Specialized sports nutrition products using protein hydrolysis compositions of collagen-containing fish raw materials

The development of specialized sports nutrition with components of collagen-containing fish raw materials is due to the shortage of domestic products of this group, the problem of fish waste processing, scientific data on bioactive peptides of marine origin. Low-molecular active peptides and protein-mineral compositions in the form of powder and liquid dietary supplements were obtained from the scales of sardine and sardinella by methods of deep hydrolysis. During the hydrolysis of raw materials, fermentolysis was carried out with the Alcalase 2.5L enzyme, thermohydrolysis - at temperatures of 130-140 °C. The chemical composition of the scales of two fish species, the products of its hydrolysis, and the amino acid composition of its proteins have been studied. The amount of low-molecular-weight peptides in hydrolysates of sardine scales was investigated by various methods of hydrolysis. Rational methods of hydrolysis of scales - enzymatic and enzymatic-thermal - have been substantiated. They make it possible to obtain protein supplements with protein content of 83.9-85.2 % with a proportion of active peptides with a molecular weight of less than 10 kDa 91.7-98.1 %. A technology for obtaining protein supplements from fish scales has been developed, and their amino acid composition has been studied. The presence of irreplaceable and ergogenic amino acids, accompanying nitrogenous compounds, valuable in sports nutrition, was shown in the peptide supplement. An increased content of calcium, phosphorus and magnesium in the protein-mineral dietary supplement being important for the musculoskeletal tissues of an athlete has been established. On the basis of protein supplements in dry and liquid forms with the addition of beekeeping products, the technology of chewing marmalade for sports nutrition has been developed. Bioproduct "Apikolltonus" belongs to the class of gainers with a protein content of 20.5 %, a carbohydrate of 41.6 % with an amino acid value of BCAA (isoleucine : leucine : valine) 2 : 1 : 1.5. A multicomponent bar for sports nutrition has been developed using protein hydrolysates of scales, flaxseed and apple cakes, and pine nuts. The bar is functional in terms of the content of calcium and phosphorus, dietary fiber, flavonoids, and vitamin E. Recommendations for the use of new sports nutrition products are substantiated.

Protein Source and Intake Effects on Diet Digestibility and N Excretion in Horses—A Risk of Environmental N Load of Horses

Six Finnhorse mares were used in a digestibility trial, in which six typical horse diets were compared. The diets were: (A) haylage 100%; (B) hay 100%; (C) hay 70% and oats 30%; (D) hay 70% and soybean meal + oats 30%; (E) hay 70%, rapeseed meal + oats 30% and (F) hay 70 %, linseed meal + oats 30%. The trial was conducted according to an unbalanced 6 × 4 Latin square design with four 3-week experimental periods. The experimental period consisted of 17-day preliminary feeding which was followed by a 4-day total faecal and urine collection periods to evaluate N excretion. The digestibilities of DM (p < 0.001) and OM (p < 0.001) in the haylage-only diet were lower compared to the other diets. The supplemental protein feeds improved the diet digestibility of CP (p = 0.002) compared to a hay + oats diet. Furthermore, the DM (p = 0.019), OM (p = 0.006), and CP (p = 0.016) digestibilities of the soya-supplemented diet were better than those of the rapeseed- and linseed-supplemented diets. Faecal excretion was greater for haylage (19.3 kg fresh faeces and 3.6 kg DM/day) and hay (18.7 kg fresh faeces and 3.6 kg DM/day) diets (p < 0.001) compared with the other diets. Urine excretion was also greater for forage-only diets compared with diets including protein supplements. Horses excreted 14.0 L urine/day on haylage-only diet (p = 0.026) and 14.3 L/day on a hay-only diet (p = 0.003). Horses excreted more nitrogen in their urine than in dung. N excretion differed between the diets. Horses on a haylage-only diet excreted 51.6 g N in faeces /day and on hay-only diet 51.4 g N/day. On the other hand, when protein content in forages increased, N excretion via urine increased (haylage vs. dried hay). Horses excreted less N in urine with hay-only diet than with haylage-only or protein-supplemented diets (p < 0.001). When N excreted in faeces and urine was counted together, horses excreted less N with a hay-only diet (p < 0.001) than with a supplemented one (oats and/or protein feeds). The results showed that feed choices affected the amount of nitrogen excreted. Feeding recommendations should consider not only the horse category and work level, but also the protein source. When good quality protein is fed, smaller N intakes can be applied to reduce the N excretion via urine and dung. At the farm level, improved understanding of feed quality, as well as feeding planning and practices, is a way to decrease the risk of N leaching and evaporation.

Microalgae as Sources of High-Quality Protein for Human Food and Protein Supplements

As a result of population growth, an emerging middle-class, and a more health-conscious society concerned with overconsumption of fats and carbohydrates, dietary protein intake is on the rise. To address this rapid change in the food market, and the subsequent high demand for protein products, agriculture, aquaculture, and the food industry have been working actively in recent years to increase protein product output from both production and processing aspects. Dietary proteins derived from animal sources are of the highest quality, containing well-balanced profiles of essential amino acids that generally exceed those of other food sources. However, as a result of studies highlighting low production efficiency (e.g., feed to food conversion) and significant environmental impacts, together with the negative health impacts associated with the dietary intake of some animal products, especially red meats, the consumption of animal proteins has been remaining steady or even declining over the past few decades. To fill this gap, researchers and product development specialists at all levels have been working closely to discover new sources of protein, such as plant-based ingredients. In this regard, microalgae have been recognized as strategic crops, which, due to their vast biological diversity, have distinctive phenotypic traits and interactions with the environment in the production of biomass and protein, offering possibilities of production of large quantities of microalgal protein through manipulating growing systems and conditions and bioengineering technologies. Despite this, microalgae remain underexploited crops and research into their nutritional values and health benefits is in its infancy. In fact, only a small handful of microalgal species are being produced at a commercial scale for use as human food or protein supplements. This review is intended to provide an overview on microalgal protein content, its impact by environmental factors, its protein quality, and its associated evaluation methods. We also attempt to present the current challenges and future research directions, with a hope to enhance the research, product development, and commercialization, and ultimately meet the rapidly increasing market demand for high-quality protein products.

Prevalence of muscle dysmorphia symptomatology and related factors in male university students / Prevalencia de sintomatología de dismorfia muscular y factores asociados en estudiantes universitarios varones

Muscle dysmorphia is an obsessive-compulsive disorder subcategorized as a body dysmorphic disorder. Studies in Latin America have assessed the prevalence of muscle dysmorphia in bodybuilders and gym users. Therefore, the purpose of this study was to evaluate the prevalence of muscle dysmorphia symptomatology (MDS) and associated factors in university male students in Lima, Peru. The participants (N = 618) had an average age of 21.0 years (DS = 2.3). We assessed MDS through the Muscular Appearance Satisfaction Scale (MASS) and obsessive-compulsive disorders through the Yale-Brown Scale Modified for Body Dysmorphic Disorders (BDD-YBOCS). We also tested participants on their food consumption and physical activity (PA) frequency. The prevalence of MDS was 1.3% (IC 95% = 0.4-2.2%) . associated with factors such as intense PA defined as more than five hours per week (PRa = 9.5; 95% CI = 1.1-84.4) and a hyperproteic diet (PRa = 1.3; 95% CI = 1.1-1.6). Thirty-two percent of participants consumed protein supplements. Muscle dysmorphia is a disorder present in the general population. However, more research must be done to understand how this is an emerging problem in Latin America. Therefore, to influence its prevention and early identification among the university population, it is necessary to deepen the understanding.ResumenLa dismorfia muscular es un trastorno obsesivo-compulsivo subcategorizado recientemente como un trastorno dismórfico corporal. En Latinoamérica se ha estimado la prevalencia de sintomatología de dismorfia muscular (SDM), pero principalmente en usuarios de gimnasio o fisicoculturistas. Por tanto, el propósito de este estudio fue evaluar la prevalencia de SDM y factores asociados entre estudiantes universitarios de Lima, Perú. Participaron 618 varones con edad promedio de 21.0 años (DE = 2.3), quienes completaron la Escala de Satisfacción con la Apariencia Muscular (MASS) y la Escala Obsesiva-Compulsiva de Yale-Brown Modificada para Trastorno Dismórfico Corporal (BDD-YBOCS), y además fueron evaluados en cuanto a su consumo de alimentos y realización de actividad física. La prevalencia de SDM fue 1.3% (IC 95% = 0.4-2.2%), asociada a la realización de actividad física intensa (PRa = 9.5; IC 95% = 1.1-84.4) y al seguimiento de una dieta hiperproteica (PRa = 1.3; IC 95% = 1.1-1.6). Adicionalmente, 32% de los participantes indicó consumir suplementos proteicos. La DM es un trastorno emergente en Latinoamérica, por lo que es aún incipiente el conocimiento que se tiene sobre él. Por tanto, para poder incidir en su prevención e identificación temprana entre población universitaria, es necesario profundizar en su comprensión.

Measuring Prescription Pattern of Nutraceuticals with Special Focus on Protein Supplements in Tier-1 Indian City

Nutraceuticals are playing vital role in today’s time given the recent changes in lifestyle, dietary patterns, and increased stress due to work related pressure. They can be organized in several ways depending upon its ease of understanding and application, i.e. for academic instruction, clinical trial design, functional food development or dietary recommendations. A study was carried out for a period of two months to assess the awareness and prescription pattern of nutraceuticals with reference to protein supplements and to evaluate factors that will drive the future demand. Responses were obtained from Doctors and chemists from a single metro city in India. The analysis was performed using descriptive statistics and frequency distribution valid percentage was calculated. Chi-Square test was performed to measure the association between the demographic factors to preferences for protein supplements. p- Value was calculated and α- value of 0.05 or less was considered significant. The results show that the maximum protein supplement was prescribed in the age group of 20-40 years. Doctors usually prescribe protein (44%) for convalescent followed by pre and post-operative cases (40%). Haematinics are the drugs, which are co-prescribed 30% of the times with protein. Responses from Chemists showed that self-prescription was prevalent amongst their buyer and many factors were identified which leads to self-prescription, amongst which buyer’s own previous experience was found to be significant (p-value-0.03). The study has helped to reveal the prescription pattern and practise of the doctors and of the factors leading to self-prescription.

PSXVI-4 Supplemental effects of increasing levels of soy protein concentrate replacing animal protein supplements on growth performance and intestinal health of nursery pigs

This study evaluated supplemental effects of increasing levels of soy protein concentrate (SPC) replacing animal protein supplements on growth performance and intestinal health of nursery pigs. Thirty-two newly weaned pigs (6.4 ± 0.4 kg BW) were allotted to 4 treatments in a RCBD with initial BW and sex as blocks and fed for 35 d in 3 phases (P1/2/3 for 10/12/13 d, respectively) following NRC (2012). Dietary treatments were SPC-0 (diet with fish meal 4/2/1%, poultry meal 10/8/4%, and blood plasma 4/2/1% for P1/2/3, respectively); SPC-1, SPC-2, and SPC-3 (SPC-0 with SPC replacing 1/3, 2/3, and 3/3 of animal protein supplements). Titanium dioxide (0.4%) was added to the diets as indigestible marker from d 30. Growth performance was recorded for each phase. Pigs were euthanized on d 35 to collect jejunal tissue to measure intestinal health, and ileal digesta to measure apparent ileal digestibility (AID) of nutrients. Data were analyzed using the MIXED procedure of SAS. Overall, increasing levels of SPC replacing animal proteins reduced linearly (P &lt; 0.05) the BW (21.4 to 17.3 kg), ADG (431 to 312 g/d), and ADFI (551 to 390 g/d) of pigs. The G:F decreased linearly (P &lt; 0.05) on P1 (0.72 to 0.52). Increasing levels of SPC replacing animal proteins did not affect the AID of nutrients. Immunoglobulin A tended to increase (P = 0.099, 3.39 to 5.55 mg/mg of protein) when SPC supplemental levels were compared with the control group. Increasing levels of SPC replacing animal proteins did not affect the concentration of MDA, TNF-a, IL-8, protein carbonyl, and IgG and villus height, villus width, and crypt cells proliferation among treatments. Collectively, increasing level of SPC supplement did not negatively affect the intestinal health and digestibility of nutrients, whereas linearly reduced the growth performance.

PSXVI-5 Supplemental effects of soy protein concentrate replacing animal protein supplements on growth performance and intestinal health of nursery pigs

This study evaluated supplemental effects of soy protein concentrate (SPC) replacing animal protein supplements on growth performance and intestinal health of nursery pigs. Forty-eight newly weaned pigs (6.4 ± 0.6 kg BW) were allotted to 4 treatments in a RCBD with initial BW and sex as blocks fed for 35 d in 3 phases (P1/2/3 for 10/12/13 d, respectively). Dietary treatments were CON: diet with fish meal 4/2/1%, poultry meal 10/8/4%, and blood plasma 4/2/1% for P1/2/3, respectively; RFM: CON with SPC replacing fish meal; RPM: CON with SPC replacing poultry meal; and RBP: CON with SPC replacing blood plasma. Titanium dioxide (0.4%) was added to diets as indigestible marker from d 30. Growth performance was recorded for each phase. Pigs were euthanized on d 35 to collect jejunal tissue to measure intestinal health and mucosa-associated microbiota, and ileal digesta to measure apparent ileal digestibility (AID) of nutrients. Data were analyzed using the MIXED procedure of SAS. In P1, RBP decreased (P &lt; 0.05) ADFI compared to CON (202 to 152 g/d) whereas no differences on growth performance and AID (DM, CP, and AA) were observed among treatments during the overall period. The RFM tended to increase (P = 0.087) IgA (3.48 to 5.54 mg/mg of protein) without differences in MDA, TNF-a, IL-8, protein carbonyl, and IgG among treatments. The RPM increased (P &lt; 0.05) Firmicutes (14.3 to 26.1%) and Bacteroidetes (8.3 to 18.7%) but decreased (P &lt; 0.05) Proteobacteria (64.0 to 42.1%). There were no differences in villus height, villus width, and crypt cells proliferation among treatments. In conclusion, SPC could replace animal protein supplements in nursery diets without affecting growth performance and intestinal health, and AID of nutrients. Particularly SPC replacing poultry meal enhanced the balance of jejunal mucosa-associated microbiota by reducing Proteobacteria and increasing Bacteroidetes and Firmicutes.