Synthesis of Linoleic Acid 13-Hydroperoxides from Safflower Oil Utilizing Lipoxygenase in a Coupled Enzyme System with In-Situ Oxygen Generation

Linoleic acid hydroperoxides are versatile intermediates for the production of green note aroma compounds and bifunctional ω-oxo-acids. An enzyme cascade consisting of lipoxygenase, lipase and catalase was developed for one-pot synthesis of 13-hydroperoxyoctadecadienoic acid starting from safflower oil. Reaction conditions were optimized for hydroperoxidation using lipoxygenase 1 from Glycine max (LOX-1) in a solvent-free system. The addition of green surfactant Triton CG-110 improved the reaction more than two-fold and yields of >50% were obtained at linoleic acid concentrations up to 100 mM. To combine hydroperoxidation and oil hydrolysis, 12 lipases were screened for safflower oil hydrolysis under the reaction conditions optimized for LOX-1. Lipases from Candida rugosa and Pseudomonas fluorescens were able to hydrolyze safflower oil to >75% within 5 h at a pH of 8.0. In contrast to C. rugosa lipase, the enzyme from P. fluorescens did not exhibit a lag phase. Combination of P. fluorescens lipase and LOX-1 worked well upon LOX-1 dosage and a synergistic effect was observed leading to >80% of hydroperoxides. Catalase from Micrococcus lysodeikticus was used for in-situ oxygen production with continuous H2O2 dosage in the LOX-1/lipase reaction system. Foam generation was significantly reduced in the 3-enzyme cascade in comparison to the aerated reaction system. Safflower oil concentration was increased up to 300 mM linoleic acid equivalent and 13-hydroperoxides could be produced in a yield of 70 g/L and a regioselectivity of 90% within 7 h.

The Balancing of Safflower Oil and Inositol to Intestinal Morphometric of Sentul Chicken

This study was aimed to evaluate the use of safflower oil (Carthamus tinctorius L) and inositol on the digestive profile of male Sentul chickens. A total of 182 Sentul chickens aged 17 weeks were reared up to 23 weeks in 91 units of battery cage (6 chickens/unit). The research was conducted in a Completely Randomized Design (CRD) with nine treatments and three replicates (6 chickens/replicate). The research treatments were R0 = control/ basal feed + 0% Safflower and 0% Inositol; R1 = Basal feed + 0.5% Safflower oil; R2 = Basal feed + 1.0% Safflower oil; R3 = Basal feed + 0.5% Inositol; R4 = Basal feed + 1.0% Inositol; R5 = Basal feed + 0.5% Safflower oil and 0.5% Inositol; R6 = Basal feed + 0.5% Safflower oil and 1.0% Inositol; R7 = Basal feed + 1.0% Safflower oil and 0.5% Inositol; R8 = Basal feed + 1.0% Safflower oil and 1.0% Inositol. Basal feed was composed of corn, rice bran, soybean kernel, fishmeal, palm oil, calcium carbonate (CaCO3), topmix, lysine, and methionine, as well as safflower oil (Carthamus tinctorius L) and inositol. The measured variable consists of digestive profile (the percentage of intestine weight, digesta, proventriculus, gizzard) and intestine length, crypt depth, the width and length of intestinal villi, Intestinal histology profile. The data were subjected to a statistical analysis of variance (ANOVA) continued with an Honestly Significant Difference test (HSD). The result showed that incorporating safflower oil (Carthamus tinctorius L) and inositol into feed did not significantly affect (P>0.05) the digestive profile of Male Sentul chickens. Conclusively, safflower oil and inositol up to 1% in the feed are safe for male Sentul chickens without interfering with the performance of digestive organs because it produces relatively similar intestinal weight and length, crypt depth, as well as the length and width of intestinal villi.