scholarly journals Metagenomic identification of a nodavirus and a circular ssDNA virus in semi-purified viral nucleic acids from the hepatopancreas of healthy Farfantepenaeus duorarum shrimp

2013 ◽  
Vol 105 (3) ◽  
pp. 237-242 ◽  
Author(s):  
TFF Ng ◽  
S Alavandi ◽  
A Varsani ◽  
S Burghart ◽  
M Breitbart
Keyword(s):  
Nucleic Acids ◽  
Ssdna Virus ◽  
Farfantepenaeus Duorarum ◽  
Circular Ssdna

scholarly journals Novel ssDNA virus recovered from estuarine Mollusc (Amphibola crenata) whose replication associated protein (Rep) shares similarities with Rep-like sequences of bacterial origin

2013 ◽  
Vol 94 (5) ◽  
pp. 1104-1110 ◽  
Author(s):  
Anisha Dayaram ◽  
Sharyn Goldstien ◽  
Peyman Zawar-Reza ◽  
Christopher Gomez ◽  
Jon S. Harding ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Ssdna Virus ◽  
Organic Detritus ◽  
Bacterial Origin ◽  
The Past ◽  
Circular Ssdna ◽  
Human Pollution ◽  
Micro Organisms ◽  
Ssdna Viruses

Over the past couple of years highly diverse novel ssDNA viruses have been discovered. Here, we present the first ssDNA virus, Gastropod-associated circular ssDNA virus (GaCSV), recovered from a mollusc Amphibola crenata Martyn 1784, which is a deposit feeder that grazes micro-organisms and organic detritus on the surface of tidal mudflats. The GaCSV (2351 nt) genome contains two large bidirectionally transcribed ORFs. The smaller ORF (874 nt) has similarities to viral replication-associated protein (Rep) sequences of some bacteria and circoviruses, whereas the larger ORF (955 nt) does not relate to any sequences in public databases and we presume it potentially encodes the capsid protein. Phylogenetic analysis shows that the GaCSV Rep clusters with Rep-like sequences of bacterial origin, highlighting the role of ssDNA viruses in horizontal gene transfer. The occurrence of previously unknown viruses in organisms associated with human pollution is a relatively unexplored field.

Diagnostic methods for the detection of faba bean necrotic yellows virus, a circular ssDNA virus

EPPO Bulletin ◽  
1995 ◽  
Vol 25 (1-2) ◽  
pp. 329-336 ◽  
Author(s):  
L. KATUL ◽  
H. J. VETTEN ◽  
D.-E. LESEMANN ◽  
E. MAISS ◽  
K. M. MAKKOUK
Keyword(s):  
Faba Bean ◽  
Diagnostic Methods ◽  
Ssdna Virus ◽  
Circular Ssdna

scholarly journals An experimental strategy for preparing circular ssDNA virus genomes for next-generation sequencing

2021 ◽  
pp. 114405
Author(s):  
Catherine D. Aimone ◽  
J. Steen Hoyer ◽  
Anna E. Dye ◽  
David O. Deppong ◽  
Siobain Duffy ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Ssdna Virus ◽  
Next Generation ◽  
Experimental Strategy ◽  
Circular Ssdna ◽  
Virus Genomes ◽  
Generation Sequencing

A novel circular ssDNA virus of the phylum Cressdnaviricota discovered in metagenomic data from otter clams (Lutraria rhynchaena)

2020 ◽  
Vol 165 (12) ◽  
pp. 2921-2926
Author(s):  
Oanh T. P. Kim ◽  
Yuki Kagaya ◽  
Hoang S. Tran ◽  
Ryuhei Minei ◽  
Trang T. H. Tran ◽  
...  
Keyword(s):  
Metagenomic Data ◽  
Ssdna Virus ◽  
Circular Ssdna

scholarly journals Dragonfly cyclovirus, a novel single-stranded DNA virus discovered in dragonflies (Odonata: Anisoptera)

2011 ◽  
Vol 92 (6) ◽  
pp. 1302-1308 ◽  
Author(s):  
Karyna Rosario ◽  
Milen Marinov ◽  
Daisy Stainton ◽  
Simona Kraberger ◽  
Elizabeth J. Wiltshire ◽  
...  
Keyword(s):  
Viral Metagenomics ◽  
Nucleotide Identity ◽  
Ssdna Virus ◽  
Single Strain ◽  
Genome Wide ◽  
Faecal Samples ◽  
The Family ◽  
Circular Ssdna ◽  
A New Species ◽  
Dragonfly Species

Dragonfly cyclovirus (DfCyV), a new species of ssDNA virus discovered using viral metagenomics in dragonflies (family Libellulidae) from the Kingdom of Tonga. Metagenomic sequences of DfCyV were similar to viruses of the recently proposed genus Cyclovirus within the family Circoviridae. Specific PCRs resulted in the recovery of 21 DfCyV genomes from three dragonfly species (Pantala flavescens, Tholymis tillarga and Diplacodes bipunctata). The 1741 nt DfCyV genomes share >95 % nucleotide identity and are classified into 11 subtypes representing a single strain. The DfCyV genomes share 48–63 % genome-wide nucleotide identity with cycloviruses identified in human faecal samples. Recombination analysis revealed three recombinant DfCyV genomes, suggesting that recombination plays an important role in cyclovirus evolution. To our knowledge, this is the first report of a circular ssDNA virus identified in insects, and the data may help elucidate evolutionary links among novel Circoviridae recently identified in animals and environmental samples.

Molecular characterisation of a novel cassava associated circular ssDNA virus

2012 ◽  
Vol 166 (1-2) ◽  
pp. 130-135 ◽  
Author(s):  
Anisha Dayaram ◽  
Allen Opong ◽  
Anja Jäschke ◽  
James Hadfield ◽  
Marianna Baschiera ◽  
...  
Keyword(s):  
Molecular Characterisation ◽  
Ssdna Virus ◽  
Circular Ssdna

Electron Microscopy of Nucleic Acids

1974 ◽  
Vol 32 ◽  
pp. 302-303
Author(s):  
Norman Davidson
Keyword(s):  
Electron Microscopy ◽  
Nucleic Acids ◽  
Basic Protein ◽  
Molecular Biologist ◽  
Topological Properties ◽  
Protein Film ◽  
Polynucleotide Chain

The basic protein film technique for mounting nucleic acids for electron microscopy has proven to be a general and powerful tool for the working molecular biologist in characterizing different nucleic acids. It i s possible to measure molecular lengths of duplex and single-stranded DNAs and RNAs. In particular, it is thus possible to as certain whether or not the nucleic acids extracted from a particular source are or are not homogeneous in length. The topological properties of the polynucleotide chain (linear or circular, relaxed or supercoiled circles, interlocked circles, etc. ) can also be as certained.

mRNA localization by Electron microscopic in situ hybridization

1991 ◽  
Vol 49 ◽  
pp. 430-431
Author(s):  
J. A. Pollock ◽  
M. Martone ◽  
T. Deerinck ◽  
M. H. Ellisman
Keyword(s):  
Electron Microscopy ◽  
High Resolution ◽  
Nucleic Acids ◽  
Recombinant Dna ◽  
Light And Electron Microscopy ◽  
Electron Microscopic ◽  
High Voltage Electron Microscopy ◽  
Functional Sites ◽  
Voltage Electron

Localization of specific proteins in cells by both light and electron microscopy has been facilitate by the availability of antibodies that recognize unique features of these proteins. High resolution localization studies conducted over the last 25 years have allowed biologists to study the synthesis, translocation and ultimate functional sites for many important classes of proteins. Recently, recombinant DNA techniques in molecular biology have allowed the production of specific probes for localization of nucleic acids by “in situ” hybridization. The availability of these probes potentially opens a new set of questions to experimental investigation regarding the subcellular distribution of specific DNA's and RNA's. Nucleic acids have a much lower “copy number” per cell than a typical protein, ranging from one copy to perhaps several thousand. Therefore, sensitive, high resolution techniques are required. There are several reasons why Intermediate Voltage Electron Microscopy (IVEM) and High Voltage Electron Microscopy (HVEM) are most useful for localization of nucleic acids in situ.

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