scholarly journals Dragonfly cyclovirus, a novel single-stranded DNA virus discovered in dragonflies (Odonata: Anisoptera)

2011 ◽  
Vol 92 (6) ◽  
pp. 1302-1308 ◽  
Author(s):  
Karyna Rosario ◽  
Milen Marinov ◽  
Daisy Stainton ◽  
Simona Kraberger ◽  
Elizabeth J. Wiltshire ◽  
...  
Keyword(s):  
Viral Metagenomics ◽  
Nucleotide Identity ◽  
Ssdna Virus ◽  
Single Strain ◽  
Genome Wide ◽  
Faecal Samples ◽  
The Family ◽  
Circular Ssdna ◽  
A New Species ◽  
Dragonfly Species

Dragonfly cyclovirus (DfCyV), a new species of ssDNA virus discovered using viral metagenomics in dragonflies (family Libellulidae) from the Kingdom of Tonga. Metagenomic sequences of DfCyV were similar to viruses of the recently proposed genus Cyclovirus within the family Circoviridae. Specific PCRs resulted in the recovery of 21 DfCyV genomes from three dragonfly species (Pantala flavescens, Tholymis tillarga and Diplacodes bipunctata). The 1741 nt DfCyV genomes share >95 % nucleotide identity and are classified into 11 subtypes representing a single strain. The DfCyV genomes share 48–63 % genome-wide nucleotide identity with cycloviruses identified in human faecal samples. Recombination analysis revealed three recombinant DfCyV genomes, suggesting that recombination plays an important role in cyclovirus evolution. To our knowledge, this is the first report of a circular ssDNA virus identified in insects, and the data may help elucidate evolutionary links among novel Circoviridae recently identified in animals and environmental samples.

A variety of highly divergent eukaryotic ssDNA viruses in sera of pigs

2021 ◽  
Vol 102 (12) ◽  
Author(s):  
Caroline Tochetto ◽  
Samuel Paulo Cibulski ◽  
Ana Paula Muterle Varela ◽  
Cristine Cerva ◽  
Diane Alves de Lima ◽  
...  
Keyword(s):  
Potential Role ◽  
Clinical Signs ◽  
Viral Metagenomics ◽  
Dna Viruses ◽  
Circular Dna ◽  
Viral Genomes ◽  
The Family ◽  
Circular Ssdna ◽  
Ssdna Viruses ◽  
Targeted Approach

Over the last decade, viral metagenomics has been established as a non-targeted approach for identifying viruses in stock animals, including pigs. This has led to the identification of a vast diversity of small circular ssDNA viruses. The present study focuses on the investigation of eukaryotic circular Rep-encoding single-stranded (CRESS) DNA viral genomes present in serum of commercially reared pigs from southern Brazil. Several CRESS DNA viral genomes were detected, including representatives of the families Smacoviridae (n=5), Genomoviridae (n=3), Redondoviridae (n=1), Nenyaviridae (n=1) and other yet unclassified genomes (n=9), plus a circular DNA molecule, which probably belongs to the phylum Cressdnaviricota. A novel genus within the family Smacoviridae, tentatively named ‘Suismacovirus’, comprising 21 potential new species, is proposed. Although the reported genomes were recovered from pigs with clinical signs of respiratory disease, further studies should examine their potential role as pathogens. Nonetheless, these findings highlight the diversity of circular ssDNA viruses in serum of domestic pigs, expand the knowledge on CRESS DNA viruses’ genetic diversity and distribution and contribute to the global picture of the virome of commercially reared pigs.

scholarly journals Novel picornavirus (family Picornaviridae) from freshwater fishes (Perca fluviatilis, Sander lucioperca, and Ameiurus melas) in Hungary

2021 ◽  
Author(s):  
Renáta Hargitai ◽  
Péter Pankovics ◽  
Ákos Boros ◽  
Róbert Mátics ◽  
Eda Altan ◽  
...  
Keyword(s):  
Amino Acid Sequence Identity ◽  
Perca Fluviatilis ◽  
Viral Metagenomics ◽  
Rt Pcr ◽  
Sander Lucioperca ◽  
Sequence Identity ◽  
Faecal Samples ◽  
The Family ◽  
Unassigned Genus ◽  
Ameiurus Melas

AbstractIn this study, a novel picornavirus (perchPV/M9/2015/HUN, GenBank accession no. MW590713) was detected in eight (12.9%) out of 62 faecal samples collected from three (Perca fluviatilis, Sander lucioperca, and Ameiurus melas) out of 13 freshwater fish species tested and genetically characterized using viral metagenomics and RT-PCR methods. The complete genome of perchPV/M9/2015/HUN is 7,741 nt long, excluding the poly(A) tail, and has the genome organization 5’UTRIRES-?/P1(VP0-VP3-VP1)/P2(2A1NPG↓P-2A2H-box/NC-2B-2C)/P3(3A-3BVPg-3CPro-3DPol)/3’UTR-poly(A). The P1, 2C, and 3CD proteins had 41.4%, 38.1%, and 47.3% amino acid sequence identity to the corresponding proteins of Wenling lepidotrigla picornavirus (MG600079), eel picornavirus (NC_022332), and Wenling pleuronectiformes picornavirus (MG600098), respectively, as the closest relatives in the genus Potamipivirus. PerchPV/M9/2015/HUN represents a potential novel fish-origin species in an unassigned genus in the family Picornaviridae.

scholarly journals Case Report and Genomic Characterization of a Novel Porcine Nodavirus in the United States

Viruses ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 73
Author(s):  
Chenghuai Yang ◽  
Leyi Wang ◽  
Kent Schwartz ◽  
Eric Burrough ◽  
Jennifer Groeltz-Thrush ◽  
...  
Keyword(s):  
United States ◽  
Genetic Characterization ◽  
The United States ◽  
Nucleotide Identity ◽  
Flock House Virus ◽  
The Family ◽  
Genomic Characterization ◽  
A New Species ◽  
Generation Sequencing

Nodaviruses are small bisegmented RNA viruses belonging to the family Nodaviridae. Nodaviruses have been identified in different hosts, including insects, fishes, shrimps, prawns, dogs, and bats. A novel porcine nodavirus was first identified in the United States by applying next-generation sequencing on brain tissues of pigs with neurological signs, including uncontrollable shaking. RNA1 of the porcine nodavirus had the highest nucleotide identity (51.1%) to the Flock House virus, whereas its RNA2 shared the highest nucleotide identity (48%) with the RNA2 segment of caninovirus (Canine nodavirus). Genetic characterization classified porcine nodavirus as a new species under the genus Alphanodavirus. Further studies are needed to understand the pathogenicity and clinical impacts of this virus.

scholarly journals Circovirus in Blood of a Febrile Horse with Hepatitis

Viruses ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 944
Author(s):  
Alvin Hui ◽  
Eda Altan ◽  
Nathan Slovis ◽  
Caitlin Fletcher ◽  
Xutao Deng ◽  
...  
Keyword(s):  
New Species ◽  
Real Time ◽  
Genome Sequence ◽  
Real Time Pcr ◽  
Sanger Sequencing ◽  
Viral Metagenomics ◽  
Nucleotide Identity ◽  
Wide Range ◽  
Asymptomatic Infections ◽  
A New Species

Circoviruses infect vertebrates where they can result in a wide range of disease signs or in asymptomatic infections. Using viral metagenomics we analyzed a pool of five sera from four healthy and one sick horse. Sequences from parvovirus-H, equus anellovirus, and distantly related to mammalian circoviruses were recognized. PCR identified the circovirus reads as originating from a pregnant mare with fever and hepatitis. That horse’s serum was also positive by real time PCR for equine parvovirus H and negative for the flavivirus equine hepacivirus. The complete circular genome of equine circovirus 1 strain Charaf (EqCV1-Charaf) was completed using PCR and Sanger sequencing. EqCV1 replicase showed 73–74% identity to those of their closest relatives, pig circoviruses 1/2, and elk circovirus. The closest capsid proteins were from the same ungulate circoviruses with 62–63% identity. The overall nucleotide identity of 72% to its closest relative indicates that EqCV1 is a new species in the Circovirus genus, the first reported in genus Equus. Whether EqCV1 alone or in co-infections can result in disease and its prevalence in different equine populations will require further studies now facilitated using EqCV1′s genome sequence.

Coniosternum masneri sp. nov., a new species of the family Scathophagidae (Diptera) from Canada

2017 ◽  
Vol 186 (1) ◽  
pp. 113-116
Author(s):  
František Šifner
Keyword(s):  
New Species ◽  
Diagnostic Characters ◽  
The Family ◽  
A New Species

Abstract A new Nearctic species of the genus Coniosternum Becker, 1894, C. masneri sp. nov., is described from Canada, and its important diagnostic characters are illustrated.

scholarly journals Multiple Types of Novel Enteric Bopiviruses (Picornaviridae) with the Possibility of Interspecies Transmission Identified from Cloven-Hoofed Domestic Livestock (Ovine, Caprine and Bovine) in Hungary

Viruses ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 66
Author(s):  
Zoltán László ◽  
Péter Pankovics ◽  
Gábor Reuter ◽  
Attila Cságola ◽  
Ádám Bálint ◽  
...  
Keyword(s):  
Novel Species ◽  
Phylogenetic Analyses ◽  
Interspecies Transmission ◽  
Background Information ◽  
Type Ii ◽  
Rt Pcr ◽  
Faecal Samples ◽  
The Family ◽  
Domestic Livestock

Most picornaviruses of the family Picornaviridae are relatively well known, but there are certain “neglected” genera like Bopivirus, containing a single uncharacterised sequence (bopivirus A1, KM589358) with very limited background information. In this study, three novel picornaviruses provisionally called ovipi-, gopi- and bopivirus/Hun (MW298057-MW298059) from enteric samples of asymptomatic ovine, caprine and bovine respectively, were determined using RT-PCR and dye-terminator sequencing techniques. These monophyletic viruses share the same type II-like IRES, NPGP-type 2A, similar genome layout (4-3-4) and cre-localisations. Culture attempts of the study viruses, using six different cell lines, yielded no evidence of viral growth in vitro. Genomic and phylogenetic analyses show that bopivirus/Hun of bovine belongs to the species Bopivirus A, while the closely related ovine-origin ovipi- and caprine-origin gopivirus could belong to a novel species “Bopivirus B” in the genus Bopivirus. Epidemiological investigation of N = 269 faecal samples of livestock (ovine, caprine, bovine, swine and rabbit) from different farms in Hungary showed that bopiviruses were most prevalent among <12-month-old ovine, caprine and bovine, but undetectable in swine and rabbit. VP1 capsid-based phylogenetic analyses revealed the presence of multiple lineages/genotypes, including closely related ovine/caprine strains, suggesting the possibility of ovine–caprine interspecies transmission of certain bopiviruses.

A new species of the montane lacewing genus Rapisma McLachlan (Neuroptera, Ithonidae) from northwestern Yunnan, China

Zootaxa ◽  
2020 ◽  
Vol 4763 (3) ◽  
pp. 439-443
Author(s):  
XINGYUE LIU
Keyword(s):  
New Species ◽  
Southeast Asia ◽  
East Asia ◽  
South Asia ◽  
The Family ◽  
A New Species ◽  
Northwestern Yunnan

The genus Rapisma McLachlan, 1866 (montane lacewings) is a rare and little known group of the family Ithonidae (Insecta: Neuroptera). There have been 21 described species of Rapisma, and all of them are distributed from East Asia, South Asia and Southeast Asia. Here I report a new species of Rapisma from northwestern Yunnan, China, namely Rapisma weixiense sp. nov. The new species belongs to a group of Rapisma species with very short antennae. 

Description of a new species of Auriculostoma (Digenea: Allocreadiidae) from Characidium heirmostigmata (Characiformes: Crenuchidae) from Argentina, using morphological and molecular data

2021 ◽  
Vol 95 ◽  
Author(s):  
M.M. Montes ◽  
J. Barneche ◽  
Y. Croci ◽  
D. Balcazar ◽  
A. Almirón ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
National Park ◽  
Molecular Data ◽  
Rrna Gene ◽  
28S Rrna ◽  
The Family ◽  
Parasitological Survey ◽  
Morphological And Molecular Data ◽  
A New Species

Abstract During a parasitological survey of fishes at Iguazu National Park, Argentina, specimens belonging to the allocreadiid genus Auriculostoma were collected from the intestine of Characidium heirmostigmata. The erection of the new species is based on a unique combination of morphological traits as well as on phylogenetic analysis. Auriculostoma guacurarii n. sp. resembles four congeneric species – Auriculostoma diagonale, Auriculostoma platense, Auriculostoma tica and Auriculostoma totonacapanensis – in having smooth and oblique testes, but can be distinguished by a combination of several morphological features, hosts association and geographic distribution. Morphologically, the new species can be distinguished from both A. diagonale and A. platense by the egg size (bigger in the first and smaller in the last); from A. tica by a shorter body length, the genital pore position and the extension of the caeca; and from A. totonacapanensis by the size of the oral and ventral sucker and the post-testicular space. Additionally, one specimen of Auriculostoma cf. stenopteri from the characid Charax stenopterus (Characiformes) from La Plata River, Argentina, was sampled and the partial 28S rRNA gene was sequenced. The phylogenetic analysis revealed that A. guacurarii n. sp. clustered with A. tica and these two as sister taxa to A. cf. stenopteri. The new species described herein is the tenth species in the genus and the first one parasitizing a member of the family Crenuchidae.

A new species and further records of quill mites (Acari: Cheyletoidea: Syringophilidae) parasitic on birds (Aves) in England

Zootaxa ◽  
2009 ◽  
Vol 2133 (1) ◽  
pp. 49-54 ◽  
Author(s):  
BARRY NATTRESS ◽  
MACIEJ SKORACKI
Keyword(s):  
New Species ◽  
Length Ratio ◽  
Additional Species ◽  
Tawny Owl ◽  
Strix Aluco ◽  
Quill Mites ◽  
The Family ◽  
Transverse Branch ◽  
One New Species ◽  
A New Species

Four additional species of quill mites of the family Syringophilidae Lavoipierre have now been recorded in England. This includes one new species, Bubophilus aluconis sp. nov., which parasitizes the tawny owl Strix aluco (Strigiformes: Strigidae). It differs from other species of this genus, B. ascalaphus Philips et Norton, 1978 and B. asiobius Skoracki et Bochkov, 2002 by the number of chambers in transverse branch of the peritremes (2-3), the length ratio of setae vi and ve (1:1.6-2), and the lengths of the stylophore and aggenital setae ag1 (180 and 135-145, respectively).

Cryptofavognathus, a new genus of the family Cryptognathidae Oudemans (Acari: Raphignathoidea), with the description of a new species from Turkey

Zootaxa ◽  
2010 ◽  
Vol 2533 (1) ◽  
pp. 36 ◽  
Author(s):  
SALİH DOĞAN ◽  
GÜLDEM DÖNEL
Keyword(s):  
New Species ◽  
Adult Female ◽  
New Genus ◽  
The Family ◽  
A New Species

A new genus, Cryptofavognathus is proposed for two species, Cryptofavognathus afyonensis (Koç & Akyol, 2004) comb. nov. and C. anatolicus sp. nov. The adult female and male of C. anatolicus sp. nov. collected from moss and a bird’s nest are herein described and figured.

Sign in / Sign up

Export Citation Format

Share Document