ADAPTOGENIC EFFECT OF APISIL AND TOS-BIO PREPARATIONS ON A HONEY BEE UNDER CONDITIONS OF HYPERTHERMIA

The temperature factor has a huge impact on bees and their development. In a complex complex of adaptation, not only a perfect system of collective thermoregulation of the family is important, but also such individual adaptations as resistance to freezing and tolerance to high temperatures. The deviation of the ambient temperature from the optimal one increases the energy consumption of bees for the regulation of the microclimate in the family. This means that it enhances metabolic processes, which affects the state of the body and the life expectancy of insects. In addition, sometimes the use of high temperatures is a necessary condition for their maintenance, for example, in the fight against the parasite of the bee mite varroa. At the same time, the effect of high temperatures can have a negative effect not only on the mite, but also on the bees. In laboratory conditions, the effect of Apisil and TOS-BIO preparations containing 9-oxoand 10-hydroxy-2E-decenoic acids as a base - the most important components of the «queen substance» and royal jelly of the honey bee Apis mellifera L., respectively - on the survival rate and biochemical parameters was studied. adult worker bees exposed to extremely high (50oC) temperatures. The dynamics of the activity of phenoloxidase and catalase as biochemical indicators of the stress response of a honey bee under high temperature load has been studied. It was shown that the insects' resistance to hyperthermia increased with the content of these preparations in the feed. Experimental results indicate that under extreme (50oC) temperature conditions Apisil and TOS-BIO preparations have an adaptogenic effect on worker bees, increasing their resistance to hyperthermia. At the same time, changes in the initial level and dynamics of biochemical parameters indicate that these compositions in the most economical way contribute to the restructuring of metabolism in the mode of optimal functioning, which affects the level of changes in enzyme activity (with less pronounced changes in activity). and also on the survival rate of bees at the end of the experiment.

ASSESSMENT OF RADIO PROTECTIVE AND ADAPTGENIC ACTIONS OF THE EXPERIMENTAL PREPARATION

In the course of the research, the radioprotective and adaptogenic effects of the new feed additive were determined in experiments on white mice and white rats. It was found that the inclusion of the studied feed additive in the diet of mice after irradiation provided an increase in the survival rate of animals irradiated with a lethal dose by 50% compared to the control. Feeding the mice with the drug followed by irradiation with a lethal dose increased the survival rate by 42 %. In a similar experiment on white rats, the survival rate of the experimental animals was higher than the control values by 58 and 50 %, respectively. When assessing the adaptogenic effect of the drug, it was found that 12-hour immobilization of rats led to significant changes in the prooxidant-antioxidant defense system. Preliminary feeding of the studied feed additive had a protective effect, which was manifested in a lower content of malondialdehyde in the blood serum of experimental animals, compared with control animals, by 28.0 %, an increased content of catalase by 11.1 %, superoxide dismutase by 43.9 %, reduced glutathione - by 17.6 %. Thus, the investigated drug showed a well-pronounced radioprotective and adaptogenic effect.

PHYSIOLOGICAL AND BIOCHEMICAL EVALUATION OF THE EFFECTIVENESS OF A NEW FOOD INGREDIENT: A SOURCE OF PHYTOECDYSTEROIDS AND FLAVONOIDS FROM QUINOA GRAIN

A new food ingredient has been developed - a source of phytoecdysteroids and flavonoids from quinoa grain. A physiological and biochemical assessment of its effectiveness under conditions of chronic immobilization stress and a single e exhausting physical activity was carried out. A pronounced adaptogenic effect of the developed food ingredient was shown.

FEEDING OF SPORTING DOGS PART II. MINERALS, VITAMINS AND FUNCTIONAL ADDITIVES REQUIREMENTS

Sporting dog owner should realize that proper nutrition is one of the most important factors influencing the sports results achieved by the dog. In addition to the increased demand for energy and basic nutrients, particular attention should be paid to the adequate supply of vitamins and minerals, as well as dietary supplements. Therefore, the aim of the study was to characterize the importance of minerals, vitamins and functional additives in the nutrition of sporting dogs. The significant elements in the sporting dogs nutrition are calcium, phosphorus, potassium, sodium and magnesium. Micronutrients such as copper, iron, manganese, selenium and zinc are also very important. Of the vitamins in the sporting dogs nutrition, vitamins with antioxidant properties (vitamin E, vitamin C) are crucial, as well as vitamin D and B-group vitamins. Moreover, the diet of a sporting dog can also be enriched with functional additives that support the function of joints, muscles and circulatory system, as well as plants with an adaptogenic effect that improve the dog's immunity. Selecting the right amounts of individual additives so they fulfill their planned functions and are completely safe may be a problem. Since it should be remembered that both the deficiency and the excess of nutrients can be dangerous for the body.

Assessment of the effectiveness of physiotherapeutic methods for management of early menopause disorders

Objective. Assessment of the effectiveness of pelotherapy in combination with phytoaromatic baths in women during menopause transition. Patients and methods. We examined 26 women aged 52 ± 2.4 years. All women with vegetative-vascular manifestations of the climacteric syndrome were randomly assigned to 2 groups for comparison: group 1 – 12 patients, who received full-body “rapa” baths, inhalation phytoaromatherapy with a complex composition including phytoestrogens; group 2 – 14 patients, who received pelotherapy with applications of Saki lake mud on the «panty» zone and hydrodynamic phytoaromatic baths with a complex composition including phytoestrogens. Results. After therapy, a significant improvement of the parameters of climacteric disorders was noted in group 2. Severe disorders were practically absent in the second group – 2% (65 ± 6.1 scores), whereas mild changes prevailed – 64% (18 ± 5.3 scores). Changes in the microcirculation parameter were found, which increased by 19%, an increased contribution of endothelial oscillations to the total flux motion level, an increase of the amplitude of neurogenic and myogenic oscillations, a decrease of respiratory rates on average from 9.2 to 5.51 perf. units. In the first group, no significant changes were recorded. Conclusion. The use of pelotherapy in combination with hydrodynamic phytoaromatic baths permits to improve the parameters of microcirculation, venous and lymph output, trophism and oxygenation of tissues, and also ensures an adaptogenic effect. Key words: balneothaerpy, menopause transition, pelotherapy, rehabilitation

Adaptogenic effect of phlojodicarpus sibiricus extract on cerebral ischemia

The aim of the study was to determine the adaptogenic effect of the extract from the roots of Phlojodicarpus sibiricus during ischemic influence on the left hemisphere of the brain. Experiments were conducted on Wistar rats of both sexes weighing 200-220 g. Unilateral cerebral ischemia was induced by permanent occlusion of the left common carotid artery under ether anesthesia. Adaptogenic anti-ischemic effect of Ph. sibiricus was determined by the number of desquamated endotheliocytes, nitrogen oxide metabolites – nitrites in the blood, by the content of lipid peroxidation products – malondialdehyde (MDA) and the activity of the antioxidant defense system in the brain tissue. It is established that Ph. sibiricus has an anti-ischemic effect, reduces the number of endotheliocytes desquamated, increases the level of nitric oxide in the blood (p ≤ 0.05), stimulates SOD and catalase activity, reducing the intensity of lipid peroxidation and the accumulation of MDA in the brain during ischemic exposure (p ≤ 0.05). Preventive administration of Ph. sibiricus extract has an adaptogenic effect in unilateral cerebral ischemia, which consists in reducing desquamated endotheliocytes, increasing the production of nitric oxide by endothelium, increasing the antioxidant defense system and reducing the intensity of oxidative reactions in the brain. Adaptogenic effect of Ph. sibiricus extract is caused by endothelium protective and antioxidant effects.