scholarly journals Homozygotes for HbE and Anaemia -3 Years Study in Rajshahi

2011 ◽  
Vol 24 (1) ◽  
pp. 34-37
Author(s):  
MR Khan ◽  
MS Uddin Shah ◽  
MA Aziz ◽  
MMR Khan ◽  
M Khalilur Rahman ◽  
...  
Keyword(s):  
Complete Blood Count ◽  
Antenatal Diagnosis ◽  
Blood Film ◽  
Hb E ◽  
Peripheral Blood Film ◽  
Haemoglobin Disorders ◽  
Abnormal Hemoglobin ◽  
Hemoglobin Variants ◽  
Actual Prevalence ◽  
Comprehensive Study

HbE is the most common abnormal hemoglobin of Southeast Asia and is almost limited to this region. It is estimated that 30 million people are heterozygous for HbE and that 1 million are homozygous. Aim of this study was to know the number of homozygous for haemoglobin E condition (Hb E disease) patients among patients suspected of having hereditary haemoglobin disorders referred to the regional laboratory, in Rajshahi. From April 2009 to March 2012, anaemic patients suspected of having hereditary haemoglobin disorder (haemoglobinopathy or thalassemia) were referred to the divisional laboratory, Rajshahi for hemoglobin electrophoresis from the physicians of different areas of Rajshahi division and part of Khulna division. Patient’s age ranging from 01 to 85 yrs of both sexes. Two to three milliliter blood was collected in tubes containing EDTA for complete blood count (CBC) and analysis of hemoglobin variants. CBC was measured by the Erma PC 604 particle counter. Peripheral blood film were examined after staining with Wright’s stain.9 Hemoglobin electrophoresis was carried out on cellulose acetate using TEB buffer, pH 8.6.9. Of 707 anaemic patients suspected of having hereditary haemoglobin disorder (haemoglobinopathy or thalassemia) 333 (47%) were abnormal. Of this abnormal patients 35 (11%) revealed a thalassemia-like disorder, 53 ( 17%) patients revealed haemglobin E disease, 46 ( 14%) patients haemglobin E trait and 188 ( 57%) patients having double heterozygous (Hb E/β thalassemia) disorder. The birth incidence for homozygous babies and or Eβ thalassemia would be increases per year. Population groups with higher frequencies require screening programmes and facilities for antenatal diagnosis. Future comprehensive study are require to know the actual prevalence. TAJ 2011; 24(1): 34-37

scholarly journals Deranged hematological profile in patients presenting with malarial parasitaemia

2021 ◽  
Author(s):  
Amber Haroon ◽  
Huda Zameer ◽  
Aneeqa Naz ◽  
Sohaib Afzal ◽  
Tooba Ammar ◽  
...  
Keyword(s):  
Platelet Count ◽  
Plasmodium Vivax ◽  
Blood Count ◽  
Complete Blood Count ◽  
Hematological Parameters ◽  
Blood Film ◽  
Bleeding Disorders ◽  
Peripheral Blood Film ◽  
Duration Of Disease ◽  
Low Platelet Count

Background: Alterations in the hematological parameters are thought to have the capacity to act as an adjuvant tool in strengthening the suspicion of malaria, thereby prompting a more meticulous search for malaria parasites.Methods: 186 cases with malaria positive for immunochromatographic test (ICT) malaria presenting with fever ≥101 °F, confirmed on peripheral blood film, of 20-60 years were included. Patients with coagulopathy and bleeding disorders such as hemophilia, brain tumors, tuberculosis meningitis, viral or bacterial encephalitis and multiple sclerosis were excluded. Sample was taken to the laboratory, for routine investigations like ICT malaria and complete blood count (CBC) to diagnose anemia and low platelet count.Results: Patients of 20 to 60 years of age with mean 41.80±8.51. Out of 186 patients, 101 (54.30%) were male and 85 (45.70%) were female with 1:2:1 ratio. Mean duration of disease was 4.91±1.32 days. Frequency of derangement in hematological parameters was anemia in 27 (14.52%) patients and 142 (76.34%) with thrombocytopenia. 127 cases were of Plasmodium vivax and 59 cases were of Plasmodium falciparum. Patients were more anemic as disease period prolonged. Patients infected by Plasmodium vivax showed more tendencies towards anemia and reduction in platelet count as compare to Plasmodium falciparum.Conclusions: The frequency of derangement in hematological parameters i.e. anemia and low platelet count, among malarial patients is quite high and more cases were of Plasmodium vivax and it showed anemia and thrombocytopenia was more pronounced in Plasmodium vivax.

scholarly journals Haemoglobin D Trait-An Uncommon Haemoglobinopathy in Chattogram: A Case Report

2021 ◽  
Vol 20 (1) ◽  
pp. 81-83
Author(s):  
Razia Sultana ◽  
Tani Gosh
Keyword(s):  
Laboratory Medicine ◽  
Blood Film ◽  
Subsequent Pregnancy ◽  
Heterozygous State ◽  
Compound Heterozygosity ◽  
Medicine Department ◽  
Microcytic Hypochromic Anaemia ◽  
Hypochromic Anaemia ◽  
Hb E ◽  
Peripheral Blood Film

This case presents haemoglobin D Trait (Hb-D) which is a rare disease in Bangladesh. It is a heterozygous state for haemoglobin D who came to Laboratory Medicine Department of Chattogram Maa Shishu-O-General Hospital (CMSOGH) for heamoglobin electrophoresis. Our patient was 9 years old girl from Noakhali, Bangladesh. She was clinically silent with no splenomegaly. Her peripheral blood film was microcytic hypochromic anaemia and Capillary Electrophoresis (CE) of Hb revealed Hb D Trait. On family screening her father was Hb E trait, mother was Hb D trait and younger sister turned out normal Hb study. Compound heterozygosity for haemoglobin D and haemoglobin E may be present in subsequent pregnancy of her mother. Chatt Maa Shi Hosp Med Coll J; Vol.20 (1); January 2021; Page 81-83

scholarly journals A 65–year–old male with moderate anemia, low-grade fever and weight loss

2020 ◽  
Vol 13 (3) ◽  
pp. 97-110
Author(s):  
Mohammad Mizanur Rahman ◽  
Md. Monirul Islam ◽  
Mohammad Shahidul Islam
Keyword(s):  
Bone Marrow ◽  
Weight Loss ◽  
Complete Blood Count ◽  
Bone Marrow Aspiration ◽  
Armed Forces ◽  
Blood Film ◽  
Low Grade ◽  
Peripheral Blood Film ◽  
Marked Weight ◽  
Moderate Anemia

This article has no abstract. The first 100 words appear below: A 65-year-old male hailing from Lolua, Tangail north bordering district of Dhaka, Bangladesh, reported to the Department of Hematology, Armed Forces Institute of Pathology with the complaints of marked weight loss, generalized weakness, low-grade fever and anorexia. He was referred from Kurmitola General Hospital, Dhaka, Bangladesh for bone marrow aspiration and examination. Before aspirating the bone marrow, a detailed history was taken which revealed that he had been suffering from the above mentioned symptoms for the last 10 months. During this time, he reported to the local physicians who investigated with routine laboratory tests such as complete blood count, peripheral blood film

scholarly journals Hereditary Haemoglobin Disorders of Anaemic Patients Attending in a Rural Tertiary Level Hospital

KYAMC Journal ◽  
2017 ◽  
Vol 6 (1) ◽  
pp. 545-549
Author(s):  
Md Shahadat Hossain ◽  
Md Mahbur Rashid Sarker ◽  
Faisal Kabir
Keyword(s):  
Medical Treatment ◽  
Blood Film ◽  
Severe Illness ◽  
Tertiary Level ◽  
Microcytic Anaemia ◽  
Long Period ◽  
Number Of Patients ◽  
Peripheral Blood Film ◽  
Haemoglobin Disorders ◽  
Level Hospital

Background: Regularly patients attending in the hospital with severe illness for medical treatment. A large number of patients came with features of anaemia. Many of them show hereditary haemoglobin disorders.Objective: This study was done to find out the pattern of hereditary haemoglobin disorders of anaemic patients attending in the hospital for medical treatment in a tertiary level rural hospital.Method: This descriptive type of retrospective study was performed with 151 cases of anaemic patients whose whole blood show abnormality in haemolobin on capillary haemoglobin electrophoresis. Haemoglobin as well as haematocrit and red blood cells indices were performed by using fully automated haematology lab automation (Sysmex XN-1000). Then haemoglobin electrophoresis was performed to see haemoglobin disorder by using Capillary-2 Haemoglobin Electrophoresis, Sebia, France.Results: All of the 151 cases of hereditary haemoglobin disorders were categorized into four groups. Of those Haemoglobin-E ß thalassaemia was 64.90%, ß thalassaemia minor was 17.22%, Haemoglobin-E haemoglobinopathies was 11.92% and ß thalassaemia major was 5.96% of cases. Among 151 patients; 106 (70.20%) patients were severely anaemic, 30 (19.87%) patients were moderately anaemic and 15 (9.93%) patients were mildly anaemic. Among 98 patients of haemoglobin-E ß thalassaemia, 87 (88.76%) patients were severely anaemic.Conclusion: All anaemic patients especially who suffers from anaemia for a long period of time should be checked for haemoglobin disorders by routine haematological investigation and peripheral blood film examination. The patients with persistent anaemia and whose blood shows hypochromic microcytic anaemia should be advised for haemoglobin electrophoresis.KYAMC Journal Vol. 6, No.-1, Jul 2015, Page 545-549

scholarly journals Unexpected Detection of Abnormal Hemoglobin Variants During Routine HbA1c Analysis by HPLC Technique: Challenges and Opportunities in HbA1c Testing

2021 ◽  
Vol 9 (1) ◽  
pp. 15-21
Author(s):  
Neelam Narsingrao Sreedevi ◽  
Guduru Vijay Kumar ◽  
Boda Chendar ◽  
Madrol Vijaya Bhaskar ◽  
Kompella Sree Satya Sai Baba ◽  
...  
Keyword(s):  
Hemoglobin A1c ◽  
Glycated Hemoglobin ◽  
Incidental Finding ◽  
Diabetic Patients ◽  
Hb E ◽  
Challenges And Opportunities ◽  
Abnormal Hemoglobin ◽  
Diabetic Population ◽  
Hemoglobin Variants

Background: Glycated Hemoglobin A1c (HbA1c) assay is most widely used in diabetic patients for assessing long-term control of glycemia. The presence of hemoglobin variants may be an incidental finding and can interfere with HbA1c measurements. The aim of the present study is to investigate the prevalence and impact of interference of different abnormal hemoglobin variants on HbA1c measurements during routine HbA1c testing. Methods: A total of 12,092 HbA1c samples were collected from January to August 2018. HbA1c quantification was carried out on a Variant II Bio-Rad’s HPLC analyzer. Abnormal chromatograms were further analyzed using the extended-run high-pressure liquid chromatography (HPLC) analysis in the A2/F mode. Results: The samples were examined for presence of abnormal variants. Samples producing abnormal chromatograms were further analyzed in A2/F mode to characterize hemoglobin variants. Abnormal variants were identified in 126 (1%) samples, and 74 (0.59%) sickle cell traits (SCT) were the most common variant in our findings. Moreover, 30 (0.24%) cases were eluted in the variant window in A1c mode, which on further analysis were found to be Hb E & Hb D traits. Furthermore, 3 (0.02%) cases were eluted at a RT <1 min as (unknown) and identified as Hb H. Also,19 (0.15%) samples were eluted in the P3 window at different retention times. Conclusion: Observing each chromatograph after the analysis can help us in identifying silent hemoglobin variants in routine HbA1c testing. Knowledge and awareness of common hemoglobin variants affecting measurement of HbA1c is imperative to avoid reporting of falsely low HbA1c values in diabetic population.

scholarly journals THE PERIPHERAL BLOOD FILM

1968 ◽  
Vol 21 (6) ◽  
pp. 788-788
Author(s):  
A. G. Signy
Keyword(s):  
Peripheral Blood ◽  
Blood Film ◽  
Peripheral Blood Film
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