scholarly journals ISSR markers and population differentiations in Erodium ciconium (L.) L'hér ex Aiton

2021 ◽  
Vol 28 (1) ◽  
pp. 61-73
Author(s):  
Xi Fei ◽  
Ruan Xuejun ◽  
Amir Abbas Minaeifar
Keyword(s):  
Population Genetic ◽  
Genetic Difference ◽  
Morphological Variability ◽  
Molecular Data ◽  
Issr Markers ◽  
Mantel Test ◽  
Population Diversity ◽  
Protein Supplements ◽  
Population Genetic Differentiation ◽  
Plant Taxon

Erodium ciconium is an important grazing plant and a source of protein supplements to straw for ruminants in semideserts and wastelands of the Middle East. There is no information on its population genetic structure, genetic diversity, and morphological variability in Iran. We performed molecular data for knowing the population differentiation in this species. For this study, we used 110 randomly collected plants from 15 geographical populations in 6 provinces of Iran. AMOVA test revealed significant genetic difference among the studied populations and also revealed that, 63% of total genetic variability was due to within population diversity while, 37% was due to among population genetic differentiation. Mantel test showed positive significant correlation between genetic distance and geographical distance of the studied populations. Networking, STRUCTURE analyses revealed some degree of gene flow among these populations. Bangladesh J. Plant Taxon. 28(1): 61-73, 2021 (June)

scholarly journals Population genetic structure and gene flow in Alcea aucheri (Boiss.) Alef.: A potential medicinal plant

Genetika ◽  
2021 ◽  
Vol 53 (2) ◽  
pp. 867-882
Author(s):  
Liu Hang ◽  
Li Pan ◽  
Tang Yong ◽  
Luo Jianguo ◽  
Xu Xingmin ◽  
...  
Keyword(s):  
Gene Flow ◽  
Genetic Structure ◽  
Population Genetic Structure ◽  
Population Genetic ◽  
Genetic Difference ◽  
Morphological Variability ◽  
Molecular Data ◽  
Mantel Test ◽  
Population Diversity ◽  
Population Genetic Differentiation

The genus Alcea, a member of Malvaceae family consists of approximately 75 species worldwide distributing mainly in South-West Asia. Among these, 33 species grow in Iran. Plants of the Alcea (or Althaea) are among important medicinal plants in Iranian traditional medicine. They have long been used in the treatment of health problems and diseases. Alcea aucheri (Boiss.) Alef. species are distributed in different habitats of Iran. There is no information on its population genetic structure, genetic diversity, and morphological variability in Iran. Therefore, due to the importance of these plant species, we performed a combination of morphological and molecular data for this species. For this study, we used 118 randomly collected plants from 10 geographical populations in 5 provinces. AMOVA test revealed significant genetic difference among the studied populations and also revealed that, 74% of total genetic variability was due to within population diversity while, 26% was due to among population genetic differentiation. Mantel test showed positive significant correlation between genetic distance and geographical distance of the studied populations. Networking, STRUCTURE analyses revealed some degree of gene flow among these populations.

Species delimitation and population structure in three Onosma (Boraginaceae) species

Biologia ◽  
2015 ◽  
Vol 70 (10) ◽  
Author(s):  
Masoud Sheidai ◽  
Samira Sadeghi ◽  
Mahnaz-Arab Ameri ◽  
Ahmad-Reza Mehrabeian
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Species Delimitation ◽  
Genetic Difference ◽  
Morphological Variability ◽  
Morphological Diversity ◽  
Mantel Test ◽  
Geographical Distance ◽  
Nj Tree ◽  
Issr Molecular Markers

AbstractThe present study aimed to reveal genetic diversity and population structure in three Onosma species i.e., Onosma bulbotrichum DC., O. elwendicum Wettst. and O. sericeum Willd. (Boraginaceae) growing in Iran. This study was performed to check if the ISSR molecular markers could be used in the Onosma species delimitation. We also investigated the infra-specific morphological variability and if we can identify infra-species taxonomic forms. The present study revealed within species genetic and morphological diversity. ISSR data could delimit the studied species as they were separated from each other in NJ tree, maximum parsimony and STRUCTURE analysis. AMOVA and Hickory tests produced significant genetic difference among the studied species. The Mantel test showed no correlation between genetic distance and geographical distance of the populations for all three species. Reticulation analysis and Nm estimation showed gene flow among these populations. We could identify a few ISSR loci that are adaptive. Data obtained can be used in conservation of these medicinal plants in Iran.

scholarly journals Understanding population differentiation using geographical, morphological and genetic characterization in Erodium cicunium

2020 ◽  
Vol 80 (04) ◽  
Author(s):  
Xingxing Si ◽  
Lei GAO ◽  
Yuwei Song ◽  
Majid Khayatnezhad ◽  
Amir Abbas Minaeifar
Keyword(s):  
Genetic Difference ◽  
Morphological Characters ◽  
Molecular Data ◽  
Population Diversity ◽  
Start Codon ◽  
Principal Coordinates Analysis ◽  
Forage Plants ◽  
Principal Coordinates ◽  
Geographical Populations ◽  
Study Population

Erodium cicunium (Geraniaceae) species are distributed in different habitats of Iran. Some species are of medicinal importance while some are well known weeds and used as forage plants. An investigation was carried out to evaluate 124 randomly collected plants of E. cicunium from 15 geographical populations in 5 provinces to study population structure and for morphological and molecular characters. Start Codon Targeted (SCoT) markers were used to analyse molecular diversity. Analysis of molecular variance (AMOVA) revealed significant genetic difference among the studied populations and also revealed that 60% of total genetic variability was due to within population diversity, while 40% genetic differentiation was recorded among populations. Principal Coordinates Analysis (PCoA) of populations based on morphological characters was not in agreement with Metric Multidimensional Scaling (MDS) plot of molecular data.

scholarly journals Species relationship and genetic diversity in some Iranian Lamium L. species using ISSR markers

2019 ◽  
Vol 20 (7) ◽  
Author(s):  
FATEMEH AZIMISHAD ◽  
MASOUD SHEIDAI ◽  
SAYED MEHDI TALEBI ◽  
ZAHRA NOORMOHAMMADI
Keyword(s):  
Genetic Diversity ◽  
High Efficiency ◽  
Isolation By Distance ◽  
Leaf Shape ◽  
Morphological Characteristics ◽  
Molecular Data ◽  
Issr Markers ◽  
Mantel Test ◽  
Species Relationships ◽  
Species Relationship

Abstract. Azimishad F, Sheidai M, Talebi SM, Noormohammadi Z. 2019. Species relationship and genetic diversity in some Iranian Lamium L. species using ISSR markers. Biodiversitas 20: 1963-1972. Lamium is a widespread and taxonomically complex genus of Lamiaceae which comprises of 16-38 species. This genus is represented in Iran by nine species. In the present study, we used morphological and molecular (ISSR, Cp DNA, and nrITS) data to evaluate species relationships, genetic diversity and population genetic structure of the genus. 27 morphological characteristics, including 13 qualitative and 14 quantitative, and ten ISSR markers were used for morphological and genetical evaluation of 73 accessions from eight taxa. In general, species relationships obtained from morphological and molecular data were largely congruent. In the morphological study, characteristics like the life form, leaf shape, absence/existence of bracts and shape of corolla, were distinctive traits and we did not encounter intermediate forms. Our findings indicated a very high efficiency of the ISSR markers in the identification and delimitation of Lamium species. These results confirmed the placement of L. galeobdolon in the genus Lamium and segregation of L. purpureum and L. garganicum in section Lamium. AMOVA analysis revealed that the species of this genus are genetically differentiated. Nm analysis showed very low value of gene flow among the studied species and mantel test indicated isolation by distance occurred among them.

scholarly journals Population differentiation and gene flow in Rrodium cicutarium: A potential medicinal plant

Genetika ◽  
2020 ◽  
Vol 52 (3) ◽  
pp. 1127-1144 ◽  
Author(s):  
Yaocheng Jia ◽  
Majid Khayatnezhad ◽  
Shahram Mehri
Keyword(s):  
Genetic Diversity ◽  
Gene Flow ◽  
Genetic Variability ◽  
Population Genetic ◽  
Morphological Variability ◽  
Morphological Characteristics ◽  
Assignment Test ◽  
Population Genetic Differentiation ◽  
Irap Markers ◽  
Geographical Populations

Erodium (Geranaiceae) species are distributed in different habitats of Iran. Some species are of medicinal importance while some are well known weeds. In arid and semi-arid regions, E. cicutarium has had some importance as a forage plant and is an important grazing plant and source of protein supplements to straw for ruminants in semi deserts and wastelands of the Middle East. There is no information on its population genetic structure, genetic diversity, and morphological variability in Iran. Due to the medicinal importance of this species, a genetic variability and populations? structure study is performed studying 15 geographical populations of E. cicutarium Therefore, we used six inter-retrotransposon amplified polymorphism (IRAP) markers and 15 combined IRAP markers to reveal within and among population genetic diversity in this plant. AMOVA test produced significant genetic difference (PhiPT = 0.39, P = 0.010) among the studied populations and also revealed that, 55% of total genetic variability was due to within population diversity while, 45% was due to among population genetic differentiation. Mantel test showed positive significant correlation between genetic distance and geographical distance of the studied populations. Networking, STRUCTURE analyses and population assignment test revealed some degree of gene flow among these populations. PCoA plot of populations based on morphological characters was in agreement with MDS plot of molecular data. These results indicated that geographical populations of E. cicutarium are well differentiated both in genetic content as well as morphological characteristics. Consensus tree based on morphological and genetic data separated some of these populations from the others suggesting the existence of ecotypes within this species.

scholarly journals Genetic and morphological diversity in Cousinia calocephala Jaub. & Spach (Asteraceae; section Cynaroideae bunge) populations

Genetika ◽  
2020 ◽  
Vol 52 (1) ◽  
pp. 199-213
Author(s):  
Neda Atazadeh ◽  
Masoud Sheidai ◽  
Farideh Attar ◽  
Fahimeh Koohdar
Keyword(s):  
Genetic Difference ◽  
Morphological Difference ◽  
Morphological Diversity ◽  
Issr Markers ◽  
Mantel Test ◽  
Zagros Mountains ◽  
Discriminating Power ◽  
Geographical Populations ◽  
And Migration ◽  
Almost All

The genus Cousinia Cass. of the tribe Cardueae with about 700 species is one of the most diverse genera in Central and SW Asia following Senecio and Vernonia. The section Cynaroideae with 89 species is the largest section of the genus. Cousinia calocephala is the only endemic species of the section distributed in 14 provinces of Iran from Alborz to Zagros mountains. In present study 65 plant specimens of 13 geographical populations of C. calocephala were investigated based on the morphological and genetic (ISSR) data. ANOVA test revealed a significant morphological difference among the studied populations. Similarly, AMOVA test yielded a significant genetic difference between the studied populations, suggesting that the studied populations are morphologically and genetically differentiated. AMOVA test revealed that 94% of the total genetic difference was due to inter-populations genetic differences, while 6% was due to within-species genetic variability. The discriminating power of ISSR loci as determined by Gst against Nm analysis, revealed that almost all ISSR loci have an excellent discriminating power. Thus, ISSR markers are efficient in differentiating of the studied C. calocephala populations. The mantel test, revealed a significant positive correlation between genetic and morphological distance and geographical distance of the studied populations. Genetic analysis results revealed that along with genetic drift, low level of gene flow and migration, adaptive loci also helped populations diverge and adapt these populations to their local condition. Thus, we have three different groups which can be considered as three ecotypes for C. calocephala based on the morphological and genetic data.

scholarly journals Genetic diversity and morphological variability in Geranium purpureum Vill. (Geraniaceae) of Iran

Genetika ◽  
2017 ◽  
Vol 49 (2) ◽  
pp. 543-557 ◽  
Author(s):  
Somayeh Bozchaloyi ◽  
Masoud Sheidai ◽  
Maryam Keshavarzi ◽  
Zahra Noormohammadi
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Morphological Variability ◽  
Morphological Characteristics ◽  
Morphological Characters ◽  
Molecular Data ◽  
Mantel Test ◽  
Geographical Populations ◽  
Well Differentiated ◽  
Genetic Stratification

Genetic variability and populations, structure were studied in 15 geographical populations of Geranium purpureum Vill. (Geraniaceae). Genetic diversity parameters were determined in these populations. AMOVA and Gst analyses revealed the presence of genetic variability within populations and significant molecular difference among the studied populations. Mantel test showed positive significant correlation between genetic distance and geographical distance of the studied populations. STRUCTURE and K-Means clustering revealed populations, genetic stratification. UPGMA dendrogram of populations based on morphological characters was in agreement with NJ tree of molecular data. These results indicated that geographical populations of G. purpureum are well differentiated both in genetic content as well as morphological characteristics. Consensus tree based on morphological and genetic data separated some of these populations from the others suggesting the existence of ecotypes within this species.

Genetic and morphological diversity in Stachys lavandulifolia (Lamiaceae) populations

Biologija ◽  
2016 ◽  
Vol 62 (1) ◽  
Author(s):  
Masoud Sheidai ◽  
Fatemeh Taban ◽  
Seyed Mehdi Talebi ◽  
Zahra Noormohammadi
Keyword(s):  
Genetic Diversity ◽  
Medicinal Plant ◽  
Gene Diversity ◽  
Genetic Difference ◽  
Morphological Variability ◽  
Population Diversity ◽  
Morphological Data ◽  
Gene Pools ◽  
Germplasm Management ◽  
Geographical Populations

Stachys lavandulifolia Vahl. (Lamiaceae) is an important medicinal plant that grows in different parts of Iran and forms many geographical populations. We have no information on its population genetic structure, genetic diversity, and morphological variability in Iran. Therefore, we planned a genetic and morphological investigation in St. lavandulifolia geographical populations in Iran. The obtained data are important for conservation and germplasm management of this medicinal plant species. Seventy-four plants were randomly collected from 14 geographical populations and studied for genetic diversity (ISSR molecular markers) and morphological variability. The  highest value for gene diversity occurred in populations 1 and 4 (0.133 and 0.129, respectively). The  latitude and altitude were positively correlated with gene diversity and genetic polymorphism while longitude was negatively correlated with them. The Mantel test showed correlation between genetic distance and geographical distance. AMOVA revealed a significant genetic difference among populations and showed that 58% of total genetic variation was due to within-population diversity. The STRUCTURE analysis and K-Means clustering identified two gene pools for St. lavandulifolia. The  consensus tree of both molecular and morphological data identified divergent populations.

scholarly journals Genetic diversity and population structure of Iranian pistachio (Pistacia vera l.) cultivars

Genetika ◽  
2021 ◽  
Vol 53 (2) ◽  
pp. 671-686
Author(s):  
Mojdeh Mahdavi ◽  
Fariba Sharifnia ◽  
Fahimeh Salimpour ◽  
Akbar Esmaeili ◽  
Mohaddeseh Larypoor
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Genetic Difference ◽  
Genetic Relationships ◽  
Sustainable Use ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Mantel Test ◽  
Similarity Coefficients ◽  
Conservation Core

Iran has a rich pistachio germplasm, thereby, the diversity and number of Iranian pistachio cultivars is unique in the world. Genetic diversity is crucial for sustainable use of genetic resources and conservation. As one of the oldest nut crops in human history, pistachio nuts have a high nutritional value and are commercially important. In the present study, the genetic variation of pistachio genotypes was investigated by nuclear ISSR markers. In this study, genetic relationships among 11 cultivars was assessed by using 12 inter simple sequence repeat (ISSR) primers. The total of 53 bands of which 44 (83%) were polymorphic were amplified by the 12 primers, an average of 4.4 bands per primer. The total number of amplified fragments was between 2 to 6 and the number of polymorphic fragments ranged from two to six. The amplified allele sizes ranged from 300 to 1600 bp. Pair-wise genetic similarity coefficients varied from 0.70 to 0.95. The UPGMA dendrogram differentiated the genotypes into two major clusters. The Mantel test showed correlation between genetic and geographical distance. AMOVA revealed a significant genetic difference among cultivars and showed that 35% of total genetic variation was due to within- cultivars diversity. The present results may be used for the conservation, core collection and future breeding of the pistachio.

Genetic diversity and morphological variability in Polygonum aviculare s.l. (Polygonaceae) of Iran

Phytotaxa ◽  
2015 ◽  
Vol 233 (2) ◽  
pp. 166 ◽  
Author(s):  
Samaneh Mosaferi ◽  
Masoud Sheidai ◽  
Maryam Keshavarzi ◽  
Zahra Noormohammdai
Keyword(s):  
Morphological Variability ◽  
Genetic Data ◽  
Mantel Test ◽  
Gene Exchange ◽  
Consensus Tree ◽  
Polygonum Aviculare ◽  
Molecular Investigation ◽  
Variable Species ◽  
Issr Molecular Markers ◽  
Positive Significant Correlation

A molecular investigation was carried out on the variable species Polygonum aviculare using 109 plants from 12 Iranian populations. ISSR molecular markers were used for genetic study. AMOVA and Gst analyses revealed a high genetic variability both within and among populations. Mantel test showed positive significant correlation between genetic and geographical distance of populations. Structure and K-Means clustering revealed stratification ingenetic characteristics of accessions. Networking and reticulation analyses showed some degree of gene exchange among populations. Consensus tree based on morphological and genetic data separated some of these populations from the others suggesting the existence of new taxa within this species.

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