A collaborative neuroinformatics platform for distribution and characterization of transgenic mouse lines for cell type specific neuroscience research

AbstractThe basolateral amygdalar complex (BLA) is implicated in behaviors ranging from fear acquisition to addiction. Optogenetic methods have enabled the association of circuit-specific functions to uniquely connected BLA cell types. Thus, a systematic and detailed connectivity profile of BLA projection neurons to inform granular, cell type-specific interrogations is warranted. Here, we apply machine-learning based computational and informatics analysis techniques to the results of circuit-tracing experiments to create a foundational, comprehensive BLA connectivity map. The analyses identify three distinct domains within the anterior BLA (BLAa) that house target-specific projection neurons with distinguishable morphological features. We identify brain-wide targets of projection neurons in the three BLAa domains, as well as in the posterior BLA, ventral BLA, posterior basomedial, and lateral amygdalar nuclei. Inputs to each nucleus also are identified via retrograde tracing. The data suggests that connectionally unique, domain-specific BLAa neurons are associated with distinct behavior networks.

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Cytometric Profiling of CD133+ Cells in Human Colon Carcinoma Cell Lines Identifies a Common core Phenotype and Cell Type-specific Mosaics

The International Journal of Biological Markers ◽

10.5301/jbm.5000020 ◽

2013 ◽

Vol 28 (3) ◽

pp. 267-273 ◽

Cited By ~ 11

Author(s):

Marica Gemei ◽

Rosa Di Noto ◽

Peppino Mirabelli ◽

Luigi Del Vecchio

Keyword(s):

Colorectal Cancer ◽

Stem Cells ◽

Cancer Stem Cells ◽

Colon Carcinoma ◽

Cell Lines ◽

Carcinoma Cell ◽

Cell Type ◽

Carcinoma Cell Lines ◽

Cell Type Specific

In colorectal cancer, CD133+ cells from fresh biopsies proved to be more tumorigenic than their CD133– counterparts. Nevertheless, the function of CD133 protein in tumorigenic cells seems only marginal. Moreover, CD133 expression alone is insufficient to isolate true cancer stem cells, since only 1 out of 262 CD133+ cells actually displays stem-cell capacity. Thus, new markers for colorectal cancer stem cells are needed. Here, we show the extensive characterization of CD133+ cells in 5 different colon carcinoma continuous cell lines (HT29, HCT116, Caco2, GEO and LS174T), each representing a different maturation level of colorectal cancer cells. Markers associated with stemness, tumorigenesis and metastatic potential were selected. We identified 6 molecules consistently present on CD133+ cells: CD9, CD29, CD49b, CD59, CD151, and CD326. By contrast, CD24, CD26, CD54, CD66c, CD81, CD90, CD99, CD112, CD164, CD166, and CD200 showed a discontinuous behavior, which led us to identify cell type-specific surface antigen mosaics. Finally, some antigens, e.g. CD227, indicated the possibility of classifying the CD133+ cells into 2 subsets likely exhibiting specific features. This study reports, for the first time, an extended characterization of the CD133+ cells in colon carcinoma cell lines and provides a “dictionary” of antigens to be used in colorectal cancer research.

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Characterization of cell type-specific S100B expression in the mouse olfactory bulb

Cell Calcium ◽

10.1016/j.ceca.2020.102334 ◽

2021 ◽

Vol 94 ◽

pp. 102334

Author(s):

Xin Su ◽

Tamara Vasilkovska ◽

Nicole Fröhlich ◽

Olga Garaschuk

Keyword(s):

Olfactory Bulb ◽

Cell Type ◽

Cell Type Specific

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Implantable Optrode Array for Optogenetic Modulation and Electrical Neural Recording

Micromachines ◽

10.3390/mi12060725 ◽

2021 ◽

Vol 12 (6) ◽

pp. 725

Author(s):

Saeyeong Jeon ◽

Youjin Lee ◽

Daeho Ryu ◽

Yoon Kyung Cho ◽

Yena Lee ◽

...

Keyword(s):

Light Emitting Diode ◽

Neural Recording ◽

Electrophysiological Recording ◽

Light Emitting ◽

Technological Advances ◽

Neuroscience Research ◽

Cell Type Specific ◽

Novel Design

During the last decade, optogenetics has become an essential tool for neuroscience research due to its unrivaled feature of cell-type-specific neuromodulation. There have been several technological advances in light delivery devices. Among them, the combination of optogenetics and electrophysiology provides an opportunity for facilitating optogenetic approaches. In this study, a novel design of an optrode array was proposed for realizing optical modulation and electrophysiological recording. A 4 × 4 optrode array and five-channel recording electrodes were assembled as a disposable part, while a reusable part comprised an LED (light-emitting diode) source and a power line. After the characterization of the intensity of the light delivered at the fiber tips, in vivo animal experiment was performed with transgenic mice expressing channelrhodopsin, showing the effectiveness of optical activation and neural recording.

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Identification and characterization of cell type-specific and ubiquitous chromatin regulatory structures in the human genome

PLoS Genetics ◽

10.1371/journal.pgen.0030136.eor ◽

2005 ◽

Vol preprint (2007) ◽

pp. e136

Author(s):

Hualin Xi ◽

Hennady P Shulha ◽

Jane M Lin ◽

Teresa R Vales ◽

Yutao Fu ◽

...

Keyword(s):

Human Genome ◽

Cell Type ◽

Cell Type Specific ◽

Identification And Characterization

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Viral Strategies for Targeting the Central and Peripheral Nervous Systems

Annual Review of Neuroscience ◽

10.1146/annurev-neuro-080317-062048 ◽

2018 ◽

Vol 41 (1) ◽

pp. 323-348 ◽

Cited By ~ 39

Author(s):

Claire N. Bedbrook ◽

Benjamin E. Deverman ◽

Viviana Gradinaru

Keyword(s):

Nervous System ◽

Regulatory Elements ◽

Specific Cell ◽

Cell Type ◽

Specific Expression ◽

Recombinant Viruses ◽

Neuronal Populations ◽

Neuroscience Research ◽

Cell Type Specific Expression ◽

Cell Type Specific

Recombinant viruses allow for targeted transgene expression in specific cell populations throughout the nervous system. The adeno-associated virus (AAV) is among the most commonly used viruses for neuroscience research. Recombinant AAVs (rAAVs) are highly versatile and can package most cargo composed of desired genes within the capsid's ∼5-kb carrying capacity. Numerous regulatory elements and intersectional strategies have been validated in rAAVs to enable cell type–specific expression. rAAVs can be delivered to specific neuronal populations or globally throughout the animal. The AAV capsids have natural cell type or tissue tropism and trafficking that can be modified for increased specificity. Here, we describe recently engineered AAV capsids and associated cargo that have extended the utility of AAVs in targeting molecularly defined neurons throughout the nervous system, which will further facilitate neuronal circuit interrogation and discovery.

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Characterization of Learning, Motivation, and Visual Perception in Five Transgenic Mouse Lines Expressing GCaMP in Distinct Cell Populations

Frontiers in Behavioral Neuroscience ◽

10.3389/fnbeh.2020.00104 ◽

2020 ◽

Vol 14 ◽

Author(s):

Peter A. Groblewski ◽

Douglas R. Ollerenshaw ◽

Justin T. Kiggins ◽

Marina E. Garrett ◽

Chris Mochizuki ◽

...

Keyword(s):

Visual Perception ◽

Transgenic Mouse ◽

Learning Motivation ◽

Cell Populations ◽

Distinct Cell ◽

Mouse Lines

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Transmission of tauopathy strains is independent of their isoform composition

Nature Communications ◽

10.1038/s41467-019-13787-x ◽

2020 ◽

Vol 11 (1) ◽

Cited By ~ 13

Author(s):

Zhuohao He ◽

Jennifer D. McBride ◽

Hong Xu ◽

Lakshmi Changolkar ◽

Soo-jung Kim ◽

...

Keyword(s):

Human Brain ◽

Transgenic Mouse ◽

Mouse Line ◽

Cell Type ◽

Transgenic Mouse Line ◽

Adult Human ◽

Tau Isoforms ◽

Underlying Mechanisms ◽

Mouse Lines

AbstractThe deposition of pathological tau is a common feature in several neurodegenerative tauopathies. Although equal ratios of tau isoforms with 3 (3R) and 4 (4R) microtubule-binding repeats are expressed in the adult human brain, the pathological tau from different tauopathies have distinct isoform compositions and cell type specificities. The underlying mechanisms of tauopathies are unknown, partially due to the lack of proper models. Here, we generate a new transgenic mouse line expressing equal ratios of 3R and 4R human tau isoforms (6hTau mice). Intracerebral injections of distinct human tauopathy brain-derived tau strains into 6hTau mice recapitulate the deposition of pathological tau with distinct tau isoform compositions and cell type specificities as in human tauopathies. Moreover, through in vivo propagation of these tau strains among different mouse lines, we demonstrate that the transmission of distinct tau strains is independent of strain isoform compositions, but instead intrinsic to unique pathological conformations.

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