Growth and lipid content of two freshwater microalgae: Asterococcus korschikoffii Ettl and Vischeria helvetica (Vischer & Pascher) Hibberd

Short-term deleterious effects of standard isolation and cultivation methods on new tropical freshwater microalgae strains

10.7287/peerj.preprints.2881v2 ◽

2017 ◽

Author(s):

M. Magdalena Aray-Andrade ◽

Miguel I Uyaguari-Diaz ◽

J. Rafael Bermúdez Monsalve

Keyword(s):

Growth Rate ◽

Lipid Content ◽

Genetic Characterization ◽

Potential Effect ◽

Tropical Species ◽

Freshwater Microalgae ◽

Lower Growth ◽

Tropical Regions ◽

Significant Difference ◽

Tropical Freshwater

Algae with potential biotechnological applications in different industries are commonly isolate from the environment in order to obtain pure (axenic) stocks that can be safely stored for long periods of time. To obtain axenic cultures, antibiotics are frequently used, and cryopreservation is applied to keep standing stocks. However, many of these now standard methods were developed using strains coming from pristine-to-low intervened environments and cold-to-temperate regions. Thus, it is still not well understand the potential effect of said methods on the life cycle and biochemical profile of algae isolates from hiper-eutrophic and constant high-temperature tropical regions, which could potentially render them unsuitable for their intended biotechnological application. In this study, we conducted a genetic characterization (18S rRNA) and evaluated the effect of purification (use of the antibiotic chloramphenicol, CPA) and cryopreservation (dimethyl sulfoxide (DMSO)-sucrose mix and Glycerol) on the growth rate and lipid content of four new tropical freshwater algal isolates: Chorella sp. M2, Chlorella sp. M6, Scenedesmus sp. R3, and Coelastrella sp. A2, from the Ecuadorian coast. The genetic characterization showed a clear discrimination between strains. All strains cultured with CPA had a lower growth rate. After cryopreservation Coelastrella sp. A2 did not grow with both methods; Chorella sp. M2, Chlorella sp. M6 and Scenedesmus sp. R3 presented no significant difference in growth rate between the cryopreservants. A significantly higher lipid content was observed in biomass cryopreserved with glycerol in relation to DMSO-sucrose, with Chorella sp. M2 and Chlorella sp. M6 having twice as much in the first treatment. The results highlight the relevance of choosing an appropriate method for storage as the materials used can affect the biological performance of different tropical species, although is still to determine if the effects observed here are long lasting in subsequent cultures of these algae.

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Long-Term Toxicity of ZnO Nanoparticles on Scenedesmus rubescens Cultivated in Semi-Batch Mode

Nanomaterials ◽

10.3390/nano10112262 ◽

2020 ◽

Vol 10 (11) ◽

pp. 2262

Author(s):

Andriana F. Aravantinou ◽

Fytoula Andreou ◽

Ioannis D. Manariotis

Keyword(s):

Lipid Content ◽

Zno Nanoparticles ◽

Nutrient Removal ◽

Zno Nps ◽

Freshwater Microalgae ◽

Batch Mode ◽

Algal Lipid ◽

Scenedesmus Rubescens ◽

Microalgae Growth

The scope of this study was to investigate the toxic effects of zinc oxide (ZnO) nanoparticles (NPs) on freshwater microalgae, in long-term semi-batch feeding mode at two different hydraulic retention times (HRTs) (20 and 40 days). A freshwater microalgae, Scenedesmus rubescens, was employed and exposed to a semi-continuous supply of ZnO NPs at a low concentration of 0.081 mg/L for a period of 28 d. Experiments were conducted under controlled environmental conditions. Τhe impact of ZnO NPs on S. rubescens, which was assessed in terms of nutrient removal, biomass growth, and algal lipid content. Semi-batch mode cultures showed that low ZnO NP concentrations at an HRT of 40 d did not have any negative effect on microalgae growth after the fourth day of culture. In contrast, algal growth was inhibited up to 17.5% at an HRT of 20 d in the presence of ZnO NPs. This might be attributed to the higher flow rate applied and ZnO NPs load. A positive correlation between nutrient removal and microalgae growth was observed. The algal lipid content was, in most cases, higher in the presence of ZnO NPs at both HRTs, indicating that even low ZnO NPs concentration cause stress resulting in higher lipid content.

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Ruminal in vitro gas production, dry matter digestibility, methane abatement potential, and fatty acid biohydrogenation of six species of microalgae

Canadian Journal of Animal Science ◽

10.1139/cjas-2015-0141 ◽

2016 ◽

Vol 96 (3) ◽

pp. 354-363 ◽

Cited By ~ 10

Author(s):

U.Y. Anele ◽

W.Z. Yang ◽

P.J. McGinn ◽

S.M. Tibbetts ◽

T.A. McAllister

Keyword(s):

Fatty Acid ◽

Linolenic Acid ◽

Lipid Content ◽

Dry Matter ◽

Gas Production ◽

Total Carbohydrate ◽

Freshwater Microalgae ◽

Dry Matter Digestibility ◽

In Vitro Gas Production

This study evaluated the composition, digestibility [dry matter digestibility (DMD)], CH4 abatement potential, and fatty acid biohydrogenation of six species of microalgae. Lipid content ranged from 115 g kg−1 dry matter (DM) (Scenedesmus sp. AMDD) to 361 g kg−1 DM (Tetracystis sp.), while Scenedesmus sp. AMDD had the highest carbohydrate (364 g kg−1 DM) and fibre content (277 g kg−1 DM). Gas production was highest (P < 0.001) for Micractinium reisseri and Chlorella vulgaris. In vitro DMD ranged from 654 g kg−1 for Scenedesmus sp. AMDD to 797 g kg−1 for Nannochloris bacillaris. Total CH4 differed (P < 0.001) among microalgae, ranging from 1.76 mL g−1 DM for Tetracystis sp. to 4.07 mL g−1 DM for M. reisseri. Nannochloropsis granulata (marine) had higher myristic, palmitoleic, and eicosapentaenoic acid levels than freshwater microalgae. Levels of α-linolenic acid were higher in Scenedesmus sp. AMDD than all other microalgae. CH4 production negatively correlated (P < 0.05) with levels of total carbohydrate, oleic, and α-linolenic acid. Despite having a lower lipid content, CH4 reductions with Scenedesmus sp. AMDD were comparable to Tetracystis sp. and N. bacillaris. Reductions in CH4 with Tetracystis sp. and N. bacillaris occurred without a decline in DMD, suggesting that overall microbial activity was not inhibited.

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Short-term deleterious effects of standard isolation and cultivation methods on new tropical freshwater microalgae strains

PeerJ ◽

10.7717/peerj.5143 ◽

2018 ◽

Vol 6 ◽

pp. e5143

Author(s):

M. Magdalena Aray-Andrade ◽

Miguel I. Uyaguari-Diaz ◽

J. Rafael Bermúdez

Keyword(s):

Growth Rate ◽

Lipid Content ◽

Potential Effect ◽

Morphological Characterization ◽

Tropical Species ◽

Freshwater Microalgae ◽

Lower Growth ◽

Tropical Regions ◽

Significant Difference ◽

Tropical Freshwater

Algae with potential biotechnological applications in different industries are commonly isolated from the environment in order to obtain pure (axenic) stocks that can be safely stored for long periods of time. To obtain axenic cultures, antibiotics are frequently employed, and cryopreservation is applied to preserve standing stocks. However, many of these now standard methods were developed using strains derived from pristine to near-pristine environments and cold to temperate regions. The potential effect of the said methods on the life cycle and biochemical profile of algae isolates from hyper-eutrophic and constant high-temperature tropical regions is not well understood. These effects could potentially render them unsuitable for their intended biotechnological application. In this study, we conducted a genetic characterization (18S rRNA) and evaluated the effect of purification (the use of the antibiotic chloramphenicol, CAP) and cryopreservation (dimethyl sulfoxide; DMSO–sucrose mix and glycerol) on the growth rate and lipid content of three new tropical freshwater algal isolates: Chorella sp. M2, Chlorella sp. M6, and Scenedesmus sp. R3, obtained from the Ecuadorian coast. The genetic and morphological characterization revealed a clear discrimination between these strains. All strains cultured with CAP exhibited a lower growth rate. Subsequent to cryopreservation, Chorella sp. M2, Chlorella sp. M6, and Scenedesmus sp. R3 presented no significant difference in growth rate between the cryopreservants. Further, a significantly higher lipid content was observed in the biomass cryopreserved with glycerol in relation to the DMSO–sucrose, with Chorella sp. M2 and Chlorella sp. M6 having twice as much as they had in the first treatment. These results highlight the relevance of selecting an appropriate method for storage, as the materials used can affect the biological performance of different tropical species, although it is still to be determined if the effects observed in this study are long lasting in subsequent cultures of these algae.

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Short-term deleterious effects of standard isolation and cultivation methods on new tropical freshwater microalgae strains

10.7287/peerj.preprints.2881v1 ◽

2017 ◽

Author(s):

M. Magdalena Aray-Andrade ◽

Miguel I Uyaguari-Diaz ◽

J. Rafael Bermúdez Monsalve

Keyword(s):

Growth Rate ◽

Lipid Content ◽

Potential Effect ◽

Tropical Species ◽

Freshwater Microalgae ◽

Lower Growth ◽

Tropical Regions ◽

Chlorella Sp ◽

Biological Performance ◽

Tropical Freshwater

Algae with potential biotechnological applications in different industries are commonly isolate 5 from the environment in order to obtain pure (axenic) stocks that can be safely stored for long 6 periods of time. To obtain axenic cultures, antibiotics are frequently used, and cryopreservation 7 is applied to keep standing stocks. However, many of these now standard methods were 8 developed using strains coming from pristine-to-low intervened environments and cold-to- 9 temperate regions. Thus, it is still not well understand the potential effect of said methods on the 10 life cycle and biochemical profile of algae isolates from hiper-eutrophic and constant high- 11 temperature tropical regions, which could potentially render them unsuitable for their intended 12 biotechnological application. In this study, we did a genetic characterization (18s mDNA) and 13 evaluated the effect of purification (use of the antibiotic chloramphenicol, CPA) and 14 cryopreservation (dimethyl sulfoxide (DMSO)-sucrose mix and Glycerol) on the growth rate and 15 lipid content of four new tropical freshwater algal isolates: Chorella sp. M2, Chlorella sp. M6, 16 Scenedesmus sp. R3, and Coelastrella sp. A2, from the Ecuadorian coast. The genetic 17 characterization showed a clear discrimination between strains. All strains cultured with CPA 18 had a lower growth rate. After cryopreservation Coelastrella sp. A2 did not grow with both 19 methods; Chorella sp. M2, Chlorella sp. M6 and Scenedesmus sp. R3 presented no significant 20 difference in growth rate between the cryopreservants. A significantly higher lipid content was 21 observed in biomass cryopreserved with glycerol in relation to DMSO-sucrose, with Chorella sp. 22 M2 and Chlorella sp. M6 having twice as much in the first treatment. The results highlight the 23 relevance of choosing an appropriate method for storage as the materials used can affect the 3 1 biological performance of different tropical species, although is still to determine if the effects 2 observed here are long lasting in subsequent cultures of these algae.

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A synergic approach for nutrient recovery and biodiesel production by the cultivation of microalga species in the fertilizer plant wastewater

Scientific Reports ◽

10.1038/s41598-019-55748-w ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 1

Author(s):

Indu Ambat ◽

Sabina Bec ◽

Elina Peltomaa ◽

Varsha Srivastava ◽

Anne Ojala ◽

...

Keyword(s):

Lipid Content ◽

Algal Species ◽

Acid Methyl Ester ◽

Biomass Concentration ◽

Biomass Productivity ◽

Biodiesel Production ◽

Freshwater Microalgae ◽

Fertilizer Plant ◽

Solvent Extraction Method

AbstractThe combination of wastewater treatment and biodiesel production using algal cultivation was studied in the present work. The two main goals of the work were achieved by the cultivation of freshwater microalgae such as Chlamydomonas sp., Scenedesmus ecornis, and Scenedesmus communis in two different dilutions of fertilizer plant wastewater (FWWD1 and FWWD2) collected from Yara Suomi Oy, Finland. The growth pattern of different algal species in FWWD1 and FWWD2 was observed. The effect of pH on biomass concentration, lipid content, biomass productivity, and lipid productivity by all three algal species in FWWD1 and FWWD2 were monitored. The maximum biomass concentration and productivity were observed in FWWD1 at pH7.5 for Chlamydomonas sp. and at pH 8.5 for S. ecornis and S. communis. The maximum lipid content was detected in Chlamydomonas sp at pH5.5, followed by S. ecornis and then S. communis at pH 7.5 in FWWD2 obtained after co-solvent extraction method. The most significant removal percentage of COD by all algal species were observed in FWWD1, whereas the highest removal percentage of TN and TP were detected in FWWD2, respectively. The fatty acid methyl ester (FAME) characterization of each algal species in FWWD1 and FWWD2 at their optimum pH was investigated to determine the quality of obtained biodiesel.

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Increased pigment and lipid content, lipid variety, and cell and population size of the microalgae Chlorella spp. when co-immobilized in alginate beads with the microalgae-growth-promoting bacterium Azospirillum brasilense

Canadian Journal of Microbiology ◽

10.1139/w02-051 ◽

2002 ◽

Vol 48 (6) ◽

pp. 514-521 ◽

Cited By ~ 144

Author(s):

Luz E de-Bashan ◽

Yoav Bashan ◽

Manuel Moreno ◽

Vladimir K Lebsky ◽

Jose J Bustillos

Keyword(s):

Fatty Acids ◽

Population Size ◽

Cell Size ◽

Lipid Content ◽

Azospirillum Brasilense ◽

Alginate Beads ◽

Freshwater Microalgae ◽

Bacterial Immobilization ◽

Growth Promoting ◽

Microalgae Growth

Three strains of the freshwater microalgae used for wastewater treatment, Chlorella vulgaris and Chlorella sorokiniana co-immobilized separately in alginate beads with the microalgae-growth-promoting bacterium Azospirillum brasilense Cd, resulted in significant changes in microalgal-population size, cell size, cell cytology, pigment, lipid content, and the variety of fatty acids produced in comparison with microalgae immobilized in alginate without the bacterium. Cells of C. vulgaris UTEX 2714 did not change in size, but the population size within the beads significantly increased. On the other hand, C. vulgaris UTEX 395 cells grew 62% larger, but their numbers did not increase. The population of C. sorokiniana UTEX 1602 increased, but not their cell size. The content of pigments chlorophyll a andb, lutein, and violoaxanthin increased in all microalgal species. The lipid content also significantly increased in all three strains, and the number of different fatty acids in the microalgae increased from four to eight. This study indicates that the microalgae-growth-promoting bacterium induced significant changes in the metabolism of the microalgae.Key words: alginate, Azospirillum, Chlorella, bacterial immobilization, microalgae, wastewater.

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Short-term deleterious effects of standard isolation and cultivation methods on new tropical freshwater microalgae strains

10.7287/peerj.preprints.2881 ◽

2017 ◽

Author(s):

M. Magdalena Aray-Andrade ◽

Miguel I Uyaguari-Diaz ◽

J. Rafael Bermúdez Monsalve

Keyword(s):

Growth Rate ◽

Lipid Content ◽

Genetic Characterization ◽

Potential Effect ◽

Tropical Species ◽

Freshwater Microalgae ◽

Lower Growth ◽

Tropical Regions ◽

Significant Difference ◽

Tropical Freshwater

Algae with potential biotechnological applications in different industries are commonly isolate from the environment in order to obtain pure (axenic) stocks that can be safely stored for long periods of time. To obtain axenic cultures, antibiotics are frequently used, and cryopreservation is applied to keep standing stocks. However, many of these now standard methods were developed using strains coming from pristine-to-low intervened environments and cold-to-temperate regions. Thus, it is still not well understand the potential effect of said methods on the life cycle and biochemical profile of algae isolates from hiper-eutrophic and constant high-temperature tropical regions, which could potentially render them unsuitable for their intended biotechnological application. In this study, we conducted a genetic characterization (18S rRNA) and evaluated the effect of purification (use of the antibiotic chloramphenicol, CPA) and cryopreservation (dimethyl sulfoxide (DMSO)-sucrose mix and Glycerol) on the growth rate and lipid content of four new tropical freshwater algal isolates: Chorella sp. M2, Chlorella sp. M6, Scenedesmus sp. R3, and Coelastrella sp. A2, from the Ecuadorian coast. The genetic characterization showed a clear discrimination between strains. All strains cultured with CPA had a lower growth rate. After cryopreservation Coelastrella sp. A2 did not grow with both methods; Chorella sp. M2, Chlorella sp. M6 and Scenedesmus sp. R3 presented no significant difference in growth rate between the cryopreservants. A significantly higher lipid content was observed in biomass cryopreserved with glycerol in relation to DMSO-sucrose, with Chorella sp. M2 and Chlorella sp. M6 having twice as much in the first treatment. The results highlight the relevance of choosing an appropriate method for storage as the materials used can affect the biological performance of different tropical species, although is still to determine if the effects observed here are long lasting in subsequent cultures of these algae.

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