scholarly journals Analysis of Retinal Capillary Using Optical Coherence Tomographic Angiography of Unilateral Normal Tension Glaucoma

2021 ◽  
Vol 62 (10) ◽  
pp. 1397-1406
Author(s):  
Chang Woo Cho ◽  
Woo Hyun Jung ◽  
Jung Lim Kim
Keyword(s):  
Normal Control ◽  
Normal Tension Glaucoma ◽  
Normal Control Group ◽  
Control Group ◽  
Optical Coherence ◽  
Macular Area ◽  
Fellow Eye ◽  
Retinal Capillary ◽  
Capillary Plexus ◽  
Peripapillary Area

Purpose: The purpose of this study was to analyze retinal capillary parameters using optical coherence tomography angiography (OCTA) of the affected eye and the fellow eye of unilateral normal tension glaucoma (NTG) patients and compare the findings with eyes from a normal control group.Methods: A retrospective cross-sectional study was carried out on patients diagnosed with unilateral NTG (24 affected eyes and 24 fellow eyes each) and normal individuals (29 eyes, the control group). OCTA was used to measure the vascular density (VD) and perfusion density (PD) of the macular area and the peripapillary area.Results: In the superficial capillary plexus, the fellow eye group of unilateral NTG patients showed a decrease in VD of the inner-inferior and PD of the inner-inferior and outer-average peripapillary area, compared with the normal control group (p = 0.008, p < 0.001, and p = 0.001). In the affected NTG eye group, the VD (p = 0.014, p = 0.011, p < 0.001, p < 0.001, and p < 0.001) and PD (p = 0.017, p = 0.023, p < 0.001, p = 0.001, and p < 0.001) of the total, inner-average, inner-inferior, and outer-inferior peripapillary area, and the outer-inferior macular area decreased compared to the fellow eye and normal control group, as well as the VD of the outer-average peripapillary area (p = 0.010). The PD of the outer-average peripapillary area (p = 0.003); the VD (p = 0.041, p = 0.008, p = 0.006) and the PD (p = 0.013, p < 0.001, p = 0.001) of the total, inner-inferior, and outer-average macular area; and the PD of the outer-temporal macular area (p = 0.003) were lower than the normal control group. There was no difference in the VD or PD obtained from the deep capillary plexus of the macular area among the groups.Conclusions: It is useful to observe retinal capillary parameters using OCTA for patients with unilateral NTG.

scholarly journals Macular and peripapillary vessel density measurement in the evaluation of Non-proliferative Diabetic Retinopathy

2018 ◽  
Author(s):  
Dawei Yang ◽  
Dan Cao ◽  
Honghua Yu ◽  
Xiaohong Yang ◽  
Zhongning Huang ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Diabetic Retinopathy ◽  
Normal Control ◽  
Optical Coherence Tomography Angiography ◽  
Vessel Density ◽  
Optical Coherence ◽  
Macular Area ◽  
Rnfl Thickness ◽  
Capillary Plexus ◽  
Peripapillary Area

Abstract Purpose: To compare vessel density in macular and peripapillary area between control subjects and patients with non-proliferative diabetic retinopathy (NPDR) using optical coherence tomography angiography (OCTA) and to evaluate the association between RNFL thickness and different stage of diabetic retinopathy. Methods: A total of 170 eyes (normal control, 43; mild NPDR, 43; moderate NPDR, 42; severe NPDR, 42) underwent OCTA imaging. Optical coherence tomography angiographic parameters were vessel densities in superficial capillary plexus (SCP), deep capillary plexus (DCP) in macular area and peripapillary area. Results: The reduction of vessel density of SCP and DCP in macular area, peripapillary area as well as RNFL thickness were correlated with increasing severity of DR. Vessel density of SCP and DCP in macular area, peripapillary area and FD300 in NPDR groups decreased as compared to normal control (P<0.001). Vessel density of DCP shows better ability to identify the severity of DR (sensitivity, and specificity of 88.1%, and 85.2%, respectively) than in FD 300, vessel density of SCP in macular area and peripapillary area Conclusion: Macular and peripapillary vessel density as well as RNFL thickness were significantly decreased in different stage of NPDR compared to normal controls. Vessel density in DCP could be an objective and sensitive indicator for monitoring progression of DR. OCTA might be clinically useful to evaluate microvascular and microstructural alterations in macula and ONH, thus providing a new method to study the course of DR. Key Words: Diabetic retinopathy, optical coherence tomography angiography, vessel density, RNFL thickness, FD 300

scholarly journals The Application Value of Optical Coherence Tomography in Primary Acute Angle Closure

2015 ◽  
Vol 4 (4) ◽  
pp. 42
Author(s):  
Li Gao ◽  
Xiaodong Tang
Keyword(s):  
Optical Coherence Tomography ◽  
Normal Control ◽  
Diagnostic Value ◽  
Acute Angle ◽  
Normal Control Group ◽  
Angle Closure ◽  
Control Group ◽  
Optical Coherence ◽  
Fiber Layer ◽  
Inspection Method

<p><strong>Objective: </strong>To investigate the diagnostic value of optical coherence tomography (OCT) in the diagnosis of acute primary angle-closure glaucoma (APACG). <strong>Method: </strong>52 eyes were chosen for OCT applications at different stages of APACG and compared with the normal control group. The peripapillary retinal nerve fiber layer (RNFL) between the two groups was compared. <strong>Results: </strong>The average thickness of RNFL APACG was significantly thicker during the earlier phase of treatment and thinner after 6 months of treatment as compared to the normal control group. <strong>Conclusion: </strong>OCT can reflect the change in the thickness of glaucoma RNFL, and can provide more information for clinical early diagnosis of glaucoma and is a worthy application of the inspection method.</p>

scholarly journals Macular and peripapillary vessel density measurement in the evaluation of Non-proliferative Diabetic Retinopathy

2019 ◽  
Author(s):  
Dawei Yang ◽  
Dan Cao ◽  
Honghua Yu ◽  
Xiaohong Yang ◽  
Zhongning Huang ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Diabetic Retinopathy ◽  
Normal Control ◽  
Proliferative Diabetic Retinopathy ◽  
Vessel Density ◽  
Macular Area ◽  
Control Subjects ◽  
Rnfl Thickness ◽  
Capillary Plexus ◽  
Peripapillary Area

Abstract Purpose: To compare vessel density in macular and peripapillary area between control subjects and patients with non-proliferative diabetic retinopathy (NPDR) using optical coherence tomography angiography (OCTA) and to evaluate the association between RNFL thickness and different stage of diabetic retinopathy. Methods: A total of 170 eyes (normal control, 43; mild NPDR, 43; moderate NPDR, 42; severe NPDR, 42) underwent OCTA imaging. Optical coherence tomography angiographic parameters were vessel densities of superficial capillary plexus (SCP), deep capillary plexus (DCP) in macular area and peripapillary area. Results: Vessel density of SCP and DCP in macular area, peripapillary area as well as RNFL thickness were 53.13%±3.03%, 52.07%±2.28%, 53.78%±3.66% and 128.86μm±11.32μm, respectively, in control subjects; 50.24%±3.81%, 47.40%±45.02%, 46.50%±6.03% and 124μm±13.97μm, respectively, in mild NPDR; 46.80%±5.23%; 44.39%±3.99%; 44.64%±4.23%; 121.02μm±20.86μm, respectively, in moderate NPDR; 42.82%±5.46%, 42.34%±5.14%, 43.16%±4.47%, 118.60μm±21.91μm in severe NPDR. The reduction of vessel density of SCP and DCP in macular area, peripapillary area as well as RNFL thickness were correlated with increasing severity of DR. Vessel density of SCP and DCP in macular area, peripapillary area and foveal density 300 (FD 300) in normal control were significantly higher than that of mild, moderate and severe NPDR groups. (all P<0.001). Vessel density of DCP shows better ability to identify the severity of DR (0.913; 95% CI=0.867-0.958; cut off value:0.75) than FD 300, vessel density of SCP in macular area and peripapillary area. Conclusion: Macular and peripapillary vessel density as well as RNFL thickness decreased as DR progresses. Vessel density in DCP could be an objective and sensitive indicator for monitoring progression of DR. OCTA might be clinically useful to evaluate microvascular and microstructural alterations in macula and optive nerve head (ONH), thus providing a new method to study the course of DR.

scholarly journals Macular and peripapillary vessel density measurement in the evaluation of Non-proliferative Diabetic Retinopathy

2019 ◽  
Author(s):  
Dawei Yang ◽  
Dan Cao ◽  
Honghua Yu ◽  
Xiaohong Yang ◽  
Zhongning Huang ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Diabetic Retinopathy ◽  
Normal Control ◽  
Proliferative Diabetic Retinopathy ◽  
Vessel Density ◽  
Macular Area ◽  
Control Subjects ◽  
Rnfl Thickness ◽  
Capillary Plexus ◽  
Peripapillary Area

Abstract Purpose: To compare vessel density in macular and peripapillary area between control subjects and patients with non-proliferative diabetic retinopathy (NPDR) using optical coherence tomography angiography (OCTA) and to evaluate the association between RNFL thickness and different stage of diabetic retinopathy. Methods: A total of 170 eyes (normal control, 43; mild NPDR, 43; moderate NPDR, 42; severe NPDR, 42) underwent OCTA imaging. Optical coherence tomography angiographic parameters were vessel densities of superficial capillary plexus (SCP), deep capillary plexus (DCP) in macular area and peripapillary area. Results: Vessel density of SCP and DCP in macular area, peripapillary area as well as RNFL thickness were 53.13%±3.03%, 52.07%±2.28%, 53.78%±3.66% and 128.86μm±11.32μm, respectively, in control subjects; 50.24%±3.81%, 47.40%±45.02%, 46.50%±6.03% and 124μm±13.97μm, respectively, in mild NPDR; 46.80%±5.23%; 44.39%±3.99%; 44.64%±4.23%; 121.02μm±20.86μm, respectively, in moderate NPDR; 42.82%±5.46%, 42.34%±5.14%, 43.16%±4.47%, 118.60μm±21.91μm in severe NPDR. The reduction of vessel density of SCP and DCP in macular area, peripapillary area as well as RNFL thickness were correlated with increasing severity of DR. Vessel density of SCP and DCP in macular area, peripapillary area and foveal density 300 (FD 300) in normal control were significantly higher than that of mild, moderate and severe NPDR groups. (all P<0.001). Vessel density of DCP shows better ability to identify the severity of DR (0.913; 95% CI=0.867-0.958; cut off value:0.75) than FD 300, vessel density of SCP in macular area and peripapillary area. Conclusion: Macular and peripapillary vessel density as well as RNFL thickness decreased as DR progresses. Vessel density in DCP could be an objective and sensitive indicator for monitoring progression of DR. OCTA might be clinically useful to evaluate microvascular and microstructural alterations in macula and optive nerve head (ONH), thus providing a new method to study the course of DR.

scholarly journals Study on potential differentially expressed genes in stroke by bioinformatics analysis

2021 ◽  
Author(s):  
Xitong Yang ◽  
Pengyu Wang ◽  
Shanquan Yan ◽  
Guangming Wang
Keyword(s):  
Gene Expression ◽  
Differentially Expressed Genes ◽  
Normal Control ◽  
Enrichment Analysis ◽  
Normal Control Group ◽  
Control Group ◽  
Ppi Network ◽  
Hub Genes ◽  
Pathway Enrichment ◽  
Key Genes

AbstractStroke is a sudden cerebrovascular circulatory disorder with high morbidity, disability, mortality, and recurrence rate, but its pathogenesis and key genes are still unclear. In this study, bioinformatics was used to deeply analyze the pathogenesis of stroke and related key genes, so as to study the potential pathogenesis of stroke and provide guidance for clinical treatment. Gene Expression profiles of GSE58294 and GSE16561 were obtained from Gene Expression Omnibus (GEO), the differentially expressed genes (DEGs) were identified between IS and normal control group. The different expression genes (DEGs) between IS and normal control group were screened with the GEO2R online tool. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of the DEGs were performed. Using the Database for Annotation, Visualization and Integrated Discovery (DAVID) and gene set enrichment analysis (GSEA), the function and pathway enrichment analysis of DEGS were performed. Then, a protein–protein interaction (PPI) network was constructed via the Search Tool for the Retrieval of Interacting Genes (STRING) database. Cytoscape with CytoHubba were used to identify the hub genes. Finally, NetworkAnalyst was used to construct the targeted microRNAs (miRNAs) of the hub genes. A total of 85 DEGs were screened out in this study, including 65 upward genes and 20 downward genes. In addition, 3 KEGG pathways, cytokine − cytokine receptor interaction, hematopoietic cell lineage, B cell receptor signaling pathway, were significantly enriched using a database for labeling, visualization, and synthetic discovery. In combination with the results of the PPI network and CytoHubba, 10 hub genes including CEACAM8, CD19, MMP9, ARG1, CKAP4, CCR7, MGAM, CD79A, CD79B, and CLEC4D were selected. Combined with DEG-miRNAs visualization, 5 miRNAs, including hsa-mir-146a-5p, hsa-mir-7-5p, hsa-mir-335-5p, and hsa-mir-27a- 3p, were predicted as possibly the key miRNAs. Our findings will contribute to identification of potential biomarkers and novel strategies for the treatment of ischemic stroke, and provide a new strategy for clinical therapy.

scholarly journals The role of quantitative deep capillary plexus in the pathogenesis of type 3 macular neovascularization: an optical coherence tomography angiography study

2021 ◽  
Author(s):  
Marina Concilio ◽  
Federica Fossataro ◽  
Daniela Montorio ◽  
Mariapaola Giordano ◽  
Gilda Cennamo
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence Tomography Angiography ◽  
Early Stage ◽  
Study Groups ◽  
Control Group ◽  
Main Role ◽  
Optical Coherence ◽  
Capillary Plexus ◽  
Type 3

Abstract Purpose To quantitatively investigate the role of deep capillary plexus (DCP) in patients affected by type 3 macular neovascularization (MNV), compared to patients with reticular pseudodrusen (RPD) eyes and healthy controls, using optical coherence tomography angiography (OCTA). Methods In this prospective observational study, a total of seventy-eight eyes of 78 patients were included. Group 1 consisted of 40 eyes of 40 patients with stage 1 of type 3 MNV (22 males, 18 females, mean age 73.7, SD ± 6.60) and group 2 included 38 eyes of 38 patients with RPD (17 males, 21 females, mean age 73.2, SD ± 4.55). The control group included 40 eyes of 40 healthy subjects (20 males, 20 females, mean age 71.4, SD ± 6.36 years). We evaluated the retinal vessel density (VD) of superficial capillary plexus (SCP) and deep capillary plexus (DCP) using OCTA. Results Patients with diagnosis of type 3 MNV showed statistically lower values of VD in DCP with respect to controls and to RPD group (p < 0.001), while there were no statistical differences between RPD and control group in macular region. No significant differences in VD of SCP were detected among the three study groups. Conclusion OCTA provides a reproducible, non-invasive detailed quantitative analysis of retinal vascular features and changing in early-stage type 3 MNV patients, which allowed to shed the light on the main role of DCP ischemia in the development of type 3 MNV.

Effect of GDM on the Expression of MT1-MMP and u-PA in Glioma Cells

2014 ◽  
Vol 1033-1034 ◽  
pp. 220-223
Author(s):  
Xue Mei Han ◽  
Li Bo Wang ◽  
Ni Ni Li ◽  
Song Yan Liu
Keyword(s):  
Normal Control ◽  
Glioma Cell ◽  
Fetal Bovine Serum ◽  
Glioma Cells ◽  
Normal Control Group ◽  
Control Group ◽  
Rt Pcr ◽  
Glioma Cell Lines ◽  
Fetal Bovine ◽  
Identify Gene Expression

To examine the effect of GDM on the expression of MT1-MMP and u-PA genes in glioma cells. Glioma cell lines U251 and U87 were cultured in DMEM medium supplemented with 10% fetal bovine serum. RT-PCR was used to identify gene expression level. The level of u-PA mRNA was up-regulated significantly in the HGF group compared with the normal control group (P<0.05). The expression of MT1-MMP and u-PA was significantly lower in the GDM group than in the normal control and HGF groups (P<0.05). The expression of u-PA in the HGF+GDM group was down-regulated significantly compared with the normal control and HGF groups (P<0.05).GDM can inhibit expression of both MT1-MMP and u-PA in glioma cells.

Identification of Hub Genes Associated with Development of Lung Adenocarcinoma by Integrated Bioinformatics Analysis

2021 ◽  
Author(s):  
Jinglei Li ◽  
Wei Hou
Keyword(s):  
Gene Expression ◽  
Survival Analysis ◽  
Network Analysis ◽  
Lung Adenocarcinoma ◽  
Normal Control ◽  
Gene Expression Analysis ◽  
Normal Control Group ◽  
Control Group ◽  
Differential Gene Expression Analysis ◽  
Differential Gene

Abstract Purpose: Lung adenocarcinoma (LUAD) has high heterogeneity and poor prognosis, posing a major challenge to human health worldwide. Therefore, it is necessary to improve our understanding of the molecular mechanism of LUAD in order to be able to better predict its prognosis and develop new therapeutic strategies for target genes.Methods: The Cancer Genome Atlas and Gene Expression Omnibus, were selected to comprehensively analyze and explore the differences between LUAD tumors and adjacent normal tissues. Critical gene information was obtained through weighted gene co-expression network analysis (WGCNA), differential gene expression analysis, and survival analysis.Results: Using WGCNA and differential gene expression analysis, 29 differentially expressed genes were screened. The functional annotation analysis showed these genes to be mainly concentrated in heart trabecula formation, regulation of inflammatory response, collagen-containing extracellular matrix, and metalloendopeptidase inhibitor activity. Also, in the protein–protein interaction network analysis, 10 central genes were identified using Cytoscape's CytoHubba plug-in. The expression of CDH5, TEK, TIMP3, EDNRB, EPAS1, MYL9, SPARCL1, KLF4, and TGFBR3 in LUAD tissue was found to be lower than that in the normal control group, while the expression of MMP1 in LUAD tissue was higher than that in the normal control group. According to survival analysis, the low expression of MYL9 and SPARCL1 was correlated with poor overall survival in patients with LUAD. Finally, through the verification of the Oncomine database, it was found that the expression levels of MYL9 and SPARCL1 were consistent with the mRNA levels in LUAD samples, and both were downregulated.Conclusion: Two survival-related genes, MYL9 and SPARCL1, were determined to be highly correlated with the development of LUAD. Both may play an essential role in the development LUAD and may be potential biomarkers for its diagnosis and treatment in the future.

Foveal avascular zone in oculocutaneous albinism

2021 ◽  
Vol 14 (5) ◽  
pp. e240208
Author(s):  
Hana A Mansour ◽  
Sami Uwaydat ◽  
Muhamad H Yunis ◽  
Ahmad M Mansour
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence Tomography Angiography ◽  
Oculocutaneous Albinism ◽  
Foveal Avascular Zone ◽  
Optical Coherence ◽  
Retinal Capillary ◽  
Avascular Zone ◽  
Capillary Plexus ◽  
Foveal Hypoplasia

Optical coherence tomography angiography imaging in two patients with oculocutaneous albinism, one with severe nystagmus, showed persistence of both the superficial and the deep retinal capillary plexus adding another vascular feature to the foveal hypoplasia.

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