solid-State Cultivations

Lignocellulose-degrading organisms have been used for the conversion of lignocellulosic materials into soluble sugars or solvents in several biotechnological and industrial applications. Complete degradation of complex highly structured lignocellosics requires a concerted action of a wide array hydrolytic enzymes. In this study, two newly isolated fungi, Trichoderma reesei and T. viride, were examined for their ability to produce cellulolytic, xylanolytic and pectinolytic enzymes in submerged and solid-state fermentations. The fungi produced appreciable amounts of the enzymes when grown on potentially low cost lignocellulosic agricultural residues like wheat bran, sugar cane bagasse and corncobs. T. viride seems to be superior to T. reesei with respect to enzyme productions both in submerged and solid-state cultivations. There was a distinct influence of culture methods on the production of the enzymes by the fungi. The enzyme productions were higher in solid-state fermentations than in submerged fermentations. However, taking into consideration of enzyme yields per gram substrate, it was found that the yields were many-fold higher in submerged cultures than in solid-state fermentations. The recovery of the enzymes from fermented slurries in solidstate fermentations was enhanced by using non-ionic surfactant Tween 80 as leaching agent. The enzymes produced by the fungi displayed optimum activities at pH range between 4.5 and 5.5, and at temperatures between 50 and 55°C. The fungi merit further attention as potential sources of industrial enzymes, as they exhibited some excellent properties including the ability to synthesize a wide array of hydrolytic enzymes while grown on cheap and readily available lignocellulosic residues. Keywords: Lignocellulosics, cellulase, xylanase, pectinase, Trichoderma reesei, Trichoderma viride, submerged culture, solid-state fermentationDOI: http://dx.doi.org/10.3329/bjm.v23i2.882 Bangladesh J Microbiol, Volume 23, Number 2, December 2006, pp 149-155

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Solid-state cultivation of Aspergillus niger–Trichoderma reesei from sugarcane bagasse with vinasse in bench packed-bed column bioreactor

10.21203/rs.3.rs-158166/v1 ◽

2021 ◽

Author(s):

Laura Macedo Rocha ◽

Beatriz Silva Campanhol ◽

Reinaldo Gaspar Bastos

Keyword(s):

Citric Acid ◽

Solid State ◽

Aspergillus Niger ◽

Trichoderma Reesei ◽

Sugarcane Bagasse ◽

Maximum Yield ◽

Hydrolytic Enzymes ◽

Packed Bed ◽

Industrial Applications ◽

Acid Yield

Abstract Solid-state cultivation (SSC) is microbial growth on solid supports under limited water conditions. Citric acid, one of the products obtained by SSC, is a microbial aerobic metabolic product with various industrial applications. Several wastes from agro-industries are used in SSC, such as sugarcane bagasse and vinasse. As xylanolytic enzymes of inoculum breakdown the lignocellulosic material (bagasse), mixed fungal cultures or co-cultures are used in these SSC. Thus, this study aims to evaluate the effect of inoculum (Aspergillus niger and Trichoderma reesei consortium) in the production of citric acid from sugarcane bagasse impregnated with vinasse using bench packed bed reactors (PBR). The results show the importance of T. reesei in inoculum with A. niger at a ratio of 50:50 and 25:75, suggesting the use of solid support due to the complementation of the hydrolytic enzymes. The highest concentration of approximately 1000 mg L− 1 of citric acid yield for 100 mm of bed height in 48 and 72 h, with the maximum yield from glucose to citric acid (2.2 mg citric acid mg glucose−1). kLa indicates that maintaining solid moisture and liquid film thickness is important to keep the oxygen transfer in SSC.

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Solid State Fermentation Parameters Effect on Cellulase Production from Empty Fruit Bunch

BULLETIN OF CHEMICAL REACTION ENGINEERING AND CATALYSIS ◽

10.9767/bcrec.13.3.1964.553-559 ◽

2018 ◽

Vol 13 (3) ◽

pp. 553

Author(s):

Vita Wonoputri ◽

Subiantoro Subiantoro ◽

Made Tri Ari Penia Kresnowati ◽

Ronny Purwadi

Keyword(s):

Solid State ◽

Trichoderma Reesei ◽

Solid State Fermentation ◽

Trichoderma Viride ◽

Cellulase Activity ◽

Cellulase Production ◽

Contact Method ◽

Empty Fruit Bunch ◽

Liquid Ratio ◽

Fermentation Parameters

In this study, agriculture waste palm empty fruit bunch (EFB) was used as carbon/cellulose source in solid state fermentation for cheaper cellulase production. Fermentation operation parameters, such as: solid to liquid ratio, temperature, and pH, were varied to study the effect of those parameters towards crude cellulase activity. Two different fungi organisms, Trichoderma viride and Trichoderma reesei were used as the producers. Extracellular cellulase enzyme was extracted using simple contact method using citrate buffer. Assessment of the extracted cellulase activity by filter paper assay showed that Trichoderma viride is the superior organism capable of producing higher cellulase amount compared to Trichoderma reesei at the same fermentation condition. The optimum cellulase activity of 0.79 FPU/g dry substrate was obtained when solid to liquid ratio used for the fermentation was 1:1, while the optimum fermentation temperature and pH were found to be 30 °C and 5.5, respectively. The result obtained in this research showed the potential of EFB utilization for enzyme production. Copyright © 2018 BCREC Group. All rights reservedReceived: 14th December 2017; Revised:29th July 2018; Accepted: 3rd August 2018How to Cite: Wonoputri, V., Subiantoro, S., Kresnowati, M.T.A.P., Purwadi, R. (2018). Solid State Fermentation Parameters Effect on Cellulase Production from Empty Fruit Bunch. Bulletin of Chemical Reaction Engineering & Catalysis, 13 (3): 553-559 (doi:10.9767/bcrec.13.3.1964.553-559)Permalink/DOI: https://doi.org/10.9767/bcrec.13.3.1964.553-559

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Microbial Strategies for Cellulase and Xylanase Production through Solid-State Fermentation of Digestate from Biowaste

Sustainability ◽

10.3390/su10072433 ◽

2018 ◽

Vol 10 (7) ◽

pp. 2433 ◽

Cited By ~ 14

Author(s):

Laura Mejias ◽

Alejandra Cerda ◽

Raquel Barrena ◽

Teresa Gea ◽

Antoni Sánchez

Keyword(s):

Solid State ◽

Enzymatic Activity ◽

Trichoderma Reesei ◽

Solid State Fermentation ◽

Batch Reactor ◽

Hydrolytic Enzymes ◽

Cellulase Production ◽

Degree Of Hydrolysis ◽

Xylanase Production ◽

Specific Oxygen Uptake Rate

Solid-state fermentation (SSF) is a promising technology for producing bioproducts from organic wastes. The objective of this study is to assess the feasibility of using digestate as substrate to produce hydrolytic enzymes, mainly cellulase and xylanase, by exploring three different inoculation strategies: (i) SSF with autochthonous microbiota; (ii) non-sterile SSF inoculated with Trichoderma reesei and (iii) sequential batch operation to select a specialized inoculum, testing two different residence times. Native microbial population did not show a significant cellulase production, suggesting the need for a specialized inoculum. The inoculation of Trichoderma reesei did not improve the enzymatic activity. On the other hand, inconsistent operation was achieved during sequential batch reactor in terms of specific oxygen uptake rate, temperature and enzymatic activity profile. Low cellulase and xylanase activities were attained and the main hypotheses are non-appropriate biomass selection and some degree of hydrolysis by non-targeted proteases produced during fermentation.

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