scholarly journals Cellular Physiology and Biochemistry

2021 ◽  
2019 ◽  
Author(s):  
Wenfa Ng

Multiple biological mysteries remain in the definition and organization of genetic information into different chromosomes. Up to now, genome architecture at the chromosome level remain enigmatic concerning the reasons why evolution and natural selection arranged genetic information in separate segments in eukaryotic cells as compared to the single chromosome in the prokaryotic world. Specifically, one important unresolved question has been the role of chromosomes in cellular physiology and biochemical processes. By deleting the centromere and telomere regions of different chromosomes in Saccharomyces cerevisiae and fusing the different chromosomes into one chromosome, research reported by Shao and coworkers in Nature revealed the technical possibility of concatenating all genetic information into one segment. Furthermore, cell viability assays revealed that there was no significant loss of cell viability after the fusing of 16 chromosomes into a single chromosome. This highlighted that centromere and telomere sequences were not critical to overall cellular function, physiology and biochemistry. More importantly, the results highlighted that genetic information and its organisation at the sub-chromosome level play a more important role in defining cellular biochemical processes and physiology such as metabolism and cell division processes. Collectively, the technical feasibility of fusing multiple chromosomes into a single chromosome has been shown in new research that deleted the centromere and telomere regions of different chromosomes for fusing the resulting genetic information into a single chromosome. Little loss of viability and function in cells with a single chromosome and the stability of replicating the chromosome revealed that centromere and telomere sequences may not play critical roles in defining cellular physiology and biochemistry. More importantly, genomic information and its regulation was shown indirectly to have a more direct influence on cell physiology and metabolism than chromosomal architecture.


2019 ◽  
Author(s):  
Wenfa Ng

Multiple biological mysteries remain in the definition and organization of genetic information into different chromosomes. Up to now, genome architecture at the chromosome level remain enigmatic concerning the reasons why evolution and natural selection arranged genetic information in separate segments in eukaryotic cells as compared to the single chromosome in the prokaryotic world. Specifically, one important unresolved question has been the role of chromosomes in cellular physiology and biochemical processes. By deleting the centromere and telomere regions of different chromosomes in Saccharomyces cerevisiae and fusing the different chromosomes into one chromosome, research reported by Shao and coworkers in Nature revealed the technical possibility of concatenating all genetic information into one segment. Furthermore, cell viability assays revealed that there was no significant loss of cell viability after the fusing of 16 chromosomes into a single chromosome. This highlighted that centromere and telomere sequences were not critical to overall cellular function, physiology and biochemistry. More importantly, the results highlighted that genetic information and its organisation at the sub-chromosome level play a more important role in defining cellular biochemical processes and physiology such as metabolism and cell division processes. Collectively, the technical feasibility of fusing multiple chromosomes into a single chromosome has been shown in new research that deleted the centromere and telomere regions of different chromosomes for fusing the resulting genetic information into a single chromosome. Little loss of viability and function in cells with a single chromosome and the stability of replicating the chromosome revealed that centromere and telomere sequences may not play critical roles in defining cellular physiology and biochemistry. More importantly, genomic information and its regulation was shown indirectly to have a more direct influence on cell physiology and metabolism than chromosomal architecture.


Author(s):  
William W. Thomson ◽  
Elizabeth S. Swanson

The oxidant air pollutants, ozone and peroxyacetyl nitrate, are produced in the atmosphere through the interaction of light with nitrogen oxides and gaseous hydrocarbons. These oxidants are phytotoxicants and are known to deleteriously affect plant growth, physiology, and biochemistry. In many instances they induce changes which lead to the death of cells, tissues, organs, and frequently the entire plant. The most obvious damage and biochemical changes are generally observed with leaves.Electron microscopic examination of leaves from bean (Phaseolus vulgaris L.) tobacco (Nicotiana tabacum L.) and cotton (Gossipyum hirsutum L.) fumigated for .5 to 2 hours with 0.3 -1 ppm of the individual oxidants revealed that changes in the ultrastructure of the cells occurred in a sequential fashion with time following the fumigation period. Although occasional cells showed severe damage immediately after fumigation, the most obvious change was an enhanced clarity of the cell membranes.


2013 ◽  
Vol 13 (4) ◽  
pp. 369-384

Many Public Aquaria have been designed and constructed all over the world during the last three decades. The serial arrangement of relatively small, rectangular, concrete tanks has been replaced by fewer large, irregularly shaped tanks, replicating habitats. The “taxonomic concept” of displaying specimens in the old aquaria has now been succeeded by the more ecological, “community concept” type of display. At the same time most of the “old aquaria” have been renovated. Aquarium missions have also been broadened nowadays including research, conservation and education. Aquaria are ideal places for research on husbandry, life cycles, reproduction, behavior, autoecology and fish pathology. Collaboration with Universities and Research Centers increases the research potential in scientific disciplines such as ecology, genetics, physiology and biochemistry. Collaboration also provides mutual benefits in both infrastructure and personnel: The research background in aquaria also forms a sound platform to materialize conservation projects, focusing either on the ex-situ conservation of animals in the aquaria or on environmental protection of surrounding areas and reintroduction of endangered species. In addition to formal educational opportunities, non formal education to visitors, schools and undergraduates seems to become a major mission of aquaria. Aquarium tank displays, preserved biological material, film projections, seminars / lectures and book magazine publications enhance environmental awareness, encouraging people to adopt Environmentally Responsible Behavior. All these missions are feasible because most public aquaria are making a good profit mainly due to their high popularity. There are also benefits for the community in the area; aquaria have enlivened declining water front areas and increased the income of tourist resorts mainly by “stretching out” the tourist season. In the present work the objectives of a public aquarium are reviewed and the main infrastructure subsystems and operational procedures are described; Know how on aquarium systems can also be applied in research laboratories of academic institutions if live organisms have to be kept for experimentation. Aquarium missions on research, conservation and education are discussed.


2020 ◽  
Vol 27 (20) ◽  
pp. 3330-3345
Author(s):  
Ana G. Rodríguez-Hernández ◽  
Rafael Vazquez-Duhalt ◽  
Alejandro Huerta-Saquero

Nanomaterials have become part of our daily lives, particularly nanoparticles contained in food, water, cosmetics, additives and textiles. Nanoparticles interact with organisms at the cellular level. The cell membrane is the first protective barrier against the potential toxic effect of nanoparticles. This first contact, including the interaction between the cell membranes -and associated proteins- and the nanoparticles is critically reviewed here. Nanoparticles, depending on their toxicity, can cause cellular physiology alterations, such as a disruption in cell signaling or changes in gene expression and they can trigger immune responses and even apoptosis. Additionally, the fundamental thermodynamics behind the nanoparticle-membrane and nanoparticle-proteins-membrane interactions are discussed. The analysis is intended to increase our insight into the mechanisms involved in these interactions. Finally, consequences are reviewed and discussed.


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