scholarly journals The Effect of Lighted Incubation on Ribonucleic Acid, Deoxyribonucleic Acid, and Protein Content of Chick Embryos

1981 ◽  
Vol 60 (5) ◽  
pp. 1089-1091 ◽  
Author(s):  
MARILYN A. COLEMAN ◽  
ROBERT C. SMITH ◽  
GAYNER R. McDANIEL
Keyword(s):  
Protein Content ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Chick Embryos

SPECIES DIFFERENCES IN THE DEOXYRIBONUCLEIC AND RIBONUCLEIC ACID CONTENTS OF LIVERS OF NON-PREGNANT AND PREGNANT MICE, GUINEA-PIGS AND CATS

1953 ◽  
Vol 9 (1) ◽  
pp. 45-51 ◽  
Author(s):  
ROSA M. CAMPBELL ◽  
H. W. KOSTERLITZ
Keyword(s):  
Guinea Pig ◽  
Protein Content ◽  
Liver Cell ◽  
Ribonucleic Acid ◽  
Guinea Pigs ◽  
Deoxyribonucleic Acid ◽  
Species Differences ◽  
Liver Cells ◽  
Maternal Liver ◽  
Pregnant Mice

1. The protein content of liver cells is almost independent of the size of the animal (mice, cats and previous results on rats, Campbell & Kosterlitz [1949]), and varies with the amount of protein eaten. 2. As has already been shown for rats, the ribonucleic acid ('RNA') content of the liver cells of non-pregnant mice, guinea-pigs and cats varies directly with the protein content of the cells. For a given protein content the mouse and rat have more RNA than the guinea-pig and cat. 3. During pregnancy there is a rise of the deoxyribonucleic acid ('DNA') content of the livers and in the protein content of the liver cells of mice (and rats), but not of guinea-pigs. 4. An excess of RNA over that predicted from the protein content of the liver cell has previously been found for the rat during pregnancy, and ascribed to the action of a placental factor on the maternal liver. A similar excess of RNA has now been observed in the mouse and, to a less extent, in the guinea-pig. It appears to be absent in the cat. 5. Possible causes of some of these species differences are considered.

Nucleic Acids in Grass and Grass Silage

1988 ◽  
Vol 1988 ◽  
pp. 86-86
Author(s):  
A B McAllan ◽  
G D Braithwaite
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Protein Content ◽  
Ribonucleic Acid ◽  
Protein Fraction ◽  
Dry Matter ◽  
Deoxyribonucleic Acid ◽  
Total N ◽  
Grass Silage ◽  
Microbial N

Little attention has been directed at defining the ‘protein’ fraction of silages. This component is normally estimated by fractionation based on solubility characteristics and under the conditions most commonly used, nucleic acids (ribonucleic acid (RNA) and deoxyribonucleic acid (DNA)) would appear in the protein fraction. Grasses and legumes can contain appreciable amounts of nucleic acids ranging from 11-29 and 19-53 g/kg dry matter respectively (McAllan, 1982). No information is available as to the effects of ensilage on these nucleic acids. Microbes also contain appreciable amounts of nucleic acids which can account for 150-200 gN/kg total-N of the cell and these amounts may vary according to the stage of growth. It has been suggested (Ullrich, 1982) that microbial-N contribution to the total-N content of silage is as much as 220-280 g/kg. Thus the total amount of silage ‘protein-N’ present in the form of nucleic acid-N (from both plant and microbes) could be appreciable leading to a considerable overestimation of the ‘protein’ content of the silage.

Composition of Methanospirillum hungatii GP1 during growth on different media

1980 ◽  
Vol 26 (5) ◽  
pp. 577-582 ◽  
Author(s):  
C. Breuil ◽  
G. B. Patel
Keyword(s):  
Protein Content ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Synthetic Medium ◽  
Density Measurement ◽  
Dry Weight ◽  
Weight Basis ◽  
Total Carbohydrate ◽  
Mineral Salts

Growth of Methanospirillum hungatii GP1 as determined by optical density measurement was comparable to growth assessed by cell dry weight, ribonucleic acid content, and deoxyribonucleic acid content. Cultivation of M. hungatii on synthetic medium containing mineral salts, vitamins, and acetic acid indicated that, on a dry weight basis, cell constituents such as protein (71%), ribonucleic acid (15.8%), deoxyribonucleic acid (1.6%), and total carbohydrate (3.2%) did not vary significantly with the growth phase. Cells grown in the synthetic medium supplemented with yeast extract and tryptone had slightly higher protein content (76%), but the concentrations of the other cell constituents were similar and did not fluctuate much during growth. Nitrogen limiting growth resulted in somewhat lower ribonucleic acid content as well as slightly higher protein content than that in cells grown in nonlimiting medium. Methanospirillum hungatii did not accumulate any of the commonly known reserve materials under nitrogen or carbon and hydrogen limiting growth.

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