Early post-hatch survival and humoral immune response of layer chickens when in ovo vaccinated with strain F Mycoplasma gallisepticum

The effect of the in ovo application of selected prebiotics and synbiotics on the humoral immune response against T-dependent (SRBC) and T-independent (dextran) antigens and delayed-type hypersensitivity (DTH) to phytohemagglutinin was studied. On the 12th day of incubation, 800 eggs (Ross 308) were divided into five groups and injected into the egg air chamber with prebiotic inulin (Pre1), Bi2tos (Pre2), a synbiotic composed of inulin and Lactococcus lactis subsp. lactis IBB SL1 (Syn1), a synbiotic composed of Bi2tos and L. lactis subsp. cremoris IBB SC1 (Syn2), and physiological saline (control group; C). The chickens were immunized twice at the 7th and 21st day of life with SRBC and dextran. A DTH test was performed on the 7th, 21st, and 35th day. The application of prebiotics and synbiotics had no significant effect on the humoral immune response. SRBC-immunized in ovo Pre1- and Pre2-treated chickens showed significantly higher serum IgG levels than the control. A significant effect on the DTH reaction was detected on the 7th (Pre1 < C) and 21st (Pre2 > Syn2) day. However; Bi2tos may transiently stimulate the cellular immune response on the 21st day. It may be concluded that the application of inulin in an egg air chamber on the 12th day of incubation may stimulate the secondary immune response. The inulin-treated group exhibited a lower mortality rate than the control group.

Download Full-text

Humoral Immune Response of Turkeys to Strain S6 and a Variant Mycoplasma gallisepticum Studied by Immunoblotting

Avian Diseases ◽

10.2307/1591718 ◽

1992 ◽

Vol 36 (1) ◽

pp. 69 ◽

Cited By ~ 5

Author(s):

Alan P. Avakian ◽

David H. Ley ◽

Mary Ann T. McBride

Keyword(s):

Immune Response ◽

Humoral Immune Response ◽

Mycoplasma Gallisepticum ◽

Humoral Immune

Download Full-text

Oral immunoadjuvant activity ofLactobacillus caseisubsp.caseiin dextran-fed layer chickens

British Journal Of Nutrition ◽

10.1079/bjn20051629 ◽

2006 ◽

Vol 95 (2) ◽

pp. 430-434 ◽

Cited By ~ 16

Author(s):

Tomohiko Ogawa ◽

Yasuyuki Asai ◽

Hiromi Sakamoto ◽

Kenji Yasuda

Keyword(s):

Immune Response ◽

Humoral Immune Response ◽

Specific Substrate ◽

Infectious Bronchitis ◽

Humoral Immune ◽

Humoral Immune Responses ◽

Actual Application ◽

Colony Forming Units ◽

Inactivated Vaccines ◽

Layer Chickens

We recently reported that synbioticLactobacillus caseisubsp.caseitogether with specific substrate dextran elicited an enhancement in humoral immune response against bovine serum albumin (BSA) as a model antigen in BALB/c mice. The present study was designed to evaluate the oral immunoadjuvant effects of the synbiotic in layer chickens. Using a PCR assay,L. caseisubsp.caseiwas detected specifically in the intestinal chyme of chickens (10d of age, Julia strain) fedad libitumon a diet supplemented with 75mg dextran/kg (dextran-supplemented diet, DSD) and administered orally with 107colony-forming units (CFU)L. caseisubsp.caseiin 0·1ml PBS with the aid of an intubation needle at 1, 2 and 3d of age. Furthermore, oral administration of 107CFUL. caseisubsp.caseiat 1–3d of age significantly enhanced the production of anti-BSA antibody in DSD-fed chickens (60d of age) administered orally with 1mg BSA at 32 and 33d of age and subcutaneously with 5μg BSA at 33d of age. In addition, among bacterial numbers tested, 106CFUL. caseisubsp.caseitogether with dextran induced an effective increase in humoral immune response to mixed inactivated vaccines against Newcastle disease and avian infectious bronchitis, and the treatment may be advantageous in protecting against these infectious diseases in chickens in actual application. These results suggest that dietary supplementation ofL. caseisubsp.caseiwith dextran leads to immunomodulation of humoral immune responses.

Download Full-text