The formation of fibrin clots determines the characteristics of blood plasma coagulation. It is known that small molecules like nitrogen and carbon monoxides have a toxic effect at high concentrations due to the competitive binding to hemoglobin, preventing further oxygen transfer. The mechanisms of intoxication with these gases have been extensively studied in the literature, but there is little information on their effects on other vital processes. In particular, blood coagulation parameters have not been studied, although prolonged exposure to relatively low concentrations of gases can cause significant pathological changes. In this paper, the characteristics of the fibrin-polymer after coagulation of a blood plasma sample under conditions of pretreatment with gases were studied using FTIR-spectroscopy. The changes in the vibrations of individual bonds and fragments in the Amide I and Amide B regions are shown and analyzed. It was established that at concentrations of CO exceeding the endogenous levels, the connector α-helix unfolds and β-structures form, leading to the loss of part of the hydrate shell, the formation of a fibrin clot with a disordered structure of higher density due to an increase in its hydrophobicity. For cases where samples were treated with NO gas, the degree of aggregation of the fibrin clot from plasma incubated with nitroglycerin was one-quarter less than the original, the proportion of α-helices was not reduced, and there were no disordered structures in the clot. This may indicate a lower clot density and probably easier lysis of the one.
Comparison of blood plasma sample preparation methods for combined LC–MS lipidomics and metabolomics
