scholarly journals Stable isotope phosphate labelling of diverse metabolites is enabled by a family of 18O-phosphoramidites

2021 ◽  
Author(s):  
Thomas Haas ◽  
Stephan Mundinger ◽  
Danye Qiu ◽  
Nikolaus Jork ◽  
Kevin Ritter ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Stable Isotope ◽  
State Of The Art ◽  
Inositol Phosphates ◽  
Neutral Loss ◽  
Ion Source ◽  
Quadrupole Mass ◽  
Quantitative Mass Spectrometry ◽  
Stable Isotope Labelling ◽  
Phosphorylated Compounds

Stable isotope labelling is state-of-the-art in quantitative mass spectrometry, yet often accessing the required standards is cumbersome and very expensive. As 18O can be derived from heavy water (H218O), it is comparably cheap and particularly suited for labelling of phosphorylated compounds, provided the introduction is straight-forward and phosphate neutral loss in the ion source can be avoided. Here, a unifying synthetic concept for 18O-labelled phosphates is presented, based on a family of modified 18O2‑phosphoramidite reagents. This flexible toolbox offers access to major classes of biologically highly relevant phosphorylated metabolites as their isotopologues including - but not limited to - nucleotides, inositol phosphates, -pyrophosphates, and inorganic polyphosphates. 18O-enrichment ratios >95% and good yields are obtained consistently in gram-scale reactions, while enabling late-stage labelling. We demonstrate the utility of the 18O labelled inositol phosphates and pyrophosphates by assignment of these metabolites from different biological matrices, such as mammalian cell lysates, slime mold and plant samples. We demonstrate that phosphate neutral loss is negligible in an analytical setup employing capillary electrophoresis electrospray ionization triple quadrupole mass spectrometry.

scholarly journals Probing rapid carbon fixation in fast-growing seaweed Ulva meridionalis using stable isotope 13C-labelling

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Shuntaro Tsubaki ◽  
Hiroshi Nishimura ◽  
Tomoya Imai ◽  
Ayumu Onda ◽  
Masanori Hiraoka
Keyword(s):  
Mass Spectrometry ◽  
Stable Isotope ◽  
Carbon Fixation ◽  
High Growth ◽  
Gas Chromatography Mass Spectrometry ◽  
Ion Cyclotron Resonance ◽  
Stable Isotope Labelling ◽  
Ft Icr Ms ◽  
Ft Icr ◽  
C Nmr

AbstractThe high growth rate of Ulva seaweeds makes it a potential algal biomass resource. In particular, Ulva meridionalis grows up to fourfold a day. Here, we demonstrated strong carbon fixation by U. meridionalis using 13C stable isotope labelling and traced the 13C flux through sugar metabolites with isotope-ratio mass spectrometry (IR-MS), Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS), 13C-nuclear magnetic resonance spectrometry (13C-NMR), and gas chromatography-mass spectrometry (GC–MS). U. meridionalis was first cultured in 13C-labelled enriched artificial seawater for 0–12 h, and the algae were collected every 4 h. U. meridionalis grew 1.8-fold (dry weight), and the 13C ratio reached 40% in 12 h, whereas 13C incorporation hardly occurred under darkness. At the beginning of the light period, 13C was incorporated into nucleic diphosphate (NDP) sugars in 4 h, and 13C labelled peaks were identified using FT-ICR-MS spectra. Using semiquantitative 13C-NMR measurements and GC–MS, 13C was detected in starch and matrix polysaccharides after the formation of NDP sugars. Moreover, the 14:10 light:dark regime resulted into 85% of 13C labelling was achieved after 72 h of cultivation. The rapid 13C uptake by U. meridionalis shows its strong carbon fixation capacity as a promising seaweed biomass feedstock.

scholarly journals Analytical Considerations of Stable Isotope Labelling in Lipidomics

Biomolecules ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 151 ◽  
Author(s):  
Alexander Triebl ◽  
Markus Wenk
Keyword(s):  
Mass Spectrometry ◽  
Stable Isotope ◽  
Clinical Settings ◽  
Stable Isotope Labelling ◽  
Stable Isotope Label ◽  
Isotope Labelling ◽  
Isotope Label ◽  
Lipid Biomarker ◽  
Staple Technique ◽  
Biomarker Research

Over the last two decades, lipids have come to be understood as far more than merely components of cellular membranes and forms of energy storage, and are now also being implicated to play important roles in a variety of diseases, with lipid biomarker research one of the most widespread applications of lipidomic techniques both in research and in clinical settings. Stable isotope labelling has become a staple technique in the analysis of small molecule metabolism and dynamics, as it is the only experimental setup by which biosynthesis, remodelling and degradation of biomolecules can be directly measured. Using state-of-the-art analytical technologies such as chromatography-coupled high resolution tandem mass spectrometry, the stable isotope label can be precisely localized and quantified within the biomolecules. The application of stable isotope labelling to lipidomics is however complicated by the diversity of lipids and the complexity of the necessary data analysis. This article discusses key experimental aspects of stable isotope labelling in the field of mass spectrometry-based lipidomics, summarizes current applications and provides an outlook on future developments and potential.

P3-071: IN VIVO STABLE ISOTOPE LABELING AND QUANTITATIVE MASS SPECTROMETRY IMAGING OF Aβ PLAQUE DEPOSITION AND TAUOPATHY IN HUMAN AD BRAIN

2006 ◽  
Vol 14 (7S_Part_20) ◽  
pp. P1091-P1092
Author(s):  
Norelle C. Wildburger ◽  
Greg S. Day ◽  
Wendy Sigurdson ◽  
Melissa Sullivan ◽  
Amanda Peters ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Stable Isotope ◽  
Mass Spectrometry Imaging ◽  
Isotope Labeling ◽  
Stable Isotope Labeling ◽  
Quantitative Mass Spectrometry ◽  
Plaque Deposition

Rapid and sensitive monitoring of biocatalytic reactions using ion mobility mass spectrometry

The Analyst ◽  
2016 ◽  
Vol 141 (8) ◽  
pp. 2351-2355 ◽  
Author(s):  
Cunyu Yan ◽  
Jason W. Schmidberger ◽  
Fabio Parmeggiani ◽  
Shaneela A. Hussain ◽  
Nicholas J. Turner ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Stable Isotope ◽  
High Throughput ◽  
Ion Mobility ◽  
Ion Mobility Mass Spectrometry ◽  
Direct Infusion ◽  
Stable Isotope Labelling ◽  
Isotope Labelling

The combination of stable isotope labelling with direct infusion ion mobility mass spectrometry enabled high-throughput and sensitive monitoring of biocatalytic reactions.

scholarly journals Analysis of alkyl esters of p-hydroxybenzoic acid (parabens) in baby teethers via gas chromatography-quadrupole mass spectrometry (GC-qMS) using a stable isotope dilution assay (SIDA)

2016 ◽  
Vol 8 (17) ◽  
pp. 3466-3474 ◽  
Author(s):  
Theodoros Potouridis ◽  
Elisabeth Berger ◽  
Wilhelm Püttmann
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Stable Isotope ◽  
Hydroxybenzoic Acid ◽  
Methyl Ethyl ◽  
Quadrupole Mass ◽  
Alkyl Esters ◽  
Stable Isotope Dilution ◽  
Isotope Dilution Assay ◽  
Dilution Assay

The present work describes an analytical method for the analysis of methyl-, ethyl- andn-propylparaben in plastic and gel material from baby teethers filled with cooling gel.

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