In vitro Plant Regeneration from Nodal Explants of Coleus forskohlii Briq. - An Important Medicinal Plant

2020 ◽  
Vol 30 (1) ◽  
pp. 143-148
Author(s):  
B Janarthanam ◽  
E Sumathi
Keyword(s):  
Basal Medium ◽  
Growth Conditions ◽  
Nodal Explants ◽  
Ex Situ ◽  
Direct Organogenesis ◽  
Coleus Forskohlii ◽  
Mass Propagation ◽  
Best Response ◽  
Ex Vitro Growth

An efficient in vitro mass propagation and promising protocol has been successfully standardized and developed for Coleus forskohlii through direct organogenesis from nodal explants. Nodal explants cultured onto MS basal medium supplemented with 4.44 μM BAP recorded the highest response and produced 24.3 ± 0.2 shoots per explant with an average shoot length 5.6 ± 0.4 cm after 30 days of culture. The in vitro shoots recorded higher response for development of rooting on half strength MS fortified with 2.46 μM IBA which produced the best response 7.8 ± 0.6 roots per in vitro shoot with an average root length of 4.3 ± 0.1 cm after 25 days. The in vitro rooted plantlets were transferred for hardening and 90% of the plantlets survived were successfully acclimatized and established in small plastic pots. This protocol recorded to be a highly repeatable, successful and rapid technique that could be utilized for the commercial mass propagation and ex situ conservation of Coleus forskohlii. It is important to note that the morphology of the in vitro plantlets of Coleus forskohlii showed a true-to-type growth habit, both in vitro and when transferred to ex vitro growth conditions. Plant Tissue Cult. & Biotech. 30(1): 143-148, 2020 (June)

scholarly journals In vitro mass propagation of Plumbago zeylanica L., through direct organogenesis

2012 ◽  
Vol 47 (3) ◽  
pp. 297-302
Author(s):  
AKMS Hassan ◽  
F Haque ◽  
MAA Jahan ◽  
SK Roy
Keyword(s):  
Room Temperature ◽  
Basal Medium ◽  
Nodal Explants ◽  
Direct Organogenesis ◽  
Shoot Induction ◽  
Mass Propagation ◽  
Plumbago Zeylanica ◽  
Half Strength ◽  
Regenerated Plantlets

An efficient protocol was developed for in vitro mass propagation of an important medicinal shrub, Plumbago zeylanica L., (Plumbaginaceae) through direct organogenesis using shoot tip and nodal explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP, in which 86.4% of nodal explants responded to produce maximum number (12.4 ± 0.66) of shoots per culture. In vitro raised shoots rooted on half strength MS medium with 0.5 mg/l IAA. For acclimatization and transplantation, the plantlets in the rooted culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%. DOI: http://dx.doi.org/10.3329/bjsir.v47i3.13063 Bangladesh J. Sci. Ind. Res. 47(3), 297-302 2012

scholarly journals The Influence of Explant Types and Orientation on in Vitro Culture

2011 ◽  
Vol 3 (3) ◽  
pp. 89-92
Author(s):  
Kamnoon KANCHANAPOOM ◽  
Nararatn PROMSORN
Keyword(s):  
Growth Regulators ◽  
Basal Medium ◽  
Ms Medium ◽  
Mass Propagation ◽  
Abaxial Surface ◽  
Musa Balbisiana ◽  
Lateral Buds ◽  
Best Response ◽  
Lying Down

Inflorescence, apical and lateral buds of Musa balbisiana ‘Kluai Hin’ (BBB group) were used to culture on MS medium supplemented with 22 μM BA and 15% (v/v) coconut water. Comparison of bud orientation showed that the best response of in vitro shoot tip proliferation was obtained with abaxial surface of buds lying down i.e. one side touching the medium (tilt). Mass propagation of shoot tips was obtained when cultured buds on MS medium containing 44 μM BA. Rooting was achieved by transferring the isolated shoots to MS basal medium without growth regulators. Rooted plantlets were acclimatized and successfully established in soil.

scholarly journals In Vitro Mass Propagation of Mimosa pudica L., Using Shoot Tip and Nodal Explants

1970 ◽  
Vol 45 (2) ◽  
pp. 95-100 ◽  
Author(s):  
AKM Sayeed Hassan ◽  
Rebeka Sultana ◽  
Miskat Ara Akhter Jahan ◽  
Rahima Khatun
Keyword(s):  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Shoot Tip ◽  
Nodal Explants ◽  
Mimosa Pudica ◽  
Mass Propagation ◽  
Half Strength ◽  
Proliferation In Vitro

An efficient protocol was established for in vitro mass propagation of a valuable medicinal shrubby plant, Mimosa pudica Linn., from shoot tip and nodal explants. Optimum in vitro shoot induction was observed from nodal explants on MS basal medium supplemented with 1.5 mg/l BAP + 0.5 mg/l NAA, in which 88.2% of the explants produced 9 shoots per culture within 3-4 weeks. Repeated subcultures in the same medium, resulted rapid shoot multiplication with 20.4 ± 1.20 shoots per culture within 12 weeks. The healthy in vitro raised shoots rooted on half strength MS medium with 0.5 mg/l IBA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 80%. Key words: Mimosa pudica; Medicinal plant; Shoot proliferation; In vitro mass propagation; Acclimatization DOI: 10.3329/bjsir.v45i2.5704Bangladesh J. Sci. Ind. Res. 45(2), 95-100, 2010

scholarly journals In vitro shoot multiplication of the biodiesel plant Jatropha curcas L. through direct organogenesis

2014 ◽  
Vol 49 (1) ◽  
pp. 41-46 ◽  
Author(s):  
MAKMS Hassan ◽  
N Begum ◽  
LS Bari ◽  
MAA Jahan
Keyword(s):  
Jatropha Curcas ◽  
Basal Medium ◽  
Shoot Multiplication ◽  
Nodal Explants ◽  
Direct Organogenesis ◽  
Shoot Induction ◽  
Jatropha Curcas L ◽  
Half Strength ◽  
Regenerated Plantlets

An efficient protocol was established for in vitro shoot multiplication of the biodiesel plant, Jatropha curcas L. (Euphorbiaceae) through direct organogenesis using shoot tip and nodal explants. Best shoot induction was observed on MS basal medium supplemented with 1.5 mg/l BAP + 0.5 mg/l NAA, in which 86.2% of nodal explants responded to produce maximum number (7.2 ± 0.68) of shoots per culture. In vitro raised shoots rooted on half strength MS medium with 1.0 mg/l IAA. The survival rate of regenerated plantlets was 85%. DOI: http://dx.doi.org/10.3329/bjsir.v49i1.18854 Bangladesh J. Sci. Ind. Res. 49(1), 41-46, 2014

scholarly journals In vitro plant regeneration of Coccinea cordifolia (Linn.) Cogn., an anti-diabetic medicinal plant

2012 ◽  
Vol 47 (2) ◽  
pp. 187-190
Author(s):  
Chapol Kumar Roy ◽  
John Liton Munshia ◽  
Nadira Begum ◽  
Rahima Khatun ◽  
AKM Sayeed Hassanb
Keyword(s):  
Plant Regeneration ◽  
Medicinal Plant ◽  
Basal Medium ◽  
Nodal Explants ◽  
Direct Organogenesis ◽  
In Vitro Plant Regeneration ◽  
Half Strength ◽  
In Vitro Plant ◽  
Regenerated Plantlets

An efficient protocol was developed for in vitro plant regeneration of a popularly used anti-diabetic medicinal plant, Coccinea cordifolia (Linn.) Cogn.(Cucurbitaceae) through direct organogenesis using shoot tip and nodal explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP, in which 88.2% of nodal explants responded to produce maximum number (6.2 ± 0.58) of shoots per culture. In vitro raised shoots rooted on half strength MS medium with 0.5 mg/l IBA. The survival rate of regenerated plantlets was 85%.   DOI: http://dx.doi.org/10.3329/bjsir.v47i2.11450   Bangladesh J. Sci. Ind. Res. 47(2), 187-190, 2012  

scholarly journals In vitro organogenesis and plant regeneration of Passiflora xishuangbannaensis, a species with extremely small populations

2021 ◽  
Author(s):  
Yuan-yuan Meng ◽  
Shi-jie Song ◽  
Sven Landrein
Keyword(s):  
Plant Regeneration ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Basal Medium ◽  
Ex Situ ◽  
Naphthaleneacetic Acid ◽  
South American ◽  
South American Species

Abstract Passiflora xishuangbannaensis (Passifloraceae) is endemic to a few sites of Mengyang nature reserve in Yunnan, Xishuangbanna and less than 40 individuals have been recorded. Nine Passiflora species are endemic to Yunnan with most species occurring in South America, making P. xishuangbannaensis highly significant and emblematic to the conservation work in the region. This study is designed to provide the first protocol for in vitro organogenesis and plant regeneration for ex situ conservation and reintroduction for an Asian Passiflora species. Using internodes, petioles and tendrils we optimize calli formation and root elongation using several plant growth regulators, individually or in combination. We also assess the genetic stability of regenerated cells. The maximum callus induction and shoot bud differentiation were both achieved on half Murashige and Skoog basal medium supplemented with 4.44 µM 6-Benzylaminopurine and 1.08 µM 1-Naphthaleneacetic acid. The best rooting was achieved from 30 days old, regenerated shoots on half Murashige and Skoog basal medium supplemented with 1.08 µM 1-Naphthaleneacetic acid. Micropropagated plants were subjected to inter simple sequence repeat markers analyses. Collectively, 86 bands were generated from 6 primers of which 12 bands were polymorphic, showing genetic variation between the regenerated plantlets and the original plant. Response to plant growth regulators was more specific than most other studies using South American species, which could be explained by the morphological and physiological differences between South American and Asian Passiflora species

scholarly journals In vitro spore germination and early gametophyte development of Cibotium barometz (L.) J. Sm. in different media

2020 ◽  
Vol 21 (11) ◽  
Author(s):  
Yupi ISNAINI ◽  
Titien Ngatinem Praptosuwiryo
Keyword(s):  
Spore Germination ◽  
Culture Media ◽  
Basal Medium ◽  
Modern Medicine ◽  
Ex Situ ◽  
Naphthalene Acetic Acid ◽  
Gametophyte Development ◽  
In The Wild ◽  
Rare And Endangered

Abstract. Isnaini Y, Praptosuwiryo TNg. 2020. In vitro spore germination and early gametophyte development of Cibotium barometz (L.) J. Sm. in different media. Biodiversitas 21: 5373-5381. Cibotium barometz (L.) J. Sm. is known as the golden chicken fern and included in Appendix II of CITES. It is an important export commodity for traditional and modern medicine. Globally, populations of this species are under significant pressure due to overexploitation in the wild. In vitro culture is one of the technologies used for ex-situ propagation and conservation of rare and endangered ferns and lycophytes. This study’s objectives were: (i) to observe in vitro spore germination and early gametophyte development of C. barometz, and (ii) to determine the best culture medium for rapid spore germination and early development of the gametophytes. The sterilized spores were sown in half-strength Murashige & Skoog (½MS) basal medium supplemented with combinations of 6-Benzylaminopurine (BAP) and α-Naphthalene acetic acid (NAA). A factorial combination of four BAP concentrations (0, 2, 4, and 6 mg L-1) with four concentrations of NAA (0; 0.01; 0.03 and 0.05 mg L-1) created 16 treatments replicated in a Completely Randomized Design. Spore germination of C. barometz was observed to be Vittaria-type, and its prothallial development was Drynaria-type. Spore germination started 7-14 days after sowing. Young heart-shape gametophytes consisting of 110-240 cells were formed in 45-61 days after sowing. The two best spore culture media for rapid spore germination and development of C. barometz gametophytes were ½ MS with or without 2 mg L-1 BAP.

scholarly journals Mass Propagation of Nauclea diderrichii (de Willd. & Th. Dur) Merr. Seedlings through Tissue Culture Technique

2021 ◽  
Vol 31 (1) ◽  
pp. 51-60
Author(s):  
RI Oyediran ◽  
JO Afolabi ◽  
DB Olomola ◽  
FO Akanni
Keyword(s):  
Tissue Culture ◽  
Adventitious Shoots ◽  
Basal Medium ◽  
Culture Technique ◽  
Seedling Production ◽  
Mass Propagation ◽  
In Vitro Plantlets ◽  
Number Of Leaves ◽  
Nauclea Diderrichii

Nauclea diderrichii is a tree species of economic importance. However, its plantation establishment is limited by inadequate seedling production. Hence, there is ample scope of tissue culture for its mass propagation. Its in vitro plantlets development as affected by media strengths indicated that 100 % seed germination was obtained in full MS basal medium while the least (3.35 %) was from quarter-strength at 8 Weeks after inoculation (WAI). The effects of BAP and NAA assessed on the growth of its sub-cultured plantlets showed that highest number of leaves (17) and adventitious shoots (3) were obtained from MS basal medium supplemented with 0.1 mg/l BAP only. Whereas, highest shoot length (3.61 cm) and average number of roots (5/plantlet) were obtained from the same medium without hormone(s) at 8 WAI. Further sub-culturing into MS with 0.05 mg/l NAA resulted into plantlets having optimum shoot and massive root growth ready for acclimatization in 6 WAI. The plantlets were successfully acclimatized using coconuthusk/ topsoil mixture with 90 % survival. Plant Tissue Cult. & Biotech. 31(1): 51-60, 2021 (June)

scholarly journals Mass Propagation of Feronia limonia L. through Tissue Culture

1970 ◽  
Vol 45 (1) ◽  
pp. 75-78 ◽  
Author(s):  
Shahina Islam ◽  
Mosfequa Zahan ◽  
Shahina Akter ◽  
Tanjina Akhtar Banu ◽  
Ahashan Habib ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Key Words ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Nodal Explants ◽  
Ms Medium ◽  
Ex Vitro ◽  
Mass Propagation

An efficient mass propagation method for Feronia limonia was developed from excised shoot tips and nodal explants of in vitro grown seedlings. Explants were cultured on MS medium with different conc. of NAA, Kn, IAA and BAP singly or in combinations. Highest number of micro shoots and better plant growth were obtained from these two explants on MS medium supplemented with 0.2 mg/l BAP alone. The regenerated shoots were successfully rooted on MS medium supplemented with 0.5 mg/l NAA. The in vitro raised plantlets were successfully established in soil following the formation of roots with 100% survivability under ex vitro condition. Key words: Feronia limonia; Mass propagation; Node; Shoot tips; Multiple shoot DOI: 10.3329/bjsir.v45i1.5186 Bangladesh J. Sci. Ind. Res. 45(1), 75-78, 2010

In vitro mass propagation of Piper betle L

2019 ◽  
Vol 48 (3) ◽  
pp. 559-566
Author(s):  
Salim Khan ◽  
Barna Goswami ◽  
Shahina Akter ◽  
Mousona Islam ◽  
Afsana Huq Noon ◽  
...  
Keyword(s):  
Nodal Explants ◽  
Direct Regeneration ◽  
Leaf Segment ◽  
Piper Betle ◽  
Nodal Segment ◽  
Root Induction ◽  
Regeneration System ◽  
Petiole Explants ◽  
Best Response

An efficient in vitro regeneration system was developed for Piper betle L. through direct and indirect organogenesis from nodal segment, leaf segment and petiole explants. Highest direct regeneration was recorded when nodal explants were cultured on MS with 1.0 mg/l BAP and 1.0 mg/l Kn where 80% explants produced multiple shoots and the average number of shoots per explants were 3.20. Remarkable results on callus induction and shoot initiation were observed when the explants cultured on MS + 2.0 mg/l BAP + 0.5 mg/l Kn + 1.0 mg/l IAA. It was observed that nodal explants were showed best response on shoot/explants 13.2 ± 4.5 after 8 weeks of callus culture on MS medium with 0.5 mg/l BAP. The best response towards root induction was observed on half strength of MS with 0.25 mg/l IBA. The well rooted plants were successfully acclimatized and transferred to soil.

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