scholarly journals Molecular detection of Pasteurella multocida Type B causing haemorrhagic septicemia in cattle and buffaloes of Bangladesh

2016 ◽  
Vol 27 (2) ◽  
pp. 175-179 ◽  
Author(s):  
MS Ara ◽  
MT Rahman ◽  
M Akhtar ◽  
M Rahman ◽  
KHMNH Nazir ◽  
...  
Keyword(s):  
Morphological Study ◽  
Pasteurella Multocida ◽  
Molecular Detection ◽  
Vaccine Candidate ◽  
Clinical Samples ◽  
Effective Vaccine ◽  
Type B ◽  
Chain Reaction ◽  
Specific Primers ◽  
Polymerase Chain

Hemorrhagic septicemia (HS) is an acute septicemic disease that primarily affects cattle and buffaloes. The disease is caused by Pasteurella multocida sero types B:2 and E:2. The objective of this study was to isolate P. multocida from clinical cases and to confirm its identity using polymerase chain reaction (PCR) based approach. Clinical samples of two suspected cases of haemorrhagic septicemia of cattle and buffalo from Mymensingh and Rajshahi districts respectively were collected. Two isolates were isolated from these suspected cases and primarily identified as P. multocida based on morphological study, staining properties, and cultural and biochemical characteristics. The isolates were confirmed initially as P. multocida at genus level by PCR using genus specific primers. Later, the isolates were confirmed as P. multocida type B, the causal agent of haemorrhagic septicemia, by PCR with primers specific for P. multocida type B. These isolated organisms can be used as vaccine candidate for the production of effective vaccine against haemorrhagic septicemia.Progressive Agriculture 27 (2): 175-179, 2016

scholarly journals Prevalence and molecular detection of shiga toxin producing Escherichia coli from diarrheic cattle

2016 ◽  
Vol 14 (1) ◽  
pp. 63-68 ◽  
Author(s):  
MM Akter ◽  
S Majumder ◽  
KH MNH Nazir ◽  
M Rahman
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Molecular Detection ◽  
Chain Reaction ◽  
Specific Primers ◽  
Fecal Samples ◽  
E Coli ◽  
Uremic Syndrome ◽  
Polymerase Chain ◽  
Positive Isolate

Shiga toxin-producing Escherichia coli (STEC) are zoonotically important pathogen which causes hemorrhagic colitis, diarrhea, and hemolytic uremic syndrome in animals and humans. The present study was designed to isolate and identify the STEC from fecal samples of diarrheic cattle. A total of 35 diarrheic fecal samples were collected from Bangladesh Agricultural University (BAU) Veterinary Teaching Hospital. The samples were primarily examined for the detection of E. coli by cultural, morphological and biochemical characteristics, followed by confirmation of the isolates by Polymerase Chain Reaction (PCR) using gene specific primers. Later, the STEC were identified among the isolated E. coli through detection of Stx-1 and Stx-2 genes using duplex PCR. Out of 35 samples, 25 (71.43%) isolates were confirmed to be associated with E. coli, of which only 7 (28%) isolates were shiga toxin producers, and all of them were positive for Stx-1. However, no Stx-2 positive isolate could be detected. From this study, it may be concluded that cattle can act as a reservoir of STEC which may transmit to human or other animals.J. Bangladesh Agril. Univ. 14(1): 63-68, June 2016

scholarly journals Molecular identification by multiplex polymerase chain reaction of Pasteurella multocida in cattle and buffaloes in Baghdad

2014 ◽  
Vol 38 (1) ◽  
pp. 99-106
Author(s):  
Ihab G. M. AL-Shemmari
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Pcr ◽  
Multiplex Polymerase Chain Reaction ◽  
Pasteurella Multocida ◽  
Type B ◽  
Chain Reaction ◽  
Blood Samples ◽  
Nasal Swabs ◽  
Polymerase Chain ◽  
B 31

The aim of this study was to identify pasteurella multocida and their types by PCR in cattle’s and buffaloesi bagdad from March to August 2012 on 204 animals , including 102 cattle and 102 buffaloes at slaughter houses from Baghdad .Blood samples and nasal swaps were collected , before slaughtering and lung tissues of slaughtered animal , and from 54 clinically suspected cases of pasteurellosis , including 27 bovines ,and 27 buffaloes the samples taken included blood and nasal swabs . Pasteurellamultocida were isolated from 94 animals include 49 cattle 45 buffaloes. The typing of the isolates by multiplex PCR for genotyping Pasteuerllamultocida revealed 93 isolates of type B , 31 from cattle and 62 from buffaloes ,and 81 isolates of type A , 55 from cattle and 26 from buffaloes .

scholarly journals First detection of Pasteurella multocida type B:2 in Hungary associated with systemic pasteurellosis in backyard pigs

2015 ◽  
Vol 63 (2) ◽  
pp. 141-156 ◽  
Author(s):  
Barbara Ujvári ◽  
Levente Szeredi ◽  
László Pertl ◽  
Gergely Tóth ◽  
Károly Erdélyi ◽  
...  
Keyword(s):  
Small Area ◽  
Pasteurella Multocida ◽  
Sequence Type ◽  
Type B ◽  
Chain Reaction ◽  
Haemorrhagic Septicaemia ◽  
Gene Typing ◽  
Polymerase Chain ◽  
Serotype B ◽  
Backyard Pigs

This is the first report of Pasteurella multocida type B in Hungarian pigs. This disease was observed in backyard-raised pigs in three households within a small area. Neither the source of the infection nor the epidemiological connection between any of the premises could be determined. The most consistent lesion was dark red discolouration of the skin of the ventral neck and brisket, with accompanying oedema and haemorrhages. The morbidity was low and lethality relatively high, with three dead (50%) and two euthanised (33%) out of six affected animals. A total of three isolates of P. multocida (P55, P56 and P57) were cultured from these cases and examined in detail. These were identified as P. multocida ssp. multocida biovar 3. All were toxA negative and belonged to serotype B:2. Multilocus sequence typing was used to assign these to a new sequence type (ST61) that is closely related to other haemorrhagic septicaemia causing strains of P. multocida regardless of the host. M13 polymerase chain reaction and virulence-associated gene typing also show that type B strains form a highly homogeneous, distinct phylogenic group within P. multocida.

scholarly journals Isolation and Toxin Typing of Clostridium Perfringens From Sheep, Goats, and Cattle in Fars Province, Iran

2020 ◽  
Vol 8 (3) ◽  
pp. 89-93
Author(s):  
Masoumeh Hayati ◽  
Mehrdad Shamseddini ◽  
Yahya Tahamtan ◽  
Safar Sadeghzadeh ◽  
Mohsen Manavian ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Fars Province ◽  
Type B ◽  
Chain Reaction ◽  
Specific Primers ◽  
Defined Media ◽  
Different Types ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Published Research

Background: Clostridium perfringens is an important anaerobic bacterium found in the intestine of some livestock. It is concerned with the etiology of some diseases including enterotoxaemia. Various diseases are caused by different types of C. perfringens. Nonetheless, there is no published research on molecular typing and distribution of this pathogenic microorganism in Fars province. Objectives: Accordingly, our study focused on the isolation and toxin typing of C. perfringens from sheep, cattle, and goats in different parts of Fars province by the culture and the polymerase chain reaction (PCR) method. Materials and Methods: Approximately 459 fecal samples were collected and cultured on defined media for the isolation of C. perfringens. The confirmed isolates were genotyped by the PCR method using specific primers. Results: C. perfringens was isolated from 30.93% of the total samples. The results of toxin typing showed a total of 76 (54%), 13 (9%), 30 (21%), and 23 (16%) isolates as types A, B, C, and D, respectively. Conclusion: Our results indicated that C. perfringens type A was the most common type in sheep, cattle, and goats while the lowest number of isolates belonged to type B. Finally, the isolation of C. perfringens and toxin typing increase our knowledge of the epidemiology of these diseases and can help in the vaccine industry and better controlling related diseases.

scholarly journals Polymerase Chain Reaction Detection of Pasteurella multocida Type B:2 in Mice Following Oral Inoculation

2013 ◽  
Vol 8 (3) ◽  
pp. 493-501 ◽  
Author(s):  
Faez Firdaus Je ◽  
Abdinasir Yusuf Osma ◽  
Lawan Adamu ◽  
Mohd Syamil Moh ◽  
Abdul Rahman Oma ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Pasteurella Multocida ◽  
Type B ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Oral Inoculation ◽  
Polymerase Chain

scholarly journals Molecular detection of Grapevine fanleaf Virus by the isolation of ssRNA and dsRNA from Xiphinema index

2017 ◽  
Vol 1 ◽  
pp. 100
Author(s):  
Afsasneh Delpasand Khabbazi ◽  
Nemat Sokhandan Bashir ◽  
Ebrahim Zahedi Asl ◽  
Saber Delpasand Khabbazi
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Detection ◽  
Virus Detection ◽  
Detection Time ◽  
Grapevine Fanleaf Virus ◽  
Chain Reaction ◽  
Specific Primers ◽  
Xiphinema Index ◽  
Viral Genes ◽  
Polymerase Chain

Xiphinema index is an important grapevine pathogen nematode which also vectors Grapevine fanleaf virus. The viral genes involved in transmission by the vector nematode are mapped to the C-terminal residues of RNA2-encoded polyprotein. To recognize viruliferous nematodes, there are some serological and molecular methods. In this study, we extract RNA and dsRNA of the virus, then Reverse transcription-polymerase Chain Reaction was done with virus specific primers to detect virus in its vector. The virus was detected by visualizing the desired 350 and 750 bp gene fragments in electrophoresis. This study reduces the virus detection time to only couple of hours with least imposed charges, and could be employed in transmission experiments as well. 

scholarly journals Molecular detection of β-lactamase genes in Klebsiella pneumoniae and Escherichia coli isolated from different clinical sources

2022 ◽  
Vol 67 (4) ◽  
pp. 170-180
Author(s):  
Kamal Ismael Bakr ◽  
Sherko Muhammed Abdul-Rahman ◽  
Rebwar Muhammad Hamasalih
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Molecular Detection ◽  
Multidrug Resistant ◽  
Clinical Samples ◽  
Chain Reaction ◽  
E Coli ◽  
Recent Emergence ◽  
Polymerase Chain ◽  
Pcr Test

The rising occurrence of infections generated by Escherichia coli and Klebsiella pneumoniae that produce extended-spectrum β-lactamase (ESBL) is reason for concern. Due to the recent emergence of multidrug-resistant microorganisms that develop ESBL. The purpose of this work was to detect the ESBLs in clinical isolates of E. coli and K. pneumoniae. 118 samples of E. coli and 63 isolates of K. pneumoniae were collected from clinical samples. Polymerase chain reaction was used to detect β-lactamase genes (i.e., blaTEM, blaSHV, and blaCTX-M). Phenotypic detection revealed that 48.31% and 85.19% of E. coli and K. pneumoniae produced ESBLs, respectively. Whereas screening of ESBL genes in both bacteria employing a multiplex PCR test revealed that 24.58% of the ESBL-producing E. coli strains contained blaTEM, 50.85% contained blaSHV, and 32.2% contained blaCTX-M. Nevertheless, in K. pneumoniae, 40.74% blaTEM, 35.19% blaSHV, and 64.81% blaCTX-M genes were present. Antimicrobial resistance profiles of E. coli and K. pneumoniae isolates to twenty antibiotics were observed to vary significantly. Additionally, it was determined that the majority of E. coli and K. pneumoniae isolates were multidrug resistant (MDR). Additionally, 80.51% of E. coli isolates were resistant to the AMC antibiotic, while 0.00% were resistant to IPM and MEM. From the other hand, the resistant proportion of K. pneumoniae isolates was heterogeneous, ranging from 69.84% against CAZ to 0.00% against CIP and G antibiotics. The blaSHV gene was the most widespread among different forms of ESBLs in E. coli, but the most common gene in K. pneumoniae isolates was blaCTX-M (64.81%).

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