scholarly journals Simultaneous Spectrophotometric Estimation and Validation of Domperidone, Tramadol Hydrochloride and Acetaminophen in Tablet Dosage Form

2011 ◽  
Vol 3 (1) ◽  
pp. 28-33
Author(s):  
Vikas Jain ◽  
Rajesh Sharma
Keyword(s):  
Component Analysis ◽  
Solvent Mixture ◽  
Simultaneous Equation ◽  
Pharmaceutical Products ◽  
Equation Method ◽  
Simultaneous Estimation ◽  
Tramadol Hydrochloride ◽  
Spectrophotometric Methods ◽  
Ich Guidelines ◽  
Tablet Dosage

Simultaneous estimation of active ingredients in multi-component pharmaceutical products normally requires the use of separation techniques, such as HPLC, HPTLC or GC, followed by their quantitation. Presented here are two spectrophotometric methods that do not require prior separation for simultaneous estimation of three drugs; acetaminophen, tramadol hydrochloride and domperidone in a tablet formulation. Shimadzu UV 1700 capable of multi-component analysis was used for quantitation. Method A is based on the simultaneous equation and method B on the multi-component analysis. The absorption maxima of the drugs found to be at 244nm, 271.5nm and 284.5nm respectively for acetaminophen, tramadol hydrochloride and domperidone in methanol/0.1 N HCl (1:2) solvent mixture. Acetaminophen, tramadol hydrochloride and domperidone obeyed Beer's law in the concentration range of 2-22 μg/ml, 10-55 μg/ml and 30-300 μg/ml respectively. The simultaneous equation method is based on the additivity of absorbances and multi-component analysis involves recording of absorbances of standard solutions at 244nm, 250nm, 271.5nm and 284.5nm. These were processed by means of statistical calculations and results of sample solution were obtained. Result of analysis for both methods were tested and validated for various parameters according to ICH guidelines. Key Words: simultaneous equation method; multicomponent analysis; acetaminophen; tramadol hydrochloride; domperidone. DOI: 10.3329/sjps.v3i1.2655S. J. Pharm. Sci. 3(1): 28-33

NEW SENSITIVE UV SPECTROPHOTOMETRIC METHODS FOR SIMULTANEOUS ESTIMATION OF ZIDOVUDINE AND LAMIVUDINE IN FIXED DOSE COMBINATION

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (03) ◽  
pp. 28-33
Author(s):  
A Chauhan ◽  
◽  
P.K Arora ◽  
R Nagpal ◽  
D Duggal ◽  
...  
Keyword(s):  
Wave Length ◽  
Estimation Method ◽  
Simultaneous Equation ◽  
Fixed Dose Combination ◽  
Fixed Dose ◽  
Simultaneous Estimation ◽  
Spectrophotometric Methods ◽  
Ich Guidelines ◽  
Dose Combination ◽  
Tablet Dosage

Two new, simple, accurate and sensitive UV spectrophotometric methods have been developed and subsequently validated for the simultaneous estimation of zidovudine and lamivudine in a fixed dose combination. Zidovudine and lamivudine have an absorption maxima at 267.3 nm and 272.3 nm respectively. The first method is based upon the simultaneous equation and second upon the determination of Q value. The simultaneous estimation method is based upon the measurements of ratios of absorptivity and absorbance, of both the components at their absorption maxima. The method of Q analysis is based on the measurement of ratios of absorptivity and absorbance, of both components at two selected wavelengths; one is an isoabsorptive point i.e. 270.1 nm and other being the wave length maxima of any of the two components, say λmax of zidovudine i.e. 267.3 nm. Both zidovudine and lamivudine shows linearity over the concentration range of 4-12 ppm at their respective absorption maxima and at isoabsorptive point. The assay and recovery studies from fixed dose combination as tablet dosage form are indicative of accuracy of the proposed methods. The developed methods were validated in accordance to International Conference on Harmonization (ICH) guidelines for linearity, range, accuracy and precision.

scholarly journals Simultaneous Spectrophotometric Method for Determination of Emtricitabine and Tenofovir Disoproxil Fumarate in Three-Component Tablet Formulation Containing Rilpivirine Hydrochloride

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
S. Venkatesan ◽  
N. Kannappan
Keyword(s):  
Spectrophotometric Method ◽  
Analytical Method ◽  
Tenofovir Disoproxil Fumarate ◽  
Dosage Form ◽  
Simultaneous Equation ◽  
Equation Method ◽  
Simultaneous Estimation ◽  
Ich Guidelines ◽  
Tablet Dosage

Developing a single analytical method for estimation of individual drug from a multidrug composition is a very challenging task. A complexation, derivatization, extraction, evaporation, and sensitive-free direct UV spectrophotometric method is developed and validated for the simultaneous estimation of some antiviral drugs such as emtricitabine (EMT), tenofovir disoproxil fumarate (TDF), and rilpivirine HCl (RPV) in tablet dosage form by Vierordt’s method. The solutions of standard and sample were prepared in methanol. The λmax⁡ for emtricitabine, tenofovir disoproxil fumarate, and rilpivirine hydrochloride were 240.8 nm, 257.6 nm, and 305.6 nm, respectively. Calibration curves are linear in the concentration ranges 4–12 μg/ml for EMT, 6–18 μg/ml for TDF, and 0.5–1.5 μg/ml for RPV, respectively. Results of analysis of simultaneous equation method were analyzed and validated for various parameters according to ICH guidelines.

scholarly journals Simultaneous Spectrophotometric Estimation of Telmisartan and Amlodipine Besylate in Tablet Dosage Form

2015 ◽  
Vol 3 (03) ◽  
pp. 50-54 ◽  
Author(s):  
N. K. Gupta ◽  
A. Peepliwal ◽  
D. S. Rathore ◽  
P. Gupta
Keyword(s):  
Quality Control ◽  
Dosage Form ◽  
Simultaneous Equation ◽  
Dosage Forms ◽  
Simultaneous Estimation ◽  
Amlodipine Besylate ◽  
Spectrophotometric Methods ◽  
Ich Guidelines ◽  
Tablet Dosage ◽  
Routine Quality Control

A simple, accurate and reproducible spectrophotometric methods have been developed for the simultaneous estimation of Telmisartan (TEL) and Amlodipine Besylate (AML) in combined tablet dosage forms. The method involves determination using the simultaneous equation method, the sampling wavelengths selected are ‘TLM’ = 297nm.and ‘AML’ =238nm., over the concentration ranges of 8-48μg/ml for ‘TEL’ and 1-6 μg/ml for ‘AML’ respectively. The method was validated for linearity, accuracy, precision, robustness and application for assay as per ICH guidelines. The proposed method is simple, economical, accurate and precise, and could be successfully employed in routine quality control for the simultaneous analysis of Telmisartan (TEL) and Amlodipine Besylate (AML).

scholarly journals SIMULTANEOUS UV SPECTROPHOTOMETRIC METHODS FOR ESTIMATION OF CEFADROXIL AND PROBENECID IN TABLET DOSAGE FORM

2018 ◽  
Vol 1 (3) ◽  
pp. 19-23
Author(s):  
Mayur S. Jain ◽  
Sunil R. Bavaskar ◽  
Shashikant D. Barhate ◽  
Jintendra D. Fegade
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Simultaneous Equation ◽  
Equation Method ◽  
Ratio Method ◽  
Simultaneous Estimation ◽  
Isobestic Point ◽  
Spectrophotometric Methods ◽  
Absorption Ratio ◽  
Tablet Dosage

Two methods for simultaneous estimation of Cefadroxil and Probenecid in combined tablet dosage form have been developed. The first UV spectrophotometric method was a determination using the simultaneous equation method at 233 nm and 247 nm. The second UV spectrophotometric method is the Q – analysis (absorption ratio) method, which involves the formation of absorbance equation at 242 nm (Isobestic point) and at 247 nm the maximum absorption of Probenecid . The linearity ranges for Cefadroxil and Probenecid both were 10-60μg/ml respectively. The accuracy of the methods was assessed by recovery studies was found to be 99.43±0.75 and 99.69±0.40 for simultaneous equation method and 99.23±0.34 and 99.56±0.16 for absorption ratio method for Cefadroxil and Probenecid respectively. These methods are simple, accurate and rapid; those require no preliminary separation and can therefore be used for routine analysis of both drugs in quality control laboratories.

scholarly journals Simultaneous Estimation of Domperidone and Pantoprazole in Solid Dosage Form by UV Spectrophotometry

2006 ◽  
Vol 3 (3) ◽  
pp. 142-145
Author(s):  
P. Ravi Kumar ◽  
P. Bhanu Prakash ◽  
M. Murali Krishna ◽  
M. Santha Yadav ◽  
C. Asha Deepthi
Keyword(s):  
Simultaneous Equation ◽  
Simultaneous Equations ◽  
Simultaneous Estimation ◽  
Uv Spectrophotometry ◽  
Q Value ◽  
Value Analysis ◽  
Spectrophotometric Methods ◽  
Solid Dosage ◽  
Tablet Dosage

Domperidone is an antiemetic and pantoprazole is an antiulcer drug. Simple, precise, rapid and selective simultaneous equation and Q- analysis UV spectrophotometric methods have been developed for the simultaneous determination of domperidone and pantoprazole from combined tablet dosage forms. The methods involve solving of simultaneous equations and Q-value analysis based on measurement absorptivity at 216, 287 and 290 nm respectively. Linearity lies between 1-15 mcg/mL for domperidone and 0-50 mcg/mL for pantoprazole.

scholarly journals Simultaneous Estimation of Rosuvastatin Calcium and Fenofibrate in Bulk and in Tablet Dosage Form by UV-Spectrophotometry and RP-HPLC

1970 ◽  
Vol 4 (1) ◽  
pp. 58-63 ◽  
Author(s):  
Anandakumar Karunakaran ◽  
Vetsa Subhash ◽  
Ramu Chinthala ◽  
Jayamaryapan Muthuvijayan
Keyword(s):  
Simultaneous Equation ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Uv Spectrophotometry ◽  
Hplc Separation ◽  
Rp Hplc ◽  
Ich Guidelines ◽  
Tablet Dosage ◽  
Rosuvastatin Calcium

Two methods are described for the simultaneous estimation of Rosuvastatin Calcium and Fenofibrate in binary mixture. The first method was based on UV Spectrophotometric determination of two drugs, using simultaneous equation method. It involves absorbance measurement at 243nm (λmax of Rosuvastatin Calcium) and 287nm (λmax of Fenofibrate) in methanol; linearity was obtained in the range of 1-6 μg/ml and 4-28 μg/ml for Rosuvastatin Calcium and Fenofibrate, respectively. The second method was based on HPLC separation of two drugs in reverse phase mode using Luna C18 column. Linearity was obtained in the concentration of 1-7 μg/ml and 4-28 μg/ml for Rosuvastatin Calcium and Fenofibrate, respectively. Both these methods have been successively applied to pharmaceutical formulation and were validated according to ICH guidelines.   Key words: Rosuvastatin Calcium; Fenofibrate; UV Spectrophotometry; HPLC; Method validation DOI: http://dx.doi.org/10.3329/sjps.v4i1.8868 SJPS 2011; 4(1): 58-63

scholarly journals UV Spectrophotometric Methods to Quantify Alogliptin Benzoate and Pioglitazone Hydrochloride

2021 ◽  
pp. 31-41
Author(s):  
Dhanya B. Sen ◽  
Ashim K. Sen ◽  
Aarti S. Zanwar ◽  
Harshita Pandey ◽  
Rajesh A. Maheshwari
Keyword(s):  
Limit Of Detection ◽  
Spectroscopic Method ◽  
Simultaneous Estimation ◽  
Control Analysis ◽  
Limit Of Quantification ◽  
Spectrophotometric Methods ◽  
First Derivative ◽  
Ich Guidelines ◽  
The Difference ◽  
Tablet Dosage

Three new, precise, accurate and sensitive UV-Spectrophotometric methods namely Ratio Difference Spectroscopic Method (RDSM), First Derivative of Ratio Spectra Method (DR1) and Area Under Curve Method (AUC) were developed and validated for simultaneous assessment of alogliptin benzoate (ALO) and pioglitazone hydrochloride (PIO) in tablet dosage form. In RDSM, ratio spectra of both the drugs were recorded by dividing the mixtures using interfering drug as divisor. Then the difference between the amplitudes of obtained ratio spectra was measured at 288 and 291 nm for ALO and 236 and 245 nm for PIO. The second method DR1, where the first derivative of ratio spectra of both the drugs were recorded and the first derivative signal was measured at 290 nm for ALO and 276.8 nm for PIO. The scaling factor was fixed as 1 and wavelength interval (Δλ) as 2 for recording the first derivative of ratio spectra.  In the third method (AUC), peak area of recorded zero order spectra was measured at 276 ± 10 nm for ALO and 267.8 ± 10 nm for PIO. All three proposed methods were validated according to “International Conference on Harmonization” (ICH) guidelines parameters. For all three methods, ALO and PIO obeyed Beer’s law in the range of 0.5-5 & 1.8-18 µg/ml, respectively. The % RSD of repeatability of measurement, intra-day and inter-day precision were found to be less than 2 for all three methods. Limit of detection (LOD) and Limit of quantification (LOQ) of the drugs were calculated which proved the sensitivity of the methods. The accuracy ranged between 98-101% for all three methods. No interference from pharmaceutical excipients present in the formulation was observed.  These proposed methods were found to be simple, sensitive, accurate and precise and can be applied to the simultaneous estimation of ALO and PIO in combined tablet formulation and also appropriate for routine quality control analysis.

scholarly journals Development and Validation of Spectrophotometric Methods for Simultaneous Estimation of Valsartan and Hydrochlorothiazide in Tablet Dosage Form

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Monika L. Jadhav ◽  
Manoj V. Girase ◽  
Shripad K. Tidme ◽  
Manish S. Junagade
Keyword(s):  
Dosage Form ◽  
Pharmaceutical Formulations ◽  
Simultaneous Equation ◽  
Ratio Method ◽  
Simultaneous Estimation ◽  
Spectrophotometric Methods ◽  
Absorbance Ratio ◽  
Standard Additions ◽  
Development And Validation ◽  
Tablet Dosage

Two UV-spectrophotometric methods have been developed and validated for simultaneous estimation of valsartan and hydrochlorothiazide in a tablet dosage form. The first method employed solving of simultaneous equations based on the measurement of absorbance at two wavelengths, 249.4 nm and 272.6 nm, λmax for valsartan and hydrochlorothiazide, respectively. The second method was absorbance ratio method, which involves formation of Q-absorbance equation at 258.4 nm (isoabsorptive point) and also at 272.6 nm (λmax of hydrochlorothiazide). The methods were found to be linear between the range of 5–30 µg/mL for valsartan and 4–24 μg/mL for hydrochlorothiazide using 0.1 N NaOH as solvent. The mean percentage recovery was found to be 100.20% and 100.19% for the simultaneous equation method and 98.56% and 97.96% for the absorbance ratio method, for valsartan and hydrochlorothiazide, respectively, at three different levels of standard additions. The precision (intraday, interday) of methods was found within limits (RSD<2%). It could be concluded from the results obtained in the present investigation that the two methods for simultaneous estimation of valsartan and hydrochlorothiazide in tablet dosage form are simple, rapid, accurate, precise and economical and can be used, successfully, in the quality control of pharmaceutical formulations and other routine laboratory analysis.

scholarly journals New Simple Spectrophotometric Method for the Simultaneous Estimation of Paracetamol and Flupirtine Maleate in Pure and Pharmaceutical Dosage Form

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
P. Giriraj ◽  
T. Sivakkumar
Keyword(s):  
Correlation Coefficient ◽  
Spectrophotometric Method ◽  
Dosage Form ◽  
Simultaneous Equation ◽  
Equation Method ◽  
Simultaneous Estimation ◽  
Pharmaceutical Dosage Form ◽  
Precision Study ◽  
Tablet Dosage ◽  
Concentration Levels

A new, simple, precise, accurate, reproducible, and efficient Vierordt’s method or simultaneous equation method was developed and validated for simultaneous estimation of paracetamol and flupirtine maleate in pure and pharmaceutical dosage form. The method was based on the measurement of absorbance at two wavelengths 245 nm and 344.5 nm, λmax of paracetamol and flupiritine maleate in 0.1 N HCl correspondingly. Calibration curves of paracetamol and flupiritine maleate were found to be linear in the concentration ranges of 5–15 μg/mL and 1.53–4.61 μg/mL, respectively, with their correlation coefficient values (R2) 0.999. LOD and LOQ were 185.90 ng/mL and 563.38 ng/mL for paracetamol and 78.89 ng/mL and 239.06 ng/mL for flupiritine maleate. In the precision study, the % RSD value was found within limits (RSD<2%). The percentage recovery at various concentration levels varied from 99.18 to 100.02% for paracetamol and 98.47 to 100.09% for flupiritine maleate confirming that the projected method is accurate. It could be concluded from the results obtained in the present investigation that this method for simultaneous estimation of paracetamol and flupirtine maleate in pure and tablet dosage form is simple, accurate, precise, and economical. The proposed method can be applied successfully for the simultaneous estimation of paracetamol and flupiritine maleate in pure and pharmaceutical dosage form.

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