Estimation of vitamin B12 in plasma by High Performance Liquid Chromatography

<p>High performance liquid chromatography was used to develop and validate the detection of vitamin B₁₂ in blood plasma sample. The mobile phase consists of a mixture of 0.025% trifluroacetic acid in deionized water and 30% methanol. The mixture used was adjusted to pH 2.9 and the flow rate was adjusted to 0.5 mL/min. The separation was achieved using C18 column maintained at 30ºC temperature and detection of vitamin B₁₂ was conducted at maximum wavelength 230 nm.</p><p><strong>Video Clip of Methodology</strong>:</p><p>Estimation of vitamin B12 in plasma by HPLC: 4 min 29 sec <a href="https://www.youtube.com/v/0eMxqsdDtlE">Full Screen</a> <a href="https://www.youtube.com/watch?v=0eMxqsdDtlE">Alternate</a></p>