scholarly journals Aflatoxins in bird feed collected from various areas of Lahore city

2021 ◽  
Vol 56 (4) ◽  
pp. 249-254
Author(s):  
Alim un Nisa ◽  
SH Abidi ◽  
Qurat ul Ain Syed ◽  
A Saeed ◽  
S Masood ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Aflatoxin B1 ◽  
Daily Intake ◽  
Total Aflatoxin ◽  
Allowable Limit ◽  
Layer Chromatography ◽  
Feed Samples ◽  
Lahore City

The objective of this study was to estimate total aflatoxin in bird feed. Total 50 birds feed samples were collected from different markets and birds shops in Lahore. Total aflatoxin B1, B2, G1, and G2 were estimated using the Thin Layer Chromatography (TLC) method. The daily intake of aflatoxin in bird's feed is a 300ppb threshold by FDA. Total 50 samples were examined, among them 22% were contaminated with aflatoxin B1 and B2 and the rest of 40% samples were contaminated only with aflatoxin B1. Aflatoxin G1 and G2 were not detected in any feed. The results showed that 42% of samples were contaminated within the permissible limit, 20% were unfit due to having above the allowable limit, and 38% were fit, which did not show any aflatoxin. The current study has demonstrated the easy way of providing the determination of aflatoxin in bird’s feed. Bangladesh J. Sci. Ind. Res.56(4), 249-254, 2021

scholarly journals Prevalence of Aflatoxin B1 and B2 in Poultary Feed

2014 ◽  
Vol 9 ◽  
pp. 109-112
Author(s):  
Sita R. Aryal ◽  
Durga Karki
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Aflatoxin B1 ◽  
Rainy Season ◽  
Winter Season ◽  
Poultry Feed ◽  
Permissible Level ◽  
Aflatoxin B2 ◽  
Layer Chromatography ◽  
Feed Samples

A total of 65 poultry feed samples were examined for the detection of aflatoxin (aflatoxin B1 and aflatoxin B2) using thin layer chromatography (TLC). Samples were collected from Chitwan and Kavrepalanchock districts. Out of those samples examined a total of 49 (75.38%) samples were found positive. Out of 49 (75.38%) samples positive, 42 (85.71%) samples were found positive both with aflatoxin B1 and B2 where as five (10.20%) samples were positive only with aflatoxin B1 and two (4.08%) samples were positive only with aflatoxin B2. Among them 13 (20%) samples were found positive having aflatoxin above permissible level. The concentration of aflatoxin in positive samples ranged from trace to 366 ppb (366 μg/kg). Likewise, out of 52 samples examined in rainy season, 40 samples (76.92%) were found positive where as out of 13 samples examined in winter season 9 (69.23%) were found positive.Nepal Agric. Res. J. Vol. 9, 2009, pp. 109-112DOI: http://dx.doi.org/10.3126/narj.v9i0.11648

Rapid Thin Layer Chromatographic Determination of Patulin, Citrinin, and Af latoxin in Apples and Pears, and Their Juices and Jams

1983 ◽  
Vol 66 (1) ◽  
pp. 85-91 ◽  
Author(s):  
Alberto Gimeno ◽  
Maria Ligia Martins
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Chemical Reactions ◽  
Aflatoxin B1 ◽  
Detection Method ◽  
Limit Of Detection ◽  
Chromatographic Determination ◽  
Layer Chromatography ◽  
Spray Reagents

Abstract A method is described to determine the mycotoxins patulin, citrinin, and aflatoxins in apples and pears and their juices and jams. The mycotoxins are extracted with a mixture of acetonitrile and 4% aqueous KG (9 +1). The extract is cleaned up with water and then acidified, and the toxins are recovered with chloroform and separated by thin layer chromatography. Toxin identity is confirmed with various developing solvents, spray reagents, and chemical reactions, and then quantitated by the limit of detection method. The minimum detectable concentrations of the mycotoxins are patulin, 120-130 μg/kg; citrinin, 30-40 μg/kg; aflatoxin B1 or G1 , 2-2.8 μg/kg; af latoxin B2 or G2,2 μg/kg.

Determination of Buquinolate in Chicken Feeds by Thin Layer Chromatography and Fluorometry

1967 ◽  
Vol 50 (2) ◽  
pp. 264-268
Author(s):  
H Borfitz ◽  
J Para ◽  
J V Stickles ◽  
G B Ginther ◽  
B C Southworth
Keyword(s):  
Standard Deviation ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Chloroform Extract ◽  
Feed Additives ◽  
Reference Standard ◽  
Solvent Systems ◽  
Layer Chromatography ◽  
Feed Samples

Abstract A procedure has been developed for determining buquinolate (ethyl 4-hydroxy- 6,7- diisobutoxy - 3 - quinolinecarboxylate) in finished chicken feeds. In the procedure, a chloroform extract of the feed is concentrated to a small known volume, separated from background substances by thin layer chromatography on silica gel (two solvent systems), eluted from the plate, and compared fluorometrically with reference standard buquinolate. No background interference was found from 157 feed samples investigated or from 11 common feed additives. Recovery experiments on 31 samples at levels ranging from 0.0044 to 0.0143% buquinolate averaged 99.7% with a standard deviation of 3.3. The method is equally applicable to both fresh and aged feeds. This method was studied collaboratively, results were good, and the method is recommended for adoption as official, first action

Extraction and Thin Layer Chromatography of Aflatoxin Bi in Mixed Feeds

1983 ◽  
Vol 66 (3) ◽  
pp. 582-586
Author(s):  
Gail M Shannon ◽  
Odette L Shotwell ◽  
William F Kwolek
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Aflatoxin B1 ◽  
Methylene Chloride ◽  
Relative Standard ◽  
Mixed Feeds ◽  
Layer Chromatography ◽  
Commercial Turkey ◽  
Different Levels

Abstract A method was developed for the determination of aflatoxin B1 in commercially prepared feeds. The method incorporates methylene chloride and citric acid solution extraction, cleanup on a small silica gel column, and thin layer chromatography for quantitation. Commercial turkey starter, catfish chow, medicated pig starter, broiler finisher, rabbit chow, horse feed, rat chow, and dog chow were investigated. The feeds were spiked with naturally contaminated corn at 4 different levels of aflatoxin B1 (16-130 μg/kg). Three assays were run on each of the 32 combinations of feed and levels of aflatoxin. Mean recoveries were 85.9-92.8% at levels of 16.5,32.9,65.8, and 131.6 μg/kg. The relative standard deviation per assay was 18.6%. This method is more rapid and less involved than most previously published methods for mixed feeds.

scholarly journals Occurrence of Aflatoxins in Retail Kola Nuts in Ibadan Metropolis

2021 ◽  
pp. 1-5
Author(s):  
O. O. Akande ◽  
O. O. Kolawole ◽  
A. A. Afolabi ◽  
S. A. Okewole ◽  
L. O. Oyekunle
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Study Design ◽  
Aflatoxin B1 ◽  
Spectrophotometric Method ◽  
Layer Chromatography

Aim: This study was designed to determine Aflatoxins present in kola nuts sold in Ibadan metropolis. Study design: Kola nuts were randomly purchased from different markets in Ibadan, Oyo State. Methodology: Thin layer chromatography (TLC) and spectrophotometric method were used for the determination of aflatoxins in the kola nuts samples. Results: All the samples collected were contaminated with aflatoxins. Aflatoxin B1 and B2 were present in the kola nuts samples ranging from 9.73 - 25.43 μg/kg and 10.90 - 29.67 μg/kg respectively. Conclusion: The levels of aflatoxins in the sampled kola nuts were mostly above recommended tolerable limits. It is imperative to enlighten stakeholders on possible ways of handling kola nuts towards reducing the aflatoxin incidences to the barest minimum in order to safeguard the health of the populace.

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