scholarly journals Investigation of gastrintestinal parasites in rabbits of the species Oryctolagus cuniculus in the Northwest region of São Paulo, Brazil

2021 ◽  
Vol 10 (10) ◽  
pp. e468101019130
Author(s):  
Débora Regina Romualdo da Silva ◽  
Sandra Valéria Inácio ◽  
Walter Bertequini Nagata ◽  
Carolina Beatriz Baptista ◽  
Ana Paula Fernezi Bassi ◽  
...  

Enteroparasites can be pathogenic for the rabbit population and for this reason, we have investigated its occurrence in bioterium of rabbits. The sample group was composed of 103 adult males of the species Oryctolagus cuniculus and New Zealand breed. Fecal samples (n = 103) were processed using the Willis Techniques (Fluctuation in saturated sodium chloride solution with specific density of 1.20 g/mL) and Faust (Centrifugal-flotation in zinc sulphate with density of 1.18 g/ml). The diameters of larger and smaller sizes of the sporocysts and sporozoites of the coccidia were measured by means of optical microscopy using the program CellSens 1.17_00_en_Rhine_13Feb2017. The Faust Technique showed positivity of 39.74% (31/78) for Eimeria spp, while in Willis Technique, 35.90% (28/78) was observed for Eimeria spp, being the species Eimeria perforans and in 51.28% (47/78) of them there was no parasitism. We identified, for the first time, the occurrence of the specie E. perforans in rabbits of the species O. cuniculus, New Zealand breed kept in a bioterium. Therefore, it is extremely important for the implantation of parasitic control of rabbits kept in bioterium for scientific purposes, the monitoring of possible infections by means of periodic coproparasitological exams.

2013 ◽  
Vol 355 (6) ◽  
pp. 1077-1082 ◽  
Author(s):  
Liuqun Gu ◽  
Ting Lu ◽  
Mingyun Zhang ◽  
Lijuan Tou ◽  
Yugen Zhang

Author(s):  
Bharat Sontakke ◽  
Vishwajit Ravindra Deshmukh ◽  
C. Kirubhanand ◽  
T.S. Gugapriya ◽  
Gayatri Muthiyan ◽  
...  

Art of embalming as practised by Egyptian about 3000 years ago transformed into embalming science of modern ages with the use of formaldehyde as a preservative solution. Subsequently, the search for ideal embalming preservative solution continues to date because of the health hazards related to formaldehyde preservation of cadavers. Alternative preservative methods and solutions suitable for making different skill training models with the specific requirement of pliability have also experimented. The literature had documented various solutions like Thiel’s solution and technique, phenoxyethanol preservation, saturated sodium chloride solution, cryopreservation, N-Vinyl-2-pyrrolidone, Ethanol–glycerin and Fix 4life solution as alternatives to formaldehyde preservation. This review is an attempt to have an overview comparison of all the recent alternate embalming methods applicable for developing skill training cadaveric models with an aim of reducing formaldehyde usage in preservation.


1985 ◽  
Vol 68 (4) ◽  
pp. 750-753
Author(s):  
Promode C Bardalaye ◽  
Willis B Wheeler

Abstract Residue analysis of the herbicide prometryn (2,4-bis(isopropylamino)- 6-methylthio-l,3,5-triazine) is widely known, but an analytical method for determining its metabolities or degradation products in addition to the parent chemical has not yet been reported in the literature. The procedure reported here is for the extraction and determination of prometryn and 2 metabolites, 2-amino-4-isopropylamino-6-methylthio- l,3,5-triazine and 2,4-diamino-6-methylthio-l,3,5-triazine, in parsley. Crops were extracted with 2-propanol followed by concentration of the extract and partitioning with a minimum amount of hexane in the presence of a large excess of water to remove most of the green pigment. The aqueous phase was divided into 2 equal halves: (A) Onehalf portion was partitioned with dichloromethane in the presence of saturated sodium chloride solution, the dichloromethane phase was separated, and the aqueous phase was discarded. The organic solvent was evaporated, and the contents were reconstituted in petroleum ether before prometryn analysis. (B) The other half was made slightly alkaline with ammonium hydroxide solution and was partitioned with ethyl acetate in the presence of saturated sodium chloride solution. The ethyl acetate phase was concentrated, centrifuged to remove any turbidity, and analyzed for the 2 metabolities above. Fused silica capillary gas chromatography (GC) with nitrogen-phosphorus (N-P) detection was used for quantitation. The limit of detection was 0.05 mg/kg for all the compounds examined. Recoveries from fortified parsley samples ranged from 59 to 73% at fortification levels of 0.05 to 1.0 mg/kg.


1970 ◽  
Vol 119 (1) ◽  
pp. 85-88 ◽  
Author(s):  
N. Ressler ◽  
S. Lee

Glyceraldehyde 3-phosphate dehydrogenase exists in two different forms in various human tissue preparations. One of them is exhibited, after starch-gel electrophoresis, by a rapidly migrating or `fast' band and the other by a `slow' band. The proportion of the total activity in each of the two forms is characteristic of the type of tissue. A particulate fraction, obtained after centrifugation of homogenates, inhibits the enzyme activity and tends to convert the slow band into a fast one. The conversion is reversible. The fast band can also be converted into the slow one by addition of NAD+ or ADP, or by dialysis against saturated sodium chloride solution. Conversions occur with the purified enzyme as well as with crude homogenates. The relevance of these findings to previous investigations and to glycolytic control mechanisms are discussed.


1978 ◽  
Vol 61 (5) ◽  
pp. 1089-1091
Author(s):  
Carl Ponder

Abstract A fluorometric method is described for determining indole in shrimp. The indole is extracted with n-hexane, partitioned into a methanol- saturated sodium chloride solution ( 9 + 1 ), and determined fluoromctrically. The detection limit of the method is 0.04 μg indole/g shrimp. Quantitative analytical data are presented for indole in samples of shrimp determined by the fluorometric method and the AOAC official colorimetric and gas-liquid chromatographic procedures. The correlation coefficient between the data of the fluorometric and colorimetric methods was 0.96. Indole recovered from 25 g samples of fresh shrimp spiked with 6 and 12 μg indole/25 g ranged from 97 to 106%. The fluorescence response is linear in the range of 1 to 25 μg indole/100 ml methanol solution and no significant change is noted in solutions kept in the dark 18 hr.


CrystEngComm ◽  
2018 ◽  
Vol 20 (3) ◽  
pp. 334-339
Author(s):  
Eleanor R. Townsend ◽  
Willem J. P. van Enckevort ◽  
Jan A. M. Meijer ◽  
Elias Vlieg

The creeping pattern observed when a saturated sodium chloride solution containing 1% (w/w) methylglycine diacetamide is allowed to dry at ambient temperature.


Parasitology ◽  
1978 ◽  
Vol 77 (1) ◽  
pp. 33-39 ◽  
Author(s):  
Nicholas G. Ryley ◽  
John F. Ryley

SummaryUsing the ability to sporulate as a measure of viability, the effects of exposure of unsporulated oocysts of 10 species of coccidia of chickens, rabbits and cattle to saturated NaCl solution has been studied. Although appreciable deformation and collapse of the oocyst occurred after 1–2 days contact, the effect was reversible after washing free from salt and incubating. Some reduction in ability to sporulate following several days contact with saturated salt was noted in most species, although no effect was seen with Eimeria stiedai following 7 days exposure, Eimeria tenella was one of the more sensitive species studied. Culture titration experiments in chickens with E. tenella indicated that oocysts which had sporulated following prolonged exposure to salt were in no way inferior in virulence or ability to retain virulence on prolonged storage to oocysts prepared with minimal contact with salt. No evidence was obtained to contra-indicate the use of salt-flotation methods for the separation of oocysts from faeces.


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