scholarly journals Isolation and Toxin Typing of Clostridium Perfringens From Sheep, Goats, and Cattle in Fars Province, Iran

2020 ◽  
Vol 8 (3) ◽  
pp. 89-93
Author(s):  
Masoumeh Hayati ◽  
Mehrdad Shamseddini ◽  
Yahya Tahamtan ◽  
Safar Sadeghzadeh ◽  
Mohsen Manavian ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Fars Province ◽  
Type B ◽  
Chain Reaction ◽  
Specific Primers ◽  
Defined Media ◽  
Different Types ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Published Research

Background: Clostridium perfringens is an important anaerobic bacterium found in the intestine of some livestock. It is concerned with the etiology of some diseases including enterotoxaemia. Various diseases are caused by different types of C. perfringens. Nonetheless, there is no published research on molecular typing and distribution of this pathogenic microorganism in Fars province. Objectives: Accordingly, our study focused on the isolation and toxin typing of C. perfringens from sheep, cattle, and goats in different parts of Fars province by the culture and the polymerase chain reaction (PCR) method. Materials and Methods: Approximately 459 fecal samples were collected and cultured on defined media for the isolation of C. perfringens. The confirmed isolates were genotyped by the PCR method using specific primers. Results: C. perfringens was isolated from 30.93% of the total samples. The results of toxin typing showed a total of 76 (54%), 13 (9%), 30 (21%), and 23 (16%) isolates as types A, B, C, and D, respectively. Conclusion: Our results indicated that C. perfringens type A was the most common type in sheep, cattle, and goats while the lowest number of isolates belonged to type B. Finally, the isolation of C. perfringens and toxin typing increase our knowledge of the epidemiology of these diseases and can help in the vaccine industry and better controlling related diseases.

scholarly journals Prevalence of ces and cytk Genes of Bacillus cereus Isolated From Raw Milk in Tabriz, Iran

2020 ◽  
Vol 8 (3) ◽  
pp. 76-79
Author(s):  
Mahtab Hamidpour ◽  
Saman Mahdavi
Keyword(s):  
Bacillus Cereus ◽  
Bacterial Contamination ◽  
Dairy Product ◽  
Foodborne Pathogen ◽  
Food Poisoning ◽  
Raw Milk ◽  
Specific Primers ◽  
Different Types ◽  
Pcr Method ◽  
Polymerase Chain

Background: Bacillus cereus is a gram-positive and spore-forming bacterium which is widespread in nature. It also has been known as a major foodborne pathogen that often plays a role in the contamination of ready-to-eat and dairy products. It causes two different types of food poisoning in human: the diarrheal type and the emetic type. Objective: The current study was planned to determine the prevalence of ces and cytk genes of Bacillus cereus isolated from raw milk in Tabriz, Iran. Materials and Methods: In this study, 40 B. cereus strains isolated from cow raw milk, that had already been identified phenotypically, were assessed for molecular confirmation by polymerase chain reaction (PCR) method. Then, they were evaluated for presence of ces and cytK genes by specific primers. Results: Of 40 B. cereus strains, 39 strains were confirmed molecularly. The frequency of cytK and ces genes was reported 38 (97.43%) and 0 (0%), respectively. Conclusion: The results of present study showed that B. cereus strains isolated from raw milk had high potential in causing diarrhea poisoning. Therefore, using procedures to reduce the bacterial contamination during the processing of dairy product is essential.

scholarly journals Mite Sarcoptes scabiei Varieties Hominis in South Sumatra: Specific Identification and Comparative Study

2020 ◽  
Vol 8 (A) ◽  
pp. 938-942
Author(s):  
Yessi Arisandi ◽  
Chairil Anwar ◽  
Salni Salni ◽  
Dadang Hikmah Purnama ◽  
Novrikasari Novrikasari ◽  
...  
Keyword(s):  
Deoxyribonucleic Acid ◽  
Sarcoptes Scabiei ◽  
Chain Reaction ◽  
Specific Primers ◽  
Specific Identification ◽  
16S Gene ◽  
Dna Strands ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Relationship Of

BACKGROUND: Sarcoptes scabiei mites have more than 15 genetically diverse varieties from various hosts. Identification of S. scabiei mite varieties hominis as an intervention in its prevention is still rarely done. AIM: This study aimed to observe the genetic relationship of the mite S. scabiei varieties hominis compare to the parasite S. scabiei varieties hominis in other regions. METHODS: This research used polymerase chain reaction (PCR) and sequencing methods with 16S gene-specific primers. From 32 S. scabiei samples, 22 samples were identified as varieties hominis that was marked by the appearance of the band at 132 bp. RESULTS: S. scabiei mites hominis varieties from South Sumatra (Yessi Scabies A2 and Yessi Scabies B3) have similarities with deoxyribonucleic acid (DNA) strands with S. scabiei hominis varieties from China (KJ781377 and KJ781376). In contrast, Yessi Scabies A1 has similarities with DNA strands with mite S. scabiei varieties hominis from Australia (AY493402). Still, all the DNA strands, this research is different from S. scabiei mites DNA strands hominis from Panama and Pakistan. CONCLUSION: The PCR method is advantageous and specific in identifying mites S. scabiei hominis varieties, the cause of scabies in humans.

scholarly journals Eliminasi Endosimbion Wolbachia sp. pada Nyamuk Aedes albopictus dengan Antibiotik Tetrasiklin

2021 ◽  
pp. 171-178
Author(s):  
Endang Srimurni Kusmintarsih ◽  
Darsono Darsono ◽  
Edy Riwidiharso ◽  
Rokhmani Rokhmani ◽  
Trisnowati Ambarningrum ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Hemorrhagic Fever ◽  
Survey Method ◽  
Chain Reaction ◽  
Specific Primers ◽  
Tetracycline Antibiotics ◽  
Sugar Feeding ◽  
Pcr Method ◽  
Primary Vector ◽  
Polymerase Chain

Dengue Hemorrhagic Fever (DHF) is a disease caused by the dengue virus which is transmitted through the bite of the Aedes mosquito. Aedes aegypti, which is known as the primary vector of dengue virus, is naturally not infected by Wolbachia sp. endosymbiont, while Ae. albopictus which is a secondary vector naturally infected with Wolbachia sp. The Wolbachia sp. known to inhibit the transmission of Dengue virus, to study the mechanism, it is necessary to eliminate Wolbachia sp. from Ae. albopictus, then infects the Ae. albopictus with the Dengue virus. The aim of the study was to determine the ability of tetracycline antibiotics to eliminate Wolbachia sp. from the Ae. albopictus mosquito. Ae. albopictus eggs was obtained in the Ciamis area by survey method using ovitrap which was installed outside the house. The Ae. albopictus eggs are then incubated in the laboratory and reared until they become adult mosquitoes. Mosquitoes were treated with sugar feeding which had been given tetracycline 0.25mg/ml every two days alternated with blood feed. Detection of the presence of Wolbachia sp. on mosquitoes carried out in first to third generations by the polymerase chain reaction (PCR) method using Wsp-specific primers. The results showed that the first to third generation mosquitoes were still infected with Wolbachia sp. This shows that the dose of tetracycline antibiotics used has not been able to eliminate Wolbachia sp. from the Ae. albopictus mosquito.

A Multiplex PCR Method for the Identification of Listeria spp. and Listeria monocytogenes in Dairy Samples

1995 ◽  
Vol 58 (8) ◽  
pp. 867-872 ◽  
Author(s):  
LIEVE M. F. HERMAN ◽  
HERMAN F. M. DE RIDDER ◽  
GEERTRUI M. M. VLAEMYNCK
Keyword(s):  
Listeria Monocytogenes ◽  
Multiplex Pcr ◽  
Gene Sequence ◽  
Chain Reaction ◽  
Specific Primers ◽  
16S Rrna Sequence ◽  
Bacteriological Method ◽  
Agarose Electrophoresis ◽  
Pcr Method ◽  
Polymerase Chain

A multiplex polymerase chain reaction (PCR) method for the identification of Listeria spp. and Listeria monocytogenes has been developed. For the identification of Listeria spp. two specific primers were derived from the 16S rRNA sequence. For the identification of Listeria monocytogenes specific primers were derived from the gene sequence of the invasion-associated protein. The two amplified DNA fragments, respectively 1003 bp and 593 bp, were separated by agarose electrophoresis. The results obtained by the multiplex PCR method were in agreement with those obtained by the classical bacteriological method for the 64 dairy samples with presumptive positives.

scholarly journals Molecular detection of Pasteurella multocida Type B causing haemorrhagic septicemia in cattle and buffaloes of Bangladesh

2016 ◽  
Vol 27 (2) ◽  
pp. 175-179 ◽  
Author(s):  
MS Ara ◽  
MT Rahman ◽  
M Akhtar ◽  
M Rahman ◽  
KHMNH Nazir ◽  
...  
Keyword(s):  
Morphological Study ◽  
Pasteurella Multocida ◽  
Molecular Detection ◽  
Vaccine Candidate ◽  
Clinical Samples ◽  
Effective Vaccine ◽  
Type B ◽  
Chain Reaction ◽  
Specific Primers ◽  
Polymerase Chain

Hemorrhagic septicemia (HS) is an acute septicemic disease that primarily affects cattle and buffaloes. The disease is caused by Pasteurella multocida sero types B:2 and E:2. The objective of this study was to isolate P. multocida from clinical cases and to confirm its identity using polymerase chain reaction (PCR) based approach. Clinical samples of two suspected cases of haemorrhagic septicemia of cattle and buffalo from Mymensingh and Rajshahi districts respectively were collected. Two isolates were isolated from these suspected cases and primarily identified as P. multocida based on morphological study, staining properties, and cultural and biochemical characteristics. The isolates were confirmed initially as P. multocida at genus level by PCR using genus specific primers. Later, the isolates were confirmed as P. multocida type B, the causal agent of haemorrhagic septicemia, by PCR with primers specific for P. multocida type B. These isolated organisms can be used as vaccine candidate for the production of effective vaccine against haemorrhagic septicemia.Progressive Agriculture 27 (2): 175-179, 2016

scholarly journals Polymerase chain reaction (PCR) for the rapid detection of Salmonella in desiccated coconut (DC)

CORD ◽  
2007 ◽  
Vol 23 (2) ◽  
pp. 7
Author(s):  
Jayaratne, D.L.
Keyword(s):  
Polymerase Chain Reaction ◽  
Genomic Dna ◽  
Chain Reaction ◽  
Specific Primers ◽  
Routine Testing ◽  
Cultural Methods ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Low Levels ◽  
M1 Type

This study was carried out to develop a polymerase chain reaction (PCR) protocol for the detection of Salmonella in desiccated coconut (DC). For the PCR detection of Salmonella, genomic DNA was extracted using the ‘boiling lyses’ method and the reaction was carried out with Salmonella genus specific primers enabled to amplify 457bp sequence covering invA and invE genes. Samples of DC produced in mills already tested for Salmonella using conventional cultural methods gave identical results with the present PCR method indicating its suitability for adoption in routine testing.  The sensitivity checked using DNA extracted from artificially inoculated DC with serially diluted inoculum of Salmonella M1 type showed that the developed PCR method can be used to detect very low levels of contamination of Salmonella as low as 4 CFU/g in DC.  The method described here reduces the testing and detection time from 6 days to 24 hours ensuring exporters to obtain Salmonella test reports just prior to shipment. 

Analysis of babA, cagE and cagA Genes in Helicobacter pylori from Upper Gastric Patients in the North of Iran

2019 ◽  
Vol 19 (3) ◽  
pp. 274-278 ◽  
Author(s):  
Saba Fakhrieh Asl ◽  
Mehrnaz Pourvahedi ◽  
Ali Mojtahedi ◽  
Mohammad Shenagari
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Diseases ◽  
Specific Primers ◽  
The North ◽  
Different Types ◽  
North Of Iran ◽  
Gastric Biopsies ◽  
Pcr Method ◽  
Non Ulcer Dyspepsia ◽  
H Pylori

Objective:Helicobacter pylori is a Gram-negative bacterium which has a serious effect on up to half of the world’s population and has been related to different gastric diseases. The goal of this study was to assess the frequency of babA, cagE and cagA genotypes among H. pylori strains isolated from gastric biopsies of endoscopic patients in the north of Iran.Methods:The present study was performed on 90 strains of H. pylori isolated from patients with gastric diseases (Gastric ulcer (GU), Duodenal ulcer (DU), Gastritis (G), Non-ulcer dyspepsia (NUD) and Gastric adenocarcinoma (GC)). DNA was extracted from all isolated strains and PCR method was performed to detect the prevalence of babA2, cagE and cagA genes using specific primers.Results:Among 90 samples of H. pylori, babA2, cagE, and cagA genes were detected in 42.2%, 30% and 82.2% of strains respectively. The statistical analysis showed that the prevalence of cagA gene in GU, G, DU, and NUD was significantly higher than other genes. Moreover, cagA, and babA2 genes were significantly more prevalent in GC patients compared to cagE gene. Our isolates exhibited 8 distinct arrangements of virulence patterns. The occurrence of cagA (35.6%) was the most prevalent pattern followed by cagA/babA2 (20%) and cagA/babA2/cagE (14.4%).Conclusion:In summary, as first report from Guilan province in the north of Iran, we showed significant association between the presence of babA2, cagE, and cagA genes in different types of gastric disorders.

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