scholarly journals Enzymatic Hydrolysis of Rice Straw to Fermentable Sugar: Kinetic Study

2014 ◽  
Vol 27 (2) ◽  
pp. 20-24
Author(s):  
Pradip Saha ◽  
MR Khan ◽  
TK Deb ◽  
S Majumdar ◽  
F Alam ◽  
...  
Keyword(s):  
Rice Straw ◽  
Chemical Engineering ◽  
Heterogeneous Reaction ◽  
Glucose Production ◽  
Modern World ◽  
Absolute Deviation ◽  
New Era ◽  
Hydrolysis Of Cellulose ◽  
Promising Source ◽  
Hydrolysis Of

As the gradual up-growing trend of industrialization and urbanization leading steady increment of demand of energy; eco-friendly, bio degradable, cost competitive and promising source of energy with high sustainability is toughly needed for the new era of modern world. Hydrolysis of cellulose by cellulase enzymes is a vital candidate for this option. It is a solid-liquid heterogeneous reaction; strongly affected by the non-reaction resistances caused most notably by the crystalline structure; reaction environment parameters as temperature, pH, characteristics of enzyme, cell & substrate loading and hence must have to be defined for specific enzyme-substrate amalgamation. In this present investigation, glucose was produced from rice straw using cellulytic enzyme pseudomonas sp., isolated from municipal solid waste. Glucose yield was found to increase as the rice straw particle size decreased from 0.5 mm to 45 ?m, while the optimal temperature and pH were found within the range of 30°C and 7.0 respectively. The concentration and rate of glucose production was observed to depend on pretreatment of rice straw, substrate concentration and enzyme loading. A kinetic model rate expression has been developed for such a process based on the Michaelis – Mentens and Line weaver–Burk approach. Comparison between the experimental data and those predicted from the rate model indicate good agreement with a mean absolute deviation of about 0.304916. DOI: http://dx.doi.org/10.3329/jce.v27i2.17778 Journal of Chemical Engineering, IEB Vol. ChE. 27, No. 2, December 2012: 20-24

scholarly journals Production of Sugar From Sweet Sorghum Stems with Hydrolysis Method Using Trichoderma viride

2021 ◽  
Vol 13 (1) ◽  
pp. 121-127
Author(s):  
Melycorianda Hubi Ndapamuri ◽  
Maria Marina Herawati ◽  
V Irene Meitiniarti
Keyword(s):  
Trichoderma Viride ◽  
Glucose Production ◽  
Cellulose Content ◽  
Hydrolysis Time ◽  
Glucose Yield ◽  
Hydrolysis Method ◽  
Hydrolysis Process ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of ◽  
Bagasse Waste

Sorghum stem bagasse waste is one of the materials with high cellulose content. It can be utilized in glucose production through enzymatic hydrolysis of cellulose by Trichoderma viride. This study aims to determine sorghum stem bagasse’s potential in producing glucose, assessing the time and concentration of sorghum stem bagasse in the hydrolysis process to produce glucose optimally and following SNI. Hydrolysis was carried out using a concentration of 5%, 8%, and 11% sorghum stem bagasse for 10, 15, and 20 days. The results showed that sorghum stem bagasse waste could produce glucose with an average glucose yield of 10.09% to 24.40 %. There is a tendency that increasing substrate concentration and hydrolysis time will increase the yield of liquid glucose. The treatment of 5% concentration of sorghum stem bagasse with a long hydrolysis time of 10 days can produce the highest liquid glucose, namely 24.40% with total dissolved solids of 7.40% Brix, the ash content of 0.26%, but 47.54% water content has not met SNI standards. 

Kinetics of Enzymatic Hydrolysis of Cellulose Using Aspergillus niger

2015 ◽  
Vol 1101 ◽  
pp. 294-298
Author(s):  
Akida Mulyaningtyas ◽  
Ratna Ningsih ◽  
Siti Syamsiah ◽  
Sarto ◽  
Wahyudi Budi Sediawan
Keyword(s):  
Enzymatic Hydrolysis ◽  
Glucose Production ◽  
Mass Reduction ◽  
Monod Equation ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Process ◽  
Hydrolysis Of Cellulose ◽  
The Relationship ◽  
Kinetics Of ◽  
Hydrolysis Of

This study aimed to analyze the kinetics of enzymatic hydrolysis of cellulose. Cellulase enzyme was obtained fromAspergillusnigergrown on filter paper and the hydrolysis process was carried out in solid state. The kinetic reviewed here was the relationship between glucose production and cellulose mass reduction to optimize the parameters of Monod equation at various moisture levels.

Immobilized Nanoparticles-Mediated Enzymatic Hydrolysis of Cellulose for Clean Sugar Production: A Novel Approach

2019 ◽  
Vol 15 (3) ◽  
pp. 296-303 ◽  
Author(s):  
Swapnil Gaikwad ◽  
Avinash P. Ingle ◽  
Silvio Silverio da Silva ◽  
Mahendra Rai
Keyword(s):  
Catalytic Activity ◽  
Enzymatic Hydrolysis ◽  
Immobilized Enzyme ◽  
Free Enzyme ◽  
Magnetic Nanomaterials ◽  
Sugar Production ◽  
Cellulase Enzyme ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

Background: Enzymatic hydrolysis of cellulose is an expensive approach due to the high cost of an enzyme involved in the process. The goal of the current study was to apply magnetic nanomaterials as a support for immobilization of enzyme, which helps in the repeated use of immobilized enzyme for hydrolysis to make the process cost-effective. In addition, it will also provide stability to enzyme and increase its catalytic activity. Objective: The main aim of the present study is to immobilize cellulase enzyme on Magnetic Nanoparticles (MNPs) in order to enable the enzyme to be re-used for clean sugar production from cellulose. Methods: MNPs were synthesized using chemical precipitation methods and characterized by different techniques. Further, cellulase enzyme was immobilized on MNPs and efficacy of free and immobilized cellulase for hydrolysis of cellulose was evaluated. Results: Enzymatic hydrolysis of cellulose by immobilized enzyme showed enhanced catalytic activity after 48 hours compared to free enzyme. In first cycle of hydrolysis, immobilized enzyme hydrolyzed the cellulose and produced 19.5 ± 0.15 gm/L of glucose after 48 hours. On the contrary, free enzyme produced only 13.7 ± 0.25 gm/L of glucose in 48 hours. Immobilized enzyme maintained its stability and produced 6.15 ± 0.15 and 3.03 ± 0.25 gm/L of glucose in second and third cycle, respectively after 48 hours. Conclusion: This study will be very useful for sugar production because of enzyme binding efficiency and admirable reusability of immobilized enzyme, which leads to the significant increase in production of sugar from cellulosic materials.

In Situ Microscopy for In-line Monitoring of the Enzymatic Hydrolysis of Cellulose

2013 ◽  
Vol 85 (17) ◽  
pp. 8121-8126 ◽  
Author(s):  
Britta Opitz ◽  
Andreas Prediger ◽  
Christian Lüder ◽  
Marrit Eckstein ◽  
Lutz Hilterhaus ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

scholarly journals Generation of highly amenable cellulose-Iβ via selective delignification of rice straw using a reusable cyclic ether-assisted deep eutectic solvent system

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chiranjeevi Thulluri ◽  
Ravi Balasubramaniam ◽  
Harshad Ravindra Velankar
Keyword(s):  
Enzymatic Hydrolysis ◽  
Rice Straw ◽  
Deep Eutectic Solvent ◽  
Solvent System ◽  
Cyclic Ether ◽  
Cellulolytic Enzymes ◽  
Ammonium Bromide ◽  
Selective Delignification ◽  
Cellulose Iβ ◽  
Hydrolysis Of

AbstractCellulolytic enzymes can readily access the cellulosic component of lignocellulosic biomass after the removal of lignin during biomass pretreatment. The enzymatic hydrolysis of cellulose is necessary for generating monomeric sugars, which are then fermented into ethanol. In our study, a combination of a deep eutectic (DE) mixture (of 2-aminoethanol and tetra-n-butyl ammonium bromide) and a cyclic ether (tetrahydrofuran) was used for selective delignification of rice straw (RS) under mild conditions (100 °C). Pretreatment with DE-THF solvent system caused ~ 46% delignification whereas cellulose (~ 91%) and hemicellulose (~ 67%) recoveries remained higher. The new solvent system could be reused upto 10 subsequent cycles with the same effectivity. Interestingly, the DE-THF pretreated cellulose showed remarkable enzymatic hydrolysability, despite an increase in its crystallinity to 72.3%. Contrary to conventional pretreatments, we report for the first time that the enzymatic hydrolysis of pretreated cellulose is enhanced by the removal of lignin during DE-THF pretreatment, notwithstanding an increase in its crystallinity. The current study paves way for the development of newer strategies for biomass depolymerization with DES based solvents.

Enhanced Hydrolysis of Cellulose to Reducing Sugars on Kaolinte Clay Activated by Mineral Acid

2021 ◽  
Author(s):  
Yuxiao Dong ◽  
Dongshen Tong ◽  
Laibin Ren ◽  
Xingtao Chen ◽  
Hao Zhang ◽  
...  
Keyword(s):  
Reducing Sugars ◽  
Mineral Acid ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of
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