scholarly journals Serum immunoglobulins and anti-pneumococcal antibody levels in patients with bronchiectasis of unknown aetiology

2020 ◽  
Vol 19 (2) ◽  
pp. 200-207
Author(s):  
Mustafa Norhazlin ◽  
Asrul Abdul Wahab ◽  
Mohd Faisal Abdul Hamid ◽  
Hamzaini Abdul Hamid ◽  
Husyairi Harunarashid
Keyword(s):  
Streptococcus Pneumoniae ◽  
Specific Antibody ◽  
Capsular Polysaccharide ◽  
Medical Science ◽  
Asian Population ◽  
Immunoglobulin Therapy ◽  
Female Gender ◽  
Total Serum ◽  
Capsular Polysaccharides ◽  
Serum Immunoglobulins

Background: Bronchiectasis is a chronic condition which can result in significant physical and social morbidity. The exact prevalence in Malaysia is unknown although several studies have shown a higher prevalence in the Asian population. Several causative factors have been identified but there are many patients with unknown aetiologies. This study looks into the level of serum immunoglobulins and antipenumococcal antibody in bronchiectasis patients where they were not part of prior routine investigations. Methodology: Four hundred fifteen bronchiectasis patients were screened and 26 patients who fulfilled the inclusion and exclusion criteria were enrolled for this study. The serum immunoglobulins (IgG, IgA and IgM) concentrations were measured using nephelometry and interpreted according to age-matched reference range. The integrity of antibody production against specific antibody to capsular polysaccharides of Streptococcus pneumoniae were assessed using ELISA method and the level of ≥ 10mg/L is considered as reactive. Results: The twenty six bronchiectasis patients have the mean age of 62 years and a predilection of female gender. Majority of patients presented with typical bronchiectasis symptoms which were further supported by radiological findings. One of 26 patients (4%) had low total serum IgG level. The vaccinated group has higher anti-pneumococcal capsular polysaccharide antibody level (median: 224.2 mg/L) compared to the unvaccinated group (median: 100.4 mg/L). However there is no statistical difference between the anti-PCP levels of both groups (p> 0.05). All of the selected patients had reactive specific antibody to capsular polysaccharides of Streptococcus pneumoniae regardless of the vaccination status, which may reflect the natural acquisition of anti-pneumococcal immunity. Conclusion: Although immunoglobulin deficiency is an uncommon aetiological cause of bronchiectasis, the immunoglobulin parameters can be helpful in selecting patients who should receive the appropriate treatment of immunoglobulin therapy for the prevention of subsequent complications and better quality of life. Bangladesh Journal of Medical Science Vol.19(2) 2020 p.200-207

scholarly journals Production of Capsular Polysaccharide ofStreptococcus pneumoniae Type 14 and Its Purification by Affinity Chromatography

2001 ◽  
Vol 67 (2) ◽  
pp. 969-971 ◽  
Author(s):  
Norma Suárez ◽  
Laura Franco Fraguas ◽  
Esther Texeira ◽  
Hugo Massaldi ◽  
Francisco Batista-Viera ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Affinity Chromatography ◽  
Efficient Method ◽  
Capsular Polysaccharide ◽  
Capsular Polysaccharides ◽  
Affinity Adsorbent ◽  
Soybean Lectin

ABSTRACT We describe a rapid and efficient method for producing the capsular polysaccharide of Streptococcus pneumoniae by fermentation on tryptic soy broth and purification of this compound by using immobilized soybean lectin as an affinity adsorbent. In principle, the same strategy can be used to produce purified capsular polysaccharides from other streptococcal serotypes by selecting the appropriate lectin adsorbents.

scholarly journals Identification of the Smallest Structure Capable of Evoking Opsonophagocytic Antibodies against Streptococcus pneumoniae Type 14

2008 ◽  
Vol 76 (10) ◽  
pp. 4615-4623 ◽  
Author(s):  
Dodi Safari ◽  
Huberta A. T. Dekker ◽  
John A. F. Joosten ◽  
Dirk Michalik ◽  
Adriana Carvalho de Souza ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Antibody Response ◽  
Conjugate Vaccine ◽  
Specific Antibody ◽  
Capsular Polysaccharide ◽  
Specific Antibody Response ◽  
Specific Antibodies

ABSTRACT Synthetic overlapping oligosaccharide fragments of Streptococcus pneumoniae serotype 14 capsular polysaccharide (Pn14PS), {6)-[β-d-Galp-(1→4)-]β-d-GlcpNAc-(1→3)-β-d-Galp-(1→4)-β-d-Glcp-(1→}n, were conjugated to CRM197 protein and injected into mice to determine the smallest immunogenic structure. The resulting antibodies were then tested for Pn14PS specificity and for their capacity to promote the phagocytosis of S. pneumoniae type 14 bacteria. Earlier studies have reported that the oligosaccharide corresponding to one structural repeating unit of Pn14PS, i.e., Gal-Glc-(Gal-)GlcNAc, induces a specific antibody response to Pn14PS. The broader study described here, which evaluated 16 oligosaccharides, showed that the branched trisaccharide element Glc-(Gal-)GlcNAc is essential in inducing Pn14PS-specific antibodies and that the neighboring galactose unit at the nonreducing end contributes clearly to the immunogenicity of the epitope. Only the oligosaccharide conjugates that produce antibodies recognizing Pn14PS were capable of promoting the phagocytosis of S. pneumoniae type 14. In conclusion, the branched tetrasaccharide Gal-Glc-(Gal-)GlcNAc may be a serious candidate for a synthetic oligosaccharide conjugate vaccine against infections caused by S. pneumoniae type 14.

Synthesis of building blocks for an iterative approach towards oligomers of the Streptococcus pneumoniae type 1 zwitterionic capsular polysaccharide repeating unit

2016 ◽  
Vol 94 (11) ◽  
pp. 940-960 ◽  
Author(s):  
Aisling Ní Cheallaigh ◽  
Stefan Oscarson
Keyword(s):  
Streptococcus Pneumoniae ◽  
T Cell ◽  
Capsular Polysaccharide ◽  
Building Blocks ◽  
Capsular Polysaccharides ◽  
Iterative Approach ◽  
Efficient Synthesis ◽  
The Common

Zwitterionic capsular polysaccharide extracts, ∼8 kDa in mass, from Streptococcus pneumoniae type 1 (Spt1) have shown unique T-cell activating properties. Oligomers of the trisaccharide repeating unit of the Spt1 capsular polysaccharide [→3)-4-NH2-α-d-QuipNAc-(1→4)-α-d-GalpA-(1→3)-α-d-GalpA-(1-]n of defined length are needed to further investigate this response. An approach towards iteratively extendable trisaccharide building blocks of the zwitterionic capsular polysaccharides of Spt1 is described. Key elements include the comparison of pre-glycosylation oxidation and post-glycosylation oxidation approaches using thioglycoside donors to the target trisaccharide, the optimisation of the post-glycosylation oxidation approach, and the conversion of the trisaccharide to building blocks tailored for iterative glycosylation. The construction and evaluation of stereotunable 2-N-3-O-oxazolidinone donors for the common bacterial 2-acetamido-4-amino-2,4,6-trideoxy-α-d-galactopyranoside motif is also described, as is a key intermediate for their efficient synthesis.

scholarly journals Evaluation of the Specificity of Pneumococcal Polysaccharide Enzyme-Linked Immunosorbent Assay and the Effect of Serum Adsorption Based on Standard Pneumococcal Serogroup- or Serotype-Specific Rabbit Antisera

2009 ◽  
Vol 16 (9) ◽  
pp. 1279-1284 ◽  
Author(s):  
Hans-Christian Slotved ◽  
Christina Guttmann ◽  
Charlotte Demuth Pedersen ◽  
Jasper Neergaard Jacobsen ◽  
Karen Angeliki Krogfelt
Keyword(s):  
Cell Wall ◽  
Streptococcus Pneumoniae ◽  
Human Serum ◽  
Immunosorbent Assay ◽  
Capsular Polysaccharide ◽  
Enzyme Linked Immunosorbent Assay ◽  
Capsular Polysaccharides ◽  
Who Guidelines ◽  
Specific Antibodies ◽  
Specific Rabbit

ABSTRACT Worldwide, Streptococcus pneumoniae (pneumococcus) is a major cause of morbidity and mortality, especially in infants and elderly people. Pneumococcal capsular polysaccharides are well characterized, and more than 90 different serotypes have been identified. Serotype-specific antibodies against the capsular polysaccharide are produced during infection. Detection of antibodies against pneumococci by enzyme-linked immunosorbent assay (ELISA) is performed according to WHO guidelines, using antigens provided by ATCC. However, testing the ELISA for specificity is challenging due to the difficulty in obtaining human naïve serum with pneumococcal antibodies as well as human serum with antibodies against a single serotype. The application of well-defined serotype-specific sera produced in animals to evaluate the specificity of the ATCC antigens and the effect of adsorption with cell wall and 22F polysaccharides has not been performed before, to our knowledge. In this study, the specificity of ATCC antigens (serotypes 4, 6B, 9V, 14, 18C, 19F, and 23F) was tested by using commercial serotype-, serogroup-, and pool-specific pneumococcal rabbit antisera.

The structure of the specific capsular polysaccharide of Streptococcus pneumoniae type 11F (American type 11)

1985 ◽  
Vol 63 (9) ◽  
pp. 953-968 ◽  
Author(s):  
James C. Richards ◽  
Malcolm B. Perry ◽  
Peter J. Kniskern
Keyword(s):  
Streptococcus Pneumoniae ◽  
Capsular Polysaccharide ◽  
Optical Rotation ◽  
Periodate Oxidation ◽  
Capsular Polysaccharides ◽  
Glycerol Phosphate ◽  
Part D ◽  
Magnetic Resonance Studies ◽  
Specific Polysaccharide ◽  
Structure Formula

The specific polysaccharide of Streptococcus pneumoniae type 11F (American type 11) is composed of 2-acetamido-2-deoxy-D-glucose (one part), D-glucose (one part), D-galactose (two parts), ribitol (one part), phosphate (one part), and O-acetyl (two parts). Hydrolysis, dephosphorylation, periodate oxidation, methylation, optical rotation, and 1H and 13C nuclear magnetic resonance studies showed that the polysaccharide is an unbranched linear polymer of a ribitol-phosphate substituted repeating tetrasaccharide unit having the structure:[Formula: see text]The specific capsular polysaccharides of S. pneumoniae type 11B and 11C (American types 76 and 53) were found to have the same tetrasaccharide repeating unit as the 11F polysaccharide, but differed from it in their mode of O-acetylation and the replacement of the ribitol phosphate by glycerol phosphate in the 11C specific polysaccharide.

scholarly journals Acquired, but Not Innate, Immune Responses to Streptococcus pneumoniae Are Compromised by Neutralization of CD40L

2000 ◽  
Vol 68 (2) ◽  
pp. 511-517 ◽  
Author(s):  
Young-il Hwang ◽  
Moon H. Nahm ◽  
David E. Briles ◽  
David Thomas ◽  
Jeffrey M. Purkerson
Keyword(s):  
Streptococcus Pneumoniae ◽  
Capsular Polysaccharide ◽  
Pneumococcal Infection ◽  
Systemic Infection ◽  
Humoral Response ◽  
Nasal Carriage ◽  
The Elderly ◽  
Antibody Responses ◽  
Capsular Polysaccharides ◽  
Cell Wall Polysaccharides

ABSTRACT Streptococcus pneumoniae is a significant pathogen of young children and the elderly. Systemic infection by pneumococci is a complex process involving several bacterial and host factors. We have investigated the role of CD40L in host defense against pneumococcal infection. Treatment of mice with MR-1 antibody (anti-CD154/CD40L) markedly reduced antibody responses to the pneumococcal protein PspA, elicited by immunization of purified protein or whole bacteria. In mice immunized with whole bacteria, MR-1 treatment reduced antibody responses to capsular polysaccharides but not cell wall polysaccharides. MR-1 did not suppress antibody responses to isolated capsular polysaccharides but did reduce the production of antibody to a capsular polysaccharide-protein conjugate, indicating that when presented in the context of whole bacteria, the humoral response to capsular polysaccharides is partially T-cell dependent. Despite the reduction of the protective humoral responses to pneumococcal infection, administration of MR-1 had no effect on sepsis, lung infection, or nasal carriage in nonimmune mice inoculated with virulent pneumococci. Thus, short-term neutralization of CD40L does not compromise innate host defenses against pneumococcal invasion.

scholarly journals Capsular Polysaccharide Expression in CommensalStreptococcusSpecies: Genetic and Antigenic Similarities toStreptococcus pneumoniae

mBio ◽  
2016 ◽  
Vol 7 (6) ◽  
Author(s):  
Uffe B. Skov Sørensen ◽  
Kaihu Yao ◽  
Yonghong Yang ◽  
Hervé Tettelin ◽  
Mogens Kilian
Keyword(s):  
Streptococcus Pneumoniae ◽  
Virulence Factors ◽  
Capsular Polysaccharide ◽  
Capsular Polysaccharides ◽  
Structural Polymorphism ◽  
Commensal Microbiota ◽  
Capsule Production ◽  
Genetic Mechanisms ◽  
Different Origins ◽  
Host Parasite

ABSTRACTExpression of a capsular polysaccharide is considered a hallmark of most invasive species of bacteria, includingStreptococcus pneumoniae, in which the capsule is among the principal virulence factors and is the basis for successful vaccines. Consequently, it was previously assumed that capsule production distinguishesS. pneumoniaefrom closely related commensals of the mitis group streptococci. Based on antigenic and genetic analyses of 187 mitis group streptococci, including 90 recognized serotypes ofS. pneumoniae, we demonstrated capsule production by the Wzy/Wzx pathway in 74% of 66S. mitisstrains and in virtually all tested strains ofS. oralis(subspeciesoralis,dentisani, andtigurinus) andS. infantis. Additional analyses of genomes ofS. cristatus,S. parasanguinis,S. australis,S. sanguinis,S. gordonii,S. anginosus,S. intermedius, andS. constellatusrevealed complete capsular biosynthesis (cps) loci in all strains tested. Truncatedcpsloci were detected in three strains ofS. pseudopneumoniae, in 26% ofS. mitisstrains, and in a singleS. oralisstrain. The level of sequence identities ofcpslocus genes confirmed that the structural polymorphism of capsular polysaccharides inS. pneumoniaeevolved by import ofcpsfragments from commensalStreptococcusspecies, resulting in a mosaic of genes of different origins. The demonstrated antigenic identity of at least eight of the numerous capsular polysaccharide structures expressed by commensal streptococci with recognized serotypes ofS. pneumoniaeraises concerns about potential misidentifications in addition to important questions concerning the consequences for vaccination and host-parasite relationships both for the commensals and for the pathogen.IMPORTANCEExpression of a capsular polysaccharide is among the principal virulence factors ofStreptococcus pneumoniaeand is the basis for successful vaccines against infections caused by this important pathogen. Contrasting with previous assumptions, this study showed that expression of capsular polysaccharides by the same genetic mechanisms is a general property of closely related species of streptococci that form a significant part of our commensal microbiota. The demonstrated antigenic identity of many capsular polysaccharides expressed by commensal streptococci andS. pneumoniaeraises important questions concerning the consequences for vaccination and host-parasite relationships both for the commensals and the pathogen.

scholarly journals Synthesis and Preliminary Immunological Evaluation of a Pseudotetrasaccharide Related to a Repeating Unit of the Streptococcus pneumoniae Serotype 6A Capsular Polysaccharide

2021 ◽  
Vol 8 ◽  
Author(s):  
Elena V. Sukhova ◽  
Dmitry V. Yashunsky ◽  
Ekaterina A. Kurbatova ◽  
Elina A. Akhmatova ◽  
Yury E. Tsvetkov ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Capsular Polysaccharide ◽  
Capsular Polysaccharides ◽  
Immunological Evaluation

2-Aminoethyl glycoside of the pseudotetrasaccharide α-d-Glcp-(1→3)-α-l-Rhap-(1→3)-d-Rib-ol-(5-P-2)-α-d-Galp corresponding to a repeating unit of the Streptococcus pneumoniae type 6A capsular polysaccharide has been synthesized. A suitably protected pseudotrisaccharide α-d-Glcp-(1→3)-α-l-Rhap-(1→3)-d-Rib-ol with a free 5-OH group in the ribitol moiety and a 2-OH derivative of 2-trifluoroacetamidoethyl α-d-galactopyranoside have been efficiently prepared and then connected via a phosphate bridge using the hydrogen phosphonate procedure. Preliminary immunological evaluation of this pseudotetrasaccharide and the previously synthesized pseudotetrasaccharide corresponding to a repeating unit of the capsular polysaccharide of S. pneumoniae serotype 6B has shown that they contain epitopes specifically recognized by anti-serogroup 6 antibodies and are able to model well the corresponding capsular polysaccharides. Conjugates of the synthetic pseudotetrasaccharides with bovine serum albumin were shown to be immunogenic in mice.

scholarly journals Selectively Charged and Zwitterionic Analogues of the Smallest Immunogenic Structure of Streptococcus Pneumoniae Type 14

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3414 ◽  
Author(s):  
Tiziana Gragnani ◽  
Doretta Cuffaro ◽  
Silvia Fallarini ◽  
Grazia Lombardi ◽  
Felicia D’Andrea ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Specific Antibody ◽  
Capsular Polysaccharide ◽  
Negative Charge ◽  
Positive Charge ◽  
Research Community ◽  
Competitive Elisa ◽  
Structural Variations ◽  
Immunological Properties ◽  
Neutral Compound

Zwitterionic polysaccharides (ZPs) have been shown in recent years to display peculiar immunological properties, thus attracting the interest of the carbohydrate research community. To fully elucidate the mechanisms underlying these properties and exploit the potential of this kind of structures, in depth studies are still required. In this context, the preparation of two cationic, an anionic, as well as two zwitterionic tetrasaccharide analogues of the smallest immunogenic structure of Streptococcus pneumoniae type 14 (SP14) capsular polysaccharide are presented. By exploiting a block strategy, the negative charge has been installed on the non-reducing end of the lactose unit of the tetrasaccharide and the positive charge either on the non-reducing end of the lactosamine moiety or on an external linker. These structures have then been tested by competitive ELISA, showing that the structural variations we made do not modify the affinity of the neutral compound to binding to a specific antibody. However, lower efficacies than the natural SP14 compound were observed. The results obtained, although promising, point to the need to further elongate the polysaccharide structure, which is likely too short to cover the entire epitopes.

Sign in / Sign up

Export Citation Format

Share Document