scholarly journals Chronic stress differentially affects antioxidant enzymes and modifies the acute stress response in liver of Wistar rats

2010 ◽  
pp. 729-736
Author(s):  
J Djordjevic ◽  
A Djordjevic ◽  
M Adzic ◽  
A Niciforovic ◽  
MB Radojcic
Keyword(s):  
Antioxidant Enzymes ◽  
Protein Expression ◽  
Chronic Stress ◽  
Wistar Rats ◽  
Acute Stress ◽  
Long Duration ◽  
Male Wistar Rats ◽  
Expression Activity ◽  
Acute Stress Response ◽  
Upstream Regulators

Clinical reports suggest close interactions between stressors, particularly those of long duration, and liver diseases, such as hepatic inflammation, that is proposed to occur via reactive oxygen species. In the present study we have used 21-day social isolation of male Wistar rats as a model of chronic stress to investigate protein expression/activity of liver antioxidant enzymes: superoxide dismutases (SODs), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GLR), and protein expression of their upstream regulators: glucocorticoid receptor (GR) and nuclear factor kappa B (NFkB). We have also characterized these parameters in either naive or chronically stressed animals that were challenged by 30-min acute immobilization. We found that chronic isolation caused decrease in serum corticosterone (CORT) and blood glucose (GLU), increase in NFkB signaling, and disproportion between CuZnSOD, peroxidases (CAT, GPx) and GLR, thus promoting H2O2 accumulation and prooxidative state in liver. The overall results suggested that chronic stress exaggerated responsiveness to subsequent stressor at the level of CORT and GLU, and potentiated GLR response, but compromised the restoration of oxido-reductive balance due to irreversible alterations in MnSOD and GPx.

P-OT002. Alcohol consumption alters the amygdalar protein expression of mTORC2 in male Wistar rats

2021 ◽  
Vol 132 (8) ◽  
pp. e123
Author(s):  
Athirah Hanim Binti Che Rahim ◽  
Mohd Helmy Mokhtar ◽  
Rashidi Mohamed Pakri ◽  
Isa Naina Mohamed ◽  
Jayakumar
Keyword(s):  
Alcohol Consumption ◽  
Protein Expression ◽  
Wistar Rats ◽  
Male Wistar Rats

scholarly journals Effect of different types of stress on adrenal gland parameters and adrenal hormones in the blood serum of male Wistar rats

2009 ◽  
Vol 61 (2) ◽  
pp. 187-194 ◽  
Author(s):  
M. Adzic ◽  
Ana Djordjevic ◽  
Jelena Djordjevic ◽  
Ana Niciforovic ◽  
Marija Radojcic
Keyword(s):  
Blood Serum ◽  
Wistar Rats ◽  
Adrenal Glands ◽  
Acute Stress ◽  
Serum Levels ◽  
Combined Stress ◽  
Adrenal Hormones ◽  
General Adaptation ◽  
Different Types ◽  
Male Wistar Rats

In the present study, we examined gross changes in the mass of whole adrenal glands and that of the adrenal cortex and medulla in mature male Wistar rats subjected to three different stress types: acute, chronic, and combined, i.e., chronic followed by acute stress. These parameters were correlated with adrenal activity as judged from serum levels of corticosterone and catecholamine, respectively, as well as with serum levels of ACTH and glucose. Under all three conditions, we observed bilaterally asymmetric and stress-type-independent hypertrophy of whole adrenals, as well as adrenal cortices and medullas. Under acute and combined stress, adrenal hypertrophy was followed by increase of adrenal hormones in the blood serum. However, under chronic stress, both cortical and medullar activities as judged from low or unaltered levels of the respective hormones and glucose were compromised and disconnected from the input signal of ACTH. Since all of the studied adrenal activities could be restored by subsequent acute stress, it is concluded that chronic isolation can be viewed as partly maladaptive stress with characteristics resembling stress resistance rather than the stress exhaustion stage of the general adaptation syndrome.

scholarly journals The Association of Malnutrition and Chronic Stress Models Does Not Present Overlay Effects in Male Wistar Rats

2016 ◽  
Vol 4 ◽  
Author(s):  
Camila Gracyelle de Carvalho Lemes ◽  
Abraão Tiago Batista Guimarães ◽  
Wellington Alves Mizael da Silva ◽  
Bruna de Oliveira Mendes ◽  
Dieferson da Costa Estrela ◽  
...  
Keyword(s):  
Chronic Stress ◽  
Wistar Rats ◽  
Male Wistar Rats ◽  
Stress Models

scholarly journals Effects of chronic stress and high-fat diet on metabolic and nutritional parameters in Wistar rats

2013 ◽  
Vol 57 (8) ◽  
pp. 642-649 ◽  
Author(s):  
Thiago Bruder-Nascimento ◽  
Dijon Henrique Salomé Campos ◽  
Carlos Alves ◽  
Samuel Thomaz ◽  
Antônio Carlos Cicogna ◽  
...  
Keyword(s):  
Chronic Stress ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Normal Diet ◽  
High Fat ◽  
Final Body Weight ◽  
Insulin Resistant ◽  
Male Wistar Rats ◽  
Total Fat ◽  
Level Of Significance

OBJECTIVE: The aim of this study was assess the role of chronic stress on the metabolic and nutritional profile of rats exposed to a high-fat diet. MATERIALS AND METHODS: Thirty-day-old male Wistar rats (70-100 g) were distributed into four groups: normal-diet (NC), chronic stress (St), high-fat diet (HD), and chronic stress/high-fat diet (HD/St). Stress consisted at immobilization during 15 weeks, 5 times per week, 1h per day; and exposure to the high-fat diet lasted 15 weeks. Nutritional and metabolic parameters were assessed. The level of significance was 5%. RESULTS: The HD group had final body weight, total fat, as well as insulin and leptin increased, and they were insulin resistant. The St and HD/St had arterial hypertension and increased levels of corticosterone. Stress blocked the effects of the high-fat diet. CONCLUSION: Chronic stress prevented the appearance of obesity. Our results help to clarify the mechanisms involved in metabolic and nutritional dysfunction, and contribute to clinical cases linked to stress and high-fat diet.

Gastroprotective activity of essential oils from turmeric and ginger

2015 ◽  
Vol 26 (1) ◽  
Author(s):  
Vijayasteltar B. Liju ◽  
Kottarapat Jeena ◽  
Ramadasan Kuttan
Keyword(s):  
Gastric Ulcer ◽  
Antioxidant Enzymes ◽  
Essential Oils ◽  
Wistar Rats ◽  
Histopathological Examination ◽  
Antiulcer Activity ◽  
Ulcer Index ◽  
Degree Of Protection ◽  
Male Wistar Rats ◽  
Different Doses

AbstractTurmeric (Turmeric and ginger were evaluated for their antiulcer activity against ethanol-induced ulcers in male Wistar rats at different doses: 100, 500 and 1000 mg/kg body weight. Ethanol was used to induce gastric ulcer in Wistar rats. Parameters such as ulcer index, histopathology and levels of antioxidant enzymes such as glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase and glutathione (GSH) levels were measured to assess the degree of protection produced by the essential oils.TEO and GEO inhibited ulcer by 84.7% and 85.1%, respectively, as seen from the ulcer index. Reduced antioxidant enzymes such as GPx, SOD, catalase and GSH produced by alcohol administration were significantly (p<0.001) increased by simultaneous administration of TEO and GEO. Histopathological examination showed that ethanol-induced lesions such as necrosis, erosion and hemorrhage of the stomach wall were significantly reduced after oral administration of essential oils.Results suggest that TEO and GEO could reduce the gastric ulcer in rat stomach as seen from the ulcer index and histopathology of the stomach. Moreover, oxidative stress produced by ethanol was found to be significantly reduced by TEO and GEO.

Antioxidant and nephroprotective potential of butylated hydroxyanisole against ferric nitrilotriacetate-induced oxidative stress and early tumor events

2015 ◽  
Vol 35 (4) ◽  
pp. 448-453 ◽  
Author(s):  
S Ansar ◽  
M Iqbal
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Wistar Rats ◽  
Thymidine Incorporation ◽  
Albino Rats ◽  
Butylated Hydroxyanisole ◽  
Ferric Nitrilotriacetate ◽  
Wistar Strain ◽  
Male Wistar Rats ◽  
Early Tumor

The present study was aimed to study protective effect of butylated hydroxyanisole (BHA), a phenolic antioxidant used in foods on ferric nitrilotriacetate (Fe-NTA)–induced nephrotoxicity. Male albino rats of Wistar strain (4–6 weeks old) weighing 125–150 g were used in this study. Animals were given a single dose of Fe-NTA (9 mg kg−1 body weight) after treatment with BHA (1 and 2 mg animal−1 day−1). Fe-NTA treatment enhanced ornithine decarboxylase (ODC) activity to 5.3-fold, and [3H]-thymidine incorporation in DNA to 2.5-fold in kidney compared with the corresponding saline-treated control, whereas glutathione (GSH) levels and the activities of antioxidant enzymes decreased to a range of 2- to 2.5-fold in kidney. These changes were reversed significantly in animals receiving a pretreatment of BHA. The enhanced ODC activity and DNA synthesis showed a reduction to 2.12-fold and 1.15-fold, respectively, at a higher dose of 2 mg BHA day−1 animal−1, compared with the Fe-NTA-treated groups. Pretreatment with BHA prior to Fe-NTA treatment increased GSH and the activities of antioxidant enzymes to a range of 1.5- to 2-fold in kidney. The results indicate that BHA suppresses Fe-NTA-induced nephrotoxicity in male Wistar rats.

Enhanced Na+, K+-ATPase activity and endothelial modulation decrease phenylephrine-induced contraction in aorta from ouabain-treated normotensive and hypertensive rats

2014 ◽  
Vol 18 (2) ◽  
Author(s):  
Ana Paula Davel ◽  
Gisele Kruger Couto ◽  
Camilla Ferreira Wenceslau ◽  
Emilia Cristina Peres ◽  
Fabiano Elias Xavier ◽  
...  
Keyword(s):  
Protein Expression ◽  
Wistar Rats ◽  
Vascular Reactivity ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Vasoconstrictor Response ◽  
Adrenergic Response ◽  
Male Wistar Rats ◽  
Cox 2

AbstractThe purpose of this study was to compare the effect of long-term ouabain treatment on the vascular reactivity and NaMale Wistar rats were treated with ouabain (~8.0 µg/day, subcutaneously) or vehicle for 5 and 20 weeks, and spontaneously hypertensive rats (SHRs) for 5 weeks. Vasoconstrictor response to phenylephrine (10The phenylephrine-induced contraction was reduced, whereas the relaxation to KCl was enhanced in the aorta of ouabain-treated Wistar rats and SHRs. In both strains, endothelial modulation of α-adrenergic response was enhanced, related to an increased NO and reduced COX-derived vasoconstrictor factor modulation. Aortas from 20-week ouabain-treated Wistar rats showed reduced COX-2 and enhanced eNOS protein expression. In SHRs, 5-week ouabain treatment reduced COX-2 and increased nNOS protein expression.The results suggest that long-term ouabain treatment reduces the α-adrenergic response of aorta from normotensive rats and SHRs, associated with an increase of NO synthesis, reduced COX-2-derived vasoconstrictor factors, and enhanced ouabain-sensitive Na

scholarly journals Centella asiatica ethanol extract increases hippocampal brain derived neurotrophic factor in male Wistar rats

2018 ◽  
Vol 37 (2) ◽  
pp. 143
Author(s):  
Astri Handayani ◽  
Sophie Yolanda ◽  
Ria Kodariah
Keyword(s):  
Synaptic Plasticity ◽  
Protein Expression ◽  
Adult Male ◽  
Wistar Rats ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
Memory Formation ◽  
Male Rats ◽  
Hippocampal Region ◽  
Male Wistar Rats

Background<br />Synaptic plasticity, which primarily takes place in the hippocampus, is the molecular basis of long- term memory formation. Brain derived neurotrophic factor (BDNF), a member of the neurotrophin family, plays a significant role in synaptic plasticity and memory formation. When BDNF is released, it binds to its receptor and activates various intracellular signal transduction pathways leading to synaptic plasticity. Several methods to improve memory function in humans have been studied, one of which is the use of herbal compounds, such as Centella asiatica (CeA), an herbaceous plant that has been used for improving memory. This study aims to examine the effects of CeA ethanol extract on BDNF protein expression in the CA1 hippocampal region in adult male rats.<br /><br />Methods<br />A randomized experimental design was performed involving 18 adult male Wistar rats. The rats were randomized into three groups: one control/distilled water group and two groups treated with doses of CeA ethanol extract of 300 mg/kgBW (CeA300) and 600 mg/kgBW (CeA600), respectively. CeA ethanol extract was administered orally for 28 consecutive days with weekly weight-adjusted dose. After 28 days, the rats were decapitated, and the hippocampus was isolated from the brain. BDNF protein expression was assessed using immunohistochemistry. Data was analyzed using Kruskal-Wallis test and continued with post-hoc analysis. <br /><br />Results<br />There was a significant increase in BDNF protein expression in the CeA600 group compared to the control group (p&lt;0.001). <br /><br />Conclusion<br />Administration of CeA ethanol extract increased BDNF protein expression in the CA1 hippocampal region of adult male rats.

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