scholarly journals Effect of Short- and Long-Term High-Fat Feeding on Autophagy Flux and Lysosomal Activity in Rat Liver

2012 ◽  
pp. S67-S76 ◽  
Author(s):  
Z. PAPÁČKOVÁ ◽  
H. DAŇKOVÁ ◽  
E. PÁLENÍČKOVÁ ◽  
L. KAZDOVÁ ◽  
M. CAHOVÁ

Autophagy-lysosomal pathway is a cellular mechanism ensuring degradation of various macromolecules like proteins or triacylglycerols (TAG). Its disruption is related to many pathological states, including liver steatosis. We compared the effect of short- and long-established steatosis on the intensity of autophagy-lysosomal pathway in rat liver. The experiments were carried out on 3-month old Wistar rats fed standard (SD) or high-fat diet for 2 (HF-2) or 10 (HF-10) weeks. HF diet administered animals accumulated an increased amount of TAG in the liver (HF-2→HF-10). Autophagy flux was up-regulated in HF-2 group but nearly inhibited after 10 weeks of HF administration. The expression of autophagy related genes was up-regulated in HF-2 but normal in HF-10. In contrast, total activities of two lysosomal enzymes, lysosomal lipase (LAL) and acid phosphatase, were unaffected in HF-2 but significantly increased in HF-10 groups. mRNA expression of lysosomal enzymes was not affected by the diet. We conclude that in a state of metabolic unbalance (steatosis), autophagy machinery and lysosomal enzymes expression are regulated independently. The accumulation of TAG in the liver is associated with the increase of total LAL activity and protein expression. In contrast, the autophagy response is bi-phasic and after rapid increase it is significantly diminished. This may represent an adaptive mechanism that counteracts the excessive degradation of substrate, i.e. TAG, and eliminate over-production of potentially hazardous lipid-degradation intermediates.

Hepatology ◽  
2009 ◽  
Vol 51 (6) ◽  
pp. 2234-2235 ◽  
Author(s):  
Adriana L. Burgueño ◽  
Julieta Carabelli ◽  
Silvia Sookoian ◽  
Carlos J. Pirola

Author(s):  
Jolita Ciapaite ◽  
Nicole M. van den Broek ◽  
Heleen te Brinke ◽  
Klaas Nicolay ◽  
Jeroen A. Jeneson ◽  
...  

1995 ◽  
Vol 19 (3) ◽  
pp. 155-163
Author(s):  
Taro MURAKAMI ◽  
Yoshiharu SHIMOMURA ◽  
Tomohiro TAMURA ◽  
Kotoyo FUJIKI ◽  
Yoshihisa SHIMIZU ◽  
...  

PLoS ONE ◽  
2016 ◽  
Vol 11 (9) ◽  
pp. e0163883 ◽  
Author(s):  
Catrina Sims-Robinson ◽  
Anna Bakeman ◽  
Elizabeth Bruno ◽  
Samuel Jackson ◽  
Rebecca Glasser ◽  
...  

In Vivo ◽  
2018 ◽  
Vol 32 (4) ◽  
pp. 813-817
Author(s):  
OZLEM TIMIRCI-KAHRAMAN ◽  
UMIT YILMAZ ◽  
NESIBE YILMAZ ◽  
AYDIN CEVIK ◽  
CEM HOROZOGLU ◽  
...  

2018 ◽  
Vol 507 (1-4) ◽  
pp. 291-296 ◽  
Author(s):  
Saki Yoshimura ◽  
Shihoko Nakashima ◽  
Yuki Tomiga ◽  
Shotaro Kawakami ◽  
Yoshinari Uehara ◽  
...  

2000 ◽  
Vol 84 (3) ◽  
pp. 377-385 ◽  
Author(s):  
Susanna Iossa ◽  
Lillà Lionetti ◽  
Maria P. Mollica ◽  
Raffaella Crescenzo ◽  
Antonio Barletta ◽  
...  

The purpose of this work was to study the effect of early long-term high-fat feeding on energy balance and liver oxidative activity. To this end, rats aged about 30 d were fed a high-fat or a low-fat diet for 15, 30 or 60 d. Full energy balance and energy partitioning measurements were carried out. In addition, we measured hepatic mitochondrial and peroxisomal oxidative capacities. Serum levels of free triiodothyronine (T3) and leptin were also determined. Rats fed a high-fat diet showed an increase in metabolizable energy intake as well as in energy expenditure, while lipid gain over the whole period was lower than that expected due to a decrease in metabolic efficiency. An increase in serum free T3 levels was also found in rats fed a high-fat diet after 15 and 30 d. Statistically significant correlations between serum leptin levels and body fat mass were found after 15, 30 and 60 d of high-fat feeding. Finally, no variation in hepatic mitochondrial and peroxisomal fatty acid oxidation capacity was found in rats fed a high-fat diet for 15, 30 or 60 d. In conclusion, the results of the present study show that young Wistar rats fed a high-fat diet for up to 60 d are able to counteract, at least in part, obesity development.


2009 ◽  
Vol 54 (1) ◽  
Author(s):  
M Wierzbicki ◽  
A Chabowski ◽  
M Żendzian-Piotrowska ◽  
E Harasim ◽  
J Górski
Keyword(s):  
High Fat ◽  

2021 ◽  
Author(s):  
Qi Guan ◽  
Xinwen Ding ◽  
Lingyue Zhong ◽  
Chuang Zhu ◽  
Pan Nie ◽  
...  

Long term high-fat diet (HF) can cause metabolic disorders, which might induce fatty liver. Fermented whole cereal food exhibit healthy potential due to their unique phytochemical composition and probiotics. In...


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