scholarly journals Factors Affecting the Selection of Callus Cell Lines and the Preparation of the Cell Suspension Culture of Artemisia annua L.

2014 ◽  
Vol 23 (2) ◽  
pp. 157-163 ◽  
Author(s):  
Ch’ng Song Jin ◽  
Chan Lai Keng
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Lines ◽  
Cell Suspension Culture ◽  
Artemisia Annua ◽  
Leaf Explants ◽  
In Vitro Production ◽  
Callus Cell ◽  
Alternative Mode ◽  
Artemisia Annua L

Artemisinin, an important antimalarial drug against multidrug resistant strains of Plasmodium, can be produced in Artemisia annua L. Field production of artemisinin is affected by environmental condition and geographical location. In vitro production via cell suspension culture is an alternative mode and cell line selection is important to ensure sustainable production of biomass and artemisinin. Callus cell lines were derived from the leaf explants of five A. annua clones grown in two different locations in Vietnam. Thirty-four callus cell lines with consistent growth index (GI) were selected from these five clones and were categorized into fast (GI > 20), intermediate (GI 15 - 20) and slow (GI < 15) growing groups. The selected lines were found to have different morphology in term of colour and texture. The callus texture did affect the cell growth of A. annua in which the friable callus type showed faster, consistent and sustainable cell biomass production.D. O. I.http://dx.doi.org/10.3329/ptcb.v23i2.17507Plant Tissue Cult. & Biotech. 23(2): 157-163, 2013  (December)

scholarly journals DIFFERENTIAL EFFECT OF PURIFIED QUERCETIN AND ITS DERIVATIVES FROM IN VITRO CELL SUSPENSION CULTURES OF CAESALPINIA PULCHERRIMA SW. AGAINST SELECTED CANCER CELL LINES AND ITS MODE OF ACTION

2018 ◽  
Vol 8 (5-s) ◽  
pp. 196-208
Author(s):  
JM Aswathy ◽  
Greeshma Murukan ◽  
Bosco Lawarence ◽  
K Murugan
Keyword(s):  
Flow Cytometry ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Lines ◽  
Cell Suspension Culture ◽  
Callus Induction ◽  
Mode Of Action ◽  
Cell Suspension Cultures ◽  
Mcf 7

Tribal people use the floral extract of Caesalpinia pulcherrima to cure liver, stomach and skin prone disorders in traditional Indian medicine. This study aimed to evaluate the effect of purified quercetin and its derivatives from in vitro cell suspension cultures of C. pulcherrima Sw. against SW 480, HeLa, MCF-7 and MCF 10A cell lines and its mode of action. Standard protocol was developed for callus induction using leaf explants. Cytotoxic effect was evaluated against SW 480, HeLa, MCF-7 and MCF 10A cells by MTT assay. Apoptosis was evaluated via Hoechst analysis,  flow cytometry, mitochondrial membrane potential and caspase 3 and 9 expression. 2, 4-D (2.5 mg/l), BAP (2.5 mg/l) + kin (1 mg/ml) was effective for remarkable callus induction. Further, cell suspension culture was established.  Effect of elicitors on cell suspension culture was also carried. Sucrose, ABA and salicylic acid (SA) at different concentrations influenced cell biomass and quercetin synthesis. Cells cultured on the medium fortified with 45 g/L sucrose without ABA/SA showed the highest quercetin content (16.5 mg/g). Quercetin was purified, fractionated by HPLC-DAD and was further analyzed by NMR revealed a major fraction of quercetin (3, 5, 7, 3’, 4’-pentahydroxyflavon). Insignificant cytotoxicity was noticed in SW 480, HeLa, MCF 10A when compared to MCF-7 cell lines exposed to different concentrations of purified quercetin for 24- 48 h. Similarly, the apoptosis by nuclei staining using Hoechst 33258 revealed a concentration dependent effect on MCF 7 cells only. This was further substantiated by caspase-9 and 3 induction and mitochondrial depolarization as revealed by flow cytometry. Overall, the results showed that quercetin and its derivatives induced effective apoptosis on MCF-7 cells. Quercetin isolated from the in vitro cell suspension culture of C. pulcherrima showed significant cytotoxicity and apoptotic activity towards MCF-7 cell lines as compared to other cell lines. Keywords:  Caesalpinia pulcherrima; quercetins; suspension culture; cytotoxicity; apoptotic.

scholarly journals In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae)

2009 ◽  
Vol 23 (1) ◽  
pp. 274-281 ◽  
Author(s):  
Tiago Santana Balbuena ◽  
Claudete Santa-Catarina ◽  
Vanildo Silveira ◽  
Massuo Jorge Kato ◽  
Eny Iochevet Segal Floh
Keyword(s):  
Plant Growth ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Activated Charcoal ◽  
Leaf Explants ◽  
Biologically Active ◽  
In Vitro Morphogenesis ◽  
Fresh Matter

Piper solmsianum is a shrub from Southeast Brazil in which many biologically active compounds were identified. The aim of this work was to establish a cell suspension culture system for this species. With this in mind, petiole and leaf explants obtained from in vitro plantlets were cultured in the presence of different plant growth regulator combinations (IAA, NAA, 2,4-D and BA). Root and indirect shoot adventitious formation, detected by histological analysis, was observed. Besides the different combinations of plant growth regulators, light regime and the supplement of activated charcoal (1.5 mg.l-1) were tested for callus induction and growth. Cultures maintained in light, on a 0.2 mg.l-1 2,4-D and 2 mg.l-1 BA supplemented medium, and in the absence of activated charcoal, showed the highest calli fresh matter increment. From a callus culture, cell suspension cultures were established and their growth and metabolite accumulation studied. The achieved results may be useful for further characterization of the activated secondary metabolites pathways in in vitro systems of P. solmsianum.

scholarly journals Elicitation of Bacoside Content Using Plant Growth Regulators in Cell Suspension Culture of Bacopa monnieri (L.) Wettst.

2018 ◽  
Vol 28 (2) ◽  
pp. 191-199 ◽  
Author(s):  
AV Kharde ◽  
SV Kore ◽  
MB Khetmalas
Keyword(s):  
Plant Growth ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Cell Suspension Culture ◽  
Casein Hydrolysate ◽  
Leaf Explants ◽  
Bacopa Monnieri ◽  
Naphthalene Acetic Acid

Cell suspension culture is used for the optimization of plant growth regulators used for the elicitation of bacoside content of Bacopa monnieri (L.) Wettst. Callus induction was carried out on MS supplemented with 2, 4-D (0.5 mg/l) by using leaf explants. For suspension culture MS liquid medium was supplemented with constant α-naphthalene acetic acid (0.5 mg/l) and casein hydrolysate (1 g/l) as well as the varied concentrations of 6-benzyleadenine, kinetin, thidiazuaron and chitosan. It was observed that bacoside production was increased by 6- benzyleadenine (0.5 mg/l), Kn (0.5 mg/l) and thidiazuaron (0.25 mg/l) was 3.07, 3.79 and 3.97 mg/g, respectively. This indicates that lower concentration of growth regulator favor the bacoside enhancement under in vitro conditions, while chitosan affects the bacoside production in suspension culture. In this study thidiazuaron at 0.25 mg/l induces maximum elicitation of bacoside content i.e. 3.97 mg/g. Plant Tissue Cult. & Biotech. 28(2): 191-199, 2018 (December)

Establishment of callus and cell suspension culture of Sophora alopecuroides Linn. for the production of oxymatrine

2020 ◽  
pp. 35-39
Author(s):  
M. Tsolmon ◽  
G Oyundari ◽  
Oyunbileg Yu ◽  
Kalaiselvi Senthil
Keyword(s):  
Nitric Oxide ◽  
Jasmonic Acid ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Callus Induction ◽  
Leaf Explants ◽  
Biologically Active ◽  
Induction Medium ◽  
Sophora Alopecuroides

Oxymatrine is one of the most important biologically active compounds and is present in Sophora alopecuroides L. The present investigation focuses on the development of an efficient tissue culture method to induce callus and cell suspension culture of S. alopecuroides by studying the effect of jasmonic acid and nitric oxide on cell suspension culture. Callus induction efficiency is high in axenic leaf explants grown in MS medium supplemented with 1.0 mg/L Kinetin (Kin), 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D). The cell suspension culture was developed using the same callus induction medium without agar. The maximum cell number and dry weight of suspension culture were obtained by the 9th day of incubation. The synthesis of oxymatrine is higher in jasmonic acid and nitric oxide (200 µMJA and 50 µMNO) combination (11.91 µg/g) when compared to the non-elicited control (8.3 µg/g) of callus.

Extraction, Identification And Determination of Antiviral And Anticancer Flavonoids By HPLC-DAD In Cell Suspension Culture of Melia Azedarach L.

2021 ◽  
Author(s):  
Fatemeh Ahmadpoor ◽  
Nasser Zare ◽  
Rasool Asghari-Zakaria ◽  
Parisa Sheikhzadeh-Mosadeg
Keyword(s):  
Secondary Metabolites ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Cultures ◽  
Cell Suspension Culture ◽  
Callus Induction ◽  
Leaf Explants ◽  
Melia Azedarach ◽  
Explant Type ◽  
Wide Range

Abstract Melia azedarach L. from the Meliaceae, contains a wide range of secondary metabolites used as antibacterial, antiviral, antioxidant and anticancer in traditional medicinal. In the present study, the effect of PGRs and explant type on callus induction and cell suspension culture establishment and growth were investigated. We investigated the biochemical properties, and production and accumulation of secondary metabolites such as rutin, quercetin and kaempferol in M. azedarach L. cell cultures. The results showed that the inflorescence and petiole explants had a high percentage of callus induction compared to the leaf explants, but the highest callus growth were observed in leaf explants in MS+3 mg/L NAA+5 mg/L BAP/3 mg/L Kin and 5 mg/L 2,4-D+5 mg/L Kin (6.307, 5.152 and 3.977 g/explant, respectively). The establishment and growth of cell cultures were significantly influenced by PGRs combination and explant type. The highest cell growth were obtained from the leaf and inflorescence callus transferred into the liquid MS medium supplemented with 1 mg/L 2,4-D+1 mg/L Kin (8.489 g/50 mL suspension) and 1 mg/L 2,4-D+1 mg/L BAP (6.852 g/50 mL suspension), respectively. HPLC analysis showed that the cell cultures derived from inflorescence callus in MS+3 mg/L NAA+1 mg/L BAP showed the highest of rutin (47.536 mg/g FW). However, the highest amount of quercetin (8.570 mg/g FW) and kaempferol (5.420 mg/g FW) were obtained from the petiole cell cultures in the MS+1 mg/L 2,4-D+1 mg/L Kin.

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