scholarly journals Chlorella sorokiniana cultivation in cheese whey for β-galactosidase production

2021 ◽  
Vol 10 (12) ◽  
pp. e468101220727
Author(s):  
Maicon Jhonatan Bueno do Amaral Santos ◽  
Diva de Souza Andrade ◽  
Alessandra Bosso ◽  
Mayara Mari Murata ◽  
Luiz Rodrigo Ito Morioka ◽  
...  
Keyword(s):  
Physicochemical Parameters ◽  
Cheese Whey ◽  
High Capacity ◽  
Chlorella Sorokiniana ◽  
Cultivation Condition ◽  
High Biomass ◽  
Box Behnken Design ◽  
Biomass Yields ◽  
Biotechnological Processes ◽  
Beta Galactosidase

Biotechnological processes with microalgae with the aim to achieve high biomass yields must choose the appropriate nutrients and physicochemical parameters, taking into account the specific characteristics of each species to determine the basic needs for its growth. In the present study, the better growth condition of Chlorella sorokiniana IPR 7104 was optimized to reach the maximum beta-galactosidase production. The cheese whey concentration (%), temperature (˚C) and pH were factors investigated and a Box-Behnken Design (BBD) approach was implemented using Statistica 7.0 software. We observed that the cultivation condition to Chlorella sorokiniana IPR 7104 was the heterotrophic, which showed the major enzymatic activity, consequently a lower residual lactose content. Under heterotrophic conditions (without light) the β-galactosidase activity increased linearly until the 8th day. Biomass production grew linearly on the 12th day. The microalgae consumed 89.6% of lactose in 3 days, showing a high capacity to metabolize this disaccharide, through β-galactosidase synthesis. The maximum β-galactosidase production by Chlorella sorokiniana IPR 7104, in heterotrophic conditions and using cheese whey as carbon source, is obtained using the following conditions: 30°C temperature, concentration of ethanol at 20% and time of 4 min.

scholarly journals Optimization of Cultivation Condition of Newly Isolated Strain Chlorella Sorokiniana pa.91 for CO2 Bio-Fixation and Nutrients Removal From Real Municipal Wastewater: Impact of Temperature and Light Intensity

2021 ◽  
Author(s):  
Poone Yaqoubnejad ◽  
Hassan Aminirad ◽  
Mohsen Taghavijeloudar
Keyword(s):  
Municipal Wastewater ◽  
Removal Rate ◽  
High Capacity ◽  
Biomass Concentration ◽  
Biomass Productivity ◽  
Batch Cultivation ◽  
Culture Conditions ◽  
Chlorella Sorokiniana ◽  
Synthetic Wastewater ◽  
Cultivation Condition

Abstract The cultivation conditions of a newly isolated strain Chlorella sorokiniana pa.91 were optimized for the first time by performing sixty batch cultivation experiments at various temperatures (20, 25, 30 and 35 °C) and light intensities (1000, 3000, 4000, 5000 and 7000 Lux) in three different culture mediums of BG-11, real settled municipal wastewater (RMWW) and synthetic wastewater (SWW). Additionally, to evaluate the capability of C. sorokiniana pa.91 in CO2 bio-fixation and wastewater treatment, the microalgae was cultivated in a flat-plate photobioreactor (CO2 = 16% and 0.6 vvm aeration) under the optimal condition. The optimization results suggested that at the culture conditions of 30 °C, 4000 Lux and RMWW (COD 211 mgL-1) microalgae had the best performance in growth and biomass productivity. Maximum biomass concentration and productivity of 3.21 gL-1 and 0.31 gL-1d-1were achieved, respectively, by cultivation of C. sorokiniana pa.91 in the photobioreactor under the optimized condition. Experimental results showed that C. sorokiniana pa.91 has a high capacity of CO2 bio-fixation (0.59 mgL-1d-1) and CO2 removal rate (35.6 %). Moreover, using C. sorokiniana pa.91 could efficiently remove 74% of NH3, 93% of NO3-, 83% of PO4-3 and 76% of COD from real municipal wastewater after eight days of cultivation in the photobioreactor.

Substrate consumption and beta-galactosidase production by Saccharomyces fragilis IZ 275 grown in cheese whey as a function of cell growth rate

2019 ◽  
Vol 21 ◽  
pp. 101335
Author(s):  
Alessandra Bosso ◽  
Ana Caroline Iglecias Setti ◽  
Adriana Bosso Tomal ◽  
Samuel Guemra ◽  
Luiz Rodrigo Ito Morioka ◽  
...  
Keyword(s):  
Growth Rate ◽  
Cell Growth ◽  
Cheese Whey ◽  
Cell Growth Rate ◽  
Substrate Consumption ◽  
Beta Galactosidase

scholarly journals Direct-Acid-Set Cottage Cheese Whey as a Base for a Shelf-Stable Athletic-Type Drink1

1984 ◽  
Vol 47 (1) ◽  
pp. 53-57 ◽  
Author(s):  
K. L. CRIPPEN ◽  
I. J. JEON
Keyword(s):  
Calcium Hydroxide ◽  
Potassium Hydroxide ◽  
Cheese Whey ◽  
Potassium Hydroxide Solution ◽  
Cottage Cheese ◽  
Additional Protein ◽  
The Stability ◽  
Shelf Stable ◽  
Sodium And Potassium ◽  
Beta Galactosidase

A shelf-stable athletic-type beverage was developed from direct-acid-set cottage cheese whey. First, the pH of the whey was adjusted to 5.2 with a saturated potassium hydroxide solution. The whey was heated with stirring to 90°C and held for 10 min to coagulate the protein, which then was removed by filtering or centrifuging. Calcium hydroxide was added to increase the pH to 5.6, and then potassium hydroxide was added to bring the pH up further to 6.5. The whey was filtered or centrifuged again to remove the cloudiness caused by addition of calcium hydroxide and additional protein precipitation. Beta-galactosidase was added and whey was held at 5°C for 18 h to hydrolyze the lactose. Then, one part water was mixed with two parts whey before saturated citric acid was added to make an acceptable orange-flavored beverage. The beverage then was heated to 88°C and stored in 8-fl. oz. bottles capped with Teflon-lined closures. The levels of electrolytes, such as sodium and potassium, in this product were similar to those in commercially available athletic-type drinks. In two separate trials, involving 28 persons each, the whey-based drink, when compared with a commercial product, was preferred 64% and 46% of the time, respectively. During storage some of the added sucrose was hydrolyzed into glucose and fructose; however, a taste panel did not detect a change in sweetness in the stored products. The heat process used (88°C for 5 min) appeared to be adequate for commercial sterility. The stability of the product during storage was good and estimated to be longer than 6 months. Ingredient cost of the whey-based athletic-type drink was $0.14 per 32 fl. oz.

Use of Organic and Biological Fertilizers as Strategies to Improve Crop Biomass, Yields and Physicochemical Parameters of Soil

2019 ◽  
pp. 247-288 ◽  
Author(s):  
Abdelilah Meddich ◽  
Khalid Oufdou ◽  
Abderrahim Boutasknit ◽  
Anas Raklami ◽  
Abdelilah Tahiri ◽  
...  
Keyword(s):  
Physicochemical Parameters ◽  
Biomass Yields ◽  
Crop Biomass

High biomass genotypes in spring barley

1985 ◽  
Vol 105 (1) ◽  
pp. 73-78 ◽  
Author(s):  
P. R. Hanson ◽  
T. J. Riggs ◽  
S. J. Klose ◽  
R. B. Austin
Keyword(s):  
Dry Matter ◽  
Spring Barley ◽  
High Ratio ◽  
High Yield ◽  
Resistant Variety ◽  
High Biomass ◽  
Breeding Lines ◽  
Biomass Yields ◽  
The Mean ◽  
Support Treatment

SUMMARYThree experiments were carried out to measure grain and biomass yields of spring barley breeding lines and varieties. Leaf diseases were controlled by applying proprietary fungicides and in some treatments plants were supported by nets to prevent lodging. The trials, grown in 1982 and 1983, compared the grain and biomass yields of 14 breeding lines and two varieties with and without support. In a third experiment, comparing three lines and the varieties Triumph and Egmont, all the plots were supported and in addition each experimental plot was flanked by guard plots of the same genotype to minimize inter-plot competition.In the supported plots of all three experiments, three breeding lines, SB 543–3, SB 71–2 and HSB 258–93 produced significantly greater biomass yields than the mean of the varieties Triumph and Egmont. All three breeding lines were taller and had lower ratios of grain to grain + straw than the controls. Measurements were made of the accumulation of dry matter, chlorophyll content at anthesis and date of ear emergence but no single factor could be identified which was associated with increased biomass in these lines.The grain yield of the semi-dwarf, lodging-resistant variety Triumph was reduced by an average of 0·24 t/ha by the support treatment compared with the non-supported control. The results from the third experiment suggested that the yield of Triumph was reduced by inter-plot competition by about 10% in relation to the other entries in the trial.It is suggested that the high biomass lines identified here could be used as parents in a breeding programme to produce varieties with high yield if this high biomass could be combined with a high ratio of grain to above-ground dry-matter yield.

Chlorella sorokiniana: Effect of Nitrate Replete Concentration on Biomass Yield, Cell and Nitrate Concentration

2019 ◽  
Vol 797 ◽  
pp. 365-372
Author(s):  
Nor Azalina Zakariah ◽  
Norazah Abd Rahman ◽  
Noor Amelina Abdul Rahim
Keyword(s):  
Optical Density ◽  
Lipid Content ◽  
Biomass Yield ◽  
Cell Concentration ◽  
Nitrate Concentration ◽  
Basal Medium ◽  
Chlorella Sorokiniana ◽  
High Biomass ◽  
Nitrate Concentrations ◽  
High Biomass Yield

Effect of various nitrate replete concentrations have been investigated in terms of biomass yield, cell and nitrate concentrations. Media used in this study is Bold’s basal medium which contains nitrate that act as nutrient. Its concentrations have been varied for obtaining the high biomass yield. The nitrate concentrations used were 30 mg nitrate/L, 35 mg nitrate/L, 40 mg nitrate/L, 45 mg nitrate/L and 50 mg nitrate/L) with 30 mg nitrate/L as a control. Microalgae Chlorella sp. is cultured and aerated in a Schott bottle with presents of light. Results showed that the best concentration to obtain highest biomass yield was 45 mg nitrate/L. It also gave the highest optical density reading at day 9 with 2.100 ± 0.070 and showed the highest cell concentration with 703 ± 29 x 106 cells/mL. Dry algae produced by this concentration after the end of the cycle was 291 ± 9 mg which was the highest compared to other concentration. It is suggested that as the biomass yield is increased by using 45 mg nitrate/L concentration, other methods to increase lipid content can be paired with nitrate replete method and can be further studied in the future.

Sign in / Sign up

Export Citation Format

Share Document